Illumination Handbook For Leica Stereomicroscopes

Transcript

Illumination Handbook for Leica Stereomicroscopes V 1.0 1 The Leica SMS Illumination Handbook Table of Contents 1 The Leica SMS Illumination Handbook 4 Fluorescence Table of Contents 2 Let there be light! 3 Usage of this handbook 5 1.1 Importance of Illumination Systems 6 1.2 When do I need which Illumination? 7 2 Incident Light Solutions 4.1 Introduction 64 4.2 Leica Fluorescence Module 65 4.3 Leica MZ16 F / FA 68 5 Leica LED Solutions 5.1 Introduction 70 5.2 LED1000 Solutions 71 5.3 Leica VisiLED 72 5.4 Leica LED2000 75 5.4 Leica  LED2500 76 2.1 LED1000 Spotlight 10 2.2 Leica L2 Universal Lightguide 11 2.3 Goosnecks 12 2.4 Combination Lightguides 17 2.5 Ringlights 20 6.1 Leica L2 78 2.6 Near Vertical Illumination 35 6.2 Photonic Coldlight Sources 79 2.7 Coaxial Illumination 38 6.3 Schott Coldlight 82 2.8 Cross Section Converter 45 3 Transmitted Light Solutions 6 Cold Light Solutions 7 Accessories 7.1 Adapter to mount fibre light guides 88 3.1 Transmitted Light Stages 49 7.2 Ringlight Adapter for Objectives 89 3.2 Transmitted Light Bases 54 7.3 Adapters for swing arm stands 90 3.3 Polarisation 59 7.4 Color filter for CLS 92 7.5 Base Plate for CLS 94 7.6 Segmentation attachment for continuous slit ring lights (CLS) 95 8 System Diagrams and Catalog References 8.1 Leica LED1000 97 8.2 Leica VisiLED 99 8.3 Fluorescent Ringlight 101 8.4 Leica L2 102 8.5 Photonic Cold Light Sources 103 8.6 Schott Cold Light Sources 106 8.7 Leica Transmitted Light Bases 109 Panorama of Lake Constance. The background shows the Leica SMS Demo room with all available illumination switched on. Let there be light! Welcome to the first edition of the Leica SMS Illumination Handbook. This catalogue provides you with a concise overview of all illumination related products and accessories that are available in the stereomicroscopy range of Leica Microsystems. We hope you find this useful in your daily work.  Panorama of Lake Constance. The background shows the Leica SMS Demo room with all available illumination switched on. Let there be light! The first version of the Illumination Handbook concentrates on the product portfolio. In the next edition, we aim to integrate more application images. If you have any interesting images that you would like to share, then please send them, along with information on the type of illumination used to: [email protected] This will help you and your colleagues find the best illumination for each application.  1 Usage of this handbook 1 To ensure that this handbook is as easy to use as possible, you’ll find that the navigation and basic information is consistently placed on each page. 2 1 / 2 Product name Following Pages In instances where there is too much product information to fit on a single page, this indicator will show you the number of following pages. Simply turn to the next page with the buttons shown in the navigation-bar (4) 2 Product Picture For a quick overview, each page will include a current product picture 3 Info-Box 3 This box contains the most important facts. Along with order numbers and related products, you will find links to a system-diagram and a catalogue reference, both of which will rapidly accelerate the speed of your search for vital product information. 4 QUICK INFO Order Number...........................30 111 481 System Diagram....................... click here Catalog Reference................... click here Navigation Bar (f.l.t.r.) • back-button: go back one page • link to top of chapter • link to the table of contents • forward-button: have a look at the next page 4  1.1 Importance of Illumination Systems In Stereomicroscopy, the correct illumination is the key to informative investigation and inspection. The better the object is illuminated, the more details become visible, and thus the more reliable the inspection and the better the photographic documentation. The illumination system is not just an accessory, it is a very important part of the complete system. Only the correct illumination system will ensure that a customer will see the structures they really want to. It is very important, that the illumination system matches perfectly to the desired application. Depending on the final magnification, especially the highest magnification, it is necessary to choose the proper performance (brightness) of the illumination. This illumination handbook gives you an overview of all Leica SMS illumination solutions. The handbook gives you ideas on when to offer which illumination and gives you some tips on available accessories. Goal of the handbook: No quotation without an illumination !!!  1.2 When and which Illumination do I need? 1.2.1 Incident light Incident light is needed for all kinds of non-transparent specimens. Depending on the texture of a specimen and the goal of the results, there is a huge list of incident illumination systems available. Ringlights Ringlights for example, provide a very bright and homogeneous illumination. For special applications, a polarization kit for ringlights is available. Fluorescent ringlight To reduce reflections on high reflective specimens we recommend to use a fluorescent ring light. Coaxial illuminations For wavers and other highly reflective surfaces, we recommend the use of coaxial illumination, which delivers more information than any other incident illumination system. Gooseneck For low working distance objectives, and for higher contrast purposes, gooseneck fibre light guides are recommended. They can be adjusted at different angles to the specimen to deliver a variety of contrasts. Near vertical illumination If the specimen has deep depressions or holes that have to be illuminated, we recommend the use of near vertical illumination. The near vertical illumination also provides a shadow free view of the specimen  1.2 When do I need which Illumination? 1.2.2 Transmitted light Transmitted light is needed for all kinds of transparent specimens Standard transmitted brightfield illumination Standard transmitted brightfield illumination is used for all kinds of transparent specimens with high contrast and sufficient colour information. Darkfield illumination Darkfield observation is an illumination from the side. The main difference between this and oblique illumination is that no light will be reflected through the objective. Image taken with brightfield TL illumination. Oblique transmitted illumination This illumination technique is used for specimens that are virtually transparent and colorless. Due to the oblique position of the illumination a greater contrast and visual clarity of the specimen can be achieved. Image taken with oblique TL illumination.  2 Incident Light Solutions 2.1 LED1000 Spotlight 2.5.6 Combined Ringlight for BF / DF 28 2.5.7 Fluorescence Ringlight 29 2.2 Leica L2 Universal Lightguide 2.5.8 Schott Ringlight: VisiLED 30 2.5.9 Schott Standard Ringlight 31 2.3 Goosnecks 2.5.9 Schott Darkfield Ringlight 32 2.5.10 DF-Ringlight for Planapo 2.0× 33 2.5.11 Schott Maxi-Ringlight 34 2.3.1 Leica L2 Gooseneck 13 2.3.2 Photonic Gooseneck 14 2.3.3 Schott Gooseneck 16 2.4 Combination Lightguides 2.4.1 Photonic Combination Lightguides 18 2.4.2 Schott Combination Lightguides 19 2.5 Ringlights Introduction 21 2.5.1 Leica LED1000 22 2.5.2 Leica L2 Ringlight 23 2.5.3 Photonic Standard Ringlight 24 2.5.3 Photonic Standard Ringlight 25 2.5.4 Maxi Ringlight 26 2.5.5 Ringlight for revolving nosepiece 27 2.6 Near Vertical Illumination 2.6.1 Solutions for M-Series 36 2.6.2 Solutions for S-Series 37 2.7 Coaxial Illumination 2.7.1 Coaxial Illumination for M-Series 39 2.7.2 Coaxial Illumination for S-Series 40 2.7.3 Coaxial Illumination for Z-Series 41 2.7.4 Usable Zoomrange of Coax. Illum. 42 2.8 Cross Section Converter 2.8.1 Photonic Solution 46 2.8.2 Schott Solution 47  2.1 LED1000 Spotlight Leica LED1000 Spotlight The LED spotlight contains 19 LED’s and can be attached directly to the microscope and positioned as required by means of a Gooseneck. The gooseneck can also be attached to the base plate. Available accessories • Filters for spotlight • Gooseneck arms for spotlight • Base plate for Gooseneck arm One control unit can handle • One ringlight • One or two spotlights • One ringlight and one spotlight Pros • • Daylight quality at 5000K Long service lifetime Cons • limited brightness level for lower magnifications only • no optimum mounting possibilities QUICK INFO Order Number.......................... 30 220 001 System Diagram....................... click here Catalog Reference................... click here 10 2.2 Leica L2 Universal Lightguide leica L2 Universal Lightguide The universal light guide can be mounted directly on to the focusing arm from the back of the instrument. With an easy mount, you can have light from either side or from both or optionally insert it in to the transmitted light base for transmitted light. Incident Light • with integrated focusing lens • 700mm length • daylight filter mountable Part Numbers 10 446 392 Universal light guide 10 447 152 Double universal light guide 10 447 317 Universal light guide, 1000mm Known Limitations Incident light with universal light guide mount 10 447 009 By mounting the universal light guide directly to the microscope carrier, the additional supplementary lens will result in a shadow within the FOV. With a universal light guide mount (10 447 009), this problem can be resolved. For the S8APO, this mount is generally recommended. QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here Universal light guide mount combined with transmitted light using the double universal light guide 11 2.3 Goosnecks Goosenecks are often used when customers need a freely configurable incident illumination system. The advantage of goosenecks is that they maintain their position without the need for mounts. Goosenecks also allow different contrasts to be achieved by using a variety of angles for the illumination. Bringing the gooseneck very close to the specimen at high magnifications results in increased brightness. Goosenecks are a good solution for configurations where a low working objective is in use (i.e. 1.6× or 2.0×). For Petridishes, which are typically used for samples, it is sometimes necessary to have incident light. Goosenecks are often used for this purpose. 12 2.3.1 Leica L2 Gooseneck Leica L2 Gooseneck Without focusing lens Focusing lens With focusing lens QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here Without daylight filter Daylight filter With daylight filter Part Numbers Accessories 10 446 388 Single Gooseneck, 500 mm 10 446 389 Double Gooseneck, 500 mm 10 446 391 Focusing lens 10 447 055 Day light filter for focusing lens 13 2.3.2 Photonic Gooseneck Photonic Gooseneck There are different goosenecks available for various application requirements: 30 130 011 30 130 012 30 130 021 30 130 022 30 130 031 30 130 422 Gooseneck 1arm 4.5 mm / 600 mm Gooseneck 1arm 3 mm / 500 mm Gooseneck 2arms 3 mm / 500 mm Gooseneck 2arms 4.5 mm / 600 mm Gooseneck 3arms 4.5 mm / 600 mm Gooseneck 2-arms 4.5 mm / 600 mm ESD Accessories 30 161 101 30 161 102 30 161 110 30 161 113 30 161 114 30 161 115 30 161 116 30 161 107 30 161 108 30 161 109 Filter/Lens-holder Focusing lens, 20 mm diameter Focusing lens, 26 mm diameter Filter blue, 20 mm diameter Filter green, 20 mm diameter Filter yellow, 20 mm diameter Filter red, 20 mm diameter Filter daylight, 20 mm diameter Filter polarization, 20 mm diameter Diffusor, 20 mm diameter QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here 14 2.3.2 Photonic Gooseneck Q A What’s the difference in light output between the 3 mm and 4.5 mm goosenecks, other than the fact that the active bundle is smaller/larger? The active diameters of the light guides are responsible for the transmission of light. The diameter is not the best dimension to show the differences. It is much better to look at the area dimensions in square millimetres. The 3 mm light guide has an area of 7.09 square millimetres. The 4.5 mm gooseneck light guide has 15.9 square millimetres. If you are using a double gooseneck, there are further differences. The diameter on the side of the cold light source is 6 mm and 9 mm respectively. The 2-arm 3 mm gooseneck has 7.09 square millimetres on each arm and 28.27 to the cold light source. In comparison to this, the 2-arm 4.5 mm gooseneck light guide has 15.9 square millimetres on each arm and 63.62 square millimetres to the cold light source. Photonic Gooseneck However, further factors influence the brightness. All light guides are made from the same glass fibres with the same diameters. The bulbs in the cold light source have 30% tolerances in brightness. As you can see, a lot of factors influence the brightness. Q A I have a customer who requires replacement lens caps for a twin gooseneck on a CLS 150 (4.5mm). Can you help please? Article number for the cap 30161202 Focusing attachment, 4.5 – 5 mm QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here Article Number for 3 mm goosenecks 30161201 Focusing attachment, 3 mm Most cold light sources have an illumination angle so that it is possible to illuminate up to 10 mm active diameter (=78.54 square millimetres). The illumination is uniform across the 78.54 square millimetres. It is clear that a light guide with an active dimension of 63.62 square millimetres can take 125% more light from the light source than a 28.27 square millimetres light guide. 15 2.3.3 Schott Gooseneck Schott Gooseneck Available Goosenecks Accessories 31 154 101 Gooseneck light guides 1 arm 4.5 mm / 600 mm 31 154 202 Gooseneck light guides 2 arms 4.5 mm / 600 mm 31 154 302 Gooseneck light guides 3 arms 4.5 mm / 600 mm 10 446 391 31 158 200 31 158 205 Focusing lens f. light guides up to 5 mm (Gooseneck or flexible light guides) Focusing lens with filter set (including blue, red, green and yellow filters) Polarization filter for focusing lens (5 mm) QUICK INFO Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here 16 2.4 Combination Lightguides As the name suggests, this fibre light guide is a combination between a double gooseneck and a flexible fibre light. The gooseneck can either be mounted directly on the focus column or the flexible fibre light guide can be mounted from the cold light source. The distinct advantage of this is that it is not necessary to move the cold light source close to the base of the system. Goosenecks also provide good illumination options for swing arm stands. Please refer to the special swing arm stand adapters section. 17 2.4.1 Photonic Combination Lightguides Photonic Comb. lightguides Combination light guides (Gooseneck with 2 arms, light guide length 750 / 1000 / 1500 / 2000 mm) 30 140 001 30 140 002 30 140 003 30 140 004 Combination light guide 2 arms, 4.5 mm / 750 mm Combination light guide 2 arms, 4.5 mm / 1000 mm Combination light guide 2 arms, 4.5 mm / 1500 mm Combination light guide 2 arms, 4.5 mm / 2000 mm Explanation • • 4.5mm is the active arm diameter of the light guide 750/ 1000/ 1500/ 2000 mm are the available lengths of the lightguides Mounting 10 445 654 Clamp for combination light guide is needed to mount the light guide to the M-Series focus columns. QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here 18 2.4.2 Schott Combination Lightguides Schott Comb. lightguides 31154402 Combination light guides, 2 arm 600 mm Gooseneck with 2 arms 600 mm for illumination, 1 arm 850 mm for connection with the cold light source Mounting 10445654 Clamp for combination light guide is needed to mount the light guide to the M-Series focus columns. QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here 19 2.5 Ringlights Introduction LED1000 Leica L2 Ringlight Photonic Ringlights • • • • Standard Ringlights Maxi Ringlight Ringlight for revolving nosepiece Fluorescent Ringlight Schott Ringlights • • • • VisiLED Ringlight Standard Ringlights Darkfield Ringlight for PlanApo 2.0x Objective Maxi-Ringlight 20 Introduction Ringlights can be used for all kinds of applications where homogeneity is needed and are mounted on the objectives. This gives the advantage that illuminations are easily reproduced. • • • • • high brightness due to top specification homogeneity reproducable light situation mounted on objectives accessories (diffusor, polarization) Image of a circuit board taken with a Leica S6 D and Gooseneck Illumination. Image of a circuit board taken with a Leica S6 D and the LED1000 Ringlight. 