Fein Optic Mi40 Inverted Microscope User`s

Transcript

Mi40 Microscope User’s Manual for Inverted Metallurgical Microscopes [email protected] Microscope Components: Metal Stage Trinocular Port Eyepieces Fixed Stage Halogen Bulb Vertical Adjustment Screw Halogen Bulb Horizontal Adjustment Screw Beam Splitter Field Diaphragm Centering Screw Coarse Tension Adjustment Power Switch Coarse Focus Page 1 Fine Focus [email protected] Microscope Components: Trinocular Port Carrying Handle Polarizer Diopter Adjustment Metallurgical Objectives Aperture Diaphragm Adjustment Field Diaphragm Adjustment Rheostat Control 360˚ Rotating Analyzer Coarse Focus Adjustment Fine Focus Adjustment Page 2 [email protected] Microscope Assembly: Follow the numbered diagram below when assembling the microscope. 6 4 5 7 1 2 3 11 10 9 12 8 Page 3 [email protected] Before Use: Do not shake or drop the microscope. Do not expose the microscope to direct sunlight or store in high temperature, damp or dusty areas. Make sure the workspace is horizontal. Indoor temperature should be between 41-104°F with a maximum relative humidity 80%. When moving the microscope, use one hand to hold the lower part of the observation tubes (1) and the other hand to hold the handle (2). Do NOT hold the microscope by the stage, focusing knob, head or light source when carrying it. When working, make sure the light source (3) has enough room for any heat to dissipate. Before replacing the fuse, make sure the power switch (4) is in the “O” (off ) position. Voltage range of 100~240V is supported. Make sure voltage is in this range. Only use the power cord supplied with the microscope. Microscope Maintenance: Wipe lenses gently with a soft tissue. Carefully wipe off fingerprints on the lens surface with a tissue moistened with a small amount of 3:7 mixture of alcohol and ether or dimethylbenzene, or a microscope cleaning solution. Do not use organic solution to wipe the surfaces of other components. If needed use neutral detergent. If the microscope becomes wet, power it off immediately and wipe all surfaces dry. Do not disassemble the microscope. When not in use, cover the microscope with the dust cover. Page 4 [email protected] Microscope Assembly: Installing the Bulb Loosen the set screw (1) with a coin or flathead screwdriver. Rotate about 45˚ clockwise and remove the bulb holder (2). Hold the bulb (3) with a glove or lens paper. Do not touch the bulb with your fingers. Insert the bulb pins (4) into the socket (5) making sure the bulb is vertical. Place the bulb holder (2) back in the housing and retighten the set screw (1). Bulb: 12v, 50w Halogen #R40POL-001. CAUTION: If replacing the bulb during or after operation, the bulb and housing will be very hot. Set the power switch to “O” off and remove the power cord. Allow some time for the bulb to cool. Assemble the Light Housing Loosen the set screw (1) with an M4 inner hex spanner. Insert the light housing (2) into the connector (3) and rotate so the top of the light housing (4) is horizontal. Retighten the set screw (1) with the M4 spanner. During operation leave enough room around the light housing for heat to dissipate. Install the Objectives Rotate the coarse focus knob (1) until the nospiece is in it’s lowest location. Install the objectives (2) into the microscope nosepiece from the lowest magnification to the highest in a clockwise direction from the left to right. Clean the objectives on a regular basis with lens tissue. When searching and focusing on a sample start with the lowest magnification 5x or 10x lens, then move up to the higher magnification. When changing magnification, twist the nosepiece until the next objective lens clicks into place. Page 5 [email protected] Microscope Assembly: Assemble the Mechanical Stage Attach the mechanical stage by lining up the set screws (1) on the mechanical stage with the screw holes on the microscope (2). Tighten the screws once the stage is attached. Assemble the Metal Stage Insert Insert the metal stage (1) onto the microscope. The stage hole (2) should be right above the objective lens. Assemble the Eyepieces Remove the eyetube covers (1) from the eyetubes. Insert the eyepiece (2) into the eyetube all the way. Insert the Ground Glass Filter Slider Remove one handle (2) from the ground glass filter slider (1) and insert the slider into the slot (3) on the illuminator connector panel. It should click into place. Reattach the handle (2) that was removed in order to slide the slider into the slot. Insert the Color Filter or Polarizer Insert the color filter slider or polarizer (1) into the slot on the illuminator connector panel (2). The slider should click into place. Page 6 [email protected] Microscope Assembly: Insert the 360˚ Rotatable Analyzer Insert the analyzer (1) facing up into the slot below the objective lenses (2). The analzyer should click into place. Connect the Power Supply Ensure the main switch is in the “O” off position. Insert one side of the wire (1) into the aviation plug socket (2) and lock it with the screw. Insert the other side of the wire (1) to the aviation socket (3). Insert the power cord (4) into the microscope