Transcript
INSTRUCTIONS
BX45A
ROUTINE MOTORIZED MICROSCOPE
This instruction manual is for the Olympus Routine Motorized Microscope Model BX45A. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference. AX9936
BX45A
CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read chapter 8, “ASSEMBLY” (pages 23 to 25) carefully.
IMPORTANT
1-3
— Be sure to read this section for safe use of the equipment. —
1
NOMENCLATURE
2
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
3
USING THE CONTROLS
4
5-6
7-15
3-1 Microscope Base ................................................................................................................................................................................... 7 1 Voltage Indication
2 Using the Light Intensity Preset Switch
3 Using the Filter
3-2 Focusing Block .......................................................................................................................................................................................... 8 1 Replacing the Fine Adjustment Knob
2 Adjusting the Coarse Adjustment Knob Tension
3 Pre-focusing Lever
3-3 Motorized Revolving Nosepiece ............................................................................................................................ 9-10 1 Adjusting the Confocality of Objective
2 Centering the Objective
3 Switching the Objective
3-4 Stage ............................................................................................................................................................................................................ 10-11 1 Placing the Specimen
2 Adjusting the X/Y-Axis Knob Tension
3 Rotating the Stage
3-5 Observation Tube ...................................................................................................................................................................... 12-13 1 Adjusting the Interpupillar Distance
2 Adjusting the Diopter
3 Using the Eye Shades
4 Using Eyepiece Micrometer Disks
5 Adjusting the Tilt
3-6 Condenser ............................................................................................................................................................................................. 14-15 1 Centering the Condenser
3-7 Marker (U-MARKER).......................................................................................................................................................................... 15
4
TRANSMITTED LIGHT SIMPLIFIED POLARIZED LIGHT OBSERVATION
5
TROUBLESHOOTING GUIDE
6
SPECIFICATIONS
7
OPTICAL CHARACTERISTICS
8
ASSEMBLY — See this section for the replacement of the light bulb. —
16
17-19
20-21
22
23-25
PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................. 26-27
IMPORTANT This microscope employs a UIS (Universal Infinity System) optical design, and should be used only with UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series are also usable. For details, please consult Olympus or the catalogues.)
SAFETY PRECAUTIONS
@
Fig. 1
³
²
Fig. 2
1
1. Install the microscope on a sturdy, level table or bench so as not to block the air vents on the underside of the base. Do not place the microscope on a soft surface where the microscope may go down, for this blocks the air vents and may cause fire hazard. 2. This microscope uses air cooling based on natural convection. It should therefore be installed so that spaces of 10 cm or more around the microscope’s rear and side panels as well as the heat sink holes on the lamp socket. 3. To avoid potential shock hazards and burns when replacing the light bulb, set the main switch @ to “ ” (OFF) then disconnect the power cord from the wall outlet in advance. Whenever you replace the bulb during use or right after use, allow the lamp socket ² and bulb to cool before touching. Designated halogen bulbs
6V30WHAL (PHILIPS 5761)
# The microscope also incorporate a fuse (this should be replaced by the manufacturer or authorized agent). 4. Make sure to set the voltage selector switch (³ in Fig. 2) to your local voltage. (The switch has been set to 220-240 V before factory shipment.) 5. For the power cords of the AC adapter of the motorized revolving nosepiece and of the microscope frames, always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cords by referring to the chapter “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cords are not used, product safety and performance cannot be guaranteed. 6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety and performance of the equipment. 7. To prevent electrical shock or malfunction, never insert a metallic object in the air vent holes. 8. If the power cord, AC adapter cord or hand switch cord is distributed near the lamp socket, the cord may be melted, leading to an electrical shock hazard. To prevent this, distribute the cords enough apart from the lamp socket. 9. After use, disconnect the plug of the AC adapter power cord from a wall power outlet to ensure safety.
BX45A
Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol
Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment. Indicate the input jack.
Indicates that a DC supply (from the provided AC adapter) should be used.
Indicates the Hand Switch.
l
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warnings Warning engraving and stickers are placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings. Warning engraving position:
Lamp socket (Warning against high temperature)
Warning sticker position:
Microscope rear panel (Warning against high temperature)
If the warning sticker is contaminated or peeled off, consult your local Olympus representative for replacement.
2
1
Getting Ready
Fig. 3
2
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, refer to chapter 6, “SPECIFICATIONS”.) 3. Do not attempt to switch the motorized revolving nosepiece manually. (This will cause malfunction.) 4. The BX45A microscope has been designed for routine inspections and is not suitable for photomicrography. 5. When moving the microscope, carefully carry it by holding it by the handle on the rear panel and the handle and the base (Weight: approx. 14 kg). # Do not hold the microscope by the stage, coarse/fine adjustment knob or observation tune. Otherwise, the microscope may be damaged. 6. The BX45A can be used with only one single intermediate attachment.
Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room. 2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Never attempt to disassemble any part of the microscope. 4. When not using the microscope, set the main switch to “ ” (OFF), make sure that the lamp socket is cool enough and keep the microscope covered with a dust cover.
3
Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. : Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment). # : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).