21 2.5.1 Leica LED1000 Leica LED1000 The LED ringlight contains 40 LEDs and provides an exceptionally bright and homogeneous illumination of an object. It is designed for microscopes with an objective diameter of 66 mm. The ringlight comes with a 66/58 mm adapter that can be mounted on smaller objectives. • contains 40 LEDs • bright and homogenious illumination • designed for objectives with diameter of 66 mm (with adapter also for 58 mm) Image of a screw taken with LED1000 Image of a Clockwork taken with LED1000 QUICK INFO Order Number.......................... 30 210 002 System Diagram....................... click here Catalog Reference................... click here 22 2.5.2 Leica L2 Ringlight L2 Ringlight In most industrial and laboratory environments, bench space is vital. The Leica L2 is not only the smallest (125mm x 110mm x 150mm) and lightest cold light source (0.5kg), it is also the only one that can be attached directly to the stereomicroscope stand. • • • • • • 6-segment ringlight for 58 mm diameter objectives length: 750 mm shadowfree homogeneous bright Adjustment of light intensity (3 steps) QUICK INFO Order Number.......................... 30 210 002 System Diagram....................... click here Catalog Reference................... click here L2 Ringlight mounted on a Leica S4 E. The Lightguide can be easily fixed by guiding it through the microscope holder. 23 2.5.3 Photonic Standard Ringlight 1/2 Photonic standard ringlight • 6-segment ringlight • continuous ringlight • for 66 mm diameter objectives Partnumbers for 6-segment ringlights: • 30 120 101 Ringlight 6 segment RL6-66/750 • 30 120 102 Ringlight 6 segment RL6-66/1000 • 30 120 103 Ringlight 6 segment RL6-66/7,6/1500 • 30 120 109 Ringlight 6 segment RL6-66/2000 Partnumbers for continuous ringlight: • 30 120 201 Ringlight RL-66/750 • 30 120 202 Ringlight RL-66/1000 • 30 120 203 Ringlight RL6-66/1500 • 30 120 204 Ringlight RL-66/2000 • 30 120 401 Ringlight RL-66/750 ESD Accessories QUICK INFO Order Number.......................... 30 210 002 System Diagram....................... click here Catalog Reference................... click here Polarization for ringlight guides • 30 121 101 Polarizing filter-set for RL66 • 30 121 102 Polarizing filter for RL66 • 30 121 103 Analyser filter for RL66 Diffusor for ringlight guides • 30 121 201 Diffusor filter for RL66 Protection for ringlight guide • 30 121 401 Protection glass 24 2.5.3 Photonic Standard Ringlight Polarization Set The Ring light can be used together with the polarization set 30 121 101. The polarization set helps to reduce high reflections from PCB boards. Image taken without polarization filter Image taken with polarization filter Segmentation attachment (for continuous slit ringlights) The advantage of the segmentation attachment is that customers can reproduce light conditions and optimize the light and shadow effect in the specimen level. It is possible to cover a part of the slit ringlight (between 120° and 240°, click stops on 150°, 180°, 210°) and it is possible to rotate this light cover 360° till finding the correct position to illuminate the specimen. The primary applications for this item are in the forensics and in the quality control. The focusing attachment must be removed before the segment attachment can be fixed. The segmentation attachment can be used for the following ringlights: 2/2 Accessories Photonic standard ringlight Continuous ringlights: • 30 120 201 • 30 120 202 • 30 120 203 • 30 120 204 Order Number 30 121 202 Segmentation attachment for RL66 Note: The attachment does not fit to the old version of ringlight, please contact [email protected] for details. QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here Spare part: 30 122 105 Focusing attachment variable cover size 360° revolvable focusing attachment must be removed 25 2.5.4 Maxi Ringlight Maxi Ringlight The Maxi Ringlight is a very good solution when used in combination with the oblique observation attachment for inspecting PCB boards in the semiconductor industry. Partnumber 30 120 301 Maxi Ringlight The adapter can be mounted in two positions so that the flexible light guide can be positioned along each side of the stand to the CLS cold light source at the back of the microscope. The mountable focus arm can only be used in the position that it is shown in picture 4. Mount adapter for maxi ringlight 30 123 303 Maxi Ringlight Adapter The adapter is delivered together with two screws; (A) is to be fixed on the mountable focus arm. The screws fit in to the two holes (B) as shown below. QUICK INFO The Maxi ringlight fits to the following microscope carriers: • • • • • Order Number.......................... 30 120 301 System Diagram....................... click here Catalog Reference................... click here 10 447 151 (old) 10 447 422 10 447 423 10 447 424 10 447 425 26 2.5.5 Ringlight for revolving nosepiece R.L. FOR REVOLVING NOSEPIECE 1 2 The ringlight has a levelling (see picture 1) on each side (beside the coming out of the flexible part of the fibre light guide) so that the ringlight can be attached to the 1.0x objective when the objective is in the front position of the revolving nose piece. With these two levellings the ring illuminator can be fixed in two different positions so that the flexible part of the fibre light guide tube can be run along of each side of the microscope to the CLS cold light source in the back of the microscope. See picture 2 and 3. Before switching the revolving nosepiece into the other position (the 2.0× objective will be in the front position) the ringlight has to be removed completely. 3 TIP QUICK INFO Order Number.......................... 30 120 205 System Diagram....................... click here Catalog Reference................... click here The Ring light can be used together with the polarization set 30 121 101! 27 2.5.6 Combined Ringlight for BF / DF combined ringlight bf/df The new combined ringlight can be used for brightfield and darkfield illuminations and can be easily switched between the two as required. Switching is accomplished by opening the locking screw and moving the ringlight up and down the objective (please see the arrow in the picture to the right). Darkfield illumination is the closest position to the specimen. • • Inner diameter of the ringlight is 70 mm. The ringlight is delivered with an adapter 70/66 mm Suitable for the following objectives: • • • • planapo objective 2.0x (without adapter) planapo objective 1.6x (adapter is included) planapo objective 5x (with adapter 30 123 135) achromat objective 1.5x and 2.0x HP (with adapter 30 123 134) For other objectives with longer working distances (66 mm diameter) an extension is required. Please refer to the price list. Due to the short working distance of the ringlight to the specimen, a gliding stage is an absolute must. The new ringlight can be used with all CLS cold light sources from Leica. Polarization and the segmentation attachment cannot be used with this ringlight. • • Inner diameter of the ringlight is 70 mm. The ringlight is delivered with an adapter 70 / 66 mm Tip With a Motor Focus, it is possible to move the microscope upwards and downwards quickly to find the right focus position. This is particularly useful in cases where the specimen needs to be changed regularly as it saves valuable time and money. QUICK INFO Order Number.......................... 30 120 407 System Diagram....................... click here Catalog Reference................... click here 28 2.5.7 Fluorescence Ringlight Fluorescence ringlight The fluorescence ringlight is specifically designed for illumination tasks where a uniform and even light is required, such as in the case of a PCB inspection (solder joint). The ringlight below shows fewer reflections, reducing eye strain for the operator. Features • • • • • • • • Image taken without polarization filter Image taken with polarization filter • • • • Constant colour temperature 5.500 K Ripple- and flicker-free illumination by highfrequent current supply (25 kHz) 2-step-brightness control without variation of colour temperature Increased lamp-life by a new smooth lamp ignition 360° – shadow free illumination Unique ESD-safe model Sound- and vibration-free operation Universal mounting system for objectives up to 66 mm diameter Easy and tool-free lamp change Independent stand-alone operation possible “Cold light” Segments can be controlled in different preset modes for optimum contrasts. QUICK INFO Order Number.......................... 30 310 001 System Diagram....................... click here Catalog Reference................... click here Available accessories • • • • ESD grid High power version UV version Useful working distance between 35 mm to 135 mm 29 2.5.8 Schott Ringlight: VisiLED Schott VisiLED Ringlight Features • • • • • Easily adaptable to microscope objectives by means of thumb screw and clamp ring Homogenous illumination Best white quality: typically 6,000 K Long operating life: typically 10,000 h by actively controlling the LED’s temperature Segments controllable in different preset modes for optimum contrasting Ringlights and Accessories Description Part Number Working Distance (WD) Light Flux Max. Illluminance Inner ∅ Outer ∅ LED Brightfield Ringlight „S40–25“ 31 400 100 55–110 mm (2.17‘‘ – 4.33‘‘) 50 lm 35 klx (75 mm) (2.95‘‘) 66 mm (2.60‘‘) 114 mm (4.49‘‘) LED Brightfield Ringlight „S80–25“ 31 400 225 25–50 mm (0.98‘‘ – 1.97‘‘) 100 lm 120 klx (30 mm) (1.18‘‘) 66 mm (2.60‘‘) 114 mm (4.49‘‘) LED Brightfield Ringlight „S80–55“ 31 400 255 55–135 mm (2.17‘‘ – 5.32‘‘) 100 lm 75 klx (70 mm) (2.76‘‘) 66 mm (2.60‘‘) 114 mm (4.49‘‘) QUICK INFO Order Number............................... see left System Diagram....................... click here Catalog Reference................... click here 30 2.5.9 Schott Standard Ringlight SCHOTT standard Ringlight Partnumbers for 4-segment ringlights Compatibility 31 157 401 4-point ringlight ∅ 30 mm inner diameter 31 157 402 4-point ringlight ∅ 66 mm inner diameter M-Series S-Series Z-Series Partnumbers for continuous ringlight 31 157 410 Continuous ringlight 58mm inner diameter – best working distance between 50 and 120 mm. When using the Leica MZ75 in combination with an achromat objective, two adapter rings (10 446 300) are required! (MZ75 one) see „Adapters for Objectives“ Objective Adapter 31 157 450 Objective Adapter 31 157 450 Tip The ringlight can be used together with the polarization set 31 158 430 and 31 158 440. The polarization set helps to reduce high reflexes at PCB boards. 31 157 420 Continuous ringlight (symbol - please leave as is, just can’t find it in word) 66 mm inner diameter – best working distance between 50 and 120 mm QUICK INFO Only for KL2500 LCD Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here 31 257 420 Continous Ringlight ∅ = 66 mm Image taken without polarization filter Image taken with polarization filter Accessories Polarization for ringlight guides 31 158 430 Polarization Filterset for continous ringlight ∅ 66 mm 31 158 440 Polarization Filterset for continous ringlight ∅ 58 mm 31 2.5.9 Schott Darkfield Ringlight Darkfield ringlight What is Darkfield? Features The light source is at the side of the objective. When rays impinge on a mirror, they are reflected at the same angle and do not enter the objective. The field of view remains dark. Non-planar surfaces are seen in their natural colours. Non-planar surfaces are seen in its natural colours. Two inclined lamps should be used for photomicrography. • • • • • Easily adaptable to microscope objectives by means of thumb screw and adapter rings Homogenous illumination Best white quality: typically 6,000 K Long operating life: typically 10,000 h by actively controlling the LED chip temperature Segments controllable in different preset modes for optimum contrasting Application example Standard Ringlight QUICK INFO Order Number..........................31 400 300 System Diagram....................... click here Catalog Reference................... click here Darkfield Ringlight 32 2.5.10 DF-Ringlight for Planapo 2.0× DF-ringlight 2.0× We have modified the incident darkfield illumination system to a new diameter of 70 mm to be used together with the 2.0x PlanApo objective. Together with this, an adapter is delivered to mount the ring light on to the 1.6x PlanApo objective (diameter 66mm). Due to the short working distance of the ringlight to the specimen, a gliding stage is an absolute must. • • The dark field ringlight is suitable for the cold light sources KL1500 LCD and KL2500 LCD. For all other objectives, an extension is required. Please refer to the price list. QUICK INFO Order Number.......................... 31 157 406 System Diagram....................... click here Catalog Reference................... click here RELATED PRODUCTS Adapters for Objectives 33 2.5.11 Schott Maxi-Ringlight Schott Maxi Ringlight The Maxi-Ringlight from Schott can be mounted directly on the objective. As the Maxi-Ringlight and the image rotator use the same interface, they cannot be mounted at the same time. However, with an extension ring (part number 35 000 024) you can avoid this problem and mount the Schott Maxi-Ringlight and the image rotator at the same time. 31 157 445 Objective adapter for maxi ringlight (in combination with achromat objective 1.0×, 58 mm) QUICK INFO Order Number.......................... 31 157 440 System Diagram....................... click here Catalog Reference................... click here 34 2.6 Near Vertical Illumination The light rays enter the specimen at almost a 90° angle. Thus, you can illuminate the bottom of depressions or holes. Since the light rays move downwards almost vertically, it allows you to work in a shadow free environment (micro-dissecting). Ringlight The almost vertical beam of light penetrates recesses and enables boreholes and cavities to be observed. Disturbing shadows which may be thrown by tools are avoided during work. Near Vertical Illumination 35 2.6.1 Solutions for M-Series Solutions for M-Series The vertical illuminator is used with interchangeable objectives of 100 mm, 150 mm, 175 mm or 200 mm focal lengths. The f=200 mm objective fully illuminates even the largest field of view (66 mm) and provides an incidence angle of approximately 5°. Please order the appropriate intermediate rings to match the instrument. For the Leica MZ75 two adapter rings are required (10 446 300). For the Leica MZ95 one adapter ring are required (10 446 300). For the Leica MZ12.5 and MZ16, one adapter ring is required (10 446 300) along with a second adapter ring (10 446 303). Suitable objectives for near vertical: 10 411 597* Achromatic objective f= 100 mm 10 441 787* Achromatic objective f= 150 mm 10 431 692* Achromatic objective f= 175 mm 10 382 162* Achromatic objective f= 200 mm 10 457 297* Achromatic objective f= 225 mm 10 407 743* Achromatic objective f= 250 mm 10 457 298* Achromatic objective f= 275 mm 10 382 168* Achromatic objective f= 300 mm 10 431 693* Achromatic objective f= 350 mm 10 382 172* Achromatic objective f= 400 mm * Objectives for vertical illuminator Suitable cold light sources KL1500 LCD and CLS150X / MED Suitable fibre light guides 30 150 702 31 155 302 Flex light guide 1arm TVC 9 mm / 750 mm (Photonic) Flexible light guide for vertical illumination (Schott) Tip Together with the AX-carrier, it is easier to inspect deep holes. QUICK INFO Near vertical with stereoview Order Number.......................... 