body power socket (5). Insert the other end into the power outlet. Illumination Turn on the power supply and turn the power switch to “-” on. Adjust the rheostat control (1) clockwise to brighten the image and counterclockwise to dim the light. Using the bulb at a lower voltage will increase the life of the bulb. Adjusting a Sample Place the sample in the center of the stage (1). In order to secure the sample, use the holder (3) along with the attachable mechanical stage (2). The clip (4) will hold the sample holder in place. The mechanical stage can be adjusted with the X-Axis adjustment (5) or the Y-Axis adjustment (6) in order to move the sample to the desired location. Working range is 120mm x 80mm. Page 7 [email protected] Microscope Operation: Using the Beam Splitter Slide the beam splitter (1) to direct light 20% to the eyepieces and 80% to the trinocular port (camera). Slide the beam splitter the other direction to send light 100% to the eyepieces. Focusing Place the sample on the stage and move the 5x objective into the light path. The surface of the sample should be perpendicular to the objective. Adjust the right diopter adjustment ring to “0” and look through the right eyepiece. Adjust the coarse focus knob (1) and then the fine focus knob (2). Adjust the Focusing Tension If it is hard to turn the coarse focus knob or the sample falls out of focus (the stage drifts), solve the problem by adjusting the focus tension adjustment ring (1). To tighten the focus mechanism turn the tension ring clockwise. To loosen the tension turn it counterclockwise. Adjust the Diopters Turn the coarse focusing knob and then the fine focus knob to get a clear image while viewing through the left eyepiece only. Then look through the right eyepiece. If the image is unclear rotate the diopter adjustment ring (1). There is a +/-5 diopter on the ring. The number shown on the diopter (2) is your eye’s specific setting. Note the number that lines up with the mark on the side of the eyetube (3). Remember this number if multiple people are using the microscope. Adjust the Interpupillary Distance When using both eyepieces, hold the base of the prism and rotate them around the axis until there is only one field of view. The mark (1) on the side will line up with a number (2) that shows your specific interpupillary distance. Remember this number if multiple people are using the microscope. Interpupillary adjustment range = 50~75mm. Page 8 [email protected] Microscope Operation: Using the Eye Shields If the user wears glasses, turn the eye shields down (1) to prevent the glasses from touching the eyepiece and avoid damaging the glasses or the eyepieces. Open the eyesheilds (2) to prevent stray light from disturbing observation. Use the Ground Glass Filter When the ground glass filter slider (1) is pulled to the left it is used to center the filament. When it is pulled to the right it will help create uniform illumination. Typically this filter is set to the right in the engaged position. Use the Color Filter The color filter (1) can make the background more suitable and improve image reflection. There are four types of color filters that are typically used: blue, green, yellow and white. Adjust the Field Diaphragm By limiting the diameter of the beam of light entering the condenser, the field diaphragm can strengthen image contrast. When the image of the field diaphragm is on the edge of the field of view the clearest image is obtained. Adjust the field diaphragm rod (1) to minimize the field diaphragm. The image of the field diaphragm can be seen through the eyetube. Adjust the right and left centering screws (2) on the field diaphragm until the image is centered. Open the field diaphragm slowly. If the image is inscribed in the field of view, the field diaphragm has been centered properly. Open the field diaphragm slightly when using the microscope. Page 9 [email protected] Microscope Operation: Adjust the Aperture Diaphragm The aperture diaphragm decides the numerical aperture (NA) of the illumination. If the NA of the illumination matches the NA of the objective, the best resolution is obtained with increased contrast and depth of field. Adjusting the aperture diaphragm is the same as adjusting the field diaphragm. Adjust the size of the aperture diaphragm for comfortable viewing. Center the Light Filament Adjust the filament to the center of the field of view for even illumination. Shift the ground glass slider (1) out of the optical path until it clicks. Set the field diaphragm lever (4) and the aperture diaphragm lever (5) to the left. Place a white piece of paper on the stage. Rotate the nosepiece without an objective in it into the light path. Adjust the halogen bulb horizontal adjustment screw (2) and the vertical adjustment screw (3) to center the light. Adjust the focus lock handle (6) to get a clear filament image, then center it in the field of view. Pull the ground glass slider back into the light path and put a sample on the stage, adjusting the focus knob for a clear image. Observe through the eyepieces and once a clear, focused image is obtained, lock the focus lock handle (6). Adjust the