Exer 3/6 Trea In - Columbus Instruments

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Serial Number:__________________ (Please refer to this number for service) Exer 3/6 Treadmill, Treadmill Controller and Treadmill Software Instruction Manual 0257-904L, 02/18/2015 Copyright Columbus Instruments 2015 Columbus Instruments 950 North Hague Avenue Columbus, Ohio 43204-2121 U.S.A. E-mail: [email protected] Phone: (614) 276-0861 Fax: (614) 276-0529 http://www.colinst.com Table of Contents Exer 3/6 Treadmill, Treadmill Controller and Treadmill Software Instruction Manual Section Page Table of Contents 1 1. Introduction 1.0 System Overview 1.1 System Specifications 1.1.0 Exer 3/6 Treadmill 1.1.1 Optional Electric Stimulus 1.1.2 Optional Downhill Running Adapter 1.1.3 Treadmill Controller 1.1.4 Optional Calibration Wheel 1.1.5 Minimum Computer Specifications 1.1.6 CI-Bus Hub Interface 5 5 6 6 6 6 6 7 7 7 2. Computer Setup 2.0 Treadmill Software Installation 2.1 CI-Bus Hub Driver Installation 9 9 11 3. Hardware Setup 3.0 Typical Item Inventory 3.1 Assembling the Exer 3/6 Treadmill 3.2 Downhill Running Adapter 3.3 Connecting the Components 3.4 Setting the Angle of Inclination or Declination 13 13 16 21 26 28 4. Stand-Alone General Exercising without the PC 4.0 Stand-Alone Initialization 4.1 User Menu Settings 4.2 Loading the Subjects 4.3 Controlling the Treadmill 4.3.0 Odometer Reset 4.3.1 Run 4.3.2 Accel 4.3.3 Stop 29 29 30 32 33 33 33 34 34 1 Table of Contents Exer 3/6 Treadmill, Treadmill Controller and Treadmill Software Instruction Manual Section Page 5. Performing a PC-Controlled Experiment 5.0 Treadmill Controller Setup 5.1 Treadmill Shock Detection Setup 5.2 Incline Controller Setup 5.3 Treadmill Software Initialization 5.3.0 No Devices Detected or Manually Configure the Port 5.4 Manual Experiment 5.4.0 Treadmill Settings 5.4.1 Shock Detect Settings 5.4.2 Incline Settings 5.4.3 Start Manual Experiment 5.5 Profile Mode Experiment 5.5.0 Treadmill Settings 5.5.1 Creating a Profile 5.5.2 Start Profile Mode Experiment 35 35 36 36 37 38 39 40 41 41 42 44 45 45 47 6. Treadmill Controller User Menu 6.0 Restore Defaults 6.1 Serial Number 6.2 Run-Time Meter 6.3 Run-Time Odometer 6.4 Unit ID 6.5 Autocal Speed 6.6 Acceleration Settings 6.7 Operating Mode 6.7.0 Normal 6.7.1 Time Limited 6.7.2 Distance Limited 6.8 Exit 49 50 50 50 50 50 51 52 52 54 55 56 56 2 Table of Contents Exer 3/6 Treadmill, Treadmill Controller and Treadmill Software Instruction Manual Section Page 7. Treadmill Software Descriptions 7.0 Experiment >> Run 7.0.0 Manual Tab 7.0.1 Profile Mode Tab 7.1 Experiment >> View 7.2 Tools >> Device Scan 7.2.0 Adding Devices to the CI-Bus 7.2.1 Removing Devices from the CI-Bus 7.3 Window 7.4 Help 7.4.0 About 57 57 58 59 59 60 61 61 62 62 62 8. Maintenance 8.0 Treadmill Belt Tracking 8.1 Treadmill Belt Tension 8.1 Cleaning 63 63 63 63 3 (intentionally left blank) 4 SECTION 1 INTRODUCTION 1.0 System Overview The Columbus Instruments Exer 3/6 Treadmill is an economical treadmill designed for general-purpose animal exercising. The overall running surface is divided into individual lanes by use of clear or opaque lane dividers. By default, the outermost lane dividers are clear to allow the user to view the animal in the outside lanes. All remaining dividers are opaque to provide isolation. The treadmill is capable of exercising from 1 to 3 rats or from 1 to 6 mice simultaneously. The running surface can be inclined up to 25° above the horizontal in 5° steps. An optional Downhill Running Adapter allows the running surface to be declined down to 15° below the horizontal in 5° steps. A safety platform at the end of the belt will rescue injured or slow moving animals. The floor of the platform can be replaced with a shock-grid to provide electric stimulus for exercise training. A clear one-piece lid and hinge cover all of the lanes. The lid and dividers are easily removed for cleaning. The use of a brushless motor provides very long life and eliminates the need for brush replacement or commutator refinishing. The Treadmill Controller, which functions as the control interface for the Exer 3/6 Treadmill, allows adjustment of the belt speed and optional electric stimulus. The belt speed is adjustable from 0.0 to 102.3 meters per minute (m/m). A toggle switch commands the belt to “RUN”, “STOP” or “ACCELERATE”. The exercise session can be set to run automatically for a fixed time duration or odometer distance, providing the exact same exercise regimen to multiple groups of animals, typical for day-to-day exercise in between testing sessions. A two-line LCD display reports the current speed setting, treadmill belt status, odometer distances or time and the acceleration settings. The Treadmill Controller can automatically calibrate the treadmill belt speed and odometer distance using the optional Calibration Wheel. The optional electric stimulus is a 200 millisecond (ms) pulse of electric current adjustable from 0.34 to 1.60 milliamperes (mA). The pulse is presented 1, 2 or 3 times per second (Hertz, Hz). Individual toggle switches associated with each lane on the Exer 3/6 treadmill controls the application of the electric stimulus to the shock grids. The Treadmill Software communicates with the Treadmill Controller to provide PC-controlled exercise sessions saved as experiments. All of the treadmill settings, less the shock intensity, can be set and continuously varied throughout the session. During an experiment, all of the data reported by all components of the system, are logged in user-defined intervals to an output *.CSV file. This common filetype can then be imported into various spreadsheet tabulation programs such as Excel. Summaries of the features are as follows: • • • • • • • • • • • • The Exer 3/6 Treadmill can exercise 1-3 rats or 1-6 mice simultaneously. Any combination of clear or opaque lane dividers can be used. +25° of inclination in 5°steps. Optional -15° of declination in 5° steps. Brushless motor ensures long life with no maintenance! Treadmill belt speed is adjustable from 0.0 to 102.3 meters/minute. Acceleration is adjustable from 0.0004 to 25.5 meters/minute/second. Automatically timed exercise sessions up to 4:15:59s in 1s steps. Automatic distance limited exercise sessions up to 9,999.0 meters in 1.0 meter steps. Optional automatic treadmill belt speed and odometer distance calibration using the calibration wheel. Optional Electrical Stimulus, 0.34 to 1.60mA, 200ms pulse, 1, 2 or 3 times per second. The Treadmill Software commands the Treadmill Controller, Treadmill Shock Detection and Automated Incline Controller all in one interface! 5 1.1 System Specifications 1.1.0 Exer 3/6 Treadmill Physical Dimensions: Physical Weight: Mouse Lane Dimensions: Mouse Platform: Rat Lane Dimensions: Rat Platform: Angle of Inclination: 1.1.1 83.8 x 50.8 x 50.8cm (33.0” x 20.0” x 20.0”). 25 kilograms (55 pounds). 5.7 x 41.3cm (2.3” x 16.3”). 5.7 x 10.7cm with 1.0cm brush (2.3” x 4.2” with 0.4” brush) 11.8 x 41.3cm (4.6” x 16.3”). 11.8 x 10.7cm with 1.0cm brush (4.6” x 4.2” with 0.4” brush) 0° - 25° above the horizontal in 5° steps. Optional Electric Stimulus (replaces platform) Mouse Grid Dimensions: 0.3 x 11.4cm rods, 1.0cm apart, 5 - 6 rods typical (0.1” x 4.5” rods, 0.4” apart, 5 - 6 rods typical). Rat Grid Dimensions: 0.3 x 11.4cm rods, 1.0cm apart, 11 rods typical (0.1” x 4.5” rods, 0.4” apart, 11 rods typical) Stimulus Voltage: 163V. Stimulus Current: 0.34 - 1.60mA. Stimulus Duration: 200mS. Stimulus Repetition Rate: 1, 2 or 3 Hz. Stimulus Control: 2-position toggle switch (on/off). 1.1.2 Optional Downhill Running Adapter (modifies treadmill feet) Adapter Dimensions: Adapter Weight: Adapter Effect: Angle of Declination: 1.1.3 25.4 x 49.5 x 27.7cm (10.0” x 19.5” x 10.9”). 1.0 kilograms (2.3 pounds). adds +30.0cm (+11.8”) to the overall height. 0° - 15° below the horizontal in 5° steps. Treadmill Controller Power Requirements: Physical Dimensions: Physical Weight: CI-Bus Connection: Cal. Wheel Connection: Motor Connection: Shocker Connection: Belt Speed Range: Acceleration Range: Time Limited Range: Distance Limited Range: 200 watts, 100, 115, or 230VAC, 50/60Hz (factory selected). 33.1 x 29.0 x 10.2cm (13.0” x 11.4” x 4.0”). 3.2 kilograms (7.0 pounds). 8-pin RJ45 Jacks (x2). 4-pin Amphenol Circular Female Jack. 6-pin Amphenol Circular Female Jack. 15-pin Female D-sub. 0.0 - 102.3 meters/minute. 0.0004 - 25.5 meters/minute/second 0:00:1 to 4:15:59s. 1.0 to 9,999.0 meters. 6 1.1.4 Optional Calibration Wheel Power Requirements: Physical Dimensions: Physical Weight: Connector: Wheel Diameter: Output: 1.1.5 5VDC @ 100mA. 20.3 x 4.6 x 4.3cm (8.0” x 1.8” x 1.7”). 0.2 kilograms (0.5 pounds). 4-pin Amphenol Circular Female Plug. 4.128cm (1.625”). 100 pulses per revolution = 0.130cm per pulse. Minimum Computer Specifications IBM compatible computer with Windows operating system. CD-ROM drive for software installation. 20 MB of free hard drive space for software installation. 1.1.6 CI-Bus Hub Interface Power Requirements: Physical Dimensions: Physical Weight: Computer Connection: CI-Bus Connections: 5VDC (from USB) @ 500mA. 13.5 x 11.7 x 6.35cm (5.3” x4.6” x 2.5”). 0.6 kilograms (1.4 pounds). USB 2.0 Type “B” Jack. 4 ports (A, B, C & D) x 8p8c (RJ-45) 120ohm terminated. 7 (intentionally left blank) 8 SECTION 2 COMPUTER SETUP 2.0 Treadmill Software Installation You must have administrator rights/privledges on the host computer for the software to install successfuly. The software is expected to be installed and run locally on the host computer’s hard drive and not from a remote mapped network resource. Follow these steps to install the Treadmill software:
Insert the Treadmill Software CD-ROM into the host computer. If the “Treadmill Software Setup” application does not automatically launch, then you must manually launch the installation program.

Select “Start >> Run...” from the desktop taskbar. A “Run” window will open. Click the Browse button. A “Browse” window will open.




Select the CD-ROM drive from the “Look in:” combo box. Click once to highlight “Setup.exe” from the list of files on the CD-ROM. Click the Open button. The “Browse” window will close and return focus back to the “Run” window. Click the OK button to begin the installation program. Windows may pause to ask to allow an unknown program to make changes to the computer. Click the Yes button to continue. Upon initialization, some install files will be processed and copied to the host computer. These files will be removed if setup is cancelled. Then, a blue “Treadmill Software Setup” backdrop and installation window are displayed. 9 2.0 Treadmill Software Installation (continued)
Click the OK button to continue.
Click the square button marked with a computer and floppy disks to continue.
Click the Continue button to begin the copy and installation procedure into the default Program Group. The install program will check for necessary disk space, and then begin to copy the program. It is recommend to keep any files on your system that are newer than the ones being copied. Upon completion, the program will update the Windows system and create program icons inside the group folder on the start menu.
Click the OK button to finish the install program. The “Treadmill Software Setup” screen will close and return focus back to the Windows desktop. 10 2.1 CI-Bus Hub Driver Installation Next, the USB communication drivers for the CI-Bus Hub interface will be installed. If it did not automatically launch, then you will have to manually start the installation.

Select “Start >> Run...” from the desktop taskbar. A “Run” window will open. Click the Browse button. A “Browse” window will open.