3
BX45A
1 NOMENCLATURE }If you have not yet assembled the microscope, read chapter 8, “ASSEMBLY” (pages 23 to 25).
Interpupillar distance scale (Page 12) Centering knob (accommodation position)
Allen screwdriver (accommodation position)
Diopter adjustment ring (Page 12) Main switch
Slider insertion slot
Pilot LED
Motorized revolving nosepiece
Light intensity preset switch (Page 7)
Specimen holder (Page 10)
Light intensity preset adjustment screw (Page 7) Aperture iris diaphragm knob (Page 15)
Brightness adjustment knob (Page 7) (Lamp voltage adjustment system)
Hand switch Filter slider (Page 7) Objective switching button (Page 10)
AC adapter C-6AC
Field iris diaphragm dial (Page 14) X-axis knob (Page 11) Coarse adjustment tension adjustment ring (Page 8)
Y-axis knob (Page 11)
4
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE }Make sure that the AC adapter @, hand switch ² and power cords are connected properly. }Perform the objective confocality adjustment and centering (page 9) before proceeding to the following.
(Controls Used)
Set the main switch to “ I ” (ON).
³Main switch
Engage a low-power objective in the light path.
Place the specimen on the stage.
Bring the specimen in focus.
Adjust the brightness.
Adjust the interpupillary distance. Adjust the diopter. Adjust the optical axis.
Adjust the aperture iris diaphragm and field iris diaphragm.
Engage a high-power objective in the light path.
Insert the required filter.
Adjust the brightness.
Start observation.
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(Page)
|Objective switching button
(P. 10)
ƒSpecimen holder …X/Y-axis knobs
(P. 10) (P. 11)
†Coarse/fine adjustment knobs
(P. 8)
‡Brightness adjustment knob
(P. 7)
ŠBinocular tube ‰Diopter adjustment ring ‹Condenser height adjustment lever ŒCondenser centering screw
(P. 12) (P. 14) (P. 14)
™Aperture iris diaphragm knob šField iris diaphragm dial
(P. 15) (P. 14)
|Objective switching button
(P. 10)
›Filter
(P. 7)
‡Brightness adjustment knob
(P. 7)
BX45A
‰ Centering knobs
Š ³
ƒ
Also used for Πcentering knobs
… @ ‡
š
›
|
²
†
™ ‹ } Make a photocopy of the observation procedure pages and post it near your microscope.
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USING THE CONTROLS 3-1 Microscope Base 1
Voltage Indication
(Fig. 4)
1. Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter. 2. The numerals ² around the knob indicate the approximate voltage.
@
² Fig. 4
2
(Fig. 5)
}The light intensity preset switch @ makes it possible to limit the light intensity to a preselected level regardless of the position of the brightness adjustment knob. The light intensity preset switch has been preset before factory shipment to about 4 V. 1. Press the light intensity preset switch @ to the ON position. (The switch lights when it is ON.) 2. Using a small flat-blade screwdriver, turn the preset adjustment screw ² to obtain the required light intensity. Turning the screw clockwise increases brightness. 3. When the light intensity preset switch is set to OFF, the brightness returns to the level set by the brightness adjustment knob. }While the light intensity preset switch is ON, turning the brightness adjustment knob does not affect brightness.
@ ²
Fig. 5
3
@
Using the Light Intensity Preset Switch
Using the Filter
(Fig. 6)
}Insert only one 32 mm filter @ in the filter slider ² then engage the filter slider in the light path. Usable Filters and Applications
²
32LND6
Light intensity adjustment filter which adjusts the brightness of the light source. (Transmittance: 6%)
32LND25
Light intensity adjustment filter which adjusts the brightness of the light source. (Transmittance: 25%)
32C (provided)/32LBD
Daylight filter/color temperature conversion filter.
32IF550
Green filter which enhances contrast in monochrome photographs.
Fig. 6
7
BX45A
3-2 Focusing Block # The BX45A microscope features a low stage height. The user is recommended to take care so that the user’s hand or finger does not interfere with the stage when turning the coarse adjustment knob.
1
ƒ
² Fig. 7
2
Fig. 8
3
Fig. 9
Adjusting the Coarse Adjustment Knob Tension
(Fig. 8)
# The rotation tension of the coarse adjustment knob should be adjusted using the tension adjustment ring. The coarse adjustment knob tension is preadjusted for easy use. However, if desired, you can change the tension using the tension adjustment ring @. Turning the ring clockwise in the direction of the arrow increases tension, and vice versa. The tension is too low if the objective drops by itself or focus is quickly lost after adjustment with the fine adjustment knob. In this case, turn the ring in the direction of the arrow to increase tension.
@
@
(Fig. 7)
}The fine adjustment knob has been attached on the right side at the factory. }The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the X/Y-axis knobs. Usually attach the knob on the opposite side to the X/Y-axis knobs. 1. Remove the rubber cap @ by puling it out. 2. Loosen the clamping screw ² and remove the fine adjustment knob ³. 3. Remove the seal from the fine adjustment knob screw hole on the other side and attach the new knob by reversing the removal procedure. 4. Attach one of the provided seals on the screw hole ƒ of the fine adjustment dial ³, from which the fine adjustment knob has been removed. }The fine adjustment dial | can be operated with your fingertip or finger surface.
@
| ³
Replacing the Fine Adjustment Knob
Pre-focusing Lever
(Fig. 9)
}The pre-focusing lever ensures that the objective does not come in contact with the specimen and simplifies focusing. After focusing on the specimen with the coarse adjustment knob, turn this lever @ in the direction of the arrow and lock; the lower limit of coarse adjustment movement is set at the locked position. After changing a specimen, refocusing is easily accomplished by rotating the coarse adjustment knob to reach the pre-focused position, then making fine adjustments with the fine adjustment knob. }The objective’s vertical movement activated by the fine adjustment knob is not locked. # If you want to lower the objective to the lower limit, release the lock in advance.
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3-3 Motorized Revolving Nosepiece 1
Adjusting the Confocality of Objective
(Fig. 10 & 11)
}To maintain the accurate focusing even after the objective is switched, the height of the objective with the higher focal point is corrected by attaