1. Inverted Fluorescence Microscope

Transcript

Tender No. NIRRH/Equip/2016-2017/02 SPECIFICATION OF EQUIPMENTS 1. Inverted Fluorescence Microscope The system should have following specifications: Imaging modes Bright field Phase contrast and DIC Fluorescence Switching between contrasting techniques should be automated. TL illuminator 12V, 100W Halogen lamp, Condenser filters for blue, green, diffuser, Liquid specific filters, > 23mm coded Motorized condenser turret with NA 0.5+ or better Epifluorescence system Inbuilt epifluorescence motorized 120W or more Mercury light having > 2000 hrs with fibre optic unit. Six position fluorescent filter turret Wide range of filter cubes selectively from 344nm to 800nm 1. DAPI/UV excitation (357/44nm); (Emission: 460/50) 2. GFP/Blue Excitation: 480/40; Emission: 527/30 3. RFP/Green Excitation: 540/45; Emission: 590 No overlapping of the filters to avoid loss of image quality Objectives Motorized Objective turret. 100 X Plan apochromatic WD>0.08mm(0.13), OIL (coverslip corrected, CC~0.17mm, highest NA 1.4 60 X / 63x Plan apochromatic WD>0.12 mm(0.15), OIL (coverslip corrected, CC~0.17mm, highest NA >1.40 DIC 60 X / 63x Plan Fluorite WD>2.5 mm, WIL (coverslip corrected, CC~0.17mm, NA 0.70 Phase 40 X Apochromat WD>0.2mm, OIL (coverslip corrected, CC~0.17mm, NA >1.3 DIC 40 X Plan Fluorite WD>2.5mm, with ~0.1mm coverslip, NA >0.55 Phase 20 X Plan Fluorite WD>5.0mm, with~1 mm cover glass, NA 0.40 Phase 10X Plan Fluorite Phase All objectives must have transmission in the given ranges Additional Ports Inbuilt motorized Dual port for camera system, CCD and sCMOS with motorized switching of ports for vibration free switching. Motorized / manual Magnification changer required All in one system with compact footprint Stage Automated X-Y-Z scanning stage (travel range of 115 mm × 78 mm with sub -micron resolution) ≤0.25 um Control via touch screen monitor/joystick Sample holders: to accommodate glass slides, Petridish, flasks, plates Cameras 5 mp CCD Cooled Colour Camera (>9Fps) with Peltier cooling should be quoted with an imaging software from same manufacturer as the microscope. Computer system High resolution onscreen zoom for multiple users handling without strain on eyes Direct image capture through short distance camera to avoid interference while viewing 22" touch screen monitor and core i5 computer system. Computer with 500 GB hard disk and 8 GB RAM with license copy of operating system, and high end graphic card. High resolution TFT monitor compatible with camera. Accessories Eyepiece 10x/22mm or better with Eye protection screen Low fluorescence immersion oil Dust cover Tools for system maintenance Onstage incubator for live cell imaging Stage top CO2 incubator should be capable of operating 100% CO2 supply. It should be possible to control Incubator temperature and CO2 conditions. Operating power: 100-240 VAC, 1.8 A, Frequency: 50-60 Hz, Electrical input: 24 VDC, 5 A. All electrical should be compatible with the Indian standards Free upgradable software and drivers. Software for Image Acquisition and Management. The image analysis software should have 4D experiment ability such as Advance research imaging software for acquisition and camera control through image acquisition and analysis (X,Y, Time, Multichannel), time lapse imaging, Multi-channel fluorescence, annotation, z stack imaging, time measurement and live image comparison, facility to plug in modules, 2D/3D view, filter, Morphology, Macro writing and reading, segmentation, auto measurement, Report generator facility, data base, vector layer & multi- dimensional file format 6D imaging plug (X,Y,Z, Time, wavelength, multipoint) The camera and software should be provided by the same manufacturer (of Microscope) for better compatibility with the microscope. Additional Features required: image tilling /stitching autofocus, cell counting, automated well and area scanning, Multipoint imaging module, Suitability for studying microtubule dynamics, Transfection studies with fluorescence tags The system must include minimum 5 year warranty along with software 2. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Cell Morphology Biosensor The system should have following specifications: Label free system for real time monitoring of cell behaviors like cell proliferation, permeability, extracellular matrix interactions, signal transduction, wound healing, cell migration, cell permeability, angiogenesis, apoptosis, cytotoxicity etc. User defined settings for different cell behavior parameters. Suitable for reading at least16 wells and upgradable to other format. Vendor should provide compatible arrays. Continuous measurement of cells behavior at 370C in any CO2 incubator. Should be able to measure impendance, resistance, and capacitance Attached computer system with softwares for real time monitoring, data acquisition and analysis. System should have capacity to measure impendence from 10 – 5k ohms Microelectronic sensor array should cover more than 70% area for measurement. Impendence measurement accuracy approximately ± 1.5% + 1 ohm 3. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Gel Imaging System The system should have following specifications: System should be capable of detection and quantification of Chemiluminescence and Fluorescence dyes from gels and blots. Should have user friendly and integrated software for imaging and densitometry analysis operated through single touch-screen. Excitation should be Trans-UV, epi-White; optional trans-White with diffuser board, and epi-red, epi-green and epi-blue LED modules. The system should have a large aperture lens with F-value of less than 1.0 and octagonal pixels. Detection system should have supercooled CCD camera up to -25 to -30°C with automatic focus and exposure. Image resolution should be of at least 4 megapixels or more. Maximum scanning area should be more than 15 x 20 cm System should generate 16 bit images with dynamic range of at least 4.5 fold. Image capturing modes should be automatic, semi-automatic, manual and incremental imaging without any requirement of calibration. System should have at least three emission filters each for red, green and blue fluorescence. Pixel size should be less than 11 microns 3 year comprehensive warranty Vender should provide list of equipment users and performance certificate from at least 3 users from reputed organizations in India. 