Select the CD-ROM drive from the “Look in:” combo box. Double-click the “Config” folder to open, and then click-to-highlight “ftdi.exe” from the list of files. Click the Open button. The “Browse” window will close and return focus back to the “Run” window. Click the OK button to begin the installation program. Windows may pause to ask to allow an unknown program to make changes to the computer. Click the Install button or the Yes button to continue.
Repeat the above procedure and now select “cibus.exe”. That completes the copy and installation of the Treadmill Software with communication drivers. 11 (intentionally left blank) 12 SECTION 3 HARDWARE SETUP 3.0 Typical Item Inventory A typical Treadmill system will contain the following items: • • • • • • • • • 1 x CI-Bus Hub Interface, 4-ports with CI-Bus terminators (orange/blue plugs). 1 x USB cable, 1.8m (6 foot), A-B. 1 x CAT5 network cable, 1.8m (6-foot), 8p8c (RJ-45). The Treadmill Controller with AC power cord. 1 x Amphenol motor cable 1.8m (6-foot), 6-pin 6-conductor circular plug-plug. 1 x D-Sub shock cable, 1.8m (6-foot), 15-pin 15-conductor male-male. 1 x Calibration Wheel. 1 x Instruction Manual. 1 x Treadmill Software CD-ROM. 13 3.0 Typical Item Inventory (continued) • The Exer 3/6 Treadmill (shown with optional shock grid). • • • • • • 1 x treadmill lid 1 x feces tray. 1 x package of lane dividers: 5 opaque and 2 clear dividers are shown for mice. 1 x inclination rod, 51cm (20-inch) with spring pin. 2 x thumbnuts for securing the treadmill lid. 1 x 7/16-inch open-end wrench for adjusting the tension of the treadmill belt. 14 3.0 Typical Item Inventory (continued) You may also need the following tools for assembling the system: • Phillips (cross-point) screwdriver, medium tip. • Common (flat-point) screwdriver, small tip. • 5/16-inch (7.5mm) wrench. The Treadmill system is easy to assemble provided that the components of the system are placed correctly. A working surface that averages 1.3m x 1.0 m (6-foot x 3-foot, width x depth) with access to the front and back is recommended. It will facilitate adjustments to the inclination rod as well as loading and unloading the animals. When possible, it is recommended to use an AC power strip to provide a common access to AC power for all components of the Treadmill system and the host computer. Most AC power strips incorporate a master switch that can switch on and off the AC power to the entire system and protect against electrostatic discharge. It also provides a beneficial common ground between all components to help prevent unintentional ground loops which may exists between the individual AC power outlets of a room. 15 3.1 Assembling the Exer 3/6 Treadmill The Exer 3/6 Treadmill is about 90% assembled.
Remove any protective plastic film that may cover pieces of the treadmill.
Note that various holes are positioned along the length of the Inclination Rod. Insert the spring pin into the hole that is most near to the end. This end of the Inclination Rod is the bottom. The other end is the top. 16 3.1 Assembling the Exer 3/6 Treadmill (continued)

Roll the Exer 3/6 Treadmill on its side. Then insert the top of the Inclination Rod through the hole in the front of the treadmill. (Now it is easier to remove the protective film from the bottom of the treadmill too).
Roll the Exer 3/6 Treadmill onto its feet. 17 3.1 Assembling the Exer 3/6 Treadmill (continued)
Remove any protective plastic film that may cover the lane dividers.
Insert one of the clear lane dividers into the set of slots on the far side of the treadmill.
Insert all of the opaque dividers into the slots in the middle of the treadmill. Leave one set of slots vacant between the 2 dividers for rats. Or populate all 5 slots for mice (as shown). Then insert the other clear lane divider into the set of slots on the near side of the treadmill
18 3.1 Assembling the Exer 3/6 Treadmill (continued)
Remove all protective plastic film that may cover the treadmill cover and lid.

Fold the lid onto the cover. Place the holes of the cover over the posts in the front of the treadmill and secure with the two #6-32 thumb-nuts. 19 3.1

Assembling the Exer 3/6 Treadmill (continued) Unfold the lid onto the treadmill and secure with the 2 clips. Place the Feces Tray underneath the treadmill below the platform and treadmill belt. This completes the assembly of the Exer 3/6 Treadmill. 20 3.2 Downhill Running Adapter If the Exer 3/6 Treadmill came with the Downhill running adapter, then the system should contain the following: • • • • •
1 x Right leg with rubber foot. 1 x Left leg with rubber foot. 2 x Braces for the legs. 9 x #6-32 x 3/8-inch screws. 9 x #6-32 hex nuts with nylon locking inserts. Remove any protective plastic film that may cover the pieces of the Downhill Running Adapter. 21 3.2 Downhill Running Adapter (continued)
Raise the front so the treadmill will rest entirely on the rear panel. Remove the Inclination Rod with spring pin as set aside.
Using the Phillips screwdriver and 5/16-inch wrench, remove both feet from the bottom of the treadmill.
Using one screw and nut, connect the two braces together in the middle. Do not tighten. The screw and nut should hold the braces closely together but allow them to move. 22 3.2


Downhill Running Adapter (continued) Using four screws with nuts, connect the legs and the braces together in the orientation as shown. Again, do not tighten. The screws and nuts should hold the pieces closely together but allow them to move. Now, apply pressure to force the rubber feet of the legs apart and tighten the two screws and nuts near the feet. This will hold the feet farthest apart for better stability. Position the assembled Downhill Adapter with the braces on the bottom next to the bottom of the treadmill as shown and secure with the remaining 4 screws and nuts as shown. Tighten completely. 23 3.2 Downhill Running Adapter (continued)
Reposition the spring pin from the hole at the bottom to the hole in the middle of the Inclination Rod.
Insert the Inclination Rod through the hole in the front of the treadmill. Then lower the front so the treadmill rests on the Downhill Adapter feet and Inclination Rod. 24 3.2
Downhill Running Adapter (continued) Tighten all remaining screws and nuts of the legs and braces. This completes the assembly of the Downhill Adapter. 25 3.3

Connecting the Components Place the CI-Bus Hub interface near the host computer (not shown). Using the USB cable, connect the interface to the host computer. The power indictor (upper left LED of port “B”) should be lit continuously. Remove the orange terminator from one of the ports and replace it with one end of the CAT5 network cable. DO NOT LOSE THE TERMINATOR! It must be installed in the open port of the last device on the CI-Bus.

Connect the other end of the CAT5 network cable to one of the “CI-Bus” ports of the Treadmill Controller. Install the orange terminator into the other “CI-Bus” port. 26 3.3 Connecting the Components (continued)


Connect one end of the 15-pin shock cable to the rear “Shocker Box” port of the Treadmill Controller. Connect the other end of the 15-pin shock cable to the 15-pin D-Sub jack on the Exer 3/6 Treadmill. Use the common screwdriver to secure the 15-pin connectors to the ports.


Connect one end of the motor cable to the “Motor” port of the Treadmill Controller. Connect the other end of the motor cable to the 6-pin Amphenol jack on the Exer 3/6 Treadmill. Connect the AC power cord to the rear panel AC power receptacle of the Treadmill Controller and insert the other end into an appropriate AC outlet. DO NOT switch on the Treadmill Controller at this time! Refer to SECTION 4.0 – Stand-Alone General Exercising without the Computer or SECTION 5.0 – Performing a PC-controlled Experiment for further instructions. 27 3.4 Setting the Angle of Inclination or Declination The angle of inclination is set by the location of the spring pin in holes of the Inclination Rod. The holes range from 0° to 25° in 5° steps. With the Downhill Running Adapter, the holes range from 0° to -15° in 5° steps.


Lift the front of the treadmill to release the pressure on the spring pin and remove it from the Inclination Rod. Now position the treadmill slightly above the new height of inclination and replace the spring pin into the corresponding hole in the Inclination Rod. Slowly lower the treadmill onto the spring pin. 28 SECTION 4 STAND-ALONE GENERAL EXERCISING WITHOUT THE PC The faculties of the Treadmill Controller allow the user to provide various execise regimens for the animals. The belt speed and exercise duration of the session can be directly controlled and timed by way of the front panel. Likewise, once setup, the controller can automatically limit the exercise session by the distance traveled or by session duration. Typically, the controller is setup for the desired exercise session. Then the subjects are loaded. The shock intensity will be set and applied to the grids. Then the treadmill belt will be set in motion. When the session is finished, the shock is switched off and the subjects are removed. 4.0 Stand-Alone Initialization Attention! Use the following procedure to prevent the application of an undesirable treadmill belt speed or high intensity shock upon the application of AC power.
Disable the shock stimulus for all lanes by setting toggle switches toward the rear of the treadmill.


Turn the “INTENSITY” knob counter-clockwise, all of the way, to zero. Set the “TREADMILL BELT” toggle switch to “STOP”. Now, switch on the Treadmill Controller. The power switch is above the AC power receptacle on the rear panel. The front panel display should report the current speed setting, an accumulated odometer distance of zero, and the acceleration settings. 29 4.1 User Menu Settings Access the User Menu of the Treadmill Controller with this procedure:
Set the “TREADMILL BELT” toggle switch to “STOP”.
Set the “SPEED” to “88.7” m/min
Set the “REP RATE” knob to “2Hz”.
Then press the “ODOMETER RESET” button. The screen will display “User Menu” and then position you in the menu at the option for automatic speed and distance odometer calibration: “AutoCal Speed”.
Using the “REP RATE” knob as an up/down selector, select the next lower option on the menu by moving the knob down to “1Hz”, then back to “2Hz”. The screen will display the current acceleration settings. Refer to section: “6.6 Acceleration Settings” for specific details. In brief, when the “TREADMILL BELT” toggle is set to “ACCEL”, then the belt speed will accelerate (or decelerate) from the current speed to the ending speed. 30 4.1
User Menu Settings (continued) Using the “REP RATE” knob, select the next lower menu option by moving the knob down to “1Hz”, then back to “2Hz”. The screen will display the current mode of operation. If you would like to exercise the animals for a fixed amount of time or distance, choose the appropriate mode: “Time Limit” or “Dist Limit”, else select “Normal” in which by hand, you must monitor the time or distance and manually terminate the session. Refer to section: “6.7 Operating Mode” for specific details.
Using the “REP RATE” knob, select the next lower menu option by moving the knob down to “1Hz”, then back to “2Hz”. The screen will display “Exit”. Press the “ODOMETER RESET” button to exit the user menu. 31 4.2 Loading the Subjects Before loading or unloading the subjects, place a paper towel on top of the treadmill cabinet to protect the toggle switches and indicators and not allow urine to fall inside. Turn the clips that secure the lid and fold the lid on top of the cover. Load the subjects onto the shock grids (which are off). Reclose the lid and secure with the clips.





Set the “REP RATE” to “1Hz” (the “INTENSITY” should already be zero). Enable the shock stimulus for each lane by setting each toggle switch toward the front of the treadmill. The stimulus indicator will begin to flash on the treadmill as well as the controller. Slowly increase the “INTENSITY” until all of the subjects move from the shock grids. Press the “ODOMETER RESET” button. In the Normal mode (indicated by “D”), the odometer is reset to zero. In the Time Limit mode (indicated by “T”), the odometer is replaced by a timer which is preloaded to the duration specified in the user menu. In the Dist Limit mode (indicated by “L”), the odometer is preloaded to the distance duration specified in the user menu. Then set the “TREADMILL BELT” mode to “RUN” or “ACCEL”. The mode indicator, in the middle of the display, will show a progress bar or ramp that grows from left to right. Likewise, the odometer will begin to count up or the duration values will begin to count down. Observe the subjects. If any begin to rest on the shock grids, you may increase the “REP RATE” to “2Hz”, or “3Hz” or increase the shock “INTENSITY” setting. 32 4.3 Controlling the Treadmill The following sections discuss various controls and their effect on the operation of the Treadmill Controller. 4.3.0 Odometer Reset In the Normal mode, the odometer will display the accumulated distance traveled. When the “ODOMETER RESET” is pressed, the odometer will be reset and held at zero. It will begin to count up when the button is released. In a Time Limit or Dist Limit mode, the remaining duration of the session time or distance is displayed. When the duration reaches zero, the treadmill belt will automatically stop, terminating the exercise session. Simply replace the animals with the next group and press the “ODOMETER RESET” button. The duration counter will be reloaded and automatically the treadmill belt will start and run again.
Press the black round button in the lower left corner on the front of the Treadmill Controller to reset the odometer or duration. It will begin to count up or down when the button is released. 4.3.1 Run When the “TREADMILL BELT” toggle switch is set to “RUN”, the treadmill belt is enabled to run and the speed can be continuously adjusted by the “SPEED” knob. The display will report the current speed as set by the “SPEED” knob and the mode indicator will be a progress bar that grows from left to right. The odometer or duration will begin to count up or down. 33 4.3.2 Accel When the “TREADMILL BELT” toggle switch is set to “ACCEL”, the treadmill belt is enabled to run and the speed will begin to accelerate (or decelerate) from the current speed as set by the “SPEED” knob) toward the end speed, in 0.1meters/minute steps, at the acceleration rate displayed on the screen. The display will report the current speed of the treadmill belt and the mode indicator will be a staircase that grows when accelerating or shrinks when decelerating. 4.3.3 Stop When the “TREADMILL BELT” toggle switch is set to “STOP”, the treadmill belt is disabled from running and the speed will rapidly decrease to zero. The display will report the speed set by the “SPEED” knob and the mode indicator will be blank. In “Time Limit” mode, the duration will also freeze to preserve the remaining time in the session. 34 SECTION 5 PERFORMING A PC-CONTROLLED EXPERIMENT The Treadmill Software provides more control over the treadmill than the Treadmill Controller alone. The belt speed and acceleration can be varied continuosly as well as the shock switched on and off throughout the entire experiment session. Of course, data from the treadmill system are collected and logged into the experiment data file. 5.0 Treadmill Controller Setup Attention! Use the following procedure to prevent the application of an undesirable treadmill belt speed or high intensity shock upon the application of AC power.
Disable the shock stimulus for all lanes by setting toggle switches toward the rear of the treadmill.


