Transcript
Leica DMI 6000 B When Smart Becomes Simple: Brilliant Imaging At Your Fingertip! The Standard in “Intelligent” Inverted DigitalMicroscopy
Intelligence Brilliance Integration Flexibility
When Smart Becomes Simple Leica Microsystems presents the Leica DMI 6000 B Inverted Research Microscope, a system developed to help present your research in the best possible light. Optical excellence meets superior versatility to allow you to experience the pleasure of your work. The best possible image quality and intelligent operation – using a microscope has never been this easy, and you can depend on the benefits of a custom-tailored, application-specific microscope system with the Leica DMI 6000 B. Intelligent imaging – Simply press a button Enjoy the intelligence of the Leica DMI 6000 B, the microscope that makes it easy to capture outstanding images. With its Contrast, Illumination, and Stability Manager, the DMI 6000 B provides razor-sharp, brilliant images at the press of a button. Experience and innovation – The art of creating brilliant images Experience Leica Microsystems new fluorescence axis that offers everything that you could expect from a light microscope in terms of brilliance, depth of field, and resolution. The Leica DMI 6000 B also features an unparalleled innovation – The ultra fast IFW Integrated Filter Wheel.
Photo courtesy of ZFIN http://zfin.org
Powerful integration – Team players Leica Microsystems products are team players that are carefully attuned to one another and reinforce each other’s strengths. The Leica line ranges from microscopes and digital cameras to software solutions for a wide variety of applications. Assemble a custom system from Leica Microsystems line of products – and rest assured that everything will interact seamlessly.
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Adapted but individual – Flexibility Your research and requirements are unique and the microscope you use should be capable of seamless integration. Leica cooperates closely with leading manufacturers of microscope accessories to ensure that the Leica DMI 6000 B will reliably integrate into your system. We thank the IGBMC, Strasbourg, France, for their kind support in providing several application images.
Leica Design by Christophe Apothéloz
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Dr. M. Faretta, Dr. M. Garrè; IFOM-IEO Campus, Milano, Italy
“The term ‘intelligent automation’ doesn’t refer to the simple motorization of individual microscope functions. For Leica Microsystems, it means the smooth, well thought out automation of all functions. For users, it means they can focus on their actual work rather than on operating the microscope.” Oliver Jagemann, DMI Microscope Project Leader, Leica Microsystems
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Intelligence
Intelligent Imaging – Simply Press a Button Let there be light Change the objective – and adjust the brightness and diaphragms. How many times a day do you do that? As of now, it’s history! Leica Microsystems Illumination Manager handles it reliably. When changing objectives or contrast techniques, the Illumination Manager starts by automatically setting the brightness, aperture, and field diaphragms. Each time you tweak and optimize their settings, the microscope will adopt them immediately, which allows you to work quickly without constant readjustment. Bright white A better way to color balance: Don’t! Leica Microsystems integrated CCIC (Constant Color Intensity Control) maintains a constant color temperature of 3200 K, which compensates for changes in color temperature with lamp voltage that would normally require insertion of daylight filters or white balancing.
Illumination Manager Leica Microsystems Illumination Manager for custom diaphragm and light settings is located on the lefthand side of the microscope. Switching between transmitted light and fluorescence axes is simply a matter of pressing a button. Each change is automatically stored and displayed.
Less for more For longer cell viability, less is often more. Fluorescence excitation levels can be carefully selected using the FIM (Fluorescence Intensity Manager). Five levels of fluorescence intensity can be selected – and remembered for each filter cube.
Fluorescence Intensity Manager (FIM) The FIM disk is located in the aperture diaphragm plane of the fluorescence axis. It features stop plate disks of varying transmissivity for improved homogeneity and excitation brightness control. The regulation takes place in five fixed steps: 100%, 55%, 30%, 17% and 10%.
Color-neutral brightness control The CCIC module, located in the transmitted light axis, consists of a glass disk with a blue gradient filter. As the lamp voltage changes, the disk rotates to a new filter position, compensating for the change in lamp color temperature to maintain a constant 3200 K.