4. Fluorescent Auto Imaging System The system should have following specifications: 1. 2. 3. 4. 5. 6. a) b) c) d) 7. 8. Microscope body: Inverted microscope stand including Motorized Ergonomic Stand with inbuilt Z-focus drive with minimum step resolution of 15-20 nm or better. The system should have a microscope main body mounted touch screen panel capable of controlling all motorized functions of microscope. Binocular observation tube ~28mm or better diopter included with 10X eyepieces and 22 mm field of view or higher. Objectives and motorised nose piece: 6 position motorized nose piece with 4x objective, Neo Fluoar 10X phase, Neo Fluor Phase contrast ELWD 20X Phase, Neo Fluor Phase contrast ELWD 40X Phase, 60 X/63 x 1.40 Oil or higher NA DIC objectives, Plan Apo 100X/1.40 oil or higher NA DIC Objective. Additional integrated magnification of 1.6 should be available for both camera ports as well as for eyepieces. DIC: All the components should move automatically, while DIC imaging should go out of light path while FL imaging. DIC & phase component should also be controlled through touch panel as well as software. DIC optics for 40x and 60x / 63x magnifications should be provided. Condenser: Motorized Universal Condenser suitable for all microscopy techniques such as Bright field, Phase, DIC with 7 or more slots. High precision motorized stage: High precision motorized X, Y stage and Z axis with capability to universal culture dishes, conventional slides and standard microtiter plates. Joystick/software control: With stage control and a control panel for controlling acquisition parameters like marking points, controlling bright field and fluorescence shutter, changing the fluorescence filter, snapping image, stage control. Fluorescence and bright field illumination: The system MUST have these features and controlled by company’s own proprietary software. Brightfield: LED bright field illumination for a uniform illumination and instant on - off computer controlled operation. Fluorescence: should be solid state illumination LED or similar with instant on-off mechanism c overing DAPI, FITC, Alexa 488, GFP, TRITC, Cy3, Alexa 546, Cy5, Alexa 647 or similar dyes and minimum life 10000 hrs. Fluorescence turret with minimum of 6-8 position filter cube slots for band pass (Excitation and Emission) interference. LED FL light source with following LED modules with appropriate excitation and emission band pass filters. Fluorescence LED illumination shall be control through control panel individually in nm. i. LED Module 365nm ii. LED Module 470nm iii. LED Module 590nm iv. LED Module 620nm Motorized 100:0 light sharing left & right side port. Left side port should have FOV of at least 18 mm or higher. Motorized switching for vibration free imaging. Camera: m o n o c h r o m e a n d c o l o u r c a m e r a f o r l e f t a n d r i g h t s i d e motorized ports. a) b) c) 9. 10. 11. 12. a) b) i) ii) iii) v) sCMOS Camera with 4mp (6.5 µm) or higher resolution; highest speed of 50 fps, Quantum efficiency: 80% or higher; Readout mode: Rolling shutter; Read out noise: <1 e-; pixel well depth: 30000; Linearity: 99.8%. Camera should have rolling shutter with global clear. Camera should have 12bit and 16bit mode. 5 mp CCD Cooled Colour Camera with Peltier cooling should be quoted with an imaging software from same manufacturer as the microscope. Environmental control: Stage top CO2 incubator should be capable of operating 100% CO2 supply, temperature and supply of humidifier air. The environment chamber and stage should be able to accommodate and have sample holders/inserts for petri dishes, chamber slides & well plate holders. Incubator shall be controlled through imaging software. Anti-vibration: Suitable anti-vibration plate to minimize any vibrations. Computer: Workstation for Image Acquisition and Processing with 64 bit Operating systems. Minimum computer specs as follows. Intel Xeon E5-1607v3 3.10GHz 10MB 1866 4C, 8GB DDR4-2133 (1x8GB) Reg RAM, 1TB 7200 RPM SATA 1st HDD, 9.5mm Slim SuperMulti DVDRW, NVIDIA Quadro K620 2GB 1st GFX Spl, Win 8.1 Pro 64 downgrade to Win7 Pro 64 I Should also have remote operation enable Software: The software should be company’s own proprietary software and should control all the computer controlled components of the system including the camera and Incubator should be controlled through same microscopy imaging software. Following features must be a part of the imaging software – Interactive System control with sophisticated multidimensional data acquisition visualization, analysis, image restoration, image correction and image viewing management. Data acquisition MUST have the following features – Time lapse, Z stack, Multi channel acquisition 2D and 3D deconvolution should be virtual point spread function. Colocalization analysis. 3-D reconstruction of the images, Multi point imaging, stitching. Microscope, color camera & software from a single manufacture. sCMOS camera should be control through the same imaging software. The vendor must take entire responsibility of system from installation to application support/troubleshooting. Venders should provide list of equipment users in India. 5. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Immunoassay System The system should have following specifications: Compact fully automated bench top analyzer based on principles of chemiluminescent technology. The system should perform the qualitative and quantitative analysis of hormones, cancer markers, cardiac markers, inflammation and allergy markers and other special immuno assays from serum, plasma samples and others. Built in quality control should be available on the system. System should perform at least 60 tests per hour and additional facility for STAT sample testing with continuous sample loading facility. System should have programs available on board for at least 50 different test parameters with all reagents available from manufacturer. Sample volumes should be less than 10 - 50 ul per test with flexibility of using different sample containers including primary tubes and sample cups User defined on-board sample dilution is must System to use latest mixing probe technology to mix the samples and reagents to have complete uniformity with clot detection facility System must use disposable cups and tips for all immunoassays System should have on-board refrigeration with on board monitoring of reagents. The system should have an on-board laundry system with comprehensive washing procedures. Barcode scanning for both reagents and samples should be available. System should have on-board data control work station for easy data input and test programming as well as facility to store test results up to 60 days along with USB Interface for data communication. Facility to print out patient results. Should be compatible for power supply of 220 V / 50 Hz. Warranty of at least three years with AMC/CMC quotes for maintenance to be provided. Training of personnel to be provided after installation. 6. Live Cell Imaging System 1. a) b) c) d) Inverted Frame: Fully Motorized Ergonomic Stand with inbuilt Z-focus drive Minimum step resolution of 15-20 nm or better along with 10mm or more travel range. The microscope should have both coarse and fine focusing knobs in mainframe. 1.5x and 2x mot. Magnification changer should be included. This is actually not required and cost extra e) Microscope should have automated DIC components. f) All the components should move automatically, while DIC imaging should go out of light path while FL imaging. g) The system should be capable of controlling all motorized functions including DIC of microscope. 2. a. b. c. Transmitted Light system: Transmitted light 100W or equivalent White light LED/Halogen Illumination with intensity control The system should have a built in/external fast shutter for synchronized imaging of BF/Phase Contrast/DIC with fluorescence 3. Condenser: a. Motorized Universal Condenser suitable for all microscopy techniques such as Bright field, Phase, DIC with 7 or more slots. 4. Eyepiece: a. Binocular head ~25mm or better diopter included 10X Eye piece with F.O.V 22 mm or higher eye pieces-2 nos. 5. Nosepiece: a. Six positions motorized revolving nosepiece with motorized multi position DIC turret to accommodate objective DIC prisms of different magnifications. 6. Imaging Ports: a. The Microscope body should have motorized 100:0 light sharing b. Left side port should have FOV of at least 18 mm or higher field of view for the bigger chip size cameras like sCMOS 7. Stage: a. Motorized Encoded X-Y Stage with multiple holders to adapt Petri dishes, Multi Well plates and glass slides. Preferably with a warmer with variable temperature control and temperature indicator 8. Objectives: a. High numerical aperture objectives suitable for bright field, fluorescence, DIC and phase. b. Objectives - 4x, Plan Apo, 10X Ph, Plan Apo, 20 Ph, Plan Apo/flourite DIC 40X/1.30 Oil & Plan Apo 100X/1.40 ~0.7 Oil or higher Plan Apo. 9. Fluorescence Module: a. Fluorescence turret with minimum of 6-8 position filter cube slots for band pass (Excitation and Emission) interference b. LED FL light source with following LED modules with appropriate excitation and emission band pass filters i. LED Module 365nm ii. LED Module 470nm iii. LED Module 590nm iv. LED Module 620nm 10. Fluorescence Light Source: LED fluorescence light with minimum 10,000 hrs life. It should have separate controller unit to control individual LED line. 11. Camera: EM CCD Back illuminated high sensitive camera higher suitable for high quality microscopic images for bright filed, DIC and multicolor & low light fluorescence. a. High speed Cooled (-20oC or better) Color & Monochromatic digital USB3 camera with CCD sensor 3 million pixels monochrome mode (1920 x 1440; 4.54 µm x 4.54 µm) pixel size, 5.6 million pixels for colour mode b. The camera should be with high speed as 40 fps or better with full frame and maximum speed shall be 100 fps or better with color and monochrome mode. c. quantum efficiency of 95% d. Specify cooling mechanism, stabilized temperature and quantum efficiency e. Company can quote two separate CCD Cooled camera’s to cater monochrome and colour imaging application. sCMOS f. Camera with 4mp (6.5 µm) or higher resolution; g. Highest speed of 55 fps h. Quantum efficiency: 70%; Readout mode: Rolling shutter; Read out noise: <1 e-; i. dark current 0.14 eˉ/p/s.; pixel well depth: 30000; Linearity: 99.8%. j. Camera should have rolling shutter with global clear. k. Camera should have 12bit and 16bit mode. (quote separately for both the cameras) 12. a. b. 13. a. Focus drift compensator Focus drift compensator for focus detection and tracking function. One Click or continues by via the touch panel independent of software. The focus search function supported by a cell-safe, near-infrared LED/laser enabling instant focusing on samples even at high magnification. Software and computer : Imaging Software to control all motorized Microscope components, Camera for acquisition of images in Z- Series with autofocus, Multi-Channel imaging, 3D reconstruction, Time Lapse imaging. Should be equipped with measurement analysis module for spatial and morphological segmentation at individual channel. Software b. c. 1. 2. 3. 4. 5. 6. 7. 8. 9. 14. should be able to export statistics data and graph. Data processing unit: Branded with Xenon processor with 16 GB RAM, DVD Writer, 1TB GB or higher HDD, Original Window 7 Operating System (64 Bit), Original Anti Virus with CD, LCD Monitor 23-24 inches with color printer shall be quoted. Dedicated point spread function based deconvolution software with following features. Dedicated 2D & 3D Deconvolution software with Extensive Image Parameter checking feature Should create image histograms Automatic estimation of the background Automatic deconvolution of time series Should handle multiple file formats Automatic and manual cropping of datasets in five dimensions Easy comparison between different slices and original and deconvoluted images Maximum Intensity Projection (MIP) renderer & Volume Renderer which should produce physically realistic 3D representation of data. CO2 Incubator: Box Type / Cage Type black or dark CO2 incubator should be capable of operating 100% CO2 supply which can support long term experiments such as 72 hours or more. The environment chamber and stage should be able to accommodate and have sample holders/inserts for petri dishes, chamber slides & well plate holders. 95% Humidity, 0–600C temperature control variable with 0.10C. All the parameters such as Co2 flow temperature and humidity should be programmed by same imaging software. Also the chamber should have LED illumination inside. Note: Please quote separately for each item. 7. Digital Droplet PCR Overall features 1. System should provide an absolute measure of target nucleic acid molecules with high precision and sensitivity without the use of standards and standard curves. 2. System should support multiplexing experiments with probe base as well as dye base chemistry. 3. A single party must provide all the components and should be a single workflow The system should have following components 1. Droplet generator 2. Plate sealer 3. PCR machine 4. Droplet reader 5. Software 1. Droplet generator a. System should be able to partition a 20 ul or more of sample volume into 1 Nano liter sized partition b. System should have a partition generator capable of generating partition for upto 96 samples in less than 30 minutes 2. Plate sealer 3. PCR Machine a. A stand alone gradient 96 well PCR b. System should come along with gradient 96 well PCR which can be used as a standalone PCR machine as well. 4. Droplet reader a. b. c. d. e. f. g. h. A two color fluorescent partition reader capable of reading 96 samples at a time and a suitable software . System should be capable of analyzing 96 samples at a time. System should offered precision of ± 10% System should have a linear dynamic range of five orders of magnitude for detection of input samples. System should be able to detect and quantify one copy of target sequence. System should use two light emitting diodes for illumination and differentially detect emission using two filtered multipixel photon counter. The reader should be able to detect at least 2 colours from a single partition. The reader must be sensitive to detect fluorescence from amplification of a single target molecule. 5. Software: a. Should provide fraction of negatives droplets for each samples to fit to a poisson algorithm to determine absolute copy number of target DNA. b. Should have the ability to count the number of partitions for each sample after every run. Should give concentration analysis for each sample. c. Should have copy number variation, Gene expression, Absolute Quantification & Rare event analysis modes. d. System should be capable of absolute quantification, rare mutation and sequences detection, SNP detection, copy number variation, gene expression analysis, single gene expression, NGS validation and library quantification. e. Absolute quantification: The machine should be able to quantify nucleic acid molecules (both DNA and RNA) in the sample without the necessity of standards. f. The machine should be able to detect a single copy of the target in the sample. g. Rare mutation detection: The machine should be able to detect a single mutant copy in a background of a million wild type copies in a single run, using dyes, single probe or dual probes. General requirements a. The supplier should also be able to provide inventoried and custom designed prevalidated assays for the templates of interest. b. All the above features must be supported by product/technical brochures, highlighted on Point-to-point basis will be only considered while technical evaluation c. System should be supplied with all Essential Accessories and reagents. 8. Confocal Microscope The confocal microscope system should be of latest model and modular technology suitable for biological applications. The confocal system should be capable of high sensitivity detection mechanism meeting various applications of Biological samples including multichannel fluorescence imaging with Z-stack, Live Cell imaging, time lapse applications including co localization, FRAP, FRET & FLIP. It should be Multiphoton upgradable. The system should be offered with the following configuration: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Fully motorized inverted microscope for Bright field, Fluorescence and DIC preferably with dedicated touch screen TFT display for controlling motorized components of the microscope Motorized Z-focus drive with minimum z-step resolution of 25 nm or better with dedicated TFT/LCD screen for the control of motorized functions of microscope 6 position motorized FL filter wheel & 6 position motorized nosepiece Computer controlled continues correction of Z drift with wave length above 800nm Laser/LED based system Motorized X-Y scanning specimen stage with universal sample holder for slides and 35/60mm petridish Piezo/Galvo stage with 3-5nm step size. 12V /100w halogen illumination for transmitted light and high power LED. Metal Halide/ Mercury Arc lamp 120/130W illumination for Fluorescence with lamp of life minimum 2000 Hrs. High resolution Plan Apochromat Confocal Grade objectives 10x/0.4, 20x/0.70, 40x/0.85, 60/63x/1.40 oil 100X 1.4 oil with DIC prisms for all objectives. Fluorescent bandpass filters for DAPI, FITC/GFP and TRITC/Rhodamine. High sensitivity confocal laser scanning and detection unit with built-in spectral detectors for high efficient fluorescence signal collection. All detectors should be capable of working in intensity and spectral mode imaging Scan head should have at least 4-5 independent filter-free inbuilt spectral detectors with independent voltage and offset control. Out of 4-5 detectors, at least two fluorescence dyes to be imaged in high sensitive built in spectral GaAsP/Hyd detector with more than 40% QE. Confocal system with upgrade option to simultaneously image and separate higher number of fluorochromes would be preferred. All the FL spectral detectors should be built in inside scan head and should be with filter free with freely selectable emission band width detection capability to suit to the emission spectra of the dyes. The spectral dispersion of the emission light should be based on either reflection grating with enhanced/improved spectral signal collection device or with prism based spectral dispersion with high efficiency. The system should be capable of recording emission spectra with minimum spectral resolution of 5nm or better. Computer controlled continuously variable confocal pinhole with software control. Maximum scan resolution with spectral detectors should be at least 4Kx4K for all channels and higher will be preferred. The scan field diagonal should be at least 18-20 mm F.O.V. higher will be preferred Scan Zoom range 1:48x or more and should be adjustable in steps of 0.1 19. 20. 21. 23. 24. 25. 26. 27. 28. 29. 30. 31. Spectral System should be capable of acquiring minimum 5 or higher frames per second @ 512x512 pixel resolution. It should be able to perform fast dynamic live cell time lapse imaging with a high speed of 110fps or better @ 512x16 resolution. Digitization capability of 8/12/16 bit should be available with the system. An additional transmitted light detector should be offered for bright field and DIC imaging. Visible Laser Set with AOTF containing the laser lines: 458,488 & 514nm with 30mW DPSS 561nm with 20mW, 633nm with 10mW. 594nm laser UV -405 nm with ROI imaging capability. Latest control computer with Software capable of controlling Motorized functions of microscope, digital camera, scan head control, laser control including AOTF and Image acquisition & processing. Workstation with Windows 7 Professional (64 bit) operating system. - Intel Xeon Quad core E5-1620 V3 3.5 GHz 10MB - 32 GByte RAM - NVIDIA Quadro K620 1GB high performance GPU - 3 TByte SATA hard disc drive - 9.5mm Slim SuperMulti DVDRW 1st ODD - USB 3.0/2.0, IEEE 1394 A/B - Keyboard and mouse Ultra-HD 31" LED Monitor (AH-IPS), GB-r-LED backlight for extended color space, 4096 x 2160 pixels (4K) . Basic software for microscope control, Confocal system control, with basic modules for ROI scan and bleach for FRAP, 3D imaging and reconstruction, time-lapse, co-localization etc. Application software for Advanced 3-D software, Advanced FRET AB, FRET SE, FRAP, and FAST FRAP software. Multi point imaging facility to be offered. Anti Vibration Table should be included with the system from the same manufacturer of Confocal. Suitable 6KVA UPS should be supplied with the system A complete automated environmental control chamber (incubator) for long term live cell time lapse imaging with temperature, CO2 and Humidity control. The system should be able to use 100% CO2 gas supply and to provide 5% pre-heated CO2 gas to the chamber. Should be compatible with piezo/galvo stage. Offline software with advanced 3d construction, renduring, processing , automated 2d analysis including automated counting, mesearments, FRET,FRAP etc. with HP/Dell workstation with 32 inch monitor. 9. Multimode Monochromator Based Microplate Reader 1. The system should be capable of measuring UV-visible absorbance, fluorescence, luminescence in end-point, kinetic and spectral scanning modes. The system should have quadruple monochromators for wavelength selection, two each for excitation and emission. The system should be capable of reading 6, 12, 24, 48, 96 and 384-well plates with or without lids. The temperature control of the system should be from 4-5°C above ambient up to 4245°C with temperature uniformity of less than 1°C and accuracy of  1°C or less at 37°C. The system should have linear, orbital, double orbital plate shaking modes with programmable speed and duration. Single integrated windows based software for data acquisition, analysis & management with at least 4 user licenses should be supplied with the instrument. The software should be able to analyze the data and perform the calculations. The light source should be a Xenon flash lamp (1 joule/flash) with a life of at least 1 billion flashes. In the absorbance mode, the wavelength range should be at least 230 nm to 999 nm with an increment of 1 nm and a bandwidth between 4 to 12 nm. The dynamic range in absorbance mode should be from 0-4 OD  2% or less with a resolution of 0.001 OD or better. The monochromator wavelength accuracy and repeatability should be  2 nm and 0.2 nm respectively. The measurement speed for 96 and 384 well plates should not be more than 20 seconds and 50 seconds respectively. Pathlength correction facility should be available as a standard feature. In the fluorescence mode, the wavelength range should be at least 250 nm to 600 nm with dual grating monochromators (top and bottom) with 1 nm increments. The dynamic range in fluorescence mode should be at least 5 decades with a sensitivity of at least 3 fmol fluorescein for top reading and 5 fmol fluorescein for bottom reading. The system should be capable of performing time resolved fluorescence measurements. In the luminescence mode, the wavelength range should be at least 300 nm to 600 nm with a sensitivity of 20 attomol or lower and a dynamic range of at least 6 decades. The power requirements of the instrument should be as follows: 100-240 VAC 50/60 Hz. 130 Watts max. The instrument should be CE and NRTL Safety Agency marked. The instrument should be supplied with computer having latest Configuration. Warranty period 3 yrs. plus 2 years AMC. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 10. Optical Microscope with in built Camera and Software: The instrument should have the following features  Colour Corrected Infinity Optical System  Trinocular Eyepiece tube, with Built in Camera  CMOS chip with minimum 3.0 mega pixel & Progressive scanning system  30 deg inclined Siedentopf Type with interpupillary distance 48-75mm  Eyepiece 10X / 20mm or 22mm with diopter adjustment on eyepieces & rubber eyecups  Built in transmitted 6v/30w Halogen  Reversed Quadruple or Quintuple nosepiece  Microscope Stand with coaxial fine and coarse focusing mechanism Coarse motion Torque adjustable, upper stage drive stop incorporated  Rectangular Mechanical Stage –in x & y direction with vernier scale, right hand control  N.A. 1.25 Abbe Condenser with slider slot for Phase.  Calibration slide with 4 circles(1.5mm,0.6mm, 0.15mm &0.07mm) & 1000um stage Micrometer to calibrate microscope with software & Traceability Certificate  EF-N Plan Objective 4X, 10x, 40x (SL), 100x (Oil, SL)  Blue filter 45mm & Vinyl Dust Cover  Upgradeable to E-LED Fluorescence attachment, EPI-Illuminator, Polarising & Phase contrast attachments.  