Transcript
HG Precentered Fiber Illuminator
(manual) (motorized)
HG Precentered Fiber Illuminator
Difficult lamp alignment is a thing of the past The Intensilight mercury-fiber illuminator employs a precentered, long-life lamp that requires no centering while allowing users to capture high-quality fluorescence images with uniform brightness.
C-HGFIE (motorized)
Greatly increased brightness for green spectrum
DC lighting for constant light intensity As DC (direct current) lighting is less influenced by frequency than AC (alternate current) lighting, DC provides constant, nonfluctuating light.
At wavelengths of around 450nm to 500nm, brightness is much higher than that of a conventional mercury lamp, making the lamp ideally suited to observation of green fluorescence such as FITC and GFP.
C-HGFI (manual)
Spectrum comparison
Typical AC lighting
DC lighting
100.0
80.0
The use of a precentered lamp and dedicated optical fibers eliminates the need for cumbersome centering and focusing operations, even after the lamp is replaced. Uniform brightness is always assured.
There are six levels of light intensity to choose from depending on the specimen. The shutter allows light to the specimen to be easily shut off without the power being turned off. Therefore, photobleaching can be reduced to a minimum when observing multi-stained specimens.
70.0
Relative intensity
Precentered lamp requires no alignment
Shutter and light intensity control
60.0
Relative intensity
Relative intensity
90.0
5
5
50.0 40.0
0
30.0
0.00
Time
80.00ms
0
0.00
20.0 10.0
Intensilight Mercury lamp 36 4 36 8 40 7 42 9 45 0 47 1 49 2 51 3 53 4 55 5 57 5 59 6 61 6 63 6 65 7 67 6 69 6 71 6 73 6 75 5 77 4 79 4
32 1 34 2
0.0
Wavelength (nm)
Motorized model C-HGFIE available Off-center
Precentered
Light intensity control (manual)
Shutter (manual)
2000-hour long-life lamp
Safety measures
The lamp lasts an average of 2000 hours, or 10 times longer than conventional mercury lamps. Therefore, replacement costs and microscope down time are greatly reduced.
When the lamp replacement cover is open or the optical fibers are not attached, the interlock automatically shuts the light off to protect the user from possible light exposure. Furthermore, when the lamp temperature sensor detects abnormally high temperatures, power is cut to protect the lamp.
The light intensity and shutter can be controlled from an optional dedicated remote controller or a PC that incorporates Nikon’s NIS-Elements imaging software. The light intensity and shutter can be programmed for each application and controlled in conjunction with the microscope and peripheral equipment. This enables automatic control during excitation light changeover and observation of multi-stained specimens. Remote controller
Reduced heat and electrical noise Dedicated optical fibers (1.5m, 3m) allow the light source to be placed away from the microscope, reducing heat and electrical noise on the microscope body. This is particularly suited to long-time fluorescence observation (time-lapse observation) of live cells. Configured with ECLIPSE TE2000-E
Time
80.00ms
Specifications
Dimensional diagram
Lamp
Ultrahigh pressure 130W mercury lamp
Lamp life
Average 2000 hours
ND (light intensity)
1 (100%), 2 (50%), 4 (25%), 8 (12%), 16 (6%), 32 (3%)
Shutter response
(Motorized) 100msec
Power supply
100-240VAC 50/60Hz
Dimensions (including protrusions)
(Manual) 110 (W) x 307 (D) x 278.5 (H)mm (Motorized) 110 (W) x 296 (D) x 278.5 (H)mm
Weight
Approx. 6kg
Optical fiber length
1.5m/3.0m
31
Unit: mm 18
258
110
278.5
25.5
90
7
C-HGFI (manual)
System diagram C-HGFI Intensilight (manual)
Epi-fluorescence attachment for the TE2000
C-HGFIA HG100W adapter
Epi-fluorescence attachment for TS100
C-HGFIF15 HG fiber 1.5m T1-HGFIA HG50W adapter
C-LHGFI HG lamp
Epi-fluorescence attachment for stereoscopic microscopes
C-HGFIF-C HG controller
P-FLA-HGFA adapter A
Digital imaging head Universal epi-fluorescence attachment C-HGFIB HG100 adapter B
Epi-fluorescence attachment for 50i/55i
C-HGFIF30 HG fiber 3.0m
Epi-fluorescence attachment for AZ100
PC
C-HGFIE Intensilight (motorized)
C-HGFA fiber adapter
NIS-Elements imaging software
Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. November 2006 ©2006 NIKON CORPORATION WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
* Monitor images are simulated. Company names and product names appearing in this brochure are their registered trademarks or trademarks.
NIKON CORPORATION Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0005, Japan phone: +81-44-223-2167 fax: +81-44-223-2182 http://www.nikon-instruments.jp/eng/
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Printed in Japan (0611-00)T
Code No. 2CE-OOOO-1
This brochure is printed on recycled paper made from 40% used material.
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