Turn the “SPEED” knob counter-clockwise, all of the way to the end-stop. Set the “TREADMILL BELT” toggle switch to “RUN”. Now, switch on the Treadmill Controller. The power switch is above the AC power receptacle on the rear panel. The front panel display should report the current speed as “0.0”, the mode indicator will be a progress bar that grows from left to right, the odometer distance as “0.0” and the current acceleration settings. 35 5.1
Treadmill Shock Detection Setup Switch on the Treadmill Shock Detection by fully inserting the DC Coaxial power plug into the power receptacle on the rear panel of the unit. The first option of the menu should be displayed “Mode: Manual”. If the unit already has power, you may press the “RESET” button to quit the current operation and return to the top of the menu (“Mode: Manual”). 5.2





Incline Controller Setup Turn the “RATE” knob counter-clockwise, all of the way to the end-stop. Set the mode toggle switch to “STOP”. Now, switch on the Incline Controller. The power switch is above the AC power receptacle on the rear panel. The front panel display should report the position of the incline knob (upper left), the incline of the apparatus (upper right), the minimum incline rate (0.1°/s, lower left) and the mode of “STOP”. Adjust the “INCLINE” knob so the desired position matches the apparatus’ incline position to ensure the apparatus will not move when enabled. Now set the mode toggle switch to “RUN”. Adjust the “INCLINE” knob to 0.0°. 36 5.3
Treadmill Software Initialization On the host computer, launch the Treadmill Software. A “Treadmill Software” window should open. Then a “Commuication Setup” window will open. The software will automatically check every available COM port for the software’s configured devices. When found, a “Communication Scan” window will replace the previous and the software will enumerate all configured devices and report their status. The window will hold for a few seconds then close and leave you at the main menu of the program. If no devices were found, then use the following procedure to troubleshoot the issue: 37 5.3.0 • No Devices Detected or Manually Configure the Port Check Window’s “Devices and Printers” for the “CI-Bus Hub”. If it does not exist perform the following procedure:
Exit the Treadmill Software program.
Disconnect the USB cable between the computer and the CI-Bus Hub.
Refer to section: “2.1 CI-Bus Hub Driver Installation” and install the drivers.
Reboot the computer if needed.
Connect the CI-Bus Hub to any available USB port on the computer and wait for Windows to recognize the device and install the drivers.
Open Window’s “Devices and Printers” and check for the “CI-Bus Hub”. If it doesn’t exist, try another USB port on the computer. If the CI-Bus Hub cannot be detected, contact Columbus Instruments for further assistance.
Once detected, close “Devices and Printers” and launch the Treadmill Software. • Check the CAT5 Networking cable connections
Remove, then fully insert the cables into the CI-Bus ports of the CI-Bus Hub and other devices.
Be sure there are four (4) orange terminators, one (1) for each port (A, B, C, D) of the CI-Bus Hub. Each terminator should be placed at the end of the CI-Bus in the available CI-Bus port of the last device.
Click the Scan Ports button to continue. • Check the AC power to all devices.
Switch “Off” all devices and wait one minute.
If connected, switch “On” the AC-power to the Treadmill Shock Detection Unit(s) first.
Then switch “On” all other remaining devices (Treadmill and Incline Controllers).
Check them for normal operation and that they respond to button-presses and control adjustments.
Click the Scan Ports button to continue. 38 5.4 Manual Experiment In a Manual Experiment, only one speed and acceleration are allowed for the session.
Select “Experiment >> Run” from the main menu. 39 5.4.0 Treadmill Settings
Enter the “Interval” which you would like to have data logged to the output file. The number must be an integer and is typically set to “1”.
Enter the “Duration” of the entire experiment session. The number must be an integer and is typically larger than the “Interval” (example: 10 minutes). A value of zero will allow the experiment to run indefinitely, which then must be manually stopped by the user.
Click-to-clear “Enable Profile Mode”.
Use the mouse to select the text boxes and enter the following parameters: • Acceleration Step (Accel Step): 0.0 to 25.5 meters per minute (m/m). • Acceleration Interval (Accel Int): 0 to 255 seconds (sec). • Ending Speed (Max Speed): 0.0 to 102.3 meters per minute (m/m). • Starting Speed (S_Speed): 0.0 to 102.3 meters per minute (m/m). For example, to begin at 5.0m/m and apply an acceleration equal to 1m/m every 2 minutes up to the ending speed of 20.0m/m, use the following settings: • Accel Step: 1.0 • Accel Int: 120 • Max Speed: 20.0 • S_Speed: 5.0
Disable the shock for the session by selecting “Off”. Else, enable the shock by selecting “1Hz”, “2Hz” or “3Hz”.
Copy the shock intensity setting from the front panel of the Treadmill Controller into the “Shock Intensity” drop-down box in the form. Note: The Treadmill Software CAN NOT adjust the shock intensity! The shock intensity is manually set by the front panel of the Treadmill Controller. 40 5.4.1 Shock Detect Settings When the Treadmill Shock Detection unit is detected, the following controls are available:
Use the mouse to select the text boxes and enter the following parameters: • Session Number: 1 to 10. Not needed. Included for consistency with other software. • Number of Active Channels: 1 to 8. Set for the number of animals, typically 3 or 6. • Number of Visits to Grid: 1 to 999. Limits the shock by the number of visits to the grid. • Number of Shocks: 1 to 999. Limits the shock by the total number of shocks received. Likewise, the following statuses for each channel are also provided: • • • Active: The shock is on. The grids are active and monitored for shocks received. Idle: The shock is off. A limit has been reached, either the number of visits or shocks. No Shock: The shock is off. Temporarily disabled for the current period in a profile mode experiment. 5.4.2 Incline Settings When the Incline Controller is detected, the capabilites of the apparatus are displayed and the following controls are available:
Use the mouse to select the text boxes and enter the following parameters: • Belt Angle: Limited by the “Range” as indicated above. • Inc_Rate: Non-editable. Set to the maximum rate of the incline apparatus. The rate of inclination (Inc_Rate) is set to the maximum the apparatus will allow to provided the highest speed and lowest transistion times between different angles. 41 5.4.3 Start Manual Experiment Click the Start button to begin the experiment. A “Save Experiment Data File” window will open.
Enter the name for the data file and click the Save button. The window will close and return to the experiment window. The hardware will initialize and display:
The shock has been electronically switched off. Now, load the animals into the treadmill and click the OK button. First, the shock will be enabled and the treadmill belt will accelerate from reset (0.0 meters/minute) to the Starting Speed at a default acceleration rate of 2.0 meters/minute/second. Once the Starting Speed has been reached, the acceleration rate will change as specified by the Acceleration Step and Acceleration Interval and now the belt speed will accelerate (or decelerate) toward the Ending Speed. The Treadmill Shock Detection unit will also begin to monitor and report data from the shock grids. The Incline Controller will move from 0.0° to the “Belt Angle” as specified. 42 5.4.3 Start Manual Experiment (continued) The Experiment window provides the following information while the experiment is running: • • • • Belt Status: “Idle” indicates the front panel switch is set to “STOP” “Running” indicates the front panel switch is set to “RUN” “Accel” indicates the front panel switch is set to “ACCEL” Spd(m/m): reports the current speed of the treadmill belt. Dist Tr (m): reports the total distance the treadmill belt has moved. Belt Angle: reports the current angle of inclination. The grid below the status area reports the data collected for each interval of the experiment. The first report, “Int: 0”, details the settings for each channel at the start of the experiment. The last interval is the data at the termination of the experiment, which typically coincides with the last interval. • Stop : used to manually terminate an experiment. Necessary when the experiment “Duration” has been set to zero. 43 5.5 Profile Mode Experiment In the Profile Mode, the speed, acceleration, shock and incline are all dynamically adjustable througout the session. 44 5.5.0 Treadmill Settings Note: Controls not specified in the following procedure are “not needed” as they are automatically updated and set by the profile.
Enter the “Interval” which you would like to have data logged to the output file. The number must be an integer and is typically set to “1”.
Click-to-clear “Enable Profile Mode”.
Disable the shock for the session by selecting “Off”. Else, enable the shock by selecting “1Hz”, “2Hz” or “3Hz”.
Copy the shock intensity setting from the front panel of the Treadmill Controller into the “Shock Intensity” drop-down box in the window. Note: The Treadmill Software CAN NOT adjust the shock intensity! The shock intensity is manually set by the front panel of the Treadmill Controller. When the Treadmill Shock Detection unit is detected, the following controls are available:
Use the mouse to select the text boxes and enter the following parameters: • Session Number: 1 to 10. Not needed. Included for consistency with other software. • Number of Active Channels: 1 to 8. Set for the number of animals, typically 3 of 6. • Number of Visits to Grid: 1 to 999. Limits the shock by the number of visits to the grid. • Number of Shocks: 1 to 999. Limits the shock by the total number of shocks received. 5.5.1 Creating a Profile
Click the “Profile Mode” tab at the top of the form. The form will now display the current profile as a list of periods (or steps) and graphically over time for a visual reference. A profile is developed by sequentially defining any number of periods. Each period defines a new ending speed and time interval. Along with the starting speed, (usually the previous period’s ending speed) the acceleration is calculated for that period. Also, the shock can be enabled (“True”) or disabled (“False”) for that period. When available, the incline of the belt can also be adjusted upon the start of each period. By default, the first period (or step), begins at rest (starting speed of 0.0 meters/minute). When additional periods are added, the previous “End Speed” is copied as the next period’s “Start Speed”. 45 5.5.1 Creating a Profile (continued)
Use the mouse to select the text boxes and enter the following parameters: • • • • •
End Speed: Period: Belt Angle: Shock: Comment: 0.0 to 102.3. 0 to 32767. -10.0 to +25.0. False or True. optional text The desired speed at the end of the period. The duration of the period in seconds. The desired incline for the entire period. Removes or applies the shock for the entire period. Describes the purpose of the period. Click the Add Period button to add another step in the profile. Click the Delete Period button to remove the last step from the profile. As steps are added to the profile, the graphical representation is automatically updated to show the relative speed and time of the complete profile.
Click-to-check the “Loop Profile” box, if you’d like to repeat the profile indefinitely. When checked, you must manually stop the experiment. 5.5.2 Start Profile Mode Experiment
Click the Start button to begin the experiment. A “Save Experiment Data File” window will open.
Enter the name for the data file and click the Save button. The window will close and return to the experiment window. The hardware will initialize and display: “Load Animals and Click OK!!!”
Load the animals into the treadmill and click the OK button. 46 47 5.5.2 Start Profile Mode Experiment (continued) 48 5.5.2 Start Profile Mode Experiment (continued) The program will initialize all of the controllers of the system with the settings from the first period: Step 1. In any period, when the “Shock” is set to “True”, the shock stimulus will be presented to the grids (as long as a specified limit has not been reached) and data will be accumulated for that period. If the “Shock” is set to “False”, then the shock is removed from the grids and no data will accumulate for the period. The “Profile Mode” tab provides additional details about the experiment session: • • • • “-->”: An arrow within the “Step” column of the profile grid indicates which step (period) is currently in use. Step Time: reports the time remaining within the current step. Vertical Red Bar: indicates the current position within the profile. Stop : used to manually terminate an experiment. Necessary when the “Loop Profile” option has been checked. 49 SECTION 6 TREADMILL USER MENU The user menu is used to view and set various parameters which are saved within the Treadmill Controller. Access to the menu is provided by adjusting the front panel controls to a unique setting (typically not encountered through normal operation):
If the Treadmill Controller is under computer control (indicated by the speed setting displayed with “C” instead of “S”), press the “ODOMETER RESET” button to restore control back to the user.
Set the “TREADMILL BELT” toggle switch to “STOP”.
Set the “SPEED” to “88.7” m/min.
Set the “REP RATE” knob to “2Hz”.
Then press the “ODOMETER RESET” button. The screen will display “User Menu” and leave you at the option for automatic speed and distance calibration in the menu. Within the menu, the various controls provide the typical functions: • • • • REP RATE knob: used as an “up/down” selector to navigate the list of menu options. Move up: select: “3Hz” and then back to “2Hz”. Move down: select: “1Hz” and then back to “2Hz”. ODOMETER RESET: used to select the current menu option. When editing, a simple press-and-release will cancel the edit and restore the previous value. You must press-and-hold to save the new setting as current. SPEED knob: used to select a value from a range of values. TREADMILL BELT toggle: used to begin, cancel, and complete the auto-calibration procedure. 50 6.0 Restore Defaults The top option on the list. This is used to restore all of the factory settings saved during it’s calibration performed at Columbus Instruments. This may be necessary during service as to restore a known configuration should for any reason the current settings are unknown or have become corrupt and the Treadmill Controller is non-responsive.
Press the “ODOMETER RESET” button to select the option. “Are you sure?” will be displayed.
