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Antibodies for Cancer research fra CST, Nyt NEB katalog med fine giveaways, L7 hPSC Culture System fra Lonza samt nye produkter fra NEB
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Stem Cell Transfection using Nucleofector™ Technology
L7™ hiPSC Reprogramming and hPSC Culture System
Transfection of stem cells is typically a very difficult task using non-viral methods. With the Nucleofector™ Technology stem cells can be consistently transfected at high efficiency for various applications, comprising those that require co-transfection of several substrates:
The L7™ hPSC Culture System is a robust culture system that supports every other day feeding of pluripotent stem cells under defined and xeno-free conditions. This xeno-free, fully defined system includes a medium, matrix, passaging and cryosolution. L7™ is a complete system for the maintenance and expansion of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
• Proven for genome editing in ESCs or iPSCs using ZFN, TALEN, and CRISPR systems • Efficient episomal reprogramming of PBMCs, fibroblasts, CD34+ or other cells into iPSCs • Optimized, ready-to-use transfection protocols for primary adult stem cells such as mesenchymal stem cells (MSCs)or neural stem cells (NSCs)
• Reliable reprogramming of peripheral blood mononuclear cells (PBMCs) into iPSCs • Robust culture system that supports every other day feeding of pluripotent stem cells • Efficient differentiation into all three germ layers • Easy transition to clinical applications xeno-free, defined
View archived webinar and learn about genome editing in hard-to-transfect cells
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Find out how to easily generate induced pluripotent stem cells from PBMCs or other cells using the Nucleofector™ Technology.
NYHED! NEB® Golden Gate Assembly Mix Did you know that NEB’s restriction enzymes and ligases can be used for Golden Gate Assembly, a technique for the efficient and seamless assembly of DNA fragments? Our new Golden Gate Assembly Mix incorporates digestion with BsaI and ligation with T4 DNA Ligase into a single reaction, and can be used to assemble up to ten fragments in a single step. Læs mere om Golden Gate Workflow
NYHED! Rapid PCR Cleanup Enzyme Set NEB Rapid PCR Cleanup Enzyme Set consists of two recombinant enzymes, Exonuclease I (Exo I, #M0293) and Shrimp Alkaline Phosphatase (rSAP, #M0371). It allows for rapid and complete enzymatic degradation of residual PCR primers and dephosphorylation of dNTPs subsequent to PCR. The Rapid PCR Cleanup Enzyme Set enables direct downstream applications including sequencing, genotyping, cloning or SNP analysis without the need for additional purification steps. The Rapid PCR Cleanup Enzyme Set is added directly to the PCR product after thermal cycling and is 100% compatible with commonly used PCR reaction buffers.
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NYHED! Antibodies for Cancer Research: PD-L1, PD-1, OX40, OX-40L, CTLA-4, CD40L validated for Flow and IHC • High Sensitivity: detect endogenous levels of target protein expression in human tissues or primary human cells • Target Specificity: no cross-reactivity and tested in relevant
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PBMC models • Monoclonal Antibodies: ensure lot-to-lot consistency • Optimized Protocol: provided for key applications • Custom Formulations available: for labeling and platform specific applications Læs mere her
CD40 Ligand (D5J9Y) Rabbit mAb #15094
PD-L1 (E1L3N®) XP® Rabbit mAb #13684
• CD40 ligand is expressed on T cells and activates signaling cascades such as MAPK and NF-κB when bound to CD40 • WB, IP, and IHC-P validated
• PD-L1 (B7-H1, CD274) inhibits activated T cells by binding to its receptor, PD-1, on the surface of activated T cells. • IHC, flow, WB, IP and IF validated
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Se flere PD-L1 mAb varianter
PD-1 (D3O4S) Rabbit mAb & Conjugates • PD-1, expressed on T cells, regulates immune tolerance by suppressing T cell signaling when bound to PD-L1 or PD-L2 • Flow cytometry validated Se PD-1 mAb varianterne her
NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
NEBNext® Ultra™ RNA Library Prep Kit for Illumina®
When determining which NEBNext product to
NEBNext Ultra kits are available for directional (strand-specific) or non-directional library
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choose, you’ll need to decide whether to use the Ultra or Standard workflows. Ultra workflows are faster, have fewer components and clean-up steps, and are optimized for lower input amounts. For DNA, the Ultra workflow library preparation is complete in 2.5 – 3 hours, with only 15 minutes of hands-on time. The Ultra workflow is optimized for input amounts between 5 ng and 1 µg and is also ideal for ChIP DNA. When time is of the essence and starting material is precious, Ultra kits ensure optimal yields.
preparation. These kits utilize streamlined workflows and novel master mixes, and have been designed for performance with input amounts as low as 10 ng (purified mRNA or rRNA – depleted RNA) for Directional RNA and non-directional RNA. Standard non-directional workflows are enabled by our standard kits, which support input amounts above 50ng. Our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.
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SE HER! Bestil det nye NEB 2015/16 katalog hos BioNordika og få et minutur, en floatie, en nøglering og en blyant fra NEB! Eller bestil på mail
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BioNordika Denmark A/S - Marielundvej 48, 1. - 2730 Herlev - 3956 2000 -
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