Transcript
Lisboa, July, 4-6
Bioelectronics A.G. Mestre, P.C. Inácio, Luís Alcácer, Fabio Biscarini Maria C. R. Medeiros and Henrique L. Gomes BASIC SCIENCES AND ENABLING TECHNOLOGIES Organic Electronics- LX © 2005, it - instituto de telecomunicações. Todos os direitos reservados.
Outline • Motivation • Bioelectronic systems in vitro • Organ-on-chip • Electrical activity of tumour • Fundamental research on cell cultures
• Implantable bioelectronics. • Implant for a spinal cord-injury
• Conclusions
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Motivation To develop bidirectional sensing devices to interact with living cells and tissues. cell Bi-directional communication Organic semiconductors, biocompatible and biodegradable substrates
Flexible array of sensors, (Takao Someya group)
Electronic Interface 3
Sensing devices
Polymer electrodes produced by inkjet printing
Schematic diagram of the recording system.
Conventional MEA (gold electrodes on silicon) Sensing device connected with a commercial Petri-dish. 4
Organ-on-chip (a)
(b)
Zebrafish heart on (a) gold (b) conducting electrodes
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Small organs are important research tools in fundamental studies of drug discovery and safety pharmacology.
Organ-on-chip
Embryoid body with autonomous cardiac contractile cells
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Electrochemically gated field effect transistors(EGFET) Gate __________ ++++++++++ Electrolyte __________ ++++++++++ Semiconductor Source Drain
Cross section view of EGFET
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Graphene based transistors
Source
Drain
In collaboration with: The International Iberian Nanotechnology Laboratory
Gate
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Graphene based transistors Gate Drain Source Cardio cells
In collaboration with: The International Iberian Nanotechnology Laboratory
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[email protected]
Cancer cells
Glioma cells
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Bioelectrical activity of cancer cells
Addition of antibiotic
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Bioelectrical activity of cancer cells 10 -9
Electrode with active cells
Electrode with active cells 10
-10
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-12
10
-13
10
-14
Electrode with silent cells
V
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S (V /Hz)
10 -11
Bare electrode
Bare electrode 10
-15
10 -2
10 -1
Frequency (Hz)
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10 0
Bio-electrical Signals
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Signal analysis
7/6/2016
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Implantable devices Active Multifunctional Implantable Device (AMID) to treat Spinal Cord Injury (SCI). • Flexible, conformable to the injury site • Organic electronics and microfluidics integrated • Biocompatible, largely biodegradable, life time tailored to 4-6 months
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Nano-fibrous bacterial cellulose
Bacterial Cellulose is produced by bacteria Gluconacetobacter sacchari
After compression between plates at room temperature, the water is removed.
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1 cm
PLGA Poly(lactic-co-glycolic acid) (mechanical and stress responses)
Bending radius down to 80 µm
Poly(lactic-co-glycolic acid) is a Copolymer which is used in a host of Food and Drug Administration (FDA) approved therapeutic owing to its biodegradability and biocompatibility
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Active Multifunctional Implantable Device (AMID)
Transistors
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Active Multifunctional Implantable Device
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Implanted AMID device in contusion SCI animal model (acute)
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Demonstration of implanted device in contusion SCI animal model Schematic view of the surgical technique for the AMID implantation in vivo Implantation of the AMID in the mouse spinal cord
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Conclusions • Large area printed polymer electrodes are suited to fabricate organ-on-chip systems. • Simple planar sensing devices can be fabricated in glass or in biocompatible substrates such as bacterial cellulose. • We have demonstrated the applicability of these printed electrodes to measure in vitro a population of contractile cells(cardiomyocytes) and quasi-periodic oscillations in Glioma cells.
• A flexible biocompatible active multifunctional device was developed to repair spinal cord injury.
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Acknowledgements
Ana Mestre
Qian chen
Profª Maria Medeiros
Profª Leonor Cancela
Asal Kiazadeh
Joana Canudo
Pedro Inácio
Prof. Fabio Biscarini (Unimore, Modena, Italy)
Thank you for your attention! 23
Bologna (Italy) 8-10 June 2016