The imaging software should have the following features - Real Time Images, Image capturing & Storage of Images - Video recording & Programmable interval shots - Automatic count evaluate cell quantity with Calculation tool - Measurement in microns, inches, millimeters Length, Circle, Ellipse, Rectangle, Irregular - Shape, Perimeter, Radius, Circumference, Angle Measurements - Measurement Table - Grid, Scale Bar, Cross scale options for Real time images - Date & Time stamp on the Captured Images & Videos - Options to adjust brightness & contrast, colour corrections, etc - Options to Save, open, prints & White Balance - Image data with automatic ref. number, date & time - Compatible with OS like Windows 7, 8, Mac, Linux  Computer with latest Configuration  Warranty period 3 yrs. plus 2 years AMC 11. Five Part Differential Hematology Analyzer The system should have following specifications: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Five-part differential hematology analyzer for mice, rats, rabbits, marmoset, monkey etc. and should have add on unlimited custom profiles for other species Should be able to analyze 22 to 28 parameters with minimum requirement of reagents and able to generate histogram and scattergrams for WBC, RBC and PLT and differential WBCs. Should be less than 20 kg in weight, space-saving with large touch-screen LED/TFT display. Should have integrated self-cleaning system with cyanide free reagents Should be able to do Automatic or manual calibration. Should be able to give accurate reading in minimum 20μL and maximum 50μL of sample. Should be validated for peritoneal and synovial fluids from laboratory animals. Should be able to generate accurate and reproducible results comparable to references. Should be able to process minimum 30-50 samples/hour Should work on standard power 100V-240V with 50Hz/60Hz Should stores 1000-5,000 records in database with simplicity, flexibility and usability with easy loading of software upgrades or archiving of data Should have in built thermal printer as well as interface for printer attachment Should have Serial port, USB facility for interface. Should work in Operating environment Temperature: 15-30°C Relative humidity: max 80% (noncondensing) 12. Low Volume Nitrogen Evaporator  Should be having Dimensions: Width-12-15 inch max. Height (lid down) – 10-14 inch max. Height (lid up) – 25-32 inch max. Depth – 20-25 inch max from tip of nose to rear of exhaust Should have Weight – Less than 40 lbs Electrical Requirements *Line Voltage – 100 to 120 VAC or 200 to 240 VAC 50/60 Hz with automatic selection for 115 VAC operation *Line Frequency – 50/60 Hz Gas Requirements Built-in nitrogen regulator. Should use clean, dry, nitrogen regulated to 60 psig minimum, 100 psig maximum (4. 13-6.89 bar) while flowing *Gas flow of 140 L/min for 24 psi at 54 nozzles Environmental Ambient Temperature: 5 to 35oC Ambient Humidity: 5 to 85% relative humidity non-condensing      Audible alarm when the water bath level is low and heater disabled to prevent damage Indication when the gas pressure is low        Controlled water bath adjustable from ambient to 80oC Different interchangeable racks available for tube sizes from 1.5 mL to 30mL LED display for better visibility under fume hood Convenient size --no hood space required Exhaust open to atmosphere or within Fume Hood Easy to use water drain pump Unattended operation for up to 50 samples 13. PCR Machine (Thermal Cycler) The system should have following specifications: Features Specifications Block format 224-48 well: 0.2-0.5ml volume OR (Independently 324-32 well: 0.2-0.5ml volume controlled) Flexibility of interchangeable blocks Yes Gradient Block Yes Gradient range 30ºC-99ºC system with maximum gradient of 1ºC Must offer at least 12 rows of gradient Block temperature range 40C to 1000C Temperature accuracy +/- 0.2ºC Temperature uniformity +/- 0.4ºC Reaction volume 10-100 l Gradient calculator Calculated or Block Adjustable heated lid temperature Yes Over temperature cut off Yes Maximum ramp rate 5ºC per second Auto restart on power failure Yes User Inter face Touch screen for easy navigation Memory 10,000 protocols at least Voltage 50/60 Hz Password protected Yes Comprehensive warranty of three years Yes 14. Clinical Analyzer The system should have following specifications: 1. Fully automated random access benchtop clinical chemistry analyzer capable of performing the analysis of a wide range of analytes from serum, plasma, and other body fluids. 2. Open system flexibility with high throughput of at least 60 tests per hour. 3. It should be able to perform End-Point, Rate Assay, Point to point assays to enable all routine and special assay types 4. Open channels for custom chemistry parameters required for non-routine or research applications 5. Should be based on analytical principles of absorbance photometry/turbidometry. 6. Calibration should be Linear, Non Linear & Multipoint. 7. Large range wavelength should be available by means of halogen/Xenon lamp source . 8. There should be atleast 30 or more on board programs with capacity to run 20 tests at a time. 9. It should be capable of delivering 5-50 μl sample volume with suitable flexibility to utilize different sample containers including primary tubes 5-10 ml sample cups, micro cups which should be disposable or semi-disposable. 10. Sample loading should be enabled in continuous loading format with sophisticated washing procedures for washing of probes/cuvettes etc. ensuring result integrity. 11. Probe must have liquid level sensor with built-in crash detection to eliminate errors in analysis. 12. Facility of sample dilution should be available onboard. 13. Sample flagging for errors with system ability to re-runs with diluted sample or changed volume of sample would be preferable. 14. It should have facility of on-board cooling with on/off control. 15. Should have an interactive built in QC software with data being available upto 2 months with facility for calibration reports. 16. The system should also have onboard data storage facility. Complete backup of system database should be possible. 17. The analyzer must be provided PC with LCD Screen, Minimum 500GB Hard Disk, CDROM, Keyboard, mouse and Compatible on line UPS with 30mins back up. 18. Power supply of system to be 220 V / 50 Hz. 19. The consumable washing and flow-cleaning system consumable solutions must be lowcost and their detailed composition must be stated in technical bid for proper scrutiny. 20. The manufacturer should have relevant ISO certification and products should be accredited by agency like FDA/European CE/UL. The relevant certificates must be submitted in the tender. 