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Dr. W. Wittke, Leica Microsystems, Wetzlar, Germany
“The observation of living samples involves making unstained specimens visible under the microscope. Leica has made the contrast methods required for live cell imaging reliable and easy to apply. A touch of a button is all that’s needed. The proper contrast method – with the correct optical elements and the optimal diaphragm and light values – is set automatically. As a result, users always have the correct contrast for a specific specimen.” Stefan Motyka, Product Manager, Leica Microsystems
“The long-term observation of living samples is an important tool for researchers exploring processes in cells. To ensure the usability and comparability of their results, Leica has developed the Stability Manager, with functions that reduce thermal expansion of the instrument to a minimum and compensate for its effects automatically where required. Consistently razor-sharp images are the result.” Bernard Kleine, DMI 6000 B Project Manager, Leica Microsystems
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Intelligence
Intelligent Imaging – Simply Press a Button Contrast – as if by magic The days of tinkering around with condensers are over – and they won’t be missed! Simply press the “PH” button on the Leica DMI 6000 B, and the phase contrast is set automatically. The microscope knows which phase ring is needed for the objective currently in use and automatically positions it into the beam path. The operation of the Differential Interference Contrast (DIC) is even more astonishing: Just press a button and the analyzer, polarizer, and correct prism pair for the objective move into the beam path. Changing contrast techniques is a simple press of a button whether in transmitted light or fluorescence. Like a rock Microscope stands are made of metal. When it warms up, metal expands – that is a law of physics that can’t be circumvented. While we can’t change the laws of physics, Leica ensures that this does not interfere with your work. Long-term measurements can take hours or even days, and it’s important that the selected focal plane remains in the same place at the end of the experiment. The Leica DMI 6000 B is equipped with a temperature drift compensation, Stability Manager, which automatically offsets housing drift in the x, y, and z axes.
Leica’s new generation of condensers Leica’s S28 condenser (above) is fully motorized and embodies the Intelligent Automation concept of the Leica DMI 6000 B. Equipped with a motorized aperture and front lens, 7-position condenser turret, and separate polarizer, it reliably supports the functions of the Contrast Manager. All condensers of this series can be used for magnifications from 1.25x to 100x and attach directly to the illumination arm without cables. In addition, a vertical stop ensures the optimal position of the condenser.
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Long-term Measurements A microscope must fulfill a number of preconditions for use in long-term measurements. The system shown below is an integrated solution consisting of a Leica DMI 6000 B equipped with a DFC350 FX camera, FW4000 TZ fluorescence software, and accessories for the monitoring of optimal experimental conditions.
Leica DMI 6000 B:
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The Stability Manager ensures that all stage and focus parameters remain constant for the duration of the experiment. The Parfocality Manager provides a sharp image at all times – even after changing the magnification. The automated fluorescence axis supports full remote control. Together with the integrated shutter, Leica’s IFW ensures fast wavelength changes when using fluorescence methods. Bulky cameras can be connected to the bottom port.
Leica DFC350 FX:
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The camera features a FireWire port for fast image transfers. The cooled monochrome chip provides highest resolutions and noise reduction when using fluorescence methods.
Leica FW4000 TZ:
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K. Langenberg and Prof. Dr. P. Verrijzer, Erasmus MC Biochemistry, Rotterdam
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This software features driver solutions for cameras and microscopes, allowing remote control of the entire system. The Z-stacking and time-lapse modules have been developed specifically for long-term observation.
Intelligence
Intelligent Imaging – Simply Press a Button Freedom to do things your way The Leica DMI 6000 B features eleven freely programmable buttons for the functions most important to the user. Use them to create your own microscope – put the functions you need wherever you want them. Once in focus – always in focus Functional highlights of the Leica DMI 6000 B include the motorized z focus and its parfocality function. Objective sets have been designed to ensure that the focal planes of the individual objectives always lie in the same z plane. Minor deviations may arise due to production tolerances however, and as a result, it is necessary to correct the focus after changing objectives. The parfocality function compensates for different focal planes. In addition, the focal plane and a lower plane can be stored and selected automatically.
Free programming Four of the freely programmable buttons are located on the left side of the microscope. Seven additional buttons are on the right side of the instrument and on Leica’s SmartMove remote control module. Each of these buttons can be programmed without restrictions for a microscope that is perfectly configured to suit user requirements.
A clear overview The graphical display has been structured so that the user can see an overview with a quick glance. It cannot be obscured, even when using large climate chambers. The current status of the Leica DMI 6000 B is visibly displayed at all times.