field diaphragm (4) and aperture (5) for comfortable observation. Using the Simple Polarizing Kits The simple polarizing kit includes the polarizer (1) and the 360˚ rotating analyzer (2). When using the polarizer remove the color filter from the light path. Dialing the turntable on the 360˚ rotating analyzer can change the orthogonal states of the polarized light. When the turntable on the analyzer is set to “0” the analyzer and polarizer are in the orthogonal state. Replacing the Fuse Before replacing the fuse, make sure the power is switched to “O” off and unplug the microscope. Use a screwdriver to remove the fuse (1) from the fuse holder (2) and replace it with a new one. Fuse: 250V, 315A Page 10 [email protected] Microscope C-Mount & Objectives: Objective Type LWD Plan Achromat Semi APO Brightfield Part # / Magnification Numerical Aperture FMPLN5 / 5x FMPLN10 / 10x FMPLN20 / 20x FMPLN50 / 50x BF-SAPO-M5 / 5x BF-SAPO-M10 / 10x 0.15 0.30 0.45 0.55 0.15 0.30 0.50 0.80 0.90 BF-SAPO-M20 / 20x BF-SAPO-M50 / 50x BF-SAPO-M100 / 100x Working Distance 10.8mm 12.2mm 4mm 7.9mm 19.5mm 10.9mm 3.2mm 1.2mm 1mm Using the C-Mount Adapter Loosen the set screw (1) on the trinocular port and remove the dust cover (2). Remove the dust covers from the c-mount adapter (3) and place the c-mount on the trinocular port, tightening the set screw (1). Loosen the set screw (4) on the c-mount adapter to remove the c-mount (5). Connect the camera and then replace the c-mount adapter. Adjust the image through the eyepieces and then pull the beam splitter to direct light to the camera. If the camera image is not clear, adjust the c-mount focus (6) until the image is clear in both the eyepieces and the camera at the same time. Page 11 [email protected] Troubleshooting: Optical Troubleshooting Problem Light is bright, but field of view is dark. Side of the field of view is dark or uneven. Dust is observed in the field of view. Image is not clear. One side of field of view is dark or the image moves while focusing. Eyes fatigue quickly during use or the right field of view doesn’t match with the left. Cause Solution Field diaphragm is not large enough. Open the field diaphragm. Rheostat on light is turned down. Adjust the rheostat. Polarizer or analyzer blocking light. Remove polarizer/analyzer. Beam splitter is pulled out. Push the beam splitter in. Nosepiece is not clicked into position. Stain or dust has accumulated on the condenser, objective, eyepieces or light source. The filter, polarizer or analyzer is not in position. Beam splitter is not in correct position. Rotate nosepiece into place. Clean surfaces of condenser, objectives, eyepieces and light source. Remove / reinsert filters and sliders. Dust has accumulated on the specimen. Clean the sample. Dust is on the objective or eyepiece. Clean the objective and eyepieces. A cover slip is being used. Remove cover slip. The sample is not vertical to the objective. Adjust sample. Aperture diaphragm not open. Adjust aperture diaphragm. Stain or dust is on the lens. Clean eyepiece / objective. Beam splitter is not in correct position. Adjust beam splitter. Specimen is not fixed. Adjust sample on stage. Nosepiece is not clicked into position. Click nosepiece into place. Light is not centered properly. Interpupillary distance is not set properly. Center the light bulb. Adjust interpupillary distance. Diopter adjustment is not set properly. Adjust the diopters. Different eyepieces are being used in the left and right eyetube. Use the same eyepieces in each eyetube. Use the Fein Optic FPL-WF10x, FN22 Eyepieces. Page 12 Adjust the beam splitter. [email protected] Troubleshooting: Mechanical & Electrical Troubleshooting Problem Cause Solution Mechanical Troubleshooting: Coarse focusing knob is too tight. Tension knob is too tight. Loosen tension knob slightly. Stage drifts or falls. Tension knob is too loose. Tighten tension knob slightly. Electrical Troubleshooting: Problem Cause Solution Power is not turned on. Check power cable connection. Bulb is not installed properly. Check light connection. Bulb is burned out. Replace Bulb. Bulb burns out quickly. Incorrect bulb is being used. Replace bulb with part # R40POL-001. Field of view is not bright enough. Incorrect bulb is being used. Replace bulb with part # R40POL-001. Rheostat adjustment is turned down. Adjust the rheostat control. The connector pins or the wires for the bulb are not connected properly. Check wire connections and connector pins for the bulb. The bulb needs to be replaced. Replace bulb with part # R40POL-001. Light does not work. Bulb flickers or the brightness is not stable. Page 13 [email protected] Bulb Replacement: Replacement Bulb for Mi40, 12v 50w Halogen = R40POL-001 To replace the bulb on the Mi40, unscrew the top silver screw on the light housing as shown at right. The panel will open. Remove the panel. Depress the gold prongs as shown below and insert the bulb using a glove or lens tissue paper (do not touch the bulb). Once the bulb is inserted (note the red arrows for insertion point), release the prongs. Replace the panel in the light housing. Retighten the screw. Page 14 [email protected]