21. Warranty period should be of three year. Quotes for AMC/CMC should be mentioned for the following 5 years. List of all spare parts with costing also to be supplied. 22. Personnel’s training after installation is mandatory. 15. UV/VIS Spectrophotometer The system should have following specifications: Optical system Double beam type with variable band width Light Source D2 Lamp & Halogen lamp Wavelength Range 190 to 900 nm Wavelength Accuracy ±1 nm Wavelength Repeatability ±0.1 nm Stray light 0.005 % at 340 nm Spectral bandwidth Variable bandwidth 0.1nm to 5nm Photometric Range -4 to 4 Abs Detector Photomultiplier tube Photometric display ± 9.9999 Abs Photometric accuracy ± 0.015 Abs Power requirements Should be compatible for power supply of 240 V / 50 Hz. Cuvette The system should be also able to use low (100l) volume cuvettes of any path – length One drop measurement Accessory to allow one drop measurement should be present Cell holder (microcell holder) Option for changing cuvettes holder to accommodate 100l to 3ml cuvettes Connectivity It should have USB for PC connection. Software System with compatible data analysis software Computer (Desktop/ Laptop) Core i5 computer system. Computer with 500 GB hard disk and 8 GB RAM with license copy of operating system, and high end graphic card. 16. Blood Pressure Monitoring System for Animals  Capability to measure all 6 blood pressure parameters; Systolic BP, Diastolic BP, Mean BP, Heart Rate, Blood Flow, and Blood Volume. Should use a Clinically Validated Volume Pressure Recording (VPR) tail-cuff technology to obtain accurate, consistent and reproducible blood pressure measurements. Systolic or Diastolic blood pressure should be actual measured value Ability to measure both rodent subjects; mice and rats. Ability to measure mice as small as 8 grams to rats as large as 950 grams. Should have the ability to display all the blood pressure measurements and heart rate on a built-in Colour LCD Display without the need for a separate computer. Integrated homoeostatic warming blanket/ chamber should be available to maintain the animal’s proper body temperature for accurate and consistent blood pressure measurements. Animal Holder with nose cone should be provided.        17. Nanophotometer   Absorbance range: 0.01 – 1.5 (10 mm equivalent: 0.05 – 375) Detection range: dsDNA: from 2 ng/µl to 18,750 ng/µl, BSA: from 0.08 mg/ml to 543 mg/ml Minimum sample volume: 0.5 μl Path lengths: 0.04 mm, 0.1 mm, 0.2 mm, 1 mm and 2 mm Virtual dilution factors: 5, 10, 50, 100 and 250 Wavelength range: 180 – 1000 nm Wavelength scan range: 200 – 950 nm System start up time: Less than 5 seconds, warm up shouldn’t be required Wavelength reproducibility: < ± 0.2 nm Wavelength accuracy: ± 2nm Measure time for full scan range: 3.5 seconds Absorbance reproducibility: ±0.003 A (0 to 0.5 A), ±0.007 A (0.5-1.0 A) @ 260 nm Absorbance accuracy: ±0.005 A or ±1% of the reading Lamp: Xenon flash lamp Lifetime: min 10 years Display format: 320 x 230 pixels Size: 150 mm x 270 mm x 350 mm Weight: less than 5 kg Performance verification: Auto diagnostics when switched on Operating voltage: 100-250 V, 50 Hz, Max 30 VA                   18. UV-Visible Spectrophotometer with Temperature Control (Spectrophotometer Assembly) (1 No.) The system should have following specifications: To measure the concentration and purity of nucleic acid and protein samples and cell density at a broad range of sample volumes (approx 2 ul to 2 ml ) including 96 well microplates . Microplate types Detection mode Cuvette Port It should be able to read 6, 12, 24, 48, 96 and 384-well microplates Absorbance It should have dedicated cuvette port and should be able to read standard 10mm pathlength cuvette. Other Labware It should also be compatible to read 2 µL low volume microplate accessory with 16- sample microspots for direct nucleic acid quantification and other applications. Wavelength range Read methods 190-900 nm Should be able to perform end point, kinetic, spectral scanning and well areascanning Temperature control Ambient +4°C to 65°C. Temperature control should be by Natural Convection to prevent edge effects. Must have temperature gradient setting to prevent condensation in lidded plates Spectral bandwidth Wavelength Selection Variable bandwidth 0.5nm to 5nm It should have tunable grating Monochromator for wavelength selection. Should be able to select Wavelength in 1 nm or greater increments ± 2 nm 0.0-4.0 OD ± 0.2 nm Wavelength accuracy Dynamic range Wavelength reproducibility Resolution Light source Stray light Detector Power requirements Quartz cuvettes Temperature control Built in shaking for microplates Connectivity Power Connectivity Power 0.0001 OD Long- life stabilized xenon / D2 lamp 0.03% at 230 nm PMT detector 100 - 240 VAC+ 10% 50/60 HZ , 120 VA For 50 l to 2 ml volume range Ambient +5 to 45°C Linear , orbital , double orbital It should have USB for PC connection. 100 - 240 VAC 50/60 Hz It should have USB for PC connection. 100 - 240 VAC 50/60 Hz Compatible software/s Computer (Desktop/ Laptop) Data Analysis software should be supplied with the instrument. Should be able to perform data analysis without need to export readings into excel. Core i5 computer system. Computer with 500 GB hard disk and 8 GB RAM with license copy of operating system, and high end graphic card. 19A. Deepfreezer (–80oC)( 3 Nos.) The system should have following specifications: 1. Vertical type, approximate capacity of 450 to 550 liters in the temperature range of -80 to -86OC. 2. Approximately Dimensions (L x W x H) Exterior 35 x 30 x 75 inches Interior 25 x 20 x 50 inches 3. Microprocessor controlled, eye level digital LED display with data logging and recording system. 4. Audio visual high and low temperature alarm 5. Quicker temperature recovery after door opening 6. Interiors should be stainless steel with rounded corners for easy cleaning 7. Electrical system should be automatic low voltage surge protection with compatible voltage stabilizer (5) KVA of ISI mark 8. Should have solid stainless steel shelves for creating 5-6 independent interior compartment with doors. 9. Should have sturdy door lock integrated key with handle for single hand operation. 10. Castors for easy mobility 11. Warranty for 3 years on the Freezer & additional 2 years on compressors. 