Then press-and-hold the “ODOMETER RESET” button to restore the factory settings. Else, pressand-release to cancel. 6.1 Serial Number This is used to see the electronic serial number which should match the Columbus Instruments serial tag on the outside of the unit. For service, this number may be referenced to lookup any specific details or notes about your particular Treadmill and Controller. This option is non-editable. It is provided for reference and additional information. 6.2 Run-Time Meter This is used to report the total amount of time the Treadmill Controller has been powered-on. The format is Days : Hours : Minutes (DHM). To preserve the integrity of the EEPROM (non-volitile storage), the Run-Time Meter is saved every ten minutes. This option is non-editable. It is provided for reference and additional information. 6.3 Run-Time Odometer This is used to report the total accumlated distance (in meters) the treadmill belt has moved. This distance is formated for eaisier viewing. To preserve the integrity of the EEPROM (non-volitile storage), the Odometer is saved every ten minutes. This option is non-editable. It is provided for reference and additional information. 6.4 Unit ID This is used to set the CI-Bus address of the Treadmill Controller. The address should be properly set from the factory, however, this option allows changes as needed. The Unit ID should be unique and not match the address of another device with which it is connected on the same bus. The Unit ID ranges from 1 to 254 (220 typical).
Press the “ODOMETER RESET” button to select the option. The displayed address is now editable.
Use the “SPEED” knob to select the new value for the Unit ID.
Then press-and-hold the “ODOMETER RESET” button to save the new setting. Else, press-andrelease to cancel. 51 6.5 AutoCal Speed This is used to perform an automatic belt speed and odometer distance calibration. You must have the Calibration Wheel to successfully perform this function.
Press the “ODOMETER RESET” button to select the option. The message will display “Run to Start…”
Move the “TREADMILL BELT” toggle from “STOP” to “RUN”. The treadmill belt will move at a speed around 50.0 meters/minute. The display will show: “Stop to Cancel..” and a 15-minute timer which will begin to count down. The automatic calibration procedure can be stopped any time by returning the “TREADMILL BELT” toggle switch back to “STOP”.
Now connect the Calibration Wheel to the Treadmill Controller.
Remove the thumbnuts and remove the treadmill cover and lid.
Place the Calibration Wheel over the threaded stud and secure with one thumbnut. Be sure the wheel is aligned parallel to the running belt. The Calibration Wheel comprises a bent metal frame and therefore may not always parallel with the treadmill lane when the wheel is properly aligned. After the count-down time expires, the Treadmill Controller will check for a speed output from the Calibration Wheel. If detected, then the calibration procedure will continue, else the following message is displayed: “External Wheel Not Detected…”.
Verify the connection. If all appears correct, try cycling the AC power and start the calibration again. Else, contact Columbus Instruments for help. Once an output has been detected the screen will display “Press Odometer Reset…”.
Press the “ODOMETER RESET” button on the front panel of the Treadmill Controller to continue. The treadmill controller will calibrate the low speed of 10.0 meters/minute and then the high speed of 100.0 meters/minute, each within the span of 3 minutes 17 seconds). Lastly, it will calibrate the odometer distance in one minute and display the measured counts from the motor odometer.
Press the “ODOMETER RESET” button to save the new calibration settings. Then the screen will display “Stop to Finish…”
Return the “TREADMILL BELT” toggle switch back to “STOP”. 52 6.6 Acceleration Settings This option is used to set the acceleration rate and ending speed of the treadmill belt when the “TREADMILL BELT” toggle is set to “ACCEL”. The screen displays three adjustable settings: • • • Acceleration Step: Acceleration Interval: Ending Speed: 0.0 to 25.5 meters/minute (m/min). 0 to 255 seconds (s). 0.0 to 102.3 meters/minute (m/min). The Ending Speed (E:), previously known as Maximum Speed, is the target speed to accelerate (or decelerate) towards and maintain once achieved. The acceleration rate (A:) is determined by dividing the Acceleration Step by the Acceleration Interval. Should any of the acceleration arguments be zero, then the acceleration is “OFF” and the default acceleration rate of 100.0 meters/minute/second is employed.
Press the “ODOMETER RESET” button to select the option. The displayed Acceleration Step is now editable.
Use the “SPEED” knob to select the new value for the Acceleration Step.
Then press-and-hold the “ODOMETER RESET” button to save the new setting. Else, press-andrelease to cancel. Now the Acceleration Interval is editable.
Use the “SPEED” knob to select the new value for the Acceleration Interval.
Then press-and-hold the “ODOMETER RESET” button to save the new setting. Else, press-andrelease to cancel. Now the Ending Speed is editable.
Use the “SPEED” knob to select the new value for the Ending Speed.
Then press-and-hold the “ODOMETER RESET” button to save the new setting. Else, press-andrelease to cancel. Now the currently saved acceleration settings are displayed. 6.7 Operating Mode For better stand-alone functionality when providing general-purpose exercise regimens, two new modes of operation have been added. The new modes simply stop the treadmill belt when a set duration of time or distance has elapsed (the same as if the “TREADMILL BELT” toggle were set to “STOP”). The durations are restored and the treadmill belt will start and run when the “ODOMETER RESET” button is pressed. The Operating Mode does not function during a PC-Controlled Experiment. The three modes of operation are: • • • Normal: no limits are employed, the treadmill belt must be manually stopped. Time Limit: a count-down timer will automatically stop the belt. Distance Limit: a count-down odometer will automatically stop the belt. 53 6.7.0 Normal This mode does not employ any limits and is backward compatible with previous versions of the Treadmill Controller. This is indicated by the label “D” for the measured odometer distance which increases as the treadmill belt moves.
Press the “ODOMETER RESET” button to select the option. The displayed mode is now editable.
Use the “SPEED” knob to select “Normal”.
Then press-and-hold the “ODOMETER RESET” button to save. 54 6.7.1 Time Limited This mode limits the time duration of the exercise session. This is indicated when the odometer is replaced by a count-down timer: “T”. The timer will count-down at any belt speed when the “TREADMILL BELT” toggle is set to “RUN” or “ACCEL”. The timer will freeze when “TREADMILL BELT” toggle is set to “STOP” (and the belt speed will also stop) preserving the remaining time duration. When the timer reaches zero (0:00), the belt will stop. At any time, pressing the “ODOMETER RESET” button will reload the timer with the time limit and the treadmill belt will act according to the “TREADMILL BELT” setting.
Press the “ODOMETER RESET” button to select the option. The displayed mode is now editable.
Use the “SPEED” knob to select “Time Limit”.
Then press-and-hold the “ODOMETER RESET” button to save. Now the minutes of the time limit are editable.
Use the “SPEED” knob to select the number of minutes from 0 to 255 (up to 4 hours and 15 minutes).
Then press-and-hold the “ODOMETER RESET” button to save. Else press-and-release to cancel. Now the seconds of the time limit are editable.
Use the “SPEED” knob to select the number of seconds from 0 to 59.
Then press-and-hold the “ODOMETER RESET” button to save. Else press-and-release to cancel. 55 6.7.2 Distance Limited This mode limits the distance travelled in the exercise session. This is indicated by the label “L” for the measured odometer distance which counts down as the treadmill belt moves. When the odometer reaches zero (0.0), the belt will stop. At any time, pressing the “ODOMETER RESET” button will reload the odometer with the distance limit and the treadmill belt will act according to the “TREADMILL BELT” setting.
Press the “ODOMETER RESET” button to select the option. The displayed mode is now editable.
Use the “SPEED” knob to select “Distance Limit”.
Then press-and-hold the “ODOMETER RESET” button to save. Now the 1,000 & 100 digits of the odometer are editable.
Use the “SPEED” knob to select the distance from 0 to 9,900 meters.
Then press-and-hold the “ODOMETER RESET” button to save. Else press-and-release to cancel. Now the 10 & 1 digits of the odometer are editable.
Use the “SPEED” knob to select the distance from 0 to 99 meters.
Then press-and-hold the “ODOMETER RESET” button to save. Else press-and-release to cancel. 6.8 Exit This option is used to exit the menu.
Press the “ODOMETER RESET” button to select the option. The display will show the current settings of the treadmill and the controls will revert back to typical operation. 56 SECTION 7 TREADMILL SOFTWARE DESCRIPTIONS 7.0 Experiment >> Run This option will open the “Run Experiment” window which is used to load, configure, save and run experiments. The following controls are on this form: • • • • • • • • • • • • • • Filename: displays the path and filename of a loaded or saved experiment or the currently running experiment session. Manual tab: see section “7.0.0 Manual Tab”. Profile Mode tab: see section “7.0.1 Profile Mode Tab”. Belt Status: displays the current status of “TREADMILL BELT” toggle during an experiment: “Idle” indicates the toggle is set to “STOP”. “Running” indicates the toggle is set to “RUN”. “Accel” indicates the toggle is set to “ACCEL”. Spd(m/m): reports the current speed of the treadmill belt. Dist Tr (m): reports the total distance the treadmill belt has moved. Belt Angle: reports the current angle of inclination. Status C1 … C8: reports the status of the shock for each channel or lane: Active: The shock is on. The grids are active and monitored for shocks received. Idle: The shock is off. A limit has been reached, either number of visits or shocks. No Shock: The shock is temporarily off for the current step in Profile Mode. Interval Data Grid: o Int: reports the interval. o CH: reports the channel (1 to 8). o Time: the time-stamp when the data was collected. 24-hour format. o Speed (m/m): the speed of the treadmill belt in meters/minute. o Distance (m): the total distance the treadmill belt has moved in meters. o Belt Angle: the current incline of the belt in degrees. o TOB (min): Time On Belt. The minimum accumulated measurement of time when the shock is “Active” and the animal is not detected on the grid. o NOS: Number Of Shocks. the total number of shocks received. o NOV: Number of Visits. the number of visits to the grids when one or more shocks were received. o Status: without Shock Detection reports: “Disabled” if the “Shock Rep_Rate” is set to “Off”. Else it reports “Enabled” when set to “1Hz”, “2Hz” or “3Hz”. with Shock Detection reports: “Idle” when the limits of the NOS or NOV has been reached. Else it reports “Run”. Load : used to load the settings of a previously saved or run experiment. Save : used to save the current experiment settings as a template for future experiments. Start : used to begin an experiment session. Stop : used to terminate an experiment session. Quit : used to close the experiment window and return to the main window. 57 7.0.0 Manual Tab This tab contains the controls used to configure: data collection, the treadmill speed profile in a manual experiment, the treadmill shock stimulus, the shock detection settings and the incline settings for a manual experiment. The controls on the Manual tab are defined as follows: • • • • • • • • • • • • • • • • Interval: defines the interval when data is collected and saved to the output data file. This must be set for both manual and profile mode experiments. Duration: specifies the duration of the experiment session in minutes. This must be set for a manual experiment only. A value of zero will allow the experiment session to run indefinitely, which then must be manually stopped. Enable Profile Mode: click-to-clear to run a manual experiment, else, click-to-check to run a profilemode experiment. The profile mode can provide many steps of speed, acceleration, shock and incline control throughout an experiment session. Accel Step: 0.0 to 25.5 meters/minute (m/m). The numerator of the acceleration rate which defines the change in speed. This control must be directly set in a manual experiment. This setting is automatically updated throughout a profile mode experiment as specified by the profile. Accel Int: 0 to 255 seconds (s). The denominator of the acceleration rate which defines the change in time. This control must be directly set in a manual experiment. This setting is automatically updated throughout a profile mode experiment as specified by the profile. Max Speed: 0.0 to 102.3 meters/minute (m/m). The ending speed or target speed that treadmill belt will accelerate (or decelerate) towards and then maintain once achieved. This control must be directly set in a manual experiment. This setting is automatically updated throughout a profile mode experiment as specified by the profile. S_Speed: 0.0 to 102.3 meters/minute (m/m). The starting speed which is the first target speed the treadmill belt will accelerate toward from rest (0.0 m/m) at the acceleration rate of 2.0 meters/minute/second. Once the starting speed has been reached, the acceleration rate and ending speed are set as defined by “Accel Step”, “Accel Int” and “Max Speed”. This control must be directly set in a manual experiment. Shock Rep_Rate: sets the repetition rate of the shock stimulus from a choice of: Off, 1Hz, 2Hz or 3Hz. This must be set for both manual and profile mode experiments. Shock Intensity: documents the Treadmill Controller’s front panel setting of “INTENSITY” for the shock stimulus which is recorded in the data file. This must be set for both manual and profile mode experiments. Session Number: 1 to 10. Not needed. This setting is included for consistency with the Shock Detection software. Number of Active Channels: 1 to 8. Sets the number of channels/lanes to monitor in an experiment. Typically set to 3 or 6. Number of Visits to Grid: 1 to 999. Limits the number of shocks given to an animal based upon the number of visits to the grid where one or more shocks were received. Number of Shocks: 1 to 999. Limits the number of shocks given to an animal based upon the total shocks received for all visits. Range: reports the valid range the “Belt Angle” may be set in an experiment as indicated by the Incline Controller. Belt Angle: -10.0° to +25.0° (typical). Sets the incline of the belt. In a manual experiment, this control must be directly set which takes effect upon the start of the experiment session. This setting is automatically updated throughout a profile mode experiment as specified by the profile. Inc_Rate: reports the maximum inclination rate of the Incline Apparatus (1.6°/second typical for an Exer 3/6 Treadmill). 