10 445 198 System Diagram....................... click here Catalog Reference................... click here Near vertical with AX carrier The near vertical illumination with achromatic objectives larger than f=200mm does not illuminate the complete FOV. (max 60mm) 36 2.6.2 Solutions for S-Series Solutions for S-Series The S-Series solution uses a different approach to the M-Series. The illumination is mounted between the instrument and the objective, reducing the working distance by 15 mm. • • For 58 mm diameter Use single fibre light guide 10 446 386 This solution can be easily adapted to the Z-line. Tip If you use near vertical illumination with a supplementary lens (0.5×, 0.63×, 0.75×) you have longer working distances and you need more brightness than the L2 can provide on its own. In such instances, an adapter (30 117 701) can be used alongside the L2 fibre light guides with a CLS50/100/150 cold light source which provides more brightness. QUICK INFO Order Number.......................... 10 446 372 System Diagram....................... click here Catalog Reference................... click here 37 2.7 Coaxial Illumination The light source is located above so that rays fall directly on to the specimen. A mirror surface will appear bright. Coaxial incident light is used to reveal the structures of flat, highly reflective objects such as wafers, integrated circuits, liquid-crystal displays and metal sections, by using interference colors. In using this method of illumination, the light rays are reflected directly into the two observation beam paths of the stereomicroscope. The light beams shine through the objective onto the reflecting surface of the object and are reflected back into the objective at the same angle. Coaxial incident illumination must be close to the optic axis. A quarter-wave plate is required when the microscope carrier AX is used in the vertical position. Specimen Wafer / Bright Field With ringlight Wafer / Dark Field Coaxial Illumination Coaxial Illumination 38 2.7.1 Coaxial Illumination for M-Series Solutions for M-Series Coaxial incident illuminator With this illumination, flat, reflecting object surfaces become visible. Magnifying optics 1.5x. Suitable cold light sources KL1500 LCD and CLS150X/MED Suitable fibre light guides 30 150 702 31 155 302 Flex light guide 1arm TVC 9 mm / 750 mm (Photonic) Flexible light guide for vertical illumination (Schott) QUICK INFO If you order the intermediate ring (10 446 300) for the MZ75 and MZ95 with a 1.0× planachromatic objective, then the largest fields of view will be fully illuminated. Please order a fibre-optic light guide (active diameter f = 10 mm, end diameter f = 13 mm) and a light source. Order Number.......................... 10 446 180 System Diagram....................... click here Catalog Reference................... click here 39 2.7.2 Coaxial Illumination for S-Series Solutions for S-Series Flat, highly reflective surfaces, such as wafers, integrated circuits and polished metal sections, can be seen well in coaxial incident light by virtue of the differing interference colours. In this illumination technique, the light beams are mirrored along the observation beam paths of the stereomicroscope. Key features • • • 58 mm interface for direct mounting to supplementary lenses A grey filter (neutral absorber) can be inserted for two different brightness levels Reduces working distance by 50 mm The lever on the right-hand side is used to swing a neutral absorber in and out. With the neutral absorber you can reduce the brightness without changing the color temperature. Limitations With the S8 APO, the working distance is very limited. (75 mm – 50 mm = 25 mm) • • Reduces working distance by 50 mm Insertable gray filter for two different brightness levels With the coaxial illuminator you need the double flexible light guide 10 446 387. QUICK INFO Order Number.......................... 10 446 373 System Diagram....................... click here Catalog Reference................... click here 40 2.7.3 Coaxial Illumination for Z-Series Solutions for Z-Series Key Points Recommended outfit for coaxial illumination • Compact housing • Built-in polarising filters to eliminate stray reflections. • • Needed quarter wave plate: 10 447 207 The Planapo 0.8× objective is specially designed for the coaxial illumination. Usable zoom range: 1.0 – 9.2×. Suitable cold light sources and fiber light guides 30 111 350 CLS 150X/MED (230 V-240 V) 30 111 360 CLS 150X/MED (100 V-120 V) 30 150 714 Flex light guide 1arm, act. Ø 6 mm, 1000 mm 31 150 200 31 150 201 31 250 200 31 250 201 31 155 401 Cold light source KL 1500 LCD, 230 V Cold light source KL 1500 LCD, 120 V Cold light source KL 2500 LCD, 230 V Cold light source KL 2500 LCD, 120 V Light guide, HL, TL BFDF base and coaxial illuminator Leica Z16 APO Planapo 0.8× Other objectives can be used in a limited zoom range • • Planapo 1.0× : usable Zoom range from 2.0 – 9.2× Planapo 2.0× : usable Zoom range from 3.2 – 9.2× QUICK INFO Order Number...........................10 447 170 System Diagram....................... click here Catalog Reference................... click here 41 2.7.4 Usable Zoom Range of Coax. Illum. Due to the optical design of the Leica Zoom line, there are limitations in using the coaxial illumination in conjunction with different objectives. Vignetting or pronounced shading is visible at certain zoom positions. At the edge of the field of view, only part of the light is reflected back to the objective. This is especially critical at low magnifications, but also occurs at medium zoom positions. In conditions, where the entire field is illuminated and no vignetting is visible, we can speak of nearly telecentric conditions. Our main competitors are avoiding the limitations of coaxial illumination by applying an illumination between the zoom body and the objective or just above the last lens of the zoom optics. In contrast to our competitors we are offering true coaxial illumination that uses the entire zoom as illumination optics. The advantages of our solution • • Higher intensity at higher magnificationsmore homogeneous intensity distribution throughout the zoom range higher contrast especially at higher magnifications 42 2.7.4 Usable Zoom Range of Coax. Illum. Important note Although the data shown in the graphs does not look very promising (with the exception of the 0.8x PlanApo illustration), one has to consider that the camera often attached to such systems will not pick up the entire field of view. The graphs show the results at 21 mm FOV with a Leica DFC280 and 0.5× C-Mount on an AS-tube giving the equivalent of about an 18mm FOV. An A Z16 Apo A with a 1× PlanApo will make vignetting barely visible. With a Leica DFC320 and a 0.8× C-Mount, the 2× PlanApo is able to produce homogeneous illuminated images at zoom factors above 2×. The Graphs to the right show the limitation of each single objective in conjunction with the Zoom body. 43 2.7.4 Coaxial Illumination with 5× Objective Q A Can I use coaxial illumination with a 5× Planapo on a Z-Line Instrument? Yes, under the following conditions: A Lambda/4 Plate for 5x PlanApo and HR-Objectives needs to be used. Since the introduction of the 5x PlanApo, an objective has been included in the range offering a resolution that was previously only available for compound microscopes. Up until now, this objective could only be used for fluorescence or transmitted light. However, our R&D colleagues have found a way to apply this objective (along with other HR-objectives) with coaxial illumination for use in many standard industrial applications. The Lambda Plate is built into an intermediate piece that is mounted between the objective and the optics carrier. To avoid reflections, the Lambda plate should be inclined by 4°. Benefits • • • Full usage of the 5x PlanApo within Industry No loss of working distance when using coaxial illumination (2x/ 5x PlanApo and HR-Objectives) Easy to use with no moving parts to avoid errors in operation To achieve best results in coaxial, the orientation of the lambda/4 has to be 45° to the orientation of the polariser and analyser of the coaxial illuminator. To achieve this, you can slightly open the alan screws on the coaxial and on the Z16 and slightly rotate the coaxial until you see the best contrast in the eyepieces (usually the brightest image). Limitations The use of the Z-Line in coaxial illumination with higher magnification objectives has some vignetting at certain zoom positions. Please refer to the Z-Line news on the SMS Business Web. These limitations are also applicable to the Lambda/4 plate. For the 5× PlanApo, the zoom range starts at above 2.5×. For the HR-Objectives (for the 10x and 20x Mitutoyu), the range begins at about 3.5× and finishes at approximately 7.5x. Anything above this will not provide any improvements to the magnification. The 2× PlanApo can be used with the new Lambda/4 Plate. However, please be aware that vignetting is visable in low zoom ranges and at high zoom positions when used with the HR-objectives. Please consider using a 0.8× C-Mount (even with ½ “ cameras), to remove some of the vignetting on the camera chip. For visual observation, 16x eyepieces may also be an alternative. Part Number: 10 447 460 44 2.8 Cross Section Converter Cross section converters are used to increase the surface contrast of very flat specimens. Most people know the difference between using a gooseneck illumination with or without a focusing lens. When used with the focusing lens, it is possible to concentrate the light directly on to a small and specific area. We now have a new attachment in our portfolio for the goosenecks and the flexible light guides (10 mm mount). The slit illumination attachment provides a very thin slit light (1-2 mm × 35 mm) producing a very flat angle similar to the dark field illumination. The difference is that a dark field ringlight is a circular illumination. With goosenecks or flexible light guides, the user can decide from which direction(s) and in which angle(s) the illumination should be positioned. A glass rod in front of the illuminator is used for focusing the light. Autograph illuminated with cross section converter Application This item is primarily used in quality control i.e. when flat surfaces or coatings need to be inspected or within forensics. This lighting is ideal for viewing structures, scratches or any marked surfaces. Even when examining documents, the new slit light attachment shows more detail. More examples • • • Passport control Paper Industry Signature inspection Autograph illuminated with ringlight 45 2.8.1 Photonic Solution Photonic Cross Section Conv. The Photonic cross section converter (see picture to the right) includes a flexible light guide and a cross section part as one unit. The lens is adjustable to achieve different angels in which the light illuminates the specimen and can be fixed with the two red screws. Mountings other than the cast foot, shown in the photo below, are also available (i.e. column or microscope clamps). The most versatile system would use the cast foot and the articulated arm. Goosenecks can be used as well. All flexible light guides are available for the CLS cold light sources with the exception of the TVC light guides. Alternative If you already own a flexible light guide or a Gooseneck you can also use the following product as an alternative to the cross section converter (see picture to the right): Mounting other than the cast foot, shown in the photo below, are also available (i.e. column or microscope clamps). The most versatile system would use the cast foot and the articulated arm. Goosenecks can be used as well. 30 170 121 Section Converter for Gooseneck QUICK INFO The slit light illuminator is very easy to use. The following items are required for the flexible light guides: 30 157 102 Cast foot 30 157 101 Articulated arm 30 157 103 Clamp 30 xxx xxx Any Flexible light guide Order Number...........................30 170 112 System Diagram....................... click here Catalog Reference................... click here Flexible light guide with cross section converter 46 2.8.2 Schott Solution Schott Cross Section Conv. The Schott cross section converter is available in four slit widths to fit all kinds of specimen sizes. The lens is adjustable to achieve different angles in which the light illuminates the specimen and can be fixed with two black screws. 31 160 100 31 160 110 31 160 120 31 260 130 Cross section transf. slit 50 × 1.2 mm Cross section transf. slit 100 × 0.6 mm Cross section transf. slit 200 × 0.3 mm Cross section transf. slit 300 × 0.3 mm, KL2500 LCD QUICK INFO Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here 47 3 Transmitted Light Solutions 3.1 Transmitted Light Stages 3.1.1 Leica L2 Transmitted Light Stage 49 3.1.2 Leica LED1000 Transmitted Light Stage 50 3.1.3 Photonic Transmitted Light Stage 51 3.1.4 Transmitted Stage Plate for Darkfield 52 3.2 Transmitted Light Bases 3.2.1 Transmitted Light Bases (S-Series) 54 3.2.2 Transmitted Light Bases TL ST 55 3.2.3 Transmitted Light Bases TL BFDF 56 3.2.3 Transmitted Light Base TL RC™ 57 3.2.4 Transmitted Light Base TL RCI™ 58 3.3 Polarization 48 3.1.1 Leica L2 Transmitted Light Stage l2 Transmitted light Leica offers a solution for nearly any kind of transparent specimen. The following section presents illumination solutions for different price/performance ranges and the different product lines. • Fits to 120 mm interface • Active light diameter: 50 mm • Modular item for future growth • Height of the stage is 15 mm The transmitted light stages are modular items which are ideal for customers looking for simple and very affordable transmitted light solutions. The illumination itself is also homogeneous. The main disadvantage is the height of the stage (L2) and the LED1000 cable. • Fits to flexible light guide 10 446 386 • Very affordable solution Needed light guide 10 446 392 Universal light guide QUICK INFO Order Number........................... 10 447 038 System Diagram....................... click here Catalog Reference................... click here 49 3.1.2 Leica LED1000 Transmitted Light Stage LED1000 Transmitted light This transmitted light illuminator can be integrated in with a stereomicroscope stand and features uniform daylight-quality illuminations, long life LEDs and a compact design. The brightness of the transmitted light can be infinitely regulated using the control unit. The control unit has two plug sockets for illumination systems, one plug socket for the 24 V input from the power supply. Even the control unit can be powered with a dry battery instead of the power supply. The power supply is equipped with a mains power plug (IEC320) and with a long low voltage connecting cable for the control unit. The power supply is suitable for voltages of 100 – 240V, has 50/60 Hz and is CE, UL and CSA approved. The power consumption is 15 VA. • 22 white LEDs • Carrier plate PVC white, Ø 120.5 x 5 mm • Illuminated surface Ø 55 mm • Colour temperature 5,000 K Needed Accessory 30 210 001 Control unit with power supply QUICK INFO Order Number........................... 