Transcript
HPV Extraction Kit User’s Guide
Table of Contents
Overview·····························································································································1
Operation Principle·······································································································1
Contents·····························································································································3
Warning and Precautions··························································································3
Experimental Methods·································································································4
Storage································································································································6
Explanation of Symbols······························································································6
HPV Extraction Kit “HPV Extraction Kit is IVD”
Overview The HPV Extraction Kit is an automatic extraction system for the isolation of high quality DNA from Cervical swab. This Extraction Kit is used with the Nextractor® to provide high-yield and quality DNA from your sample. Please refer to the manual for details about the Nextractor®.
Operation Principle Transfer the specimen into the sample well, insert the plate into the Nextractor® ,
and start the program. The first step of extraction is 『lysis and binding DNA to magnetic bead』, in which
the specimen is mixed with lysis solution and magnetic beads. In this step, DNA is extracted from the specimen, and then bound to magnetic beads. The second step of extraction is 『DNA WashingⅠ/ WashingⅡ』. In this step, inhibitors affecting the PCR reaction are removed by washing solution. The third step of extraction is 『DNA Elution』. In this step, DNA attached to the magnetic beads is dissolved into the elution buffer.
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Contents 1. HPV Extraction Kit Cat. No
TEST
Contents
Specimen
Extraction plate (HPV) (96well) x4ea HP1102
96 Strip x12ea Cervical swab Extraction plate (HPV) (32well) x6ea
HP1111
48 Strip x6ea
Warning and Precautions 1. All samples must be treated as potential biohazards. 2. Wear appropriate protective eyewear, clothing, and gloves. 3. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water 4. Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (i.e. fume hood). 5. All work should be conducted in properly equipped facilities using the appropriate safety equipment (i.e. physical containment devices). 6. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially infectious materials.
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Experimental Methods The HPV Extraction Kit should be used with the Nextractor®(Gyeongin 14-66). ※ All extractions must be processed in 2~35℃. ※ Our extraction kit must be stored in 2~35℃. If the extraction kit has been stored above 35℃, the result would be affected. 1. Sample collection and storage 1.1 Sample collection Collect samples in a sterile container. 1.2 Sample storage 1) Store at 4°C for up to one week. 2) Store at –70°C for long-term storage. 2. Specimen Preparation 2.1 Cervical swab sample 1) Vortex Cervical swab sample for 10~30 seconds. ※ If samples are stored frozen, submerge them in a water bath at 25–37°C until completely thawed, then place on ice until further use. 3. Experimental methods HPV DNA extraction from cervical swab sample (Cat No : HP1102, HP1111) 1) Determine the number of samples to be tested and carefully remove the sealing tape from the cartridge of the HPV Extraction Kit. 2) Dispense 250 μl of cervical/virginal swab into the sample wells(96-well: 1st, 5th, and 9th wells; 32-well: 1st well) of the HPV Extraction plate. 3) Insert the plate and strip to the Nextractor®and press Universal for extraction. 4) Once the operation is completed, remove the plate and take a 5 μl DNA for HPV detection.
- If there is the magnetic bead in the Elution Buffer after extraction, take the
DNA/RNA after placing the ‘well plate’ on top of the provided ‘magnetic board’ so that the ‘magnetic beads’ are fixed to the magnetic board.
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*How to start program
① Touch
② Touch
③ Touch
button
button
button to start extraction
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Storage HPV Extraction Kit should be stored at 2℃~35℃.
Kit
Contents
HPV Extraction Kit
Extraction plate(HPV) Strip
Storage Temperature 2℃~35℃
shelf life 1 year
Explanation of Symbols
Don’t reuse
Please refer to the manual
Lot Number
Catalog Number
In-vitro Diagnostics product
Manufacturing date
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Product Name
HPV Extraction Kit
Category
Nucleic acid extraction Kit
License Number
Seoul 14-360
Publish date
2014.11.13
Final publish date
2015.12.09 11F, Asan Institute for Life Sciences 88 Olympic-Ro 43-Gil, Songpa-Gu, Seoul, Korea,
Manufacturer
138-736
Tel. +82 2-3010-8670 Fax. +82 2-3010-8671
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