Leica’s Focus Manager The Focus Manager, which records, manages, and travels to two focal planes, is located on the righthand side of the microscope. In addition to storing the focal plane, another lower level can be stored to ensure reliable objective changing, even with complex stage setups.
Status display The full status of the microscope’s current settings is visible at a glance. The current contrast method, magnification, illumination parameters, camera output port, and focal plane position information is provided.
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“With regard to optical performance, the Leica DMI 6000 B sets completely new standards that not only apply to the enhanced DIC. The fluorescence is even more brilliant than before due to new developments such as Leica’s light trap, Excitation Manager, and Integrated Filter Wheel.”
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G. Burger, Leica Microsystems, Mannheim, Germany
Peter Euteneuer, Manager, Optical System Planning, Leica Microsystems
Brilliance
Experience and Innovation – The Art of Creating Brilliant Images Contrast and high-resolution for every specimen – Leica’s new DIC Differential Interference Contrast (DIC) provides a relief-like impression of specimens in polarized light using two Wollaston prisms. An inverse relationship exists between contrast and resolution when working with contrast techniques such as DIC; better contrast results in a lower resolution and vice versa. This effect is more pronounced when observing specimens that are unusually thick or thin. Leica offers special prism combinations for such cases: Prism C for regular thickness, C1 for especially thick specimens, and C2 for especially thin specimens. A special highlight: The DIC bias adjustment is also intelligent. The microscope remembers your preferred bias setting and automatically restores the setting for each objective. It’s the fastest way to get stellar DIC images.
C. elegans recorded with DIC and Wollaston prisms with different splitting angles. Imagesource: IGBMC Strasbourg (F)
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Experience and Innovation – The Art of Creating Brilliant Images
A disk like no other is hidden in the heart of the fluorescence axis. Leica FIM (Fluorescence Intensity Manager), 2 fast shutters, a motorized Excitation Manager, and the IFW (Integrated Filter Wheel) are on a disk with a diameter of 49.5 mm.
Drosophila eye – FITC, CY3, DAPI, BGR
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Brilliance
Black as night A fast shutter is essential to reduce bleaching and fading during fluorescence observations. The Leica DMI 6000 B has an optimized internal shutter to ensure that light stops reaching the specimen in less that 100 milliseconds. Colorful as the rainbow Using multiple fluorescence labels within the cell is now commonplace for co-localizing events. In the past, this often required expensive external devices like fast-switching monochrometers to switch excitation wavelengths for each probe species. Now, the fast IFW in combination with a multi-bandpass emission filter cube allows nearly all GFP variants to be excited within 50 milliseconds. Balance is the key – the Leica Excitation Manager Leica’s Excitation Manager, integrated in the Leica DMI 6000 B, allows users to balance the brightness of specimens with multiple excitations directly at the microscope. The Excitation Manager is motorized and features sixteen levels to reduce glare from red or green excitations. The settings can be stored and reproduced whenever they are needed. Perfectly black background A blacker background leads to more brilliant fluorescence – and that’s why Leica developed the light trap. Multi-band filter cubes contain black glasses with highly polished, low-reflection surfaces that absorb undesirable stray light for a perfectly black background. Standing firm – Leica’s unique Zero Pixel Shift technology Only Leica filter cubes feature Zero Pixel Shift technology, which prevents the shifting of images when observing specimens with multiple excitations using different filter cubes. The user can superimpose images with confidence – a pixel from an FITC image will be at precisely the same spot in a DAPI image. Timeconsuming overlay matching is not required.
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Fluorescence Observation Fluorescence observation is a standard application for the study of living cells. The system shown below shows a system consisting of a Leica DMI 6000 B, a DFC350 FX camera and FW4000 I fluorescence software. Systems of this type are ideal for recording, archiving, and processing fluorescence images.
Leica DMI 6000 B:
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The automated fluorescence axis supports full remote control.
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The IFW changes excitation wavelengths in milliseconds.
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Zero Pixel Shift technology ensures perfect image alignment of multiple excitations, and makes software overlay compensation superfluous.
The 6-position filter cube changer offers enough space for a variety of fluorescence configurations.
The integrated shutter reliably protects specimens against fading. The Excitation Manager coordinates the intensity of multiple excitations. The Fluorescence Intensity Manager FIM regulates the excitation light to effectively protect specimens.
Leica DFC350 FX:
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The camera features a FireWire port for fast image transfers. The cooled monochrome chip provides highest resolutions and noise reduction when using fluorescence methods.
Leica FW4000 I:
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This software solution features driver solutions for cameras and microscopes, which allows remote control of the entire system.
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The Imaging application module has been specifically designed for the image processing sector.
Brilliance
Leica Microsystems new fluorescence axis How can you improve something that’s already outstanding? By studying the smallest details. Every single optical component of the Leica DMI 6000 B’s fluorescence axis was studied and optimized with regard to transmission, image flattening, and light flux qualities. The result is a fluorescence axis of peerless optical quality. Enough room for everyone’s favorite A filter turret for up to six fluorescence filter cubes – more than required for many examinations – is standard equipment on the Leica DMI 6000 B. And if an extra filter cube is ever needed, replacement is easy. Simply press a button to swing out the filter turret and replace the filter cube with a click. And, the filter disk positions the filter cubes into the beam path in less than 0.2 second.
Fast filter replacement Shown above is the open drawer of the 6-position filter holder for fluorescence filter cubes. The cover opens at the press of a button and automatically moves the filter cube in the beam path to the opening. Filter cubes can be replaced in seconds.
Everything at a glance The most important fluorescence functions – filter cube changeover and shutter function – can be monitored at a glance and controlled at the front panel. The display provides immediate feedback.
Clearly designed controls The most important fluorescence functions are grouped on the front panel. Each filter cube can be controlled directly. The shutter opens or closes at the press of a button. The display provides immediate feedback.
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“In the service department of the IGBMC, nearly twenty users per day use our microscopes. We buy Leica products because Leica Microsystems is our one stop source for cameras, software applications, and microscopes that complement one another perfectly. And every one of our systems can be configured to suit different researchers with different backgrounds who are investigating different topics. The multi-user software package that stores a variety of user profiles is helpful to us in this respect.” Dr. Jean-Luc Vonesch, Computer Scientist, Head of the Optical Imaging Unit and Confocal Microscopy, IGBMC Strasbourg
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Integration
Powerful Integration – Team Players A perfect fit for all users Microscopes are frequently shared by a number of users. Each user has different personal requirements. With Leica Microsystems MUP (Multi User Package) software, custom microscope configurations can be created and stored for each user. Microscope configurations can be restored by entering a personal password. Focus with the press of a button Brand new: Leica Microsystems autofocus software module for digital cameras. When using a Leica DFC280, DFC320 or DFC480 digital camera, the user benefits from an autofocus program that automatically finds and holds the focal plane. Digital cameras for any application Leica digital cameras feature standard FireWire ports for fast image transfers to PCs and Macintosh computers. The product range covers everything from color cameras for a variety of applications to monochrome cameras with cooling systems for highresolution fluorescence images. All Leica Microsystems digital cameras feature variable resolution and live-image mode, with resolutions ranging from 1.3 to 12 megapixels and 14-bit color depth per color channel. Perfect image archiving and analysis Leica Microsystems FW Fluorescence Workstation is a comprehensive solution for fluorescence applications. Depending on the requirements, users can choose between the Leica FW4000 I (Imaging) package for simple imaging, and the Leica FW4000 TZ package, which is designed specifically for time lapse and z-stacking applications. With Leica IM500 or IM1000 Image Manager software, Leica provides perfect image archiving functionality, with network support in the IM1000 version. Leica QWin software was designed for special requirements, and allows users to program their own work routines in a macro editor.
Leica DFC320 digital camera
Leica FW4000 image archiving
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Imaging System for the Observation of Living Cells Imaging systems are fundamental to research departments requiring the recording, processing, and archiving of images. Ease of use and suitability for universal deployment are required. Shown here is a system consisting of the Leica DMI 6000 B, DFC480 camera, IM1000 archiving software, and the QWin application. Leica DMI 6000 B:
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The Contrast Manager allows fast changes between contrast methods – at the press of a button. The Illumination Manager always delivers optimal lighting – regardless of the magnification or contrast method. The Parfocality Manager ensures a sharp image at all times – even after changing the magnification. The automated fluorescence and transmitted light axes support full remote control. The 6-position fluorescence filter changer provides enough room for multiple users. Remote control via SmartMove ensures a lack of vibration during the experiment. Four camera ports permit the deployment of a variety of cameras.
Leica DFC480
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The camera features a FireWire port for fast image transfers. The color chip is suitable for both fluorescence and transmitted light applications.
Leica IM1000
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This software features driver solutions for cameras and microscopes to allow remote control of the entire system. All recorded images are immediately archived. The Store and Recall module allows microscope and camera settings to be reproduced at any time.
Leica QWin
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Custom workflows can be programmed as required with the macro editor.
Integration
Powerful Integration – Team Players Many camera port configurations to choose from One camera for fast live images, one camera for high-resolution fluorescence images, and one for video. While that is not unusual, it frequently poses problems for users. The Leica DMI 6000 B features four camera ports. The motorized base port is ideal for bulky cameras. Two lateral motorized ports (left and right) round out the selection. Another mechanical port on the tube was developed for highly accessorized systems with no side room. A special highlight of Leica Microsystems camera port program: Choose which ports to use and how much light they will receive. A product portfolio of thirteen different components – prisms with varying degrees of transmissivity and even color splitters – can be combined for more than 200 different camera port configurations – enough to find the ideal solution for your requirements. Repetition required An experiment is to be repeated weeks or even months later with new specimens. The images of the different specimens are to be compared. Until now, taking exactly comparable images was virtually impossible. Not so with Leica’s new Store and Recall module! This software module stores the complete settings of the microscope together with the image. To take a new image under the same conditions, pressing a single button will restore the old settings – including the light settings for fluorescence and fine adjustment of the DIC!
Motorized camera ports The two lateral ports are controlled by a motorized disk with four positions that can be equipped to suit individual requirements. The disk can be used for beam splitters to divert 100%, 80%, or 50% of the light to the left or right port. It can also accommodate a beam splitter with wavelength splitting. The base port is controlled by a motorized slide that diverts 100% of the arriving light to the port.
Mechanical camera port The upper camera port in the tube is mechanical and can be equipped with two (without Bertrand lens) or three (with Bertrand lens) switch positions.
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“Providing the best possible conditions for organisms is essential when observing living cells with a microscope. Users can count on Leica Microsystems for the correct accessories for any application, and ambient conditions for cells can be monitored at all times during
Dr. Katja Peter, Marketing Manager, Research Microscopes, Leica Microsystems
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Dr. M. Hurtado, Swiss Federation for Artificial Insemination, Mülligen, Switzerland
an experiment.”
Flexibility
Temperature to fit the experiment Stage inserts for all types of containers, combined with temperature control units, ensure correct temperatures for your experiments. From 3°C to 60°C, anything is possible. Simply choose the components that best suit the experiment’s requirements. Everything under control A comprehensive range of control equipment is available for the Leica DMI 6000 B. Carbon dioxide control units maintain constant pH values. Oxygen controllers regulate the O2 concentration required by the cells. Temperature regulators are also available that are capable of maintaining any required physiological temperature.
Heating and cooling inserts Leica’s wide range of heating and cooling inserts covers the needs of virtually any user. Inserts for Petri dishes, coverslips, multi-well dishes, and other containers can be integrated in the stage of the Leica DMI 6000 B. An M24 multi-well dish-heating insert is shown above.
Perfect climate Specimens require defined environmental conditions for experiments. Leica Microsystems offers climate chambers in a variety of sizes – from models designed to accommodate a single Petri dish, to one that encloses the entire microscope.
Controllers The CTI Controller 3700 (above) is used for the control of temperatures and carbon dioxide. Temperatures between 3°C and 60°C can be specified.
Incubators Shown above is the Incubator S, which was designed for use with Petri dishes and glass slides. The transparent incubator housing is placed on a heating insert or heating stage. Glass inserts are integrated in the covers for observation in DIC contrast.
For detailed descriptions of the various stages, heating and cooling inserts and control instruments, please see Leica’s separate brochure, “Live on Stage”. Order number: 914 352
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Micromanipulation Micromanipulation places high demands on microscope systems. The system shown below is an integrated solution consisting of the Leica DMI 6000 B equipped with a DFC320 camera, IM1000 archiving software, and accessories for monitoring experimental conditions. Leica DMI 6000 B:
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The Contrast Manager provides fast, low-vibration changes between a variety of contrast methods. The Parfocality Manager ensures a sharp image at all times – even after changing the magnification. The automated fluorescence axis supports full remote control. The micromanipulation stage with its slim design allows easy adaptation of manipulators. The heating insert ensures optimal experimental conditions for the cells. Remote control via SmartMove ensures a lack of vibration during the experiment.