30 240 001 System Diagram....................... click here Catalog Reference................... click here 50 3.1.3 Photonic Transmitted Light Stage Photonic Transmitted light The surface of this stage is a frosted multiple layer glass plate with a diameter of 95 mm. The transmitted light stage fits into the incident light base instead of the black/white table plate. The weight is sufficient for stable positioning on a table (e.g. in using with a swing arm stand) and additionally the contact surface to the table is coated with a anti skid surface. The transmitted light stage must be connected with the cold light source through a 2 arm flexible light guide. All other 2 arm flexible light guides from the CLS program are suitable. The above mentioned fibre light guides are only a selection of the most used fibre light guides. • Fits to all incident light bases with with 120 mm • Stage plate interface. • Fits to all CLS cold light sources. • Illuminated circle of 60 mm • Even and uniform illumination of the glass surface • Compact and solid housing • Two light guide connectors • Simple installation and handling • Maximum colour temperature is 3200°K A complete outfit contains Tip: 30 241 001 Transmitted light stage QUICK INFO The flexible light guide can also be used with holders for incident light. For this, the following items are required: 10 445 654 Column clamp 30 157 101 Articulated support arm (2x) 30 157 105 Adapter M6/M8 (2x) 30 157 103 Holder for flexible light guides (2x) Additionally focusing lenses can be used. plus Order Number........................... 30 241 001 System Diagram....................... click here Catalog Reference................... click here 30 150 211 Flexible light guide, 2 arms 3 mm or 30 150 221 Flexible light guide, 2 arms 5 mm (recommended) or 30 150 231 Flexible light guide, 2 arms 8 mm and a CLS cold light source. 51 3.1.4 Transmitted Stage Plate for Darkfield Transmitted stage plate DF The light source is situated beneath the plane of the specimen. However, direct rays are blocked by a central stop and rays which travel more laterally are reflected by a system of mirrors. Reflected rays traverse the specimen plane at such a shallow angle that they do not enter the objective and the field of view remains dark. Reflected rays which are refracted at interfaces within the specimen may enter the objective, producing a bright image on the dark background. • Fits to all incident light bases with with 120 mm • Easily adaptable to microscope objectives by means of thumb screw and adapter rings • Homogenous illumination • Best white quality: typically 6,000 K • Long operating life: typically 10,000 h by actively controlling the LED chip temperature • Segments controllable in different preset modes for optimum contrasting All other 2 arm flexible light guides from the CLS program are suitable. The above mentioned fibre light guides are only a selection of those that are most used. QUICK INFO A new transmitted darkfield light stage is available for the LED Order Number........................... 31 400 360 System Diagram....................... click here Catalog Reference................... click here illumination system, “VisiLED”. Switching between brightfield and darkfield is not possible as it is exclusively designed for darkfield illumination. In cases where the customer wants to switch between both Brightfield Illumination Darkfield Illumination illumination methods, they must use our transmitted light base, TL BFDF (10 446 351). 52 3.1.4 Transmitted Stage Plate for Darkfield 2/2 Transmitted stage plate DF The darkfield illumination system requires a controller and a power supply. For standard use i.e. for brightness control only, the MC750 controller with power supply is sufficient for darkfield illuminations. The MC750 basic controller has one connecting plug for one illuminator and a rotary knob to control the brightness. A temperature control is integrated to prevent the illuminator overheating. The MC1500 also includes methods for controlling brightness and heating but provides multiple functions so that two illuminators can be adjusted separately allowing the customer to use an additional illumination system, such as an incident ringlight, if required. Functions included within the MC1500 mean that predefined segments are switchable in ½ circle, ¼ circle, 2/4 circle and 4/8 circle. These segments can be rotated by hand or automated at a predefined speed. The speed is fixed for occasions when it is not being controlled by software. SDK software is provided for user programming. Demonstration software is also delivered as part of the package so that customers can intuitively control the following functions. • Brightness intensity • The use of predefined segments or the ability to create unique segments for customized illuminations • Settings available for controlling rotation direction and the intensity and pulse rate, which can be saved and recalled easily. The integrated memory means that an unlimited number of settings can be saved and recalled. The illumination is ripple free and the power supply is suitable for all voltages (100 – 230 V) although a suitable mains cable is needed. An optional foot switch is available for the MC1500 controller to switch between the saved settings. QUICK INFO Order Number........................... 31 400 360 System Diagram....................... click here Catalog Reference................... click here For other items, please refer to the price list. – A flash/strobe function is also integrated. This can be controlled by hand, by an external trigger or through software. The MC1500 controller has a RS232 interface and a USN adapter is optionally available. 53 3.2.1 Transmitted Light Bases (S-Series) Transmitted Light S-Series The S-Series transmitted light base (10 446 341) can be easily mounted on to the incident light base (10 446 340). This is a huge benefit as the customer can upgrade their existing StereoZoom configuration with a transmitted light base as and when required. The base has a mirror with clear and frosted glass that can be rotated. This mirror can also be moved to obtain oblique or single sided darkfield illuminations. • • • • • • Increases the height of the configuration by approximately 68 mm Modular item Easily mounted without the need for screws Rotatable mirror Clear and frosted glass Adjustable mirror for oblique and single sided darkfield illumination Compatible fibre light guide: 10 446 392 Universal light guide 10 447 152 Double universal light guide 10 447 317 Universal light guide, 1000mm QUICK INFO Order Number........................... 10 446 341 System Diagram....................... click here Catalog Reference................... click here 54 3.2.2 Transmitted Light Bases TL ST TL ST Variable light guide provides full control Work in comfort Operating the Leica TL ST transmitted light stand is easy. Simply set the brightness with a potentiometer. The mirror, which guides light through the specimen at different angles, can glide across the horizontal plane. The tilt angle of the mirror is then automatically aligned. In this way the user has full control of the transmitted light without having to spend time looking for the best transmitted light angle. Leica’s new halogen lamp is particularly efficient. With a capacity of only 20 W, it reaches the light utilization of a 35 W lamp. The heating effect is noticeably reduced, and temperature-sensitive specimens especially benefit from the lower wattage. A large glass plate protects the stand from any fluids, should a spillage occur. While a specimen is analyzed under the stereomicroscope, the other specimens can remain on the stand for quick changeover if need be. A large glass plate protects the stand from any fluids should a spill occur. Daylight filter reproduces natural color-tone Leica offers a daylight filter accessory for the standard transmitted light stand. The highly coated glass raises the color temperature and cuts off the infrared and UV spectrum of the halogen lamp. This provides a spectrum similar to daylight. QUICK INFO Order Number........................... 10 446 350 System Diagram....................... click here Catalog Reference................... click here Change the lamps in a matter of seconds The new, easy-to-use lamp changing mechanism enables quick replacement of a halogen lamp. After a cooling down phase, the user can access the lamp holder directly by removing two screws on the heat sink. 55 3.2.3 Transmitted Light Bases TL BFDF TL BFDF Continuous adjustable brightfield and darkfield changeover between Leica’s brightfield transmitted light is available for studying stained amplitude specimens with sufficient contrast. The new, high performance TL BFDF transmitted light stand offers versatile options. Light rays can be deflected through the specimen from steep to flat, and a high or low degree of diffusion can be selected. For example, if the light rays are completely deflected vertically through the specimen, an exact brightfield with maximum brightness is created. The specimen appears with full contrast and in natural color on a bright, homogenous background. However, if the light is deflected through the specimen at an oblique angle, this makes it easier to view semi-transparent specimens such as foraminifera and fish eggs. If the light is deflected through the specimen at an oblique angle, however, this makes it easier to view semi-transparent specimens such as foraminifera and fish eggs. The flatter the light rays are deflected into the specimens, the darker the background appears. Contours, fine edges, and structures are brightly and clearly emphasized by diffraction of the light on a dark background. Effective vibration dissipation The vibration-dissipating feet featured on the TL BFDF minimize disturbances during delicate work. External vibrations that influence the stand (impact sound, fan for external light sources etc.), and any direct disturbances on the stand itself are better dampened than with conventional stand feet. QUICK INFO Section through fingertip Section through fingertip Brightfield Darkfield Order Number........................... 10 446 351 System Diagram....................... click here Catalog Reference................... click here SlideOn™ design provides easy stage changeovers When purchasing a new Leica stereomicroscope stand, if the standard stage is ordered instead of the new XY stage, the standard stage (with Leica’s SlideOn™ design) can be quickly replaced by the new XY stage as and when is needed. 56 3.2.3 Transmitted Light Base TL RC™ TL RC™ Contrast method for clear, transparent specimens Constant color temperature Rottermann-Contrast™ is a partial illumination technique that shows changes of the refractive index as differences in brightness. Phase structures then typically appear as spatial, relief-type images like hills in positive relief contrast and as indentations in inverted relief contrast. The two integrated diaphragms that create the relief effect can be individually positioned from fully open to fully closed. The relief effect can also be regulated from slight to strong by precisely tilting the path-folding mirror with the rotary button on the left side of the stand. This technique offers many variable views for extracting the maximum possible amount of information from every specimen. Color temperature and brightness are controlled separately with Leica’s high-performance stands. Using the CCIC function, the brightness is set completely independently from the color temperature – and the required color impression remains exactly the same when the illumination intensity changes. The potentiometer for the color temperature also has an electronically controlled shutter. If the stand is switched on again after a work break, it automatically returns to the previous settings. Three available color filter slots The TL RC™ and TL RCI™ each feature three slots for color filters. These slots can be used for filters that are available as accessories, i.e. neutral gray, fluorescence filter BG38, and UV filter, or fitted with other filters as the user wishes. QUICK INFO Order Number........................... 10 447 390 System Diagram....................... click here Catalog Reference................... click here 57 3.2.4 Transmitted Light Base TL RCI™ TL RCI™ Perfect co-ordination with automated stereomicroscopes Leica’s TL RCI™ transmitted light stand features two USB ports and two CAN bus interfaces. The brightness of the light source can be controlled via a USB mouse. Combined with motorized Leica stereomicroscopes and macroscopes, motorized focus, and Leica Application Suite (LAS) software, the user has full computer control over the zoom level, focusing, color temperature, brightness, and shutter of the illumination. Many different settings can be stored and automated with the LAS software. Choice of an integrated or external light source The high-performance stands of the TL series are available in two designs: with integrated halogen illumination or with a connection for external cold light sources. If a user already has a cold light source, it can be used on the new stand. In addition, the new Leica CLS150 LS cold light source enables the internal shutter to be controlled directly via a PC. Accessories • Footswitch: Leica’s new footswitch, controlled by a potentiometer, features a CAN-Bus interface. As a result, the two rockers can be used for any microscope function, such as motorized focus, zoom control, and filter changing (i.e. Leica MZ16 FA). More details available in the system diagram QUICK INFO Order Number........................... 10 446 352 System Diagram....................... click here Catalog Reference................... click here 58 3.3 Polarization What is polarized light? If vibrations are generated in a loosely hanging rope, these will usually be propagated in a certain plane. Electromagnetic waves, too, vibrate in certain planes. If the electric vector of a light wave vibrates in one plane only, the light is called linearly polarized. Natural light, as emitted by all conventional light sources, is composed of an infinite number of linearly polarized waves which vibrate in different planes perpendicular to the direction of propagation. Unaided, the human eye is not able to distinguish between natural and polarized light. Polarized light is generated with birefringent materials. These have the characteristic of being able to split natural light into two linearly polarized waves vibrating perpendicular to each other and propagating in this material at different velocities. Polarizers used to be made of calcite prisms. Today, di-chroic foils are used which absorb one of the two perpendicularly polarized vibrations. Similar components are used as analyzers to detect the polarized light. If the vibration directions of the polarizer and the analyzer coincide, the polarized light is allowed to pass through the analyzer. However, if the vibration directions are at right angles to one another, the light is absorbed and the microscope field of view appears dark. Double-refracting material such as many organic and inorganic crystals (including minerals), bones, polymers, glass and liquid-crystal displays, can be studied on a transmitted-light stand equipped with a polarizing set. You can choose between the polarizer on the glass stage plate or the convenient centrable rotatable polarizing stage with mechanical stage and sensitive tint plate. Each of these sets should be combined with an analyzer in a rotatable mount. Each of these sets is to be combined with an analyzer in rotatable mount. Image of a plastics defect, taken with transmitted light stage and polarization. 59 3.3 Polarization The Sensitive Tint plate in the polarising stage is a Lambda plate (one of the numerous names for the same thing like Full Wave Plate, Gypsum Plate, etc.). It is used for highlighting small phase differences in birefringent material (like plastic foils, thin sections of rocks, glass, etc.). The use of this plate is similar to that on the compound microscopes. So by applying an additional phase difference (the „lambda“ of the plate or 546 nm of phase difference) it is easier to determine the actual phase difference in the sample. This is due to the fact, that the human eye is more sensitive to color changes than to brightness changes. 2/4 10 446 302 10 382 130 10 361 719 10 315 306 10 367 929 10 446 228 Rotatable polarization stage ∅ 120 mm with polarizer and clear glass stage plate. Attachable mechanical stage for rotatable polarization stage Sensitive-tint plate for rotatable polarization stage Analyzer in rotatable mount for achromat Analyzer in rotatable mount for planachromatic and plan-apochromatic Glass stage plate with polarizer ∅ 120 mm for transmitted light stands With the aid of an eyepiece graticule with crosshair, the rotatable Polstage can be centred so that features remain in the field of view and extinction angles can be measured. The range of movement of the mechanical stage is 76 mm x 28 mm. 60 3.3 Polarization Notes on the use of Leica polarization accessories Polarization accessories serve to find out information about double-refracting substances. Many crystals and fibres have this property, as do plastics and glass under strain. The optical contrast of such specimens is simultaneously enhanced. Normal light vibrates in all directions at right angles to its trajectory. If a polarising foil (e.g. 10 446 228, 10 315 306 or 10 367 929) is placed in its path, only components vibrating parallel to the polarization direction of the foil can pass through, and the emergent light is said to be plane-polarised. A crystal is termed pleochroic if it shows change in quality or quantity of colour when rotated in polarised light; there is otherwise little difference between the image of a thin piece of double-refracting material in ordinary light and in planepolarised light. However, if two polarizing foils are placed at right angles to one another, no light can pass through because the plane-polarized light passed by the first foil, is blocked by the second. The first foil is termed the polariser (10 446 228) and the second is the analyser (10 315 306 or 10 367 929). 3/4 its speed coincides with that of the ordinary ray only in one or two directions (the optic axis or axes); in all other directions a path difference builds up between the two rays. This path difference does not produce an interference colour, because the planes of polarization of the two components are at rightangles to one another. If a double-refracting substance is placed between crossed polars (i.e. polariser + analyser at right angles) the net effect is that the ordinary ray and the extraordinary ray which emerge from the analyser are plane-polarised in the same direction. Consequently, the path difference between them which results from their different speeds within the specimen becomes evident as an interference colour (in this context more usually termed polarization colour). The production of such colours is the basic purpose of the polarising microscope. The polarization colour seen depends not only on the specific double-refracting power of the substance (its birefringence), but also on: • the direction of observation (because of the direction- dependent difference between the two speeds within the crystal) • the thickness of the specimen (the longer the traverse, the greater the final lag between the ordinary and extraordinary ray) Double-refracting substances split light into two components. One component, the ordinary ray, moves within the substance at a constant speed independent of its direction. The other, the extraordinary ray, is direction-dependent and 61 3.3 Polarization If the double-refracting substance is rotated between crossed polars it becomes totally dark (extinction position) and attains maximum brightness (45° position) four times in a full revolution. The extinction position is compared with the simultaneous position of the edges of crystals in order to measure the extinction angle - this helps to identify the crystal. The 45° position is used to study the polarization colours. The centring rotating polarization stage (10 446 301) is therefore useful. This stage has to be centrable, because otherwise features would drift out of the field of view during the rotation. It is used in conjunction with a crosshair graticule (10 376 120) in the eyepiece. The crosshairs help with the centring procedure and serve as a reference for the measurement of extinction angles. The stage 10 446 301 has an incorporated swing-in polariser and so requires only an analyser (10 315 306 or 10 367 929) and no additional polariser. The polarization colour seen in the 45° position can be identified from a Michel-Levy chart. As the sequence of colours tends to be repeated in increasing orders, the order to which a given colour belongs may be uncertain. This problem can be resolved by slowly introducing a quartz wedge in a certain orientation between the polars so that its steadily-increasing influence progressively reduces the path difference to zero. The original colour is then obtained by comparing the changing colours with those on the chart. Such a wedge is used with classical petrological microscopes, but is too small for stereomicroscopes or macroscopes. It is a type of compensator. 4/4 Another type of compensator that can be used with stereomicroscopes, is the sensitive tint plate (10 361 719). This is also known as the first-order red plate because, if used without the specimen and rotated to the 45° position, it produces the purplish-red colour at the end of the first order on the MichelLevy chart. The colour is sensitive in that a path difference change of only a few nanometres will change it to bright red (decrease) or to bright blue (increase). Consequently it can be used to detect very weak birefringence, because weaklybirefringent specimens alone between crossed polars would produce only a grey shade. It can also be used to determine whether an indeterminate pale grey polarization colour represents a low or an extremely high path difference (very high order polarization colours are also pale). Its addition or subtraction of one order to many orders will produce negligible change, but its combination with a low grey polarization colour will produce a brilliant first-order colour instead. The sensitive-tint plate can also be used to produce spectacular colour effects in the photography of many types of specimen, in particular for plant cells with crystalline inclusions and for insects with chitinous parts. 62 4 Fluorescence 4.1 Introduction 4.2 Leica Fluorescence Module 4.3 Leica MZ16 F / FA 63 4.1 Introduction What is fluorescence? In fluorescence microscopy, the contrast of selected object structures, utilizes the fact that some materials when irradiated with ultraviolet, blue or green light, will emit light of a longer wavelength than that of the exciting light (Stokes’ law). Fluorescence cannot be explained with the physical terminology of geometrical and wave optics used so far. However, quantum optics teaches us to imagine light as being composed of many energy quanta, the photons, which are absorbed upon penetration into certain materials. Their energy is then used to transpose electrons of certain atoms into higher states of energy, although these are unstable. When the electrons return to a lower stable energy state, part of the released energy is emitted in the form of a photon and part converted into other forms of energy. Fluorescence substances are illuminated when irradiated with short-wave light. Stereo fluorescence is a non-destructive, observational technique for research (molecular cell biology, biochemistry, molecular, pharmacology, biology etc.) and for metal, electronic and paper industries and those within criminology. Leica MZ16 FA with EL6000 Illumination, TL RCI™ Transmitted Light Base and IsoPro™ Cross-Stage 64 4.2 Leica Fluorescence Module Leica Fluorescence Module There are six different fluorescence modules available. The combinations for GFP and GFP Plus include special filters for GFP applications. The GFP Plus filter combinatin is optimized for GFP Plus (a mutant, optimized GFP Protein and shifted towards the red spectrum) The excitation filter is placed on a filter slide in the Fluorescence Module. Barrier filters and dichroic mirrors are permanently mounted in the Fluorescence Module and cannot be exchanged. In addition, there is a fluorescence module which does not include filters so that the user can insert their own preferences. The UV protection filter prevents reflected light harming the user’s eyes. Fluorescence Module for: Part No. Excitation Filter Dichroic Mirror Barrier Filter GFP fluorescence 10 446 093 425/60 nm 470 nm 475 nm GFP Plus fluorescence 10 446 143 480/40 nm 505 nm 510 nm GFP Plant fluorescence 10 446 234 470/40 nm 495 nm 525/50 nm UV fluorescence 10 446 144 360/40 nm 400 nm 420 nm Violet fluorescence 10 446 145 425/40 nm 460 nm 475 nm Blue fluorescence 10 446 146 470/40 nm 505 nm 5a15 nm Green fluorescence 10 446 147 546/10 nm 565 nm 590 nm Fluorescence module without filter set 10 446 159 UV protection filter 10 446 154 QUICK INFO Order Number..........................see list left System Diagram....................... click here Catalog Reference................... click here 65 4.2 Leica Fluorescence Module 2/3 Leica Fluorescence Module In addition to the fluorescence modules, sets can be ordered separately. Fluorescence Module for: Tube factor The Tube factor for the Fluorescence Module is 1.0 × Part No. Excitation Filter Dichroic Mirror Barrier Filter GFP fluorescence 10 446 148 425/60 nm 470 nm 475 nm GFP Plus fluorescence 10 446 149 480/40 nm 505 nm 510 nm GFP Plant fluorescence 10 446 235 470/40 nm 495 nm 525/50 nm UV fluorescence 10 446 150 360/40 nm 400 nm 420 nm Violet fluorescence 10 446 151 425/40 nm 460 nm 475 nm Blue fluorescence 10 446 152 470/40 nm 505 nm 515 nm Green fluorescence 10 446 153 546/10 nm 565 nm 590 nm CFP Filter set 10 446 363 436/20 nm 460 nm (DCLP) 480/40 nm YFP Filter set 10 446 364 510/20 nm 515 nm (LP) 560/40 nm Texas red filter set 10 446 365 560/40 nm 595 nm (DCLP) 610 LP nm Filtercarrier for Fluorescence Module without filters 10 446 186 QUICK INFO Order Number...................... see page 1/3 System Diagram....................... click here Catalog Reference................... click here 66 4.2 Leica Fluorescence Module 3/3 Leica Fluorescence Module Modularity The Fluorescence Module can be easily attached between the binocular tube and the stereomicroscope body. The interfaces are the same as for the accessory tubes such as coaxial illuminator. The Fluorescence Module can be attached to all existing stereomicroscopes including the Leica MZ APO, MZ12, MZ125, MZ95, MZ8, MZ75, MZ6, MS5 and to the old WILD Stereomicroscopes, but not to the WILD M5A. Limitations The left optical system interferes slightly with the exciting light. This causes minor reflexes in the left image. They can be minimized by using a diaphragm between the Fluorescence Module and the stereomicroscope body. For GFP observation, the specimen must be transparent UV protection filter The UV protection filter should be attached to the clampable arm (10 399 211). The arm can be attached to the lampholder (10 445 654) which is fitted to the column. Please position the shield so that the operator cannot look directly into the illuminated spot. Refer to the position of the UV protection filter in the picture to the right. QUICK INFO Order Number...................... see page 1/3 System Diagram....................... click here Catalog Reference................... click here Light sources The light souce required for the Fluorescence Module is a Hg-high-pressure bulb with the lamp house 105Z from Leica Wetzlar. Here you can choose between a 50 W and a 100 W version. Please note that the 100 W version is available with two different sockets for the bulb. If you use the 100 W version with the large socket, place the microscope carrier in the upper position. Please compare this with Recommendations, Point 2. 67 4.3 Leica MZ16 F / FA Leica MZ16 F / FA 100% apochromatic Worldwide, optical systems from Leica Microsystems are synonymous with first-class image performance, brilliant image quality and high viewing comfort. In the Leica MZ16 F and MZ16 FA, the complete imaging system, including 16:1 zoom, objective and ErgoTube®, is apochromatically corrected using high technology. Only a fully apochromatic optics system, images fine structures clearly and in detail without interfering colored edges. Contrast, richness in detail, resolution, image and color fidelity are optimum and expand the three-dimensional view of the inside of a cell. The planapochromatic top objectives support the high optical performance and light utilization. Greatest zoom, highest resolution available Leica MZ16 F and MZ16 FA are the fluorescence stereomicroscopes with the largest zoom ranges: In many cases, after sorting and processing the specimens, you no longer have to change to a light microscope to be able to precisely characterize details and reactions. This makes the work procedure significantly easier, saving valuable time. Evaluate and manipulate the living specimens in their entirety first and then three dimensionally, both with a large depth of focus and a wide field of view. Next, change to 115× magnification (with planapo 1× objective and 10× eyepieces) and analyze the finest fluorescence signals at a resolution of 420 pairs of lines/ mm. The planapo objective 2× provides a 230× magnification, a resolution of 840 pairs of lines/mm, a visible structure width of 0.6 micron and a numeric aperture of 0.28. Patented third beam path ensures intensive illumination TripleBeam® is the patented third beam path in Leica MZ16 F and Leica MZ16 FA. It is exclusively reserved for fluorescence illumination and guarantees maximum light utilization at each zoom setting with uniform and reflection free fields of view. This unique innovation form Leica Microsystems provides intensely detail rich fluorescence images on jet-black backgrounds. Image information in new dimensions As the worldwide leading manufacturer of innovative precision instruments, Leica Microsystems has a clear goal in its sight: Making image information accessible with an imaging quality that goes beyond the ordinary. Since the typical model organisms are generally low in contrast, the structural portions are made visible only by using optics with special qualities. For this reason, the Leica MZ16 F and MZ16 FA are always equipped with planapochromatic objectives 1×, 2×, 1.6× or 0.63× and multiple-coated wide-field eyepieces 10×/21, 16×/14, 25×/9.5, 40×/6 for users wearing glasses. Soft clip-on eyecups protect glasses from scratches and prevent eye infections in cases where several users work with the same instrument. QUICK INFO Order Number..................... see brochure System Diagram....................... click here Catalog Reference................... click