58 7.0.1 Profile Mode Tab This tab contains the controls used to define the treadmill belt speed, acceleration, time durations, belt inclination, and shock presentation for each period in the profile. The controls on the Profile Mode tab are defined as follows: • • • • • • 7.1 Loop Profile: Click-to-clear this box to execute the profile once. The total time for the experiment session will be the sum of all the seconds defined in the “Period sec” column. Else, click-to-check this box to repeat the profile indefinitely. The user must click the Stop button to terminate the experiment session. Period Data Grid: o Step: 1 to ?. The unique index for each period defined in the profile. During an experiment, an arrow “-->” appears next to the step which is currently in effect. o Start Speed m/min: 0.0 to 102.3 meters/minute. Used to calculate the change in speed for the acceleration rate for the period. For the first period (1), the setting is always zero (0.0). For subsequent periods, it should match the previous period’s “Ending Speed m/min”. o End Speed m/min: 0.0 to 102.3 meters/minute. Used to calculate the change in speed for the acceleration rate and set the ending speed for the period. o Period sec: 0 to 32767 seconds (9 hours: 6 minutes: 7 seconds). Used to calculate the change in time for the acceleration rate and set the time duration of the period. o Belt Angle: sets the inclination of the treadmill belt at the start of the period. o Shock: enables (True) or disables (False) the shock stimulus (provided the limits of NOV & NOS have not been reached) for the entire duration of the period. o Comment: optional text which can describe the purpose of the period’s settings. Add Period : Used to append one period to the list of defined periods of the profile. It is best to completely define each period before adding the next. Delete Period : Used to delete the last period in the profile. Step Time: During a profile mode experiment, this indicates the remaining time of the current period in effect. Graph: This is a graphical representation of the profile. The dark red lines indicate the calculated speed of the treadmill belt over total time of the profile. The scales of the axes are ratiometric to the largest value of “End Speed m/min” and the sum of the “Period sec”. During an experiment, a vertical bright red bar will indicate the relative position within the profile. Experiment View This option is used to load a previously run experiment and view the collected results within the Treadmill Software program. 59 7.2 Tools >> Device Scan The Treadmill Software keeps a list of configured devices with which it expects are connected to the CIBus and available for an experiment. This option is used to verify the configured devices are present. Also, it can be used to add/remove devices from the list. This option will open a “Communication Scan” window: The following information and controls are available: • “Connected through COM?”: The COM port provided by the CI-Bus Hub where the program found one or more configured devices upon launch of the program. • Device List Grid: list of all configured or discovered devices on the CI-Bus. o Device: Name of the device. o Address#: 1 to 254. The unique CI-Bus address of the device o Prod ID#: 0 to 9999. The Product Number of the device (Columbus Instruments specific). o Version: The firmware revision of the device. o Status: “OK” indicates the device has been detected. “No-Resp” indicates an expected and configured device is absent. • Progress Bar: displays the progress of the “Scan for New Devices” procedure (see below). • Scan Configured Devices : click this button to verify the presence of all configured devices listed. • Scan for New Devices : click this button to discover all devices on the CI-Bus. Their information will display in the device list as they are found. • Exit : click this button to close the window and return to the main window. If changes have been made to the list of devices, the user is prompted to save or discard the changes. 60 7.2.0 Adding Devices to the CI-Bus Use the following procedure to add new devices to the CI-Bus:
Note the addresses of the currently configured and detected devices on the CI-Bus. Typical addresses are: 220, 230 and 235 for the Treadmill Controller, Treadmill Shock Detection unit and the Incline Controller respectively.
Check the addresses of the devices you intend to add to the CI-Bus. Typically, it is printed on the serial tag or may be available to view or edit electronically by the device itself. Each address should be unique and different from all others on the CI-Bus, inclusive of all ports on the CI-Bus Hub.
Remove the orange terminator from the last device on the end of the CI-Bus.
Connect the new devices by way of CAT5 Network cables.
Install the orange terminator in the available “CI-Bus” port of the last device.
Switch “On” the power to all devices.
Click the Scan for New Devices button and program will discover all devices on the CI-Bus.
If all devices are properly detected, click the Exit button. A “Configuration” window will open.
Click Yes to save the changes and return to the main window. 7.2.1 Removing Devices from the CI-Bus Use the following procedure to remove existing devices from the CI-Bus:
Switch “Off” the AC power of the devices to remove.
Click the Scan for New Devices button and program will discover all devices on the CI-Bus.
If all of the expected devices are properly detected, click the Exit button. A “Configuration” window will open.
Click Yes to save the changes and return to the main window. 61 7.2.1 Removing Devices from the CI-Bus (continued) The devices removed from the list of configured devices may still remain connected to the CI-Bus, even powered on. As they are not in the list of configured devices, their presence will not be checked and not used in an experiment. Of course, you may physically remove the devices from the CI-Bus so that they may be used elsewhere:
Switch “Off” the device and disconnect it from AC power.
Disconnect the CAT5 networking cables from the device. If it was the last device on the bus, remove the orange terminator.
Reconnect the CAT5 networking cables as needed and install the orange terminator in the available “CI-Bus” port of the last device. 7.3 Window This menu contains the default options (Cascade, Tile Horizontal, Tile Vertical, Arrange the Icons) Windows provides for positioning open windows within the main window. 7.4 Help This menu contains links to the PDF user manuals of the devices the Treadmill Software expects to control: • Treadmill Manual (this manual) • Shock Detection Manual • Inclinometer Manual 7.4.0 About This reports the version of the Treadmill Software. Be sure to reference the version when contacting Columbus Instruments for help.
Click the OK button to close the window and return to the main window. 62 SECTION 8 MAINTENANCE 8.0 Treadmill Belt Tracking As the treadmill is used, the treadmill belt and the pulleys will begin to wear in. This may cause the belt to stretch and become loose which may also coincide with the belt beginning to track away from the center of the treadmill. Adjustments can be made to return the belt to the center of the treadmill.
Remove the treadmill cover and the lane dividers.
Set the “SPEED” to a high setting, 50.0 meters/minute or greater
Then switch the “TREADMILL BELT” toggle to “RUN”.
Observe the treadmill belt from above the front (the inclination rod has been removed for clarity). Using the 7/16-inch wrench supplied with the treadmill, tighten and loosen the nuts as follows: • If the belt is right of center: tighten the nut on the right and loosen the nut on the left. • If the belt is left of center: tighten the nut on the left and loosen the nut on the right. 63 8.0 Treadmill Belt Tracking (continued) Make small adjustments to the nuts, only 1/4 turns, and allow 1 minute for the belt to settle to the new tracking position. Once the tracking adjustments are complete, follow the procedure in the next section. 8.1 Treadmill Belt Tension If the mechanical sounds of the treadmill appear to have increased in volume or the belt cannot be stopped with a heavy downward pressure, then the overall tension of the belt may be too high.| Check the overall belt tension as follows:
Set the “TREADMILL BELT” toggle switch to “STOP”.
Lift the edge of the belt from the treadmill surface. The belt should NOT be less than 4cm (1.5-inches) from the treadmill surface. If required, loosen both nuts equally until a height of the belt is 4cm. 8.2 Cleaning For safety, switch off and unplug all of the equipment. Remove the cover with lid and all of the lane dividers. All of the parts can be cleaned with a mild soap solution. Do not immerse the treadmill. Try to prevent liquids from entering the components as this may damage bearings or the electronics inside. 64 Serial Number: (Please reefer to this num mber for servicee)                     Exer 3/6 Trea admill Shock S D Detectioon Upgrrade In nstruction n Addend dum 6/2 20/20111                                                               025 57-903L L Copyright C Colu umbus Instrum ments 2011                               Collumb bus IInstrrumentss     950 North Haague Avenue Columbus, Ohio O 43204-212 21 U.S.A. E-mail: [email protected] Phhone: (614) 2766-0861 Fax: (614) 2766-0529 httpp://www.colinstt.com Table of Contents Exer 3/6 Treadmill Shock Detection Upgrade Instructions.         Page   1. COMPUTER SETUP ........................................................................................................................... 3 1.0. Serial Port Installation................................................................................................................... 3 2. INSTALLING SOFTWARE............................................................................................................... 4 2.1 Treadmill Software Installation..................................................................................................... 4 2.2 Removing the Software................................................................................................................. 6 3.     HARDWARE SETUP ...................................................................................................................... 8 3.1 Item Inventory.............................................................................................................................. 8 3.2 Connecting the Computer ……….................................................................................................9   Connecting the Treadmill and Controller to the Shock Detection Interface …………...............10   3.3 4. SOFTWARE SETUP........................................................................................................................... 12 4.1 Treadmill Software Initialization........................................................................................... 12 4.2 Shock Detection Parameters….............................................................................................. 14 4.3 Shock Detection Status and Channel State............................................................................ 14 4.4 Data Generation and Data Output......................................................................................... 14 5. TROUBLESHOOTING......................................................................................................................15 5.1 Hardware………………….....................................................................................................15 5.2 Treadmill Diagnostics ...........................................................................................................16   2 2. INSTAL LLING SOFTWA ARE 2.1. Treadmill So oftware Installlation   You Y must have administrator a rights/privledge r es on the host ccomputer for thhe software to install su uccessfuly. Th he software is expected e to be installed i and ruun locally on thhe host computter’s hard drivee an nd not from a remote r mapped d network resou urce.    Follow these steps to install the Treadm mill software:   Inssert the Treadm mill Software CD-ROM C into the t host compuuter. If the “T readmill Softw ware Setup” app plication does not automaticaally launch, theen you must maanually launch the installationn program.   1. Select “Start » Run...” R from th he desktop taskb bar. A “Run” window will oopen. Browse” windo ow will open. 2. Cliick the Browsee button. A “B       3. 4. 5. 6. Select the CD-RO OM drive from m the “Look in::” combo box. Cliick once to hig ghlight “Setup..exe” from the list of files on the CD-ROM.. Cliick the Open button. b The “B Browse” window will close annd return focuss back to the “R Run” window. Cliick the OK bu utton to begin th he installation program. p          Upon initiallization, some install files willl be processedd and copied too the host compputer. These files will bee removed if seetup is cancelleed. Then a bluee “Treadmill Software Setup”” backdrop andd installation window will be b displayed. 4 2.1. Treadmill So oftware Installlation (continu ued)   7. Cliick the OK bu utton to continu ue.                             8. Cliick the square button b marked d with a com mputer and flo oppy disks to co ontinue.                     9. Cliick the Continu ue button to beegin the copy and a insstallation proceedure into the default d Program m Group.                                   The in nstall program will w check for necessary n disk k space and thenn begin to copyy the program.. It is recom mmend to keep any a files on yo our system that are newer thann the ones beinng copied. Upoon completion,, the pro ogram will upd date the Windo ows system and d create program m icons inside the group foldder on the start menu.   10. Cliick the OK bu utton to finish th he install progrram. Th he “Treadmill Software S Setup p” screen will close c and returnn to thee user desktop.   5 2.2. Removing th he Software   Remov ving the Tread dmill Software application is required r when upgrading to a newer versionn of the application or relocaating the application to a new host computerr. When upgraading, this ensuures that all of the sup pport files pack kaged with the application are copied and innstalled to the hhost computer.   Follow w these steps to t remove the Treadmill Sofftware:   1. Select “Start » Control C Panel” ” from the desk ktop taskbar. T This will open the “Control P Panel” window..       2. Ru un the “Add or Remove Progrrams” applicatiion. This will open the “Addd or Remove Prrograms” window. nder the “Chan nge or Remove Programs” selection, scroll thhrough the listt of installed prrograms and 3. Un click once to high hlight “Treadm mill Software”. Treadmill Softw ware Removal”” 4. Cliick the Changee/Remove buttton. The PC sccreen will channge to a blue “T scrreen.     6 2.2 Removing the Software (co ontinued)   5. Cliick Yes to beg gin the removall of the Treadm mill Software prrogram.       6. Cliick OK to closse the “Applicaation Removal”” window and rreturn to the “A Add or Removve Programs” window.     7. Cliick X in the top-right t corneer of the “Add or o Remove Proograms” windoow to close. 8. Cliick X in the top-right t corneer of the “Contrrol Panel” winddow or select ““File >> Close”” from the meenu bar.     7 3. HARDW WARE SE ETUP Connecting th he Shock Deteection Interfacce to the Tread dmill   3.1 Item Invento ory Treadm mill Shock Dettection compon nents included with the shipm ment are as picctured below. T The following instrucctions make usse of these parts and will referrence parts inccluded with thee treadmill wheen it was originally purchased d. If any of the original compo onents are misssing, inquire w with us for replaacements.                                nductor, plug-pplug. 1 x RJ-45 cable,, 38cm (15 inch), 8-pin 8-con k cable, 38cm (15-inch), ( 15-p pin 15-conducttor male-male. 1 x D-Sub shock d cablee. 1 x 25-pin malee-male shock detection The Shock Deteection Interfacce. m also need the t following to ools for assemb bling the system m: You may  Ph hillips (cross-p point) screwdriv ver, medium tip.  Common (flat-p point) screwdriv ver, small tip.  5//16-inch (7.5m mm) wrench.     Note: It is recommeended to use an n AC power strip to provide a common shorrt-distance acceess to AC powerr for all compon nents of the Trreadmill system m. Most AC poower strips incoorporate a masster switch that can sw witch on and offf the AC poweer to the entire system and prootect against ellectrostatic disccharge. It alsoo provid des a beneficiall common grou und between all components tto help preventt unintentional ground loops.   8 3.2 Connecting the t Computer Interface The co omputer interfa face and cabless were included d originally wiith the treadmiill. If you alreaady have the treadm mill connected to a PC, or do o not wish to usse the softwaree, you can skipp this step and pproceed to section n 3.3.   1. 2. Connect the fem male end of the RS-232 cable to a free seriall port on the hoost computer (nnot shown). Connect the malle end of the RS-232 R cable to o the “COMPU UTER” port of tthe Computer IInterface. 3. 4. Connect the pow wer plug of the power supply to the “POWE ER” port of the Computer Inteerface. In nstall the power supply into th he appropriate AC outlet (nott shown). The power indicatoor (top right grreen LED on th he “CONTROL LLER” port) will w light continnuously. 5. Connect one end d of the RJ-45 cable to any off the “CONTR ROLLER” portss of the Compuuter Interface.                 9 3.2 6. Connecting the t Computer Interface (Co ontinued…) Connect the otheer end of the RJ-45 R cable to any a of the “CIB BUS”” ports off the Treadmilll Controller.       3.3 Connecting C thee Treadmill an nd Controller to the Shock D Detection Inteerface Pllace the Shock k Detection Inteerface on top of o the treadmilll controller in such a mannerr that you havee acccess to the reaar panels of both. Disconnectt the power corrd from the treeadmill controlller for safety. 2. Remove the flat,, black, 15-pin shock cable (A A) that presentlly connects the treadmill to thhe controller annd seet it aside. It wiill not be used in i the shock deetection setup. N Note: nothing w will connect too the port labeleed B on the treadmiill in the shock k detection setu up. 1. A A B Using U the sho orter D-sub sho ock cable thatt came 3. with th he Shock Deteection Interfacee, connect one end to the Trreadmill Conto oller in the porrt labeled “Sho ocker.” The other o end, co onnect to the Shock Dettection Interfaace labeled “To o Treadmill.”   10 4. Con nnect the 25-piin cable to the Shock Detectio on Interface to the port labeleed “To Shockerr Unit.” The othher end will connect to thee side panel off the treadmill ittself, as illustraated below. 5. Th he short black RJ-45 cable th hat came with the t Shock Deteection Interfacee will connect the Shock Detecttion Interface to t the Treadmilll Controller viia the ports labbeled “CI-Bus.”” Shock cables removedd for illustration ppurposes. 6. Reconnect the AC C power cord to t the rear paneel AC power reeceptacle of thee Treadmill Coontroller and d insert the oth her end into an appropriate AC C outlet.         11 4.0 SOFTW WARE SE ETUP     4.1. Treadmill So oftware Initiallization   From the t desktop tasskbar, select “S Start » All Pro ograms » Treaadmill Softwarre » Treadmilll Software”.      Upon initializaation, the softw ware will autom matically scan tthe Treadmill C Controller’s avvailable serial communicatio on ports (COM)) for existing devices d and associated addresss identificationn numbers.  The starting deevice identificaation address (D DEVID 220) iss assigned to thhe Treadmill C Controller(s) with additionaal addresses folllowing numeriically for multiiple devices.  The starting deevice identificaation address (D DEVID 230) iss assigned to thhe Shock Contrroller(s) in thiss same manner.   To resscan the Treadm mill Controllerr for the presence or addition oof connected ddevices, select ““Tools » Device Scan” from the t Treadmill Software S main window.    Click the “Scaan for New Dev vices” button to o add any deviices previouslyy not configuredd. D butto on to rescan devvices already ppresent in the laatest  Click the “Scaan Configured Devices” configuration file.  Click on the “E Exit” button to o complete the process p and cloose the “Comm munication Scaan” window.   After closing c the win ndow, an “Upd date Device Co onfiguration” prompt will apppear        Click the “Yees” button to saave the new dev vice configurat ation. o” button to keeep the previouss device configguration.  Click the “No 12 4.1 System Initia alization (conttinued)   Upon successful iden ntification the Treadmill T Con ntroller by the aapplication, thee host COM poort and Treadm mill Controllerr information are displayed att the top of the window as shoown below:     No Deevice Connecteed?   If the Treadmill T Con ntroller could no ot be found, th he following meessage is displaayed:     To re--attempt devicee connection affter a failed com mmunication,  Click the Scan n Ports button to t automaticallly search all avvailable serial ((COM) ports foor the Treadmill Con ntroller. d Users when th he device addreess is already kknown,  For Advanced o Seelect the “Manu ually Select Ad ddress” radio buutton. o Ch hoose the correect device numb ber from the puull-down menuu. o Click the Scan Ports button to scan an individdual port. No Device Founnd!” prompts seee the  If multiple atteempts at devicee connection sttill result in “N Troubleshootin ng section of th his manual. m cannot be rem medied by the su uggested troubbleshooting tipss, contact Coluumbus  If the problem Instruments.       13 4.2 Shock detection n parameters O Sesssion or experiment number. O Nu umber of activee channels with h a range of (1--8). For the Exxer 3/6 Treadm mill System the maximum num mber of active channels is 3 for f rat subjects and 6 for micee subjects. O Nu umber of visits to grid (NOV)) contains a ran nge of (1-999). This sets the nnumber of timees the subject maakes contact with the grid befo fore it is disableed. O Nu umber of shock ks (NOS) also has h a range of (1-999). ( This aallows the userr to set the num mber of times thee subject receiv ves a shock beffore the grid is disabled.     4.3 Shock Detectio on Status and Channel C Statee  The numberr of active chan nnels (C1-C6) is represented by virtual LED Ds within the status bar.   “Active” ch hannels denote the presence of o electrical stim mulus and are iindicated by thhe green LEDs. “Idle” chan nnels denote lacck of electrical stimulus and aare indicated byy the red virtuaal LEDs.     4.4. Data D Generatiion and Outpu ut As exp perimental dataa is generated it i is displayed in i columnar foorm and can be saved as a com mma delimited (.csv) file for analysiis in Microsoft® Excel or sim milar spreadsheeet applicationss.     mn heading descriptions are ass followed, Colum  Interval (Int): Interval as en ntered within the t interval andd duration settiing. H): Channels seelected within Shock Detectioon settings.  Channel (CH  Time: Time of o day in 24-ho our format in which w experimeent interval wass recorded.  Time on Beltt (TOB): Reco orded time in which w subject w was on the treaddmill belt and nnot the grid. d (m/s) recordeed for experimeent interval.  Speed: Speed d experimental interval  Distance: Disstance in meterrs for specified   14  Number of Shocks (NOS): The recorded number of shocks administered by the grid.  Number of Visits (NOV): The number of recorded visits to the grid within an interval.  Status: Status of treadmill belt indicating RUN or IDLE.   5.0 TROUBLESHOOTING   5.1 Hardware   The following tips might aid in identifying hardware issues:                Check the AC outlets for power. Does a switch control power to the outlet? Are you using a DB25-DB9 serial adapter with your serial port? Some of these are made specifically for connecting mice and modems to the computer that may interfere with the communication of the CIBus. Contact Columbus Instruments if uncertain. Check the power indicator on the Computer Interface. The top right green LED on the “CONTROLLER” port should be lit continuously. Check the Power Supply for the Computer Interface. Is the power plug installed correctly in the Computer Interface? Is the power supply inserted into AC power? Check the AC power cord. Is it fully inserted into the Treadmill Controller and AC power? Check the fuses of the Treadmill Controller (it may require the use of an ohmmeter to verify conductivity). Check the LCD of the Treadmill Controller. It should be lit continuously. There should be text on the LCD (adjust the contrast knob fully clockwise to view). If not, contact Columbus Instruments. This may be an indication of a fault within the Treadmill Controller. Check the serial cable between the host computer and the Computer Interface? When properly connected, the transmit indicator (top left yellow LED on the “CONTROLLER” port of the Computer Interface) will flash very briefly when the computer sends information to the CI-Bus. Likewise, the receive indicator (top middle green LED on the “CONTROLLER” port of the Computer Interface) will flash briefly when any device sends information to the CI-Bus. Check the RJ-45 cable between the Computer Interface and the Treadmill Controller. It should be fully inserted with the connector locking tab snapped into the jack.   15 5.2 Treadmill T Diag gnostics   The Treadmill Diagn nostics providees additional co ontrols for monnitoring the treaadmill system w while an experiiment is active.. To access the Diagnostics window, w select “Tools » Treaadmill Controll” from the Treadm mill Software main m window.   The window w is divid ded into 3 sectio ons.  The top section displays inform mation about th he Treadmill C Controller and tthe serial port co ommunicationss.  The middle section provides co ontrols for read ding and writinng the accelerattion parameterrs. r the curren nt treadmill beltt speed and disstance traveled.  The bottom secttion is used to read     Notes: Values outsid de the ranges listed may not be b accepted andd may cause the application too close. Fractional vallues for “Accell Step” and “M Max Speed” musst be entered w with the decimaal point (.). Acceleration will w be zero if “Accel “ Step” = 0.0 or “Accell Int” = 0. “Max Speed” limits the tread dmill belt for “RUN” “ and “A ACCEL” modess of the Treadm mill Controller..       16 1. 2. Click the Write button to apply the settings and save them m to the Treadm mill Controller. Click the Read button to read d the settings currently savedd in the Treadm mill Controller. otes: The accelleration parameeters entered in n the text boxess will not be appplied and becoome current No until the Write buttonn is clicked.             The Clear C Dt button will clear thee odometer on the t Treadmill C Controller (thee same as the push button). The Read R Speed bu utton will updaate the Belt Status, Spd(m/minn) and Dist Tr (m).  Belt Status: “Idle”, “Runn ning” and “Acccel” are reporteed for treadmilll belt modes “STOP”, “ “RUN N” and “ACCE EL” respectivelly.  Spd(m/min)): the speed dissplayed by the Treadmill Conntroller. t record ded by the odom meter.  Dist Tr (m): the distance traveled   If the “Continuous” “ option o is check ked when the Read R Speed bbutton is clickeed, then the upddate will repeaat every 1 second. To stop s the contin nuous update, click to uncheckk the “Continuuous” option annd click the Read Speed S button again. a   17 Serial Number:__________________ (Please refer to this number for service) Automated Incline Controller Setup & User Guide 07/10/2014 Incline_140710 Copyright Columbus Instruments 2014 Columbus Instruments 950 North Hague Avenue Columbus, Ohio 43204-2121 U.S.A. E-mail: [email protected] Phone: (614) 276-0861 Fax: (614) 276-0529 http://www.colinst.com Table of Contents Automated Incline Controller Setup and User Guide Section Page Table of Contents 1 1. Introduction 1.0 System Overview 1.1 System Specifications 1.1.0 Incline Controller 1.1.1 Generic Incline Apparatus 1.1.2 Metabolic Mouse Incline Apparatus 1.1.3 Metabolic Rat Incline Apparatus 1.1.4 Exer 3/6 Incline Apparatus 1.1.5 Option: Incline Sensor 1.2 Calculation of Data 1.2.0 Angle of Inclination 1.2.0 Rate of Change of Angle of Inclination 3 3 4 4 4 4 4 5 5 6 6 6 2. Software Setup 2.0 Treadmill Software Installation 2.1 Removing the Software 7 7 9 3. Hardware Setup 3.0 Metabolic Mouse/Rat 3.1 Exer 3/6 3.1.0 Leveling the Incline Apparatus 3.2 Connecting the Components 3.2.0 With the Treadmill Controller 3.2.1 With the Treadmill and Shock-Detection Controllers 11 12 17 20 27 27 28 4. System Initialization 4.0 Hardware Monitoring 4.0.0 Fuses 4.0.1 Motor Type 4.0.2 Motor Feedback 4.0.3 Positional Mismatch 4.1 Stop Mode 4.2 Run Mode 4.3 Step Mode 29 29 29 30 30 31 31 31 31 5. Computer-Controlled Experiment 5.0 Treadmill Software Initialization 5.1 Device Scan 5.2 Experiment Parameters 5.2.0 Manual Tab 5.2.1 Profile Tab 33 33 34 35 36 37 6. Maintenance 6.0 Cleaning 39 39 1 2 SECTION 1 INTRODUCTION 1.0 System Overview The Columbus Instruments’ Automated Incline Controller has been designed to provide manual or computer-controlled inclination of any apparatus over a practical 100° range, inclusive of 0°. Rotary knobs are provided to select the angle of inclination (or declination) with 0.1° precision and the rate of change of the incline with 0.1°/second resolution. A mode switch selects the method in which to apply those settings. The range of inclination and rate are preconfigured at the factory based upon the mechanics/geometry of incline apparatus. A 2-line LCD display reports the commanded angle of inclination, the rate of change of inclination, the current mode of operation and the incline apparatus’ angle of inclination. In the “RUN” mode, the settings of incline and rate are applied continuously to the incline apparatus. In the “STEP” mode, the settings of incline and rate are applied immediately after the “APPLY” button has been pressed. This allows the user to manually present an exercise profile of inclination at specifically timed intervals throughout an experiment. The controller utilizes two linear-actuators, which work in tandem, to lift/move the incline apparatus. Due to the various sizes and gear-ratios employed in their construction, heavy loads and fast rates of inclination are all possible. The inclusion of separate safety fuses for the motor and continuous monitoring of the linear-actuator’s position and electrical power results in a very robust motion control solution for any automated incline system. The Treadmill Software (or any other Columbus Instruments’ application) can communicate with the Incline Controller by way of the “CI-Bus”. This is a hardware and serial communication protocol that all new and revised instruments incorporate. It allows all products to connect together using standard category 5 network/communication cables (typical for LANs, Internet, etc.). Now the host computer can simultaneously apply changes to the inclination and rate concurrently with the treadmill speed and acceleration, which are all logged at specified intervals throughout an experiment session. Summaries of the features are as follows:        The Incline Controller can control any Incline Apparatus over a practical 100° range inclusive of 0°. The angle of inclination has a resolution of 0.1°. The rate of change of inclination has a resolution of 0.1°/second. The inclination can be adjusted continuously or in steps. The dual linear-actuators are capable of positioning heavy loads at fast rates. The “STEP” mode allows the setup and application of inclination profiles in a manual experiment. The treadmill software allows the setup and application of inclination profiles concurrently with the treadmill speed and acceleration in a computer-controlled experiment. 3 1.1 System Specifications 1.1.0 Incline Controller Power Requirements: Power Fuses: Physical Dimensions: Physical Weight: CI-Bus Connection: Motor Fuses: Motor Connection: 1.1.1 Generic Incline Apparatus Motor Type: Actuator Strokes: Actuator Rates: Angular Range: Resulting Angular Rates: Physical Dimensions: Physical Weight: 1.1.2 60 watts, 100 - 240VAC, 50/60Hz. 2A Slow-Blow 13.0” x 11.4” x 4.0” (33.1 x 29.0 x 10.2cm). 5.8 pounds (2.6 kilograms). 8p8c “RJ45” Jacks (x2). 2A Slow-Blow 6-pin Amphenol Circular Female Jack. Linear-Actuator, 12V. 4”, 6”, 8” 10” or 12” (10cm, 15cm, 20cm, 25cm, 30cm). 0.3”/s, 0.5”/s, 2.0”/s (0.7cm/s, 1.2cm/s, 5.0cm/s). Span of 100° inclusive of 0°. Dependant upon the mechanics/geometry of the Incline Apparatus. 10” (25cm) wider than the apparatus to incline. 4” (10cm) taller than the length of linear-actuator employed. 15 - 25 pounds (6.8 – 11.3 kilograms). Metabolic Mouse Incline Apparatus Linear-Actuator, 12V, 6” Stroke. -10.0° to +25.0°, ±0.1° or -17.6% to +46.6% incline ±0.2%. Actuator Rates: 0.3”/s, 0.5”/s, 2.0”/s (0.7cm/s, 1.2cm/s, 5.0cm/s). Resulting Angular Rates: 1.7°/s, 2.9°/s, 11.6°/s. Physical Dimensions: 8” (20cm) wider than all of the mouse lanes assembled 11” (28cm) long and 23” (58cm) tall. Physical Weight: ~ 16 pounds (7.3 kilograms). Motor Type: Angular Range: 1.1.3 (0.5”/s default) (2.9°/s default) Metabolic Rat Incline Apparatus Linear-Actuator, 12V, 8” Stroke. -10.0° to +25.0°, ±0.1° or -17.6% to +46.6% incline, ±0.2%. Actuator Rates: 0.3”/s, 0.5”/s, 2.0”/s (0.7cm/s, 1.2cm/s, 5.0cm/s). Resulting Angular Rates: 1.2°/s, 2.1°/s, 8.6°/s. Physical Dimensions: 8” (20cm) wider than all of the rat lanes assembled 14” (36cm) long and 26” (66cm) tall. Physical Weight: ~ 19 pounds (8.6 kilograms). Motor Type: Angular Range: 4 (0.5”/s default) (2.1°/s default) 1.1.4 Exer 3/6 Incline Apparatus Linear-Actuator, 12V, 10” Stroke -10.0° to +25.0°, ±0.1° or -17.6% to +46.6% incline, ±0.2%. Actuator Rates: 0.3”/s, 0.5”/s, 2.0”/s (0.7cm/s, 1.2cm/s, 5.0cm/s). Resulting Angular Rates: 0.9°/s, 1.6°/s, 6.6°/s. Physical Dimensions: 26” (66cm) wide, 24” (61cm) long and 31” (79cm) tall. Physical Weight: around 18 pounds (8.2 kilograms). Motor Type: Angular Range: 1.1.5 CI-Bus Incline Sensor Power Requirements: Power Connection: Physical Dimensions: Physical Weight: CI-Bus Connection: Angular Range: +7-12VDC @ 150mA. Coaxial Plug (-) 5.5mm O.D. x (+) 2.1mm I.D or CI-Bus. 3.5” x 2.3” x 1.5” (8.9 x 5.8 x 3.8cm). 3.4 ounces (97grams). Dual 8p8c “RJ45” Jacks. -90.0° to +90.0°, ±0.1° 5 (0.5”/s default) (1.6°/s default) 1.2 Calculation of Data The Incline Controller utilizes linear-actuators with built-in feedback potentiometers. Each actuator contains an electric motor, which turns an ACME ball-screw through a gear transmission. The ACME ballscrew is threaded into a rod which extends outside of the actuator assembly. The position of the rod is sense by the resistive potentiometer. The potentiometer is incorporated into an electronic circuit whose output is in direct proportion to the position of the rod. The Incline Controller employs an on-board microcontroller with many faculties:  12-bit analog-to-digital (A/D) converter.  High-resolution 32MHz system oscillator.  32-bit floating-point arithmetic.  Up to 9th-order polynomials for feedback linearization. Likewise, there are various other circuits which monitor the status of the linear-actuators that can halt their operation automatically or at the user’s request. 1.2.0 Angle of Inclination At the factory, the incline apparatus is setup on a flat and level surface. Utilizing the Columbus Instruments’ Incline Sensor (electronic inclinometer with 0.01° resolution), the extremes of the range of inclination and zero are discovered. These three data points are then linked to the exact positions of the each linear-actuator’s feedback signal as measured by the A/D converter circuitry. Along with a characteristic polynomial equation (based upon the geometry of the incline apparatus) the controller calculates the necessary position of each linear-actuator to drive the incline apparatus to the desired angle of inclination. 1.2.1 Rate of Change of the Angle of Inclination The 32MHz system oscillator is used to derive a 1-millisecond clock signal. Based upon 16-bit fractional math, the dwell-time (in fractional milliseconds) are calculated and allowed to elapse between steps (0.1°) of the angle of inclination. 6 SECTION 2 SOFTWARE SETUP 2.0 Treadmill Software Installation You must have administrator rights/privledges on the host computer for the software to install successfuly. The software is expected to be installed and run locally on the host computer’s hard drive and not from a remote mapped network resource. Follow these steps to install the Treadmill software:  Insert the Treadmill Software CD-ROM into the host computer. If the “Treadmill Software Setup” application does not automatically launch, then you must manually launch the installation program.   Select “Start >> Run...” from the desktop taskbar. A “Run” window will open. Click the Browse button. A “Browse” window will open.     Select the CD-ROM drive from the “Look in:” combo box. Click once to highlight “Setup.exe” from the list of files on the CD-ROM. Click the Open button. The “Browse” window will close and return focus back to the “Run” window. Click the OK button to begin the installation program. Upon initialization, some install files will be processed and copied to the host computer. These files will be removed if setup is cancelled. Then a blue “Treadmill Software Setup” backdrop and installation window are displayed. 7  Click the OK button to continue.  Click the square button marked with a computer and floppy disks to continue.  Click the Continue button to begin the copy and installation procedure into the default Program Group. The install program will check for necessary disk space, then begin to copy the program. It is recommend to keep any files on your system that are newer than the ones being copied. Upon completion, the program will update the Windows system and create program icons inside the group folder on the start menu.  Click the OK button to finish the install program. The “Treadmill Software Setup” screen will close and return focus back to the Windows desktop. That completes the copy and installation of the Treadmill Software. 8 2.1 Removing the Software Removing the Treadmill Software application is required when upgrading to a newer version of the application or relocating the application to a new host computer. When upgrading, this ensures that all of the support files packaged with the application are copied and installed to the host computer. Follow these steps to remove the Treadmill Software:  Select “Start >> Control Panel” from the desktop taskbar. This will open the “Control Panel” window.  Run the “Add or Remove Programs” application. This will open the “Add or Remove Programs” window. Under the “Change or Remove Programs” selection, scroll through the list of installed programs and click once to highlight “Treadmill Software”. Click the Change/Remove button. The PC screen will change to a blue “Treadmill Software Removal” screen.   9  Click Yes to begin the removal of the Treadmill Software program.  Click OK to close the “Application Removal” window and return to the “Add or Remove Programs” window.   Click X in the top-right corner of the “Add or Remove Programs” window to close. Click X in the top-right corner of the “Control Panel” window or select “File >> Close” from the menu bar to close. That completes the removal of the Treadmill Software. 10 SECTION 3 HARDWARE SETUP It is recommended to use an AC power strip to provide a common short-distance access to AC power for all components of the Treadmill, Incline and other systems. Most AC power strips incorporate a master switch that can switch on and off the AC power to the entire system and protect against electrostatic discharge. It also provides a beneficial common ground between all components to help prevent unintentional ground loops. The Automated Incline system will contain the following common items:      1 - Incline Controller with power cord. 2 - motor extension cables. 2 - linear-actuators with hardware: o 2 - 1/4" x 3” quick release pins. o 2 - 1/4"ID, 1/2"OD x 1” long spacers. 1 - instruction manual. with computer control: o 1 - Software CD-ROM. o 1 - 1’ (30cm) CI-Bus data cable. 11 3.0 Metabolic Mouse/Rat Additional hardware for a Metabolic Mouse or Rat system will contain the following items: Two-Lane Modular Mouse shown, all others similar.      1 - Left-Support with hardware: o 2 - 1/4"-20 socket-cap screws, 1/2" long. 1 - Right-Support. 1 - Top-Brace with hardware: o 2 - 1/4"-20 socket-cap screws, 1-1/4” long. o 4 - 1/4" flat washers. o 2 - 1/4"-20 acorn nuts. 1 - Actuator-Brace with hardware: o 2 - #10-24 socket-shoulder screws, 2-1/2” long. o 2 - 1/4"ID, 1/2"OD x 1” long bronze bushings. o 2 - 1/4"ID, 1/2"OD x 1/4" long spacers. 1 - 7/16” open-ended wrench. You will also need:  3/16” Hex Key (comes with metabolic treadmill).  1/8” Hex Key (comes with metabolic treadmill). 12  Position the left-support, modular lanes, right-support and motor assembly as shown. The hexagonal aluminum spacers, which normally connect the motor assembly to the first lane, have been replaced by the right-support.  Using the 1/4"-20 socket-cap screws, 1/2" long, connect all of the pieces together, but DO NOT fully tighten. The screws should hold the pieces closely together AND allow them to move.  Attach the top-brace to the supports as shown. Again, DO NOT fully tighten the screws & nuts. Front View  Back View With all of the components loose and sitting flat on the working surface, begin to fully tighten the screws, starting with the motor assembly and finishing with the left-support. Then tighten the topbrace. Be sure that the brace sits flat against the top of the supports. 13  Prepare each support with one 1/4" x 3” quick-release pin and 1” spacer. Insert the quick-release pin through the support and spacer as shown.  Place the left-actuator, actuator-brace and right-actuator, front-side down, as shown.  Place one bronze-bushing and 1/4" spacer over the shoulder of a #10-24 socket-cap shoulder screw, 21/2” long. Then pass the shoulder through the hole in the left-actuator and into the actuator-brace. Fully tighten with the 1/8” hex key. Repeat the above procedure for the right-actuator.  14  Insert the actuator-brace assembly into the vertical slots of the supports by tilting one end into the bottom of the left slot, then dropping the other end into the top of the right slot.  Let one actuator rest on the working surface while the other is raised to vertical. Then lift the vertical actuator to the top-brace. Once aligned, fully insert the quick-release pin through the actuator. Raise the other actuator to vertical and fully insert the quick-release pin through the actuator and topbrace.  15  Pass the motor cables through the split-rings at the end of the quick-release pins to hold cables away from the metabolic lanes when installed. This completes the assembly of the Metabolic Automated Incline Apparatus. Add the metabolic lanes in the typical manner, motor side first (right-support). Then jump to section: “3.2 Connecting the Components”. 16 3.1 Exer 3/6 Additional hardware for the Exer 3/6 system will contain the following items: (The tops of the supports are not shown for clarity)        1 - Left-Support and Pivot-Block with hardware: o 2 - #6-32 pan-head screws, 1-5/8” long. o 2 - #6 flat washers. o 2 - #6 lock-nuts, 5/16” head. 1 - Right-Support with Pivot-Block. o 2 - #6-32 pan-head screws, 1-5/8” long. o 2 - #6 flat washers. o 2 - #6 lock-nuts, 5/16” head. 1 - Bottom-Brace with hardware: o 2 - 1/4"-20 socket-cap screws, 1-1/4” long. o 2 - 1/4" flat washers. 1 - Top-Brace with hardware: o 2 - 1/4"-20 socket-cap screws, 1-1/4” long. o 4 - 1/4" flat washers. o 2 - 1/4"-20 acorn nuts. 1 - Actuator-Brace with hardware: o 2 - #10-24 socket-shoulder screws, 3-1/2” long. o 2 - 1/4"ID, 1/2"OD x 1” long bronze bushings. o 2 - 1/4"ID, 1/2"OD x 1/4" long spacers. o 2 - 1/4"ID, 1/2"OD x 1-1/4” long Exer 3/6 bushings. 1 - 3/16” Hex Key. 1 - 1/8” Hex Key. You will also need:  7/16” open-ended wrench (comes supplied with the Exer 3/6 treadmill).  Cross-point (Phillips) screwdriver and 5/16” open-ended wrench or pliers.  2-foot (60cm) bubble level (optional) 17  Position the left-support, bottom brace and right-support as shown.  Raise the left-support to vertical. Using a 1/4"-20 socket-cap screw, 1-1/4” long with a flat washer over the threads, connect the left support to the bottom brace. DO NOT fully tighten. The screws should hold the pieces closely together and allow them to move. 18  Repeat the above procedure for the right-support.  Attach the top-brace to the supports as shown. Again, DO NOT fully tighten the screws & nuts. Front View Back View 19 3.1.0 Leveling the Incline Apparatus (optional) If the working surface is flat and level, skip this section. Else, the bottom of the apparatus utilizes threaded feet which can be used raise the corners of the apparatus to level. Using the 2’ bubble level, determine the highest corner of the apparatus:  If the highest corner is in the back (the pivot-block end):  Raise the corresponding front end on the same side to level.  If the highest corner is in the front (support end):  Raise the corresponding back end on the same side to level.  Place the 2’ level across the front of the apparatus and raise the lower front-corner to level.  Then place the level along the same side and raise the rear corner level. Recheck all sides to verify the apparatus is level. 20 3.1 Exer 3/6 (continued)  Fully tighten the screws, starting with the bottom-brace and finishing with the top-brace. Be sure that the top-brace sits flat against the top of the supports.  Prepare each support with one 1/4" x 3” quick-release pin and 1” spacer. Insert the quick-release pin through the support and spacer as shown.  Place the left-actuator, actuator-brace and right-actuator, front-side down, as shown. 