12. Should have ISO certification 19 B. Deepfreezer (-80oC) (1 No.) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Ultra low temperature freezer Vertical type Dimensions 78"H x 27"W x 37.6"D Interior 51.23"H x 17.78"W x 28.29"D Temperature range upto -85 +/-5 deg C Audio visual high & low temperature alarms Microprocessor based temperature control Control & data logging in TFT screen interface DATA recording & viewing (tabular & graph) of minimum one year Rechargeable battery back for control panel. Four or five inner compartments with perforated trays and individual doors. Stainless Steel interiors Outer made of GI sheet with bacteria resistant coating Cacade refrigeration system Castors for easy mobility ISO certification Anti-condensation to prevent moisture on the door, walls. Compatible voltage stabilizer (5) KVA of standard brand/ISI mark 20. Refrigerated Table top Centrifuge (2 Nos.) The system should have following specifications: Maximum Speed: 5000rpm (For Swinging bucket rotor), 10000 rpm (For fixed angle rotor with 50 ml capacity) and 14000rpm (for fixed angle rotor for microtubes of 1.5ml or 2ml capacity) Rotor Capacity: 4 X 175-180 ml (Swinging bucket) 6-8 X 50 ml (Fixed angle) 24-30 X 2ml (fixed angle) Maximum RCF: 5000 X g (for swinging bucket rotor), 15000 X g (for Fixed angle rotor with 50 ml capacity) and 21000 - 25000 X g (for fixed angle rotor for microtubes) Rotor with accessories: Swinging bucket rotor having radius ~15-18cm with Biocontainment Certification including 4 Round Buckets with 4 Biocontainment lids for the buckets and 4 spare O-rings; Adapters (6 each) for 50 ml tubes (capacity 4 X 50 ml) and 15ml conical tubes ( capacity 16 – 24 X 15ml) Fixed angle rotor (angle of 45o) for 50 ml (6-8 tubes) with Biocontainment certification Voltage: Hertz: Temperature range: Timer: Display: Optional rotor Fixed angle rotor (angle of 450) for microtubes (1.5ml-2 ml) having extra space to accommodate 2ml filter columns for nucleic acid purification 230V 50/60 Hz -40C to +400C 99hr 59min, continuous Digital Fixed angle rotor(angle of 450) for 15 ml tubes (8-20 tubes) 21. Gel Documentation System (2 Nos.) The system should have following specifications: Hardware 1. 2. 3. 4. 5. 6. 7. 8. 9. Image Resolution: System should have Image resolution >4 mega pixels for resolving closely spaced bands on a gel or blot. Range: System should have 4.6 x 4.6 μm pixel size & >3.0 orders of linear dynamic range Automation: System should be completely automatic & user does not have to zoom, focus, adjust aperture or select light source. Sample Trays: System should be modular with different sample trays & flexible to image a wide variety of applications, including nucleic acid, visible dyes, SYBR safe and Stain Free Gels. Sample trays should be customizable per user and recognized automatically. Should have lens flat-fielding calibration for each sample tray to deliver image data that are always optimized and reproducible without imaging artifacts, providing superior image uniformity and quantitation System should have Stain-Free capability for stain-free gels and blots. Lights System should have UV, White light, & optional Blue light. Filters System should require only one emission filter to accommodate a large portfolio of detection methods: ethidium bromide, SYBR® Green, SYBR® Safe, SYBR® Gold, GelGreen, GelRed, Fast Blast™, SYPRO Ruby, Flamingo™, Oriole™, CY3, rhodamine, Green fluorescent protein, Hoechst, Krypton, silver stain, copper stain, zinc stain, Coomassie Brilliant Blue, Coomassie Fluor Orange, and other spectrally similar stains, labels, and dyes. Size: System should be compact with small footprint and maximum size of WxLxH of 27 x 44x 38 cm. Software 1. Software should have highest level of automation in hardware calibration, image optimization, capture, and analysis. 2. Should have automated workflow recorded in a protocol file from image capture to results thus eliminating need for training. 3. Should allow 100% repeatability of the workflow by any user and ensures optimized image data and analysis from a gel in a single uninterrupted, fast, and completely reproducible workflow. 4. Should have automated image capture driven by a selected gel or blot application. 5. Should have one-button acquisition from image capture to result. 6. Should generate the publication ready images (dpi, dimension and format) with one click export option. 7. Should generate customizable reports. 8. Should have feature for Automatic print when only imaging and printing is required. 9. Software should have easy copy/paste functionality, crop, zoom, 3D and colors. 10. Should come with suitable computer. 22. Homogenizer (2 Nos.) The system should have following specifications: 1. Digital handheld homogenizer with rotor stator technology and brushless wear free motor 2. Should be able to homogenize solid, liquid and emulsion samples to uniform suspension. 3. Working sample volume approximately 0.05 ml -1000 ml 4. Power supply- 100 W (input) and supply Voltage- 230, at 50 Hz. 5. Noise level less than 70 dB at approx.. 30,000 rpm. 6. Temp: - Ambient 0-40 0C 7. Variable speeds from approx. 5000 to 30,000 rpm with speed regulation. 8. Digital display for speed 9. Superior grade electropolished stainless steel, autoclavable and easy to clean probes for homogenizing in different volumes (0.1 - 5 ml, 0.3 - 10 ml, 2 -250 ml) 10. Stand including holder for probes and toolset 23. Rapid Homogenizer System The system should have following specifications: 1) The system should be capable of rapidly homogenizing microbial cell pellets, fecal material etc.as well as soft and hard tissues for downstream applications such as genomic DNA, RNA and protein extraction. 2) It should be compatible with any lysing matrix or kits for DNA/RNA/Protein extraction. 3) The system should also be capable of simultaneously homogenizing multiple samples. 4) The sample capacity for homogenization should be at least 2 ml or more. 5) The homogenization speed attained by the device should be at least 5.5 m/s or better. 6) It should be possible to carry out homogenization at 4° C. 7) The system should have a small footprint. 8) The power requirements of the instrument should be as follows: 100-240 VAC 50/60 Hz. 130 Watts max. 9) The instrument should be CE certified. 10) Warranty period 3 Yrs. plus 2 Years AMC.