here Detailed Information and System Diagram with Order Numbers see Brochure M1-116-5 68 5 Leica LED Solutions 5.1 Introduction 5.2 LED1000 Solutions 5.3 Leica VisiLED 5.4 Leica LED2000 5.4 Leica  LED2500 5.1 Introduction Advantages of the LED Illumination Currently and within several market segments, there is a strong trend towards using power LEDs. High brightness LEDs offer many potential advantages over traditional incandescent bulbs and fluorescent tubes. Not only are they highly reliable, with a long service life, but they consume relatively little energy. A lower operating voltage also means they are safer to handle, projecting less heat within the illuminated field. Whilst LEDs are still relatively expensive, particularly in the case of Power LEDs, they are becoming more affordable. LED technology is still quite new and does need for further engineering, however there will be a surge of rapid improvements which will be beneficial to the stereomicroscopy business. Below are the main advantages of the LEDs: • Long service life (25,000 hours plus) • Daylight quality (5000 to 6500 K) • No thermal radiation of the specimen (no radiation of UV or IR) • Compact design • Vibration proof as no fan integrated • Constant color temperature (throughout its lifetime and at dim settings) • Resistant against ripples from the electricity supply (flicker free operation) • Operation without noise 70 5.2 LED1000 Solutions LED1000 The LED1000 creates new standards for stereomicroscopes by providing daylight quality illumination along with additional unique features: • Daylight light quality (5.000 K) • Flicker-free illumination • Ripple-free illumination • “Cold light” • Continuous brightness control • Long lifetime of the bulbs, as typical for LED’s • Operation without noise and vibration • Extremely compact design • No breakable or sensitive light guides • Antistatic ringlight and spotlight-housing • Possibility of mobile operation, by using a 24 V battery • Power supply with automatic calibration for operation from 100 - 240 V • Modular system • Extensive range of accessories • Excellent price-performance ratio With the LED1000, Leica offers the following: • LED1000 Spotlight • LED1000 Ringlight • LED1000 Transmitted light stage (click each point to reach the referring page) QUICK INFO Order Number................. see links in text System Diagram....................... click here Catalog Reference................... click here 71 5.3 Leica VisiLED Leica VisiLED The VisiLED MC series is an innovative illumination system using white LEDs. Specially developed for the fields of stereomicroscopy and macroscopy, this system opens up completely new possibilities for putting your specimen in the right light. The excellent electronic control of the LED gives the MC Series contrast methods which go far beyond the possibilities provided by conventional microscope illumination. Cold, high quality white light, is brought precisely to the specimen exactly as you would expect from Schott. Structured illumination for brightfield and darkfield The modular system offers four ringlights for different applications: two ringlights for intensive incident illumination (working distance 25–50 mm or 55–135 mm) and a ringlight for lower brightness requirements. An additional ringlight for real incident darkfield (working distance 10 mm) ensures optimum illumination of flat-structure specimens. The LED transmitted light stage provides homogenous and suitable light for your application in transmitted light. • • • • • • • • controllable in segments easily adaptable to microscope objectives homogenous illumination of your object field best white light quality: typically 6,000 K excellent light values: up to 100 lm total light flux. Illumination intensity of ringlights up to 120 klx light density of transmitted light stage up to 8,000 cd/m2 long operating life: typically 10,000 h by actively controlling the LED chip temperature QUICK INFO Order Number.....................see chapter 8 System Diagram....................... click here Catalog Reference................... click here Different Switch States of the VisiLED Ringlight Key features 72 5.3 Leica VisiLED 2/3 Leica visiled MC1500 MC750 Features The basic MC750 controller is available for low contrast requirements (including dimming and LED temperature control). • • • • Features • • Continous dimming via potentiometer Overheating protection of the LED lighthead • • • • • • MC1500 The MC1500 controller offers innovative possibilities for contrasting your specimen. By steering up to two illumination heads, the MC1500 switches between brightfield and darkfield or targeted mixing of incident and transmitted light illumination. 5 preset modes plus rotation for optimum contrasts Smooth dimming with overdrive positioning 4 memory positions to save customised lighting modes Temperature control and overheating protection for LED lightheads External Trigger: to start single strobe Flash mode: to overdrive continuous set intensity with a short flash for taking photographs Strobe mode: to utilize internal strobe function with frequencies between 15 Hz–5 KHz RS 232 Computer interface (USB 1.1 optional) to set parameters via computer Foot switch to switch between memory positions (optional) Manufactured in the EU. The MC1500 controller can be steered completely by PC or laptop using the provided Windows demo software. Alternatively the enclosed DLL-library can be integrated into any customer specific software. After downloading to the memory keys of the MC1500 the chosen settings are available on the controller – even without further connection to a PC. The software enables users to operate all controller properties and several extra functions by remote control such as individual segment control, strobe duration and intensity, rotation frequency etc. QUICK INFO Order Number.....................see chapter 8 System Diagram....................... click here Catalog Reference................... click here 73 5.3 Leica VisiLED 3/3 Leica visiled MC1500 Accessories for VisiLED Footswitch • • Enables the user to remotely switch between stored memory positions (1 through to 4) quickly and change the stored illumination conditions. Allows the user to quickly and easily change various illumination types by allowing total hand control under the microscope. Flash Cable • • Allows the MC1500 to be connected to a photo camera, CCD camera or other photographic equipment via a Scontact Assists externally triggered flash and trigger mode QUICK INFO Order Number.....................see chapter 8 System Diagram....................... click here Catalog Reference................... click here RS-232 USB 1.1 Converter Cable • • • Connects the serial plug of the MC1500 controller to the USB plug of a computer. Full USB 1.1 functionality will be maintained. Driver is included. 74 5.4 Leica LED2000 Leica LED2000 The Leica LED2000 is a robust stand including an incident LED light especially made for the StereoZoom line. The integrated power supply makes this stand a complete “all in one solution”. Different contrast for different results The innovative LED illumination of the Leica LED2000/2500 bases provides a variety of illumination options. The incident light diodes can be activated as shown in the 4 examples below. The first setting (all 7 LEDs) provides high brightness for dark samples. The setting shown in the 2nd picture delivers similar homogeneity to that of a standard ring light. The next setting activates the sophisticated arc illumination, which delivers different contrast results. The varying contrast significantly increases the 3D impression of the sample giving a detailed view of the sample. The built-in incident light can be used individually or in combination with the transmitted light with the LED2500. It is also possible to dim the light to desired brightness. With the light manager touch panel, versatile illumination settings can be reproduced exactly. Key–Features • • • • • • All in one solution (stand, illumination, power supply) No fibre light guides or external cold light sources neededCompact design for easy movement to different working places Innovative power LED illumination Low profile base plate with large working area Integrated touch panel to control the different light settings Integrated universal power supply (100 – 240 V) 120 mm interface for accessories Innovative power LED illumination • • • • • • Built in 4-point top light for homogeneous and bright light conditions (like a ring light) Adjustable 3-point arc illumination to achieve different contrasts (like a gooseneck) Daylight quality color temperature at 6500 K Noise and vibration free solution (no fan integrated) Maintenance free (minimum of. 25,000 hours service life time) Reproducible light conditions QUICK INFO Order Number........................... 10 447 347 Diagram...................................... click here Catalog Reference................... click here 75 5.4 Leica  LED2500 Leica LED2500 The LED2500 offers the same high performance of the LED2000 stand. In addition, it includes both incident and transmitted light capabilities which can either be used together or controlled separately. Additional key features of the LED2500 • • • • • Integrated LED transmitted light 60 mm active light diameter Same base plate height as the incident light version Resistant against water splashes Color temperature of 5500 K Both LED stands need a power cord. Please order the country pending power cord separately. Power Cords 10 445 662 10 445 661 10 280 636 10 445 663 10 447 346 10 450 012 10 450 013 10 450 014 10 450 015 10 450 016 10 450 017 2 m, EURO 2 m, USA 2.5 m, Switzerland 2 m – 2.5 m, BS 2 m, Japan Power cable Argentina Power cable Australia Power cable China Power cable Israel Power cable Italy Power cable South Africa QUICK INFO Order Number........................... 10 447 348 Diagram...................................... click here Catalog Reference................... click here Image of a Cell Section taken with LED2500 Transmitted Light 76 6 Cold Light Solutions 6.1 Leica L2 6.2 Photonic Cold Light Sources 6.3 Schott Cold Light 6.3.1 Schott KL1500 83 6.3.2 KL2500 85 6.1 Leica L2 The Leica L2 can be attached directly to the stereomicroscope as an antistatic cold light source. If the stereomicroscope itself is one of the Leica antistatic models, the combination is ideal for electrostatically-delicate tasks. The Leica L2 offers better performance at a lower price than other cold light sources in the 20 W class. Adjustment of light intensity Double gooseneck with flexible lightguides enable a very individual position of light on the object • Highest total light stream of 63 lumens at light guide – more intense light to produce better microscope images • Minimal 100 Hz flickers, no light dispersion, constant color • temperature at 3200° K for optimized video images • Smallest (125 mm × 110 mm × 150 mm) and lightest (0.5 kg) • cold light source – conserves bench space, easy to move • Can be integrated with stereomicroscopes to provide optimized illumination, taking up less space and avoiding illumination being misdirected during focusing automatic calibration to the corresponding network voltage • Antistatic casing in harmony with antistatic Leica stereomicroscopes • for ESD safe inspections • Long lamp life of 250 hours (at maximum intensity) to 5000 hours (at level I) saves money, simple lamp changing (no tools required) saves time • Noise-free and vibration-free operation for crystal clear images in a peaceful environment • Various light guides with a protective coating of self-erasing Megolon® – long life fibre guides to accurately direct the light where it is most needed • Attachments for coaxial, vertical, and transparent illumination. Special lighting techniques to see details that may otherwise go unnoticed Leica l2 coldlight QUICK INFO Bestellnummer......................... 10 446 385 System Diagram....................... click here Catalog Reference................... click here 78 6.2 Photonic Cold Light Sources The new CLS X series from Leica represents a universally applicable illumination system that is matched to the complex demands of science and technology. The innovations of the CLS X series from Leica guarantee a new dimension with respect to light utilization, light quality and system costs. Top performances in cold light illumination can only be achieved using a perfectly designed total system. This includes light source, light guide and varied accessories. During further development of the system, special attention has been paid to high-performance light sources and, in particular, to powerful light guides. Innovative features of the light guides allow the cost-effective use of the light source CLS50 X, in power ranges that were previously reserved for light sources with a power consumption of 150 W. With a useful lamp life that lies far above that of comparable products, the new CLX series is extremely good value. The cold-light sources and light guides are compatible with all Leica stereomicroscopes. 79 6.2 Photonic Cold Light Sources CLS150 X CLS150 X • • • • • • • The ideal cost-effective variant that can compete with units of a higher power level if used in conjunction with the newly developed light guides Electronic brightness control Operates without fan and is particularly suitable for applications demanding a vibration-free and low-noise environment CLS 100 X • • • • • Powerful and economical Uniform illuminated area with constant color temperature Continuous brightness control by using a mechanical diaphragm Exceptionally long bulb service life Fan-cooled light source CLS100 X Photonic Cold Light Sources Perfect luminous efficiency at maximum economic efficiency Mechanical and electronic brightness control Constant and adjustable color temperature Fan-cooled light source CLS 150 XD • • • • • • • • • • CLS50 X 2/3 Additional power increase Bulb features a larger voltage range (100 – 240 V) Power pack is ripple-free Features electronic and mechanical brightness control Color temperature is shown on a display Connection for hand-held remote control with memory functionbrightness control Remote socket for serial interface (RS 232) for computerbased control Synchronous control of several units possible Suitable for use in cleanrooms Additionally equipped with swing-in lens CLS150 X QUICK INFO Order Number........................... see p. 100 Diagram...................................... click here Catalog Reference................... click here CLS150 XD 80 6.2 Photonic Cold Light Sources 3/3 Photonic Cold Light Sources CLS150 XE • • • Continuous electronic brightness control Adjustable color temperature Potentiometer allows for voltage regulation, thereby increasing the bulb service life CLS 150 LS The CLS150 LS has been specially adapted with automated control of the new transmitted light base TL RCI™ via LAS. Via the serial port, the brightness, color temperature and electronic shutter can be controlled directly from the PC or Leica UMC. QUICK INFO Order Number........................... see p. 100 Diagram...................................... click here Catalog Reference................... click here CLS150 XE CLS150 LS 81 6.3 Schott Cold Light 82 6.3.1 Schott KL1500 Schott KL1500 The KL1500 LCD is the performance leader in the field of 150watt halogen coldlight sources. With its outstanding modern design combined with optimized, homogeneous light output and variable light control options, this light source has been designed for applications in Stereo Microscopy and Macroscopy in laboratory, industrial