21  Using one #10-24 socket-cap shoulder screw, 3-1/2” long, place one bronze-bushing and one 1/4" long spacer over the shoulder. Then pass the shoulder through the hole in the left-actuator. Place one Exer 3/6 bushing over the shoulder (large diameter end toward the threads) and into the actuator brace. Tighten with the 1/8” hex key.  Repeat the above procedure for the right-side.  Insert the actuator brace assembly into the vertical slots of the supports by tilting one end into the bottom of the left slot, then dropping the other end into the top of the right slot. 22   Let one actuator rest on the working surface while the other is raised to vertical. Then lift the vertical actuator to the top-brace. Once aligned, fully insert the quick-release pin through the actuator. Raise the other actuator to vertical and fully insert the quick-release pin through the actuator and topbrace.  Pass the motor cables through the split-rings at the end of the quick-release pins to hold cables away from the Exer 3/6 treadmill when installed.  If installed, remove the two rear rubber feet or the declination adapter from the treadmill. You will require a 5/16” wrench (or pliers) and cross-point (Phillips) screwdriver. 23  Rotate the pivot blocks so they extended towards the rear, away from the bottom-brace.  Lift the entire treadmill from the rear and place the front of it through the supports and allow it to rest on top of the Exer 3/6 bushings. Then lower the rear onto the pivot blocks. Be sure the large-diameter ends of the Exer 3/6 bushings are inside of the rails of the treadmill. 24  Secure the rear of the treadmill to the pivot-blocks by passing a #6-32 pan-head screw, 1-5/8” long, through each of the four holes. Place a #6 flat-washer and #6 lock-nut over the threads and fully tighten. (one of four shown) This completes the assembly of the Exer 3/6 Automated Incline Apparatus. 25 26 3.2    Connecting the Components Place the Incline Controller near the Incline Apparatus. Using the two 6’ motor extension cables, connect the left and right-actuators to the “Left” and “Right” actuator ports on the rear of the controller. Connect the AC power cord to the rear panel AC power receptacle of the Incline Controller and insert the other end into an appropriate AC outlet. 3.2.0 With the Treadmill Controller It is recommended to place the controller cabinets on top of each other. In this example, the lighter Incline Controller is atop the heavier Treadmill Controller.  Setup all of the controllers as indicated in their respective manuals.  Using the blue CI-Bus data cable, connect the available “CI-Bus” port of the Treadmill Controller to one of the “CI-Bus” ports on the Incline Controller. 27 3.2.1 With the Treadmill and Shock-Detection Controllers It is recommended to place the controller cabinets on top of each other. In this example, The Treadmill Controller is placed on the bottom as it is the most heavy. The Shock-Detection Controller is placed in the middle, adjacent to the Treadmill Controller as it utilizes a short cable for connection of the shock signals. The Incline Controller is placed on top as it only requires access to the CI-Bus.  Setup all of the controllers as indicated in their respective manuals.  Using the blue CI-Bus data cable, connect the available “CI-Bus” port of the Treadmill Controller to one of the “CI-Bus” ports on the Shock-Detection Controller.  Using another blue CI-Bus data cable, connect the available “CI-Bus” port of the Shock-Detection Controller to one of the “CI-Bus” ports on the Incline Controller. This completes the hardware setup of the Automated Incline Apparatus 28 SECTION 4 SYSTEM INITIALIZATION Attention! To prevent the unintentional operation of the Incline Apparatus: Select the “STOP” mode prior to the application of AC power.  Switch “On” the Incline Controller. The power switch is just above the power receptacle on the rear panel. The front panel should light up and report “Power-On Reset” for 1 second. Then report the “Apparatus Type” for an additional second. Then display the main screen. 4.0 Hardware Monitoring The Incline Controller continuously monitors the safety components and hardware for potential faults. If a fault is detected, the operation of the apparatus is halted and the screen reports the condition of the detected fault. 4.0.0 Fuses Over-current fuses are included in the motor circuit, as recommended by the manufacturer, and monitored by the controller. In the event a fuse has blown, one of three messages will appear: !! Fuse Error !! Check Left Fuse   !! Fuse Error !! Check Right Fuse !! Fuse Error !! Check Both Fuses Use a flat-blade (common) screwdriver to open the fuse holders and inspect the fuses Press the Apply button on the front panel to reset the error and return to normal operation. 29 4.0.1 Motor Type No two linear-actuator’s feedback signals are exactly identical. Likewise, there can be slight differences in the analog circuitry within the Incline Controller which processes these signals. Therefore, each linearactuator is programmed to a specific position: LEFT or RIGHT, and they are calibrated to their respective ports on the Incline Controller. As a result, all three components must never be exchanged with parts from another system to ensure proper operation and expected accuracy. In the event that the motors are not correctly detected, the following messages will appear: Motor Type Error Left Absent Motor Type Error Right Absent Motor Type Error Both Absent Motor Type Error Left Wrong Type Motor Type Error Right Wrong Type Motor Type Error Both Wrong Type     If a motor is “Absent”: Check the motor extension cables and Incline Controller port connections. If the “Left” or “Right” is the “Wrong Type”, most likely you have two “lefts” or “rights” from two separate incline apparatus’. Check the serial numbers on the linear-actuators against the Incline Controller. If both are the “Wrong Type”: Simply swap the motor or port connections with each other. Press the Apply button on the front panel to reset the error and return to normal operation. 4.0.2 Motor Feedback This can indicate a potentially serious error. If this occurs, it means the Incline controller has received an invalid signal from the feedback potentiometer of the linear-actuator. The potentiometer may have become worn and can no longer determine the actuator’s position. In the event of this error, one of three messages will appear: Feedback Error Left Motor   Feedback Error Right Motor Feedback Error Both Motors Open the cable connections. Clean any oxidation from the connector pins and remove debris from the receptacles. You can chemically clean the pins and receptacles with spray Contact Cleaner. Then reconnect. Press the Apply button to reset the error and return to normal operation. If the error remains, you may have to contact Columbus Instruments for service. 30 4.0.3 Positional Mismatch As two linear-actuators are used to move the apparatus, their positions must closely match. If one moves ahead or behind too much, then mechanical stress and damage may result within the Incline Apparatus. In the event of this error, controls are provided to manually move the linear-actuators back into alignment with each other.    The screen will display “Mismatch Error”. Press the Apply button to continue. The screen will display “Set Incline/Left to: ±xx.x° ±xx.x°”. Adjust the “INCLINE” knob so the angular degrees match. Press the Apply button to continue. The screen will display “Set Rate/Right to: ±xx.x° ±xx.x°”. Adjust the “RATE” knob so the angular degrees match. Press the Apply button to continue. Now the knobs are locked to the current position of the linear-actuators and the screen will display: Left ±xx.x°   4.1 Right ±xx.x° X Slowly adjust the desired knob, “INCLINE” for the left actuator and “RATE” for the right, until the angular degrees match. The “X” will switch to a “√”. Then press the Apply button to continue. The screen will display “Set Rate to: ±xx.x°/s ±xx.x°/s”. Adjust the “RATE” knob so the angular rates match. Press the Apply button to return to normal operation. Stop Mode When the mode switch is set to “STOP”, the electrical power to the linear-actuators is switched off and all mechanical motion should cease. The “INCLINE” and “RATE” knobs still adjust the desired set-points of the apparatus. 4.2 Run Mode When the mode switch is set to “RUN”, electrical power to the linear-actuators is restored. They will begin to move in the direction of inclination set by the “INCLINE” knob at the rate set by the “RATE” knob. Any adjustments to the inclination or rate will immediately take effect. 4.3 Step Mode When the mode is set to “STEP”, the “INCLINE” and “RATE” settings are not applied until the Apply button is pressed. Once pressed, the current settings are applied, and the apparatus will move to the new inclination at the new rate. While in motion, the “INCLINE” and “RATE” knobs may be adjusted with no effect on the applied settings. This allows the experimenter to apply an exercise profile manually by presetting the next inclination and then applying it at the specified time in lieu of computer control. 31 32 SECTION 5 COMPUTER-CONTROLLED EXPERIMENT 5.0 Treadmill Software Initialization From the desktop taskbar select: “Start >> All Programs >> Treadmill Software >> Treadmill Software”. Upon initialization, the software will automatically scan all of the available communication (COM) ports for existing devices and associated addresses. Once checked, all information windows will automatically close and leave you at the main window. 33 5.1 Device Scan To rescan the selected port for existing or new devices select “Tools >> Device Scan” from the main menu.    Click Scan Configured Devices , to check for expected devices. Click Scan for New Devices , to discover all connected devices. Click the Exit button complete the process and close the window. Based upon their unique address, devices will work together to control the faculties of a group:  Group 1: Treadmill Controller: (220), Shock Detection Controller: (230) and Incline Controller: (235).  Group 2: Treadmill Controller: (221), Shock Detection Controller: (231) and Incline Controller: (236).  Group 3: Treadmill Controller: (222), Shock Detection Controller: (232) and Incline Controller: (237).  Group 4: Treadmill Controller: (223), Shock Detection Controller: (233) and Incline Controller: (238).  Group 5: Treadmill Controller: (224), Shock Detection Controller: (234) and Incline Controller: (239).  Click Yes , to save the newly listed devices or No , to keep the previously configured devices. 34 5.2 Experiment Parameters Select “Experimet >> Run” from the main menu. You may want to “maximize” the Treadmill Software window to view all of the setting simultaneously. Refer to the “Exer 3/6 Treadmill, Treadmill Controller and Treadmill Software” manual for complete descriptions and procedures for performing an experiment. 35 5.2.0 Manual Tab The “Manual” tab lists the controls which select the experiment data collection and duration times as well as the treadmill, shock detection, and inclinometer settings for a single speed or acceleration experiment. When the “Profile Mode” is enabled, they indicate the current settings which are applied to the system. The following details their labels and descriptions:  Experiment Interval: the time that elapses between data collections. Duration: the total time of the experiment. A data collection is also performed at the end of the experiment should the integer multiple of the “Interval” not coincide with the “Duration”. A value of zero (0), will record intervals indefinitely until the user clicks Stop .  Treadmill Enable Profile Mode: Accel Step: Accel Int: Max Speed: S_Speed: Belt Speed: Shock Rep_Rate: Shock Intensity: This is used to apply various speeds and inclines within the same session. Refer to section “5.2.1 Profile Tab” for more details. The change in speed (meters/min) over the acceleration interval. The span of time (seconds) to apply the change in speed. Sets the terminal speed to accelerate towards. Sets the initial speed to accelerat from. Sliding indicator of the belt speed (0.0 – 102.3m/m). Selects the shock repetition rate. Records the shock intensity (must match the front panel setting).  Shock Detection Session Number: Number of Active Channels: Number of Visits to Grid: Number of Shocks: Sets the session number. Sets the active channels: Metabolic (1-4), Exer 3/6 (3 or 6) The maximum number of visits (detected by the shock repetition rate). The maximum number of shocks.  Inclinometer Range: Displays the allowable range of motion of the incline apparatus. Belt Angle: Sets the angle of inclination. Inc_Rate: Displays the rate of change of the inclination. The frame which groups the incline controls will be labeled with the type of incline apparatus detected:  Metabolic Mouse: -10.0° - +25.0°, Rate: 2.9°/s  Metabolic Rat: -10.0° - +25.0°, Rate: 2.1°/s  Exer 3/6: -10.0° - +25.0°, Rate: 1.6°/s Likewise, the Incline Rate is set to the apparatus’ maximum so it will move quickly move from one angle to the next and allow the calculation of work during the intervals more accurate. 36 5.2.1 Profile Tab The “Profile” tab is used to apply a set of parameters, sequentially in steps, throughout the experiment. Ideally, the sum of all of the “Period sec” entries should equal an integer multiple of the experiment “Duration”. The following details the control labels and descriptions: Step: The sequence number which logically progress: 1, 2, 3, etc.. Loop Profile: This is used to repeat the steps once all have finished. Start Speed m/m: The speed (in meters per minute) at the onset of the step. The first step, by default, begins at zero. The following steps begin at the previous step’s “End Speed”. End Speed m/m: The speed at the end of the step. Period sec: The duration (in seconds) of the step. Belt Angle: The desired angle of inclination at the onset and throughout the step. Shock: The status of the shock. “False” will disable the shock and halt the tabulation of “Time on Belt (TOB), Distance (M), Number of Shocks (NOS) and Number of Visits (NOV). “True” will enable the shock and resume the tabulations. Comment: Allows the user to indicate the purpose or comment about the step of the profile. At the onset of each step, the three parameters: Start Speed, End Speed and Period are used to calculate the acceleration rate for that step. Then the Treadmill Controller is commanded to change speed to the “End Speed” at that calculated acceleration rate. Concurrently, the Incline Controller is commanded to the Belt Angle and the Shock Detection Controller will apply or not, the shock and tabulation. 37 The Add Period will add another step to the profile. The Remove Period will delete the last step of the profile. The graph at the bottom pictorially indicates speed of the treadmill over time. 38 SECTION 6 MAINTENANCE 6.0 Cleaning For safety, switch off and unplug all of the equipment from the AC power. All of the parts of the incline controller and apparatus can be cleaned with a mild-soap solution. Do not immerse the linear-actuators or Incline Controller cabinet and try to prevent liquids from entering the components as this may damage the electronics inside. 39