Leica DFC320
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The camera features a FireWire port for fast image transfers.
Leica IM1000
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This software features driver solutions for cameras and microscopes and allows remote control of the entire system.
Leica Micromanipulator
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The mechanical manipulator transmits power directly and without delay.
Flexibility
Like an extra hand Cell manipulation belongs to the standard science of biomedical researchers. The mechanical Leica micromanipulator is unparalleled in precision and quality, and it has been optimally adapted to the Leica DMI 6000 B. Suitable adapters for electrical and hydraulic manipulators are also available. For tasks such as these, the mechanical Leica micromanipulator is unparalleled in its precision and quality, and it has been optimally adapted to the Leica DMI 6000 B. Leica has also realized suitable adapters for electrical and hydraulic manipulators for users preferring such instruments.
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b
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d Mouse egg chromosome removal sequence (UV and transmitted light): a before removal; b insertion of pipette; c removal; d verification of removal Image source: IGBMC Strasbourg (F)
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Stand
Focus
Power Supply
Leica DMI 6000 B • In CTR6000 electronics box
Display
• Information display 77 x 49 cm (W/H)
Interfaces
• RS232 • Ready for USB
General
• Motorized • 5 programmable stepsizes • Switching between coarse and fine mode • 6x M25 • Motorized, absolute coding
Objective Turret Stand
• 7 freely programmable buttons • Buttons with fixed functions for – Illumination manager, focus functions, camera ports, subsequent magnification, fluorescence functions
SmartMove (Remote Control)
• Control element for focus (z) and stage movement (x, y) • 4 freely programmable buttons
Mechanical Stages
• Fixed stages – Ceramic-coated – Over 20 different inserts available – Can be equipped with heating and cooling inserts
Motorized Stages
• 3-plate cross-stages (also slim form for micromanipulator) • Spindle stage
Transmitted Light Axes
Illumination Arm
• 12 V/100 W halogen lamp • Motorized field diaphragm (mechanical optional) • Filter magazine for 2 filters, motorized (mechanical optional) • Motorized shutter (mechanical optional)
Condensers
General
• Condenser turret for optical elements with – 4 large openings for prisms, DF stop, BF, PH rings – 3 small openings for BF, PH rings • Integrated motorized aperture diaphragm (mechanical optional) • Suitable for magnifications from 1.25x to 100x • Separate polarizer (optional; mechanical or motorized)
S1–28
• Motorized condenser turret (coded optional) • Motorized condenser head (mechanical optional)
S70
• Motorized condenser turret (coded optional) • Fixed condenser head • Motorized lens for low magnifications
Filter Disk
• Motorized up to 6 filter cubes
Illumination
• 100 W halogen lamp
Focus
• Including parfocality function • Positioning memory for fast traversal to 2 z-positions
Illumination Manager, Transmitted Light
• Adjustment of brightness, aperture and field diaphragms to the objective and contrast technique currently in use
CCIC
• Color-neutral brightness control – Maintains color temperature of 3200 K
Contrast Manager
• Adaptation of optical elements such as prisms and light rings to the objective and contrast technique currently in use
Illumination Manager, Fluorescence
• FIM (Fluorescence Intensity Manager) – Adaptation of brightness in 5 fixed levels • Adjustment of field diaphragm to the eyepieces or camera chips (round or rectangular diaphragms) • IFW (Integrated Fast Filterwheel) – Very fast switching of excitation with changeover times of less than 0.05 second • Leica Excitation Manager – Red-green attenuation in 8 levels
Controls
Stages
Fluorescence Axis
Automation
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265 mm
Specifications
126 mm 151 mm
410–510 mm
665 mm
268 mm
623 mm
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TV systems
System Overview Leica DMI 6000 B
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1.25x
1x
0,5x
11 541 517
11 541 510
11 541 537
11 541 511
11 541 512
11 541 538
Digital cameras, see Special brochures
Leica DC150 Leica DC180 Leica DFC280
Monitor
TV camera without optics
Leica D(F)C300 (F/X) Leica DFC320
11 541 518
C-mount HC
C-mount adapter HC 0.5x
0,5–2,4x ENG(B) mount HC
1.25x
11 541 541 F-mount 2/3
0,35x
1x
11 541 540 F-mount 1/2
0,33–1,6x
1x
11 541 539 B-mount 2/3 Sony
1x
11 543 702 B-mount 1/2 Sony
0,63x
11 543 706 C-mount 1/2
MFK2
Leica D(F)C350 (F/X)
11 541 043 DM MFK 2 Video measurement system
Leica D(F)C480
Image analysis/ Image storage
Leica DC500
Condensers 1b
S1–28 mot.
S1–28 man.
S70 Filter Ø 32 mm
Filter Ø 32 mm
11 521 515 3x Filter holder Filter Ø 32 mm
11 522 010 Man. polarizer
11 521 515 3x Filter holder Filter Ø 32 mm
11 522 010 Man. polarizer
11 522 009 Mot. polarizer 11 522 004 Mot. condenser base S1–S28 with pivoting head
11 522 007 Mot. condenser base S1–S28 with fixed head
11 522 005 Man. condenser base S1–S28 with pivoting head
11 522 006 Man. condenser base S1–S28 with fixed head
11 522 024 Adjustment lens for low magnification 11 522 008 Man. condenser S70
Light ring set
Light ring set
Condenser prisms
11 521 002 Spacer ring
11 505 150 Condenser head S1/0.9 dry
11 521 500 Condenser head S23/0.52
3
11 505 175 Condenser head S28/0.55
2
11 888 277 without Bertrand lens
1
Condenser prisms
11 521 002 Spacer ring
11 551 004 Condenser head S1/1.4 oil
11 551 004 Condenser head S1/1.4 oil
11 505 150 Condenser head S1/0.9 dry
11 521 500 Condenser head S23/0.52
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12
1
9
3
Trino ErgoTubes 12 With trino ErgoTube 2
10
1
9
9
6
6
3
10
8 6
6
5
INT AP
7
FD
TL/L
5
11
12
FD
INT AP
7
TL/L
Base stand* 4
8
5
INT AP
7
4
11
12
* prepared for bino tube
11 888 289 without bottom port 11 888 288 with bottom port
FD
TL/L
Base stand* with IMC optics
11 888 291 without bottom port 11 888 290 with bottom port
5
INT AP
7
Base stand* 4
11
12
11 888 287 without bottom port 11 888 286 with bottom port
Base stand* with IMC optics 4
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* prepared for trino tube
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11 888 285 without bottom port 11 888 284 with bottom port * prepared for trino tube
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12
FD
TL/L
* prepared for bino tube
12
2
11 888 253 with Bertrand lens
11 888 275 without Bertrand lens
10
Condenser prisms
11 505 175 Condenser head S28/0.55
2
11 888 276 with Bertrand lens
1
9
Light ring set
Bino ErgoTubes With bino ErgoTube
10
(in prep.) Mot. condenser S70 complete
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Side ports 11
8
11 888 109 Supply unit
11 888 255 Motorized side port left only
11 888 259: Side port prism 100% left 11 888 260: Side port prism dichroic 630 nm left 11 888 262: Side port prism 80% left 11 888 264: Side port prism 50% left
11 888 272 Motorized side port right only
11 888 258: Side port prism 100% right 11 888 261: Side port prism 80% right 11 888 263: Side port prism 50% right
11 888 273 Motorized side port right and left
11 888 258: Side port prism 100% right 11 888 259: Side port prism 100% left 11 888 260: Side port prism dichroic 630 nm left 11 888 261: Side port prism 80% right 11 888 262: Side port prism 80% left 11 888 263: Side port prism 50% right 11 888 264: Side port prism 50% left
Micromanipulator M right/left
CTR6000
11 505 180 Remote control SmartMove
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11 522 040 Base plate for Leica DMI6000 B
Transmitted-light axes
Fluorescence lamp housings
1
5
Light filter Ø 40 mm + Stop mot.
Light filter Ø 40 mm man.
11 504 107 Motorized mirror housing for 3 inputs
11 504 106 Lamp housing 106z L 6 Lenses Hg 100 W 1 Inch collector
11 504 108 Manual mirror housing right/straight
1c
11 504 114 Lamp housing 106z 6 Lenses Hg 100 W 1 Inch collector
1c
CCIC
11 504 109 Manual mirror housing left/straight
1b 11 522 025 Mot. illumination carrier incl. CCIC, mot. field diaphragm, mot. shutter, mot. filter magazine
11 504 105 Lamp housing 106z L 6 Lenses Xe 100 W 1 Inch collector
11 504 110 Lamp adapter 90 right
1b 11 522 026 Man. illumination carrier incl. man. field diaphragm, man. shutter, man. filter magazine
11 500 325 Power supply Hg 100 W
11 500 324 Power supply Xe 75 W
11 504 111 Lamp adapter straight
11 504 112 Fiber-optics coupling 1 m
11 504 113 Fiber-optics coupling 2 m
1
Fluorescence axes and magnification changer Transmitted-light lamp housings
6
1c
11 888 300 6x fluor turret with ultrafast filter wheel, 1 x tube lens and magnification changer
11 504 101 Lamp housing LH107 L 12 V 100 W Halogen (2.5 m)
11 888 689 1.5x magnification 11 504 102 Lamp housing LH107 L 12 V 100 W Halogen (0.7 m)
Fluorescence filter system (for order numbers see module brochure)
Booster optics 7
11 522 027 Booster lens
11513900 Analyzer 11513898 POL/IG filter system
11 888 265 1.6x magnification
IMC systems 8 11 888 283 Front module and magnification changer 1.5x*
11 888 690 2.0x magnification 11 504 103 Lamp housing LH107/2 L 12 V 100 W Halogen (2.5 m)
11 888 266 6x fluor turret, 1 x tube lens and magnification changer
11 504 104 Lamp housing LH107/2 L 12 V 100 W Halogen (0.7 m)
11 888 689 1.5x magnification
11 504 059 Lamp housing LH106 12 V 100 W Halogen (2.5 m)
11 888 690 2.0x magnification
11 888 265 1.6x magnification
11 888 301 6x fluor turret with ultrafast filter wheel, 1 x tube lens
11 504 058 Lamp housing LH106 12 V 100 W Halogen (0.7 m)
Fluorescence filter system (for order numbers see module brochure)
11 888 280 Front module and magnification changer 1.6x*
11513900 Analyzer
11 522 002 IMC slider
11 888 282 Front module and magnification changer 2.0x*
11513898 POL/IG filter system
11 888 281 Front module Fluorescence filter system (for order numbers see module brochure)
11 522 003 PhaCo slider
* Not simultaneously with motorized magnification changer Available May 2005
11513900 Analyzer
DIC systems
11513898 POL/IG filter system
9
11 504 091 Lamp housing LH106z L 12 V 100 W Halogen (0.7 m)
11 888 267 6x fluor turret, 1 x tube lens
11 504 070 Lamp housing LH106z 12 V 100 W Halogen (0.7 m)
Fluorescence filter system (for order numbers see module brochure)
IL objective prisms
11513900 Analyzer
Analyzer
11 522 029 Man. DIC objective prism disk, objective prisms (A, C, C1, C2, D, D1, E)
11513898 POL/IG filter system 11 888 269 1 x tube lens and magnification changer
IL objective prisms
Analyzer
11 888 689 1.5x magnification
Eyepieces 2
11 522 001 Mot. DIC objective prism disk, objective prisms (A, C, C1, C2, D, D1, E)
11 888 265 1.6x magnification
11 505 070 Focusing telescope
11 888 690 2.0x magnification
– Eyepieces – Reticle graticules – Stage micrometer (for part numbers see module brochure)
11 888 268 1 x tube lens
Objectives
Stages
3 11 513 860 Immersion oil 20 ml
10
11 513 861 Immersion oil 10 ml low-fluorescence
11 522 011 Regular fixed stage plate
11 513 859 Immersion oil 250 ml
11 522 012 Fixed heating stage plate 11 522 013 Fixed cooling stage plate
11 522 014 Objective guide for special holder frame
Special holder frame
Objective series M25
11 522 015 Regular micromanipulator stage
Base plates
11 522 016 Heating micromanipulator stage 11 522 017 Cooling micromanipulator stage
4
11 522 018 Object guide for special holder frame
Special holder frame
11 522 031 Base plate 12 mm 11 522 036 Base plate 25 mm
11 522 019 Regular 3-plate stage 11 522 020 Micromanipulation 3-plate stage incl. inserts
Rectangular insert
11 522 021 Motorized microman. 3-plate stage incl. inserts
11 522 022 and 11 522 023 Scanning stages Rectangular insert 11 531 559 Manual rotating stage (without illustration)
27
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