and medical applications. The KL1500 LCD is available in 120 and 230 V versions. The KL1500 LCD has been approved and certified as a laboratory and electromedical device by VDE (230 volt version) and CSA (C/US) (120 volt version). The 230 volt version carries the CE conformity label. • • • • • • Easy and reproducable operation. Functional lamp compartment for quick lamp replacement. Filter drawer for selection of color filters. Swing-in input optics for homogenization of light input. Easy stacking capability with access to all features and functions. The design of a low-noise fan in combination with optimum air venting at the back of the unit helps contribute to quite and pleasant working conditions. Approvals The KL2500 LCD has been approved and certified as a laboratory and electromedical device by VDE (230 volt version) and CSA (C/US) (120 volt version). The 230 volt version carries the CE conformity label. QUICK INFO Order Number.....................see chapter 8 Diagram...................................... click here Catalog Reference................... click here 83 6.3.1 KL1500 2/2 Schott KL1500 QUICK INFO Order Number......................see page 106 Diagram...................................... click here Catalog Reference................... click here 84 6.3.2 KL2500 Schott KL2500 With its new design and unique performance spectrum, the KL2500 LCD is setting a new benchmark in the field of halogen cold light sources. Fittted with a 250-watt cold light reflector lamp, the KL2500 LCD transmitts more than twice the amount of the light of the KL1500 LCD. This unparalleled performance makes the KL2500 LCD the instrument of choice for the most chanllenging ap-plications in Stereo Microscopy, Macroscopy and Forensics. The KL2500 LCD is equipped with a 5-position filter wheel, which makes filter selcection quick and easy. Color filters and special GFP-fluorescence excitation filters are also available. Electronic accessories such as remote control or tilt switch can be connected to the analog socket in the front panel, enabling remote light source operation or connection of an RS232-Interface. The KL2500 LCD has been approved and certified as laboratory and electromedical device by VDE (230 volt version) and CSA (C/US) (120 volt version). The 230 volt version carries the CE conformity label. • • • • • Ease of use: The KL2500 LCD is supplied with a filter wheel, which accomodates up to five insert filters. Filter selection is as simple as a turn of the knob. Electronic accessories, such as remote control, RS-232 or tilt switch, can be connected to the front panel of the device enabling light source operation in difficult access areas. With the modified light guide socket, light guides up to 15mm fiber bundle diameter can be used with optimum results. Design compatibility allows KL1500 LCD light guides to also be used with the KL2500 LCD. All other functions are identical to the KL1500 LCD. Approvals QUICK INFO The KL 2500 LCD has been approved and certified by VDE and UL (120-Volt version for U.S. and Canada) and carries the CE conformity label (230-Volt version). Manufactured in the EU. Order Number......................see page 106 Diagram...................................... click here Catalog Reference................... click here 85 6.3.2 KL2500 2/2 Schott KL2500 QUICK INFO Order Number......................see page 106 Diagram...................................... click here Catalog Reference................... click here 86 7 Accessories 7.1 Adapter to mount fibre light guides 7.2 Ringlight Adapter for Objectives 7.3 Adapters for swing arm stands 7.4 Color Filter for CLS 7.5 Base Plate for CLS 7.6 Segmentation attachment for continuous slit ring lights (CLS) 7.1 Adapter to mount fibre light guides adapter for fibre light guides Solutions from Schott Solutions from Photonic Light guide holders and focus lens holders Adapter and mounts for light guides 30 157 105 Adapter M8 / M6 31 158 330 Holder for light guide up to 5 mm with thread M6 for articulating arm 31 158 335 Holder for light guide up to 8 mm with thread M6 for articulating arm 31 157 430 Holder f. flex. light guide 12 mm, 15 mm with thread M6 for articulating arm 31 158 341 Holder for focusing lens with thread M6 for articulating arm 31 158 345 Articulating arm w. attachment screws M6 31158340 Base for articulating arm, 3 threads M6 (cast foot with 3 threads M6) 31158220 Holder for focusing lens (for focussing lens 10 446 391 and articulating arm 10 399 211) 10399211 Arm, clampable, for lampholder (suitable articulating arm for column clamp 10 445 654 and focus lens holder 31 158 220) 30 143 001 30 143 002 30 157 101 30 157 102 30 157 103 30 157 104 30 157 105 30 157 202 Adapter-screw for lamp-holder (10427589) Light guide adapter for column d = 25 mm Articulated support arm L=200 mm Base plate for support arm Holder for illumination light guide Adapter for holder Adapter M8 / M6 Adapter with focussing lens for sub base 30 117 701 Adapter for Schott Light guides QUICK INFO Order Number.........................see list left System Diagram................ see chapter 8 Catalog Reference............ see chapter 8 Accessories for ring lights 31 157 430 Holder f. flex. light guide 12 mm, 15 mm (suitable articulating arm 31 158 345) (in combination with the column clamp 10 445 654 and articulating arm an adapter M8/M6 is required!) 88 7.2 Ringlight Adapter for Objectives Ringlight adapters for objectives List of Adapters 30 123 107 Adapter for ringlight 66 / 58 mm QUICK INFO Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here 89 7.3 Adapters for Swing Arm Stands adapter for Comb. light guides It is quite critical to bring the light to the correct position with swing arm stands. For this we recommend the use of combination light guides. Photonic (goose neck with 2 arms, light guide length 750 / 1000 / 1500 / 2000 mm) 30 140 001 30 140 002 30 140 003 30 140 004 Combination light guide 2arms, 4.5 mm /750 mm Combination light guide 2arms, 4.5 mm /1000 mm Combination light guide 2arms, 4.5 mm /1500 mm Combination light guide 2arms, 4.5 mm /2000 mm Schott 31 154 402 Combination light guides, 2 arm 600 mm goose neck with 2 arms 600 mm for illumination, 1 arm 850 mm for connection with the cold light source (attaching onto the column with clamp 10 445 654!) QUICK INFO Order Number.........................see list left System Diagram................ see chapter 8 Catalog Reference............ see chapter 8 90 7.3 Adapters for Swing Arm Stands 2/2 adapter for swing arm stands 30 143 005 Adapter for 35 mm column and routine horizontal arm 30 143 004 Adapter for standard and large ball bearing horizontal arm This adapter fits to: 10447008 vertical column and 10447097 routine horizontal arm This adapter fits to 10447098 standard ball bearing horizontal arm 10447099 large ball bearing horizontal arm QUICK INFO Order Number.........................see list left System Diagram................ see chapter 8 Catalog Reference............ see chapter 8 Adapter to mount fibre light guides on Large Swingarm Stand (adapter mounted in upper position). Adapter to mount fibre light guides on ESD Swingarm Stand. 91 7.4 Color Filter for CLS Color Filters for cls The color filter, for the Leica CLS cold light sources have changed. The glasses for the old filters are no longer available and therefore we have launched a new generation of color filters. Glasses have a coating on their surfaces to create color (In former times the glass filters were fully stained). Due to this, filter characteristics are different meaning that the colors are not 100 % the same as before! The new articles numbers for the focusing lenses 30 161 113 30 161 114 30 161 115 30 161 116 Filter for focussing lens, blue Filter for focussing lens, green Filter for focussing lens, yellow Filter for focussing lens, red Filter for CLS lamps (insertion into the filter slot) 30 117 211 30 117 212 30 117 213 30 117 214 Filter blue Filter green Filter yellow Filter red QUICK INFO Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here 92 7.4 Color Filter for CLS 2 /2 color filters for cls QUICK INFO Order Number.........................see list left System Diagram....................... click here Catalog Reference................... click here The transmission curves shown above, gives the characteristic for each of the color filters. This is important in stereomicroscopy in combination with cold light sources, as the visible light spectrum only extends from 450 nm to 750 nm. This is only a very small part of the whole electromagnetic and visible spectrum. 93 7.5 Base Plate for CLS Base Plate for CLS Sometimes when using goosenecks, the cold light source is not heavy enough to comfortably move the gooseneck arms. To increase the weight of the CLS cold light source, we have an additional base plate available. This base plate is fixed under the CLS lamp. First the original screws (A) need to be removed with a screwdriver. The base plate is then fixed beneath the cold light source with the screws provided. This base plate fits to all CLS cold light sources. QUICK INFO Order Number...........................30 117 209 System Diagram....................... click here Catalog Reference................... click here 94 v 7.6 Segmentation Attachment for Continuous Slit Ring Lights (CLS) Segmentation Attachment The advantage of this attachment is that the customer can reproduce light conditions and optimize the light and shadow effect in the specimen level. The segmentation attachment can be used for the following ring lights: 30 120 201 / 30 120 202 / 30 120 203 / 30 120 204 It is possible to cover a part of the slit ring light (between 120° and 240°, click stops on 150°, 180°, 210°) and rotate this light cover 360° until the correct position is found to illuminate the specimen. This item is primarily used in forensics and quality control. The focusing attachment must be removed before the segment attachment can be fixed. QUICK INFO Order Number.......................... 30 121 202 System Diagram....................... click here Catalog Reference................... click here variable cover size 360° revolvable focussing attachment must be removed 95 8 System Diagrams and Catalog References 8.1 Leica LED1000 8.2 Leica VisiLED 8.3 Fluorescent Ringlight 8.4 Leica L2 8.5 Photonic Cold Light Sources 8.6 Schott Cold Light Sources 8.7 Leica Transmitted Light Bases 96 8.1 Leica LED1000 30 221 005 30 221 007 97 8.1 Leica LED1000 30 210 001 30 210 002 30 220 001 30 240 001 Power supply with control unit LED ringlight (incl. adapter 66 / 58 mm) LED spotlight LED backlight 30 117 102 30 117 103 30 117 104 30 117 105 30 117 106 30 117 107 30 221 005 30 221 006 30 221 007 30 221 008 Power cord USA/Can/J Power cord Italy Power cord EU Power cord Switzerland Power cord UK Power cord Australia Goose neck L = 85 mm Goose neck L = 200 mm Goose neck L = 300 mm Base plate for M5 screw Catalog Reference 30 221 001 30 221 002 30 221 003 30 221 004 30 122 102 30 231 002 Filter blue Filter green Filter yellow Filter red Clamping ring for LED ringlight Screw for clamping ring to ringlight 30 211 001 Control unit (spare part) 30 211 002 Power supply (spare part) 30 123 101 30 123 102 30 123 103 30 123 104 30 123 105 30 123 106 30 123 107 30 123 121 30 123 131 30 231 001 Adapter for MS5 / MZ6 (Achr. obj. 1.0×/0.8×/0.63×/0.32×) Adapter for MS5/MZ6 (Achr. obj. 1.5×) Adapter for MS5/MZ6 (Achr. obj. 2.0×) Adapter for M420 (Obj. Makro/Apo Zoom) Adapter for M5A (Add. obj. 2.0× /1.5× /0.5×) Adapter for MZ6 Obj. 411597 Adapter for ringlight 66/58 mm Adapter for GZ6 (only for working distance 150 mm and more) Adapter for GZ4 Polarizing filter set 98 8.2 Leica VisiLED 99 8.2 Leica VisiLED 31 400 010 Basic controller MC750 with smooth dimming and integrated LED tempera- ture control, for all LED illuminators 31 400 000 Multifunction controller MC1500 with five segment modes, smooth dimming, four memory positions, integrated LED temperature control, flash and strobe function, PC interface (RS 232; USB optional) and connectors for footswitch and two illumination systems, inclusive CD-ROM with demonstration software for PC control, for all LED illuminators 31 400 050 Power supply 100 - 240 VAC, 50/60 Hz for Multifunction controller or basic controller output 24 V/1 A, UL and CE safety-tested, for both controllers (power cable required!) Adapter for objectives 31 400 510 31 157 450 31 157 422 Adapter 70/66 mm for LED darkfield ring light Objective adapter for ring light 58/66 mm Objective adapter for ring light, M420 Mains cable 10 280 636 10 445 661 10 445 662 10 445 663 Mains cable, 2.5 m, Switzerland Mains cable, 2 m, USA Mains cable, 2 m, EURO Mains cable, 2 m-2.5 m, BS LED ring light 31 400 100 31 400 225 31 400 255 31 400 300 LED ring light for brightfield S40-55 for objectives with 66 mm external dia- meter, working distance 55 - 110 mm LED ring light for intensive brightfield S80-25 for objectives with 66 mm external diameter, working distance 25 - 50 mm LED ring light for intensive brightfield S80-55 for objectives with 66 mm external diameter, working distance 55 - 135 mm LED ring light for intensive darkfield S40-10D for objectives with 70 mm external diameter, working distance 5 - 15 mm (for objectives with 66 mm adapter 31400510 is required!) Combikit for incident brightfield and incident darkfield illumination 31 400 500 Combikit for VisiLED illumination Accessories for LED illumination 31 400 020 31 400 030 31 400 035 Foot switch for LED illumination for switching the memory positions RS 232 USB 1.1 converter for LED illumination Synchronous cable for flash, s-contact for photo flash synchronization 100 8.3 Fluorescent Ringlight 30 310 001 30 310 009 30 310 010 30 211 002 30 310 002 30 310 007 30 310 008 30 310 003 30 310 004 30 310 005 30 310 006 30 320 001 30 320 002 30 221 008 30 117 102 30 117 103 30 117 104 30 117 105 30 117 106 30 117 107 Fluorescent ring light with control unit (standard) Fluorescent ring light with control unit HP Fluorescent ring light with control unit UV Power supply 100-240 V AC, 24 V DC/15 W Replacement bulb (standard) (spare-part) Replacement bulb UV (spare-part) Replacement bulb HP (spare-part) ESD protective grid Mounting ring 40–66 mm ESD Spare screw M4 × 16 f. mounting ring 66 Spare screw M4 × 25 f. mounting ring 40–66 Mounting adapter for goose neck mount Goose neck M5 200 mm Base plate with M5 adapter for goose neck Mains cable 3-pin IEC320 for USA/CAN/J Mains cable I Mains cable EU Mains cable CH Mains cable UK Mains cable AUS 101 8.4 Leica L2 10 446 385 10 447 015 10 446 386 10 446 387 10 446 388 10 446 389 10 446 390 10 447 038 10 446 391 10 447 055 10 446 392 10 447 152 10 446 374 10 446 375 10 446 376 10 446 377 10 446 378 10 447 009 10 446 372 10 446 373 10 280 636 10 445 661 10 445 662 10 445 663 10 445 012 10 445 013 10 445 014 10 445 015 10 445 016 10 445 017 Leica L2 fiber optic light source Power supply Leica L2 Single flexible light guide, 550 mm Double flexible light guide, 750 mm Single gooseneck, 500 mm Double gooseneck, 500 mm 6-point ringlight, 58mm I.D., 750mm Transmitted light stage Focusing lens Daylight conversion filter for focusing lens Universal light guide Double universal light guide L2 adapter for focus column L2 adapter for S-stand L2 adapter for focus drive 300 mm L2 base Flexible light guide mount Universal light guide mount Near vertical illuminator Coaxial illuminator Power cable 2 m, CH Power cable 2 m, US Power cable 2 m, EURO Power cable 2 m, BS Power cable 2 m, Argentina Type K Power cable 2 m, Australia Type F Power cable 2 m, China Type L Power cable 2 m, Israel Type I Power cable 2 m, Italy Type E Power cable 2 m, South Africa Type D Bulb 10 447 056 Bulb 8V/20W for L2 102 8.5 Photonic Cold Light Sources 103 8.5 Photonic Cold Light Source Cold light sources 30 111 150 30 111 160 30 111 250 30 111 251 30 111 260 30 111 350 30 111 360 30 111 450 30 111 460 30 111 480 CLS 50X MED (230 V-240 V) CLS 50X MED (120 V) CLS100X/MED(230-240 V) CLS100X/MED(230-240 V) UK version CLS100X/MED(100 V-120 V) CLS150X/MED(230 V-240 V) CLS150X/MED(100 V-120 V) CLS150XE MED( 230 V-240 V) CLS150XE MED (100 V-120 V) CLS150XD MED (100-240 V) Base plate for CLS 30 117 209 Base plate for CLS cold light sources Mains cables 10 280 636 10 445 661 10 445 662 10 445 663 30 117 107 30 117 103 Mains cable, 2.5 m, Switzerland Mains cable, 2 m, USA Mains cable, 2 m, EURO Mains cable, 2 m-2.5 m, BS Power cable for Australia Power cable for Italy Filter with holder for cold light sources 30 117 211 30 117 212 30 117 213 30 117 214 30 117 205 30 118 701 Filter holder with filter blue Filter holder with filter green Filter holder with filter yellow Filter holder with filter red Filter holder with filter daylight Filter holder empty (for CLS, MZ FLIII and MZ16 FA) Remote access for CLS150 XD 30 117 601 30 117 602 30 117 603 Remote control for CLS150D,XD Electronic connector cable for CLS150D,XD Interface RS232 for CLS150D,XD Schott interface for using Schott fibre light guides 30 117 701 Adapter for Schott Light guides Flexible light guides 30 150 111 30 150 112 30 150 113 30 150 114 30 150 121 30 150 122 30 150 123 30 150 124 30 150 131 30 150 132 30 150 211 30 150 212 30 150 213 30 150 214 30 150 221 30 150 222 30 150 223 30 150 231 Flex light guide 1arm 3 mm/600 mm Flex light guide 1arm 3 mm/1000 mm Flex light guide 1arm 3 mm/1500 mm Flex light guide 1arm 3 mm/2000 mm Flex light guide 1arm 5 mm/600 mm Flex light guide 1arm 5 mm/1000 mm Flex light guide 1arm 5 mm/1500 mm Flex light guide 1arm 5 mm/2000 mm Flex light guide 1arm 8 mm/1000 mm Flex light guide 1arm 8 mm/1500 mm Flex light guide 2arms 3 mm/600 mm Flex light guide 2arms 3 mm/1000 mm Flex light guide 2arms 3 mm/1500 mm Flex light guide 2arms 3 mm/2000 mm Flex light guide 2arms 5 mm/600 mm Flex light guide 2arms 5 mm/1000 mm Flex light guide 2arms 5 mm/1500 mm Flex light guide 2arms 8 mm/1000 mm 30 150 232 30 150 233 30 150 701 30 150 702 30 150 703 30 150 704 30 150 711 30 150 712 30 150 713 Flex light guide 2arms 8 mm/1500 mm Flex light guide 2arms 8 mm/2000 mm Flex light guide 1arm TVC 9 mm/500 mm Flex light guide 1arm TVC 9 mm/750 mm Flex light guide 1arm TVC 9 mm/1000 mm Flex light guide 1arm TVC 9 mm/1500 mm Flex light guide 1arm TVC 5 mm/500 mm Flex light guide 1arm TVC 5 mm/750 mm Flex light guide 1arm TVC 5 mm/1000 mm Goose necks 30 130 011 30 130 012 30 130 021 30 130 022 30 130 031 30 130 422 Goose neck 1arm 4.5 mm/600 mm Goose neck 1arm 3 mm/500 mm Goose neck 2arms 3 mm/500 mm Goose neck 2arms 4.5 mm/600 mm Goose neck 3arms 4.5 mm/600 mm Goose neck 2-arms 4.5 mm/600 mm ESD Combination light guides (goose neck with 2arms, light guide length 750/1000/ 1500/2000 mm) 30 140 001 30 140 002 30 140 003 30 140 004 Comb. light guide 2arms, 4.5 mm/750 mm Comb. light guide 2arms, 4.5 mm/1000 mm Comb. light guide 2arms, 4.5 mm/1500 mm Comb.light guide 2arms, 4.5 mm/2000 mm Focussing lenses and filters 30 161 101 Filter/Lens-holder 30 161 102 Focussing lens, 20 mm diameter 30 161 113 Filter blue, 20 mm diameter 104 8.5 Photonic Cold Light Source 30 161 114 30 161 115 30 161 116 30 161 107 30 161 108 30 161 109 30 161 110 Filter green, 20 mm diameter Filter yellow, 20 mm diameter Filter red, 20 mm diameter Filter daylight, 20 mm diameter Filter polarisation, 20 mm diameter Diffusor, 20 mm diameter Focussing lens, 26 mm diameter Ring light guides 30 120 101 30 120 102 30 120 103 30 120 109 30 120 201 30 120 202 30 120 203 30 120 204 30 120 205 30 120 301 30 120 401 Ringlight 6 segment RL6-66/750 Ringlight 6 segment RL6-66/1000 Ringlight 6 segment RL6-66/7,6/1500 Ringlight 6 segment RL6-66/2000 Ringlight RL-66/750 Ringlight RL-66/1000 Ringlight RL6-66/1500 Ringlight RL-66/2000 Ringlight RL-66/750 mod. for revolving nosepiece MAXI-Ringlight 12,7/600 Ringlight RL-66/750 ESD Polarisation for ring light guides 30 121 101 Polarizing filterset for RL66 30 121 102 Polarizing filter for RL66 30 121 103 Analyser filter for RL66 Diffusor for ring light guides 30 121 201 Diffusor filter for RL66 Protection for ring light guide 30 121 401 Protection glass Objective adapter for ring light guides 30 123 101 30 123 102 30 123 103 30 123 104 30 123 105 30 123 106 30 123 107 RLA-66 M-series, objectives 0.32× - 1.0× RLA-66 M-sreies, objective 1.5× RLA-66 M-series, objective 2.0× RLA-66 M420 52 CHF RLA M5A with obj.2.0×/1.5×/0.5× RLA-66, objectives WD= 100 mm and WD= 150 mm RLA 66/58 mm for S-series Fluorescence sets and accessories 30 121 301 30 121 302 30 121 303 30 121 304 30 121 305 30 121 311 30 121 312 30 121 313 30 121 314 30 121 315 30 121 321 30 121 322 30 121 323 30 121 324 30 121 325 Fluorescence filterset (420/500 nm) Fluorescence filterset (470/540 nm) Fluorescence filterset (505/580 nm) Fluorescence filterset (530/600 nm.) Fluorescence filterset (570/640 nm) Excitation filter (420 nm) Excitation filter (470 nm) Excitation filter (505 nm) Excitation filter (530 nm) Excitation filter (570 nm) Cut-off filter (500 nm) Cut-off filter (540 nm) Cut-off filter (580 nm) Cut-off filter (600 nm) Cut-off filter (640 nm) Catalog Reference 30 157 101 30 157 102 30 157 103 30 157 104 30 157 105 30 157 202 30 117 701 Articulated support arm L=200 Base plate for support arm Holder for illumination light guide Adapter for holder Adapter M8/M6 Adapter with focussing lens for sub base Adapter for Schott Light guides Spare parts for cold light sources 30 118 101 30 118 102 30 118 103 30 118 104 30 118 105 30 118 106 30 118 201 30 118 202 30 118 301 30 118 302 30 118 303 30 118 304 30 118 305 30 118 308 Halogen Lamp for CLS 50/50E/50X Halogen Lamp 90 W for CLS 100 Halogen Lamp 150 W for CLS 150/ 150E/150X Halogen Lamp for CLS 150D/150X-D-E Halogen Lamp 150 W/21 V for CLS 150M Halogen lamp 100 W for CLS 100X Lamp-socket for CLS50 Lamp-socket for CLS 100/150D Fuse 4.0A CLS50/150D 100-120 V Fuse 0.315 A, CLS50/50E/X 230-240 V Fuse 0.63 A, CLS50E/50X 100/120 V Fuse 1,0 A, CLS150/E 230/240 V Fuse 1.6 A, CLS100 100/120 V Fuse 2.5 A, CLS150/E 100/120 V Adapter and mounts for light guides 30 143 001 Adapter-screw for lamp-holder (10 427 589) 30 143 002 Light guide adapter for column d=25 mm 105 8.6 Schott Cold Light Sources 106 8.6 Schott Cold Light Source Cold light sources 31 150 200 31 250 200 31 150 201 31 250 201 31 299 999 Cold light source KL 1500 LCD, 230 V Cold light source KL 2500 LCD, 230 V Cold light source KL 1500 LCD, 120 V Cold light source KL 2500 LCD, 120 V Mains cable adapter f. KL1500/ 2500 LCD, BS (this mains cable adap- ter is required for UK only!) Spare bulbs and fuses 31 153 000 31 253 000 31 153 101 31 253 101 31 153 102 31 153 103 31 253 103 Halogen refl. bulb 15 V/150 W, KL1500 LCD Halogen refl. bulb 24V/250W, KL 2500 LCD Fuse for 230 V/T2 H 250 mA, KL1500 Fuse 230 V/T 630H mA, KL 2500 LCD Fuse for 230 V/T1 10 A, KL1500 Fuse for 120 V/T4 H, KL1500 LCD Fuse for 120 V/M 6,3 A, KL 2500 LCaD Goose neck light guides 31 154 101 Goose neck light guides 1 arm 600 mm 31 154 202 Goose neck light guides 2 arms 600 mm 31 154 302 Goose neck light guides 3 arms 600 mm Combination light guides 31 154 402 Combination light guides, 2 arm 600 mm goose neck with 2 arms 600 mm for illumination, 1 arm 850 mm for connection with the cold light source (attaching onto the column with clamp 10 445 654!) Flexible light guides 31 155 101 31 155 102 31 155 103 31 155 204 31 155 205 31 155 206 Flex. light guide, 1 arm d3/1000 mm (only for KL1500 LCD) Flexible light guide, KL 1500 LCD 1600 mm Flexible light guide, 8/1000 mm Flexible light guides 2 arms 4,5/1000 mm Flex. light guides 2 arms 4,5/1600 mm Flex. light guide 3 arms 4,5/1000 mm Suitable focussing lens for goose necks and flexible light guides 10 446 391 Focusing lens f. light guides up to 5 mm 31 158 215 Focussing lens, for light guides 8 mm Flexible light guide KL 2500 LCD (only) 31 250 101 Flexible light guide 1 arm d = 12/1000 mm 31 250 102 Flexible light guide 1 arm d = 15/1000 mm Ring lights 31 157 401 4-point ring light 30 mm inner diam. 31 157 402 4-point ring light 66 mm inner diam. 31 157 410 Continuous ring light 58 mm inner diameter best working distance between 50 and 120 mm (for the Leica MZ7.5 in combination with an achromat objective two (MZ9.5 one) adapter rings 10 446 300 are required!) Catalog Reference 31 157 420 31 157 440 31 157 445 31 257 420 31 157 406 Continuous ring light 66 mm inner diameter best working distance between 50 and 120 mm Continuous ring light Maxi 150 mm diameter best working distance between 50 and 120 mm Objective adapter for ring light Maxi (in combination with achromat objective 1.0×, 58 mm diameter) Continuous ring light d = 66 mm for KL2500 LCD (only for KL2500 LCD) Continuous ring light, real incident darkfield (suitable for objectives 70 mm and 66 mm) Objective adapter for real incident darkfield (objective diameter 58 mm) 31 157 552 Obj. adapter for real incident darkf.ield 1,0× Objective adapter for real incident darkfield (objective diameter 66 mm) 31 157 562 Obj. adapter f. real incid. darkf. 1,0× (for plan and planapo objectives) For planapochomat objective 1.6× is no adapter required! 107 8.5 Schott Cold Light Source Cross-section transformer slit with adjustable angle of inclination and adjustable supplementary lens 31 160 100 31 160 110 31 160 120 31 260 130 Cross-section transf. slit 50 × 1.2 mm Cross-section transf. slit 100 × 0.6 mm Cross-section transf. slit 200 × 0.3 mm Cross-section transf. slit 300 × 0,3 mm, KL2500 LCD Special fibre light guides 31 155 302 31 155 401 Flexible light guide for vertical illumination Light guide, HL, HF/DF bases and coax-illum. 900 mm Filter and focussing lenses 31 158 302 31 158 303 31 158 304 31 158 305 31 158 306 31 258 302 31 258 303 31 258 304 31 258 305 31 258 306 Insert filter for KL 1500 LCD, blue Insert filter for KL 1500 LCD, red Insert filter for KL 1500 LCD, green Insert filter for KL 1500 LCD, yellow Insert filter for KL 1500 LCD, daylight Insert filter d = 28 mm, blue, KL2500 LCD Insert filter d = 28 mm, red, KL2500 LCD Insert filter d = 28 mm, green, KL2500 LCD Insert filter d = 28mm, yellow, KL2500 LCD Insert filter d = 28 mm, daylight Fluorescence filters 31 258 313 Insert filter d = 28 mm, blue (484 nm), KL2500 LCD 31 258 314 Insert filter d = 28 mm, green (515 nm) KL2500 LCD 31 158 200 31 158 205 10 446 391 31 158 215 31 158 206 10 447 055 31 158 430 31 158 440 Focusing lens with filter set Polarization filter for focusing lens (5 mm) Focusing lens f. light guides up to 5 mm (goose neck or flexible light guides) Focussing lens, for light guides 8 mm Polarization filter for focusing lens (8 mm) Conversion filter for focusing lens (10 446 391) Pol. filter set f. cont. ring light 66 mm Pol. filter set f. cont. ring light 58 mm Adapter for ring lights with 66 mm diameter on objectives 58 mm diameter 31 157 422 Objective adapter for ring light, M420 31 157 450 Objective adapter for ring light 58/66 mm Accessories for ring lights 31 157 430 Holder f. flex. light guide 12 mm, 15 mm (suitable articulating arm 31158345) (in combination with the column clamp 10 445 654 and articulating arm an adapter M8/M6 is required!) Catalog Reference Light guide holders and focus lens holders 30 157 105 31 158 330 31 158 335 31 157 430 31 158 341 31 158 345 31 158 340 31 158 220 10 399 211 Adapter M8/M6 Holder for light guide up to 5 mm with thread M6 for articulating arm Holder for light guide up to 8 mm with thread M6 for articulating arm Holder f. flex. light guide 12 mm, 15 mm with thread M6 for articulating arm Holder for focusing lens with thread M6 for articulating arm Articulating arm w. attachment screws M6 Base for articulating arm, 3 threads M6 (cast foot with 3 threads M6) Holder for focusing lens (for focussing lense 10 446 391 and articulating arm 10399211) Arm, clampable, f. lampholder (suitable articulating arm for column clamp 10 445 654 and focus lens holder 31158220) Flash port for cold light source (suitable for KL1500 LCD and KL2500 LCD) 31 300 100 31 300 201 Flashport f. cold light source Combi light guide for flashport (For a complete outfit a cold light source, fibre light guide and a photo flash are required! All light guides are suitable except gooseneck fibre light guides! Suitable photo flashes from Metz: Mecablitz 54 MZ3 or Mecablitz 44 MZ2 are required). 108 8.7 Leica Transmitted Light Bases 10 447 392 10 447 391 10 445 615 (300mm) 10 446 100 (500mm) 11 101 784 10 447 276 10 446 303 10 447 275 10 447 106 (300mm) 10 447 185 (500mm) 10 446 304 10 446 228 10 447 269 10 446 301 10 446 353 10 446 176 (300mm) 10 447 041 (500mm) 10 447 305 10 446 302 10 382 130 10 361 719 10 447 368 10 446 352 10 447 390 10 446 351 10 446 350 10 447 393 10 447 394 10 447 395 10 447 342 10 446 340 10 447 400 10 446 341 10 447 398 10 447 431 M1-218-4 / 11.05 10 447 443 109 8.7 Leica Transmitted Light Bases Incident-light bases 10 446 340 10 446 341 10 447 342 10 446 350 10 446 351 10 447 390 10 446 352 Incident-light base for S series Transmitted-light base for S series transmitted-light base Incident-light base for M series TL ST transmitted-light base TL BFDF transmitted-light base TL RC™ transmitted-light base for external cold light sources TL RCI™ transmitted-light base with integrated halogen illumination Stages 10 447 269 10 446 353 10 447 368 10 447 275 10 447 276 10 447 391 10 447 392 11 101 784 Standard stage for TL BFDF, TL RC™ and TL RCI™ transmitted light bases Cross-stage for TL BFDF, TL RC™, and TL RCI™ transmitted light bases and incident light base (with adapter 10 447 368) Adapter between cross-stage and 10 447 342 incident light base Thermo stage Leica MATS TL with controller Adapter for stages with ∅120 mm Stage for LifeOnStage accessory Universal carrier for Petri dish, slides (up to four pieces) etc. Column adapter for micromanipulation 10 446 301 10 446 302 10 382 130 10 361 719 10 446 303 10 446 304 10 446 228 Sliding stage, Ø 120 mm Polarization stage, Ø 120 mm Object guide for polarization stage Compensator Red I for Pol rotating stage Cup stage, Ø 120 mm Universal carrier, Ø 120 mm Glass insert with Pol, Ø 120 mm Focusing drives 10 445 615 10 446 100 10 447 106 10 447 185 10 446 176 10 447 041 Focusing drive with 300 mm profile column for incident and transmitted light bases Focusing drive with 500 mm profile column for incident and transmitted light bases Focusing drive, fast/fine, with 300 mm profile column for incident and transmitted- light bases Focusing drive, coarse/fine, with 500 mm profile column for incident and transmitted- light bases Motorized focus drive with 300 mm column and power adapter for incident and transmitted light bases Motorized focus drive with 500 mm column and power adapter for incident and transmitted light bases Catalog Reference Filter 10 447 400 10 447 394 10 447 395 10 447 393 Day light filter for TL ST base BG38 fluorescent filter for transmitted light bases TL RC™/ RCI™ UV filter for bases TL RC™/ RCI™ Filter ND (gray filter) for bases TL RC™/ TL RCI™ Illumination 10 447 443 10 443 401 10 447 398 Leica USB mouse, freely assignable five key mouse for connections to transmitted- light bases TL RCI™ or PC USB cable to connect TL RCI™ base to a PC Foot switch with CAN bus connector Ergonomic accessories 10 447 431 Leica ErgoRest (palm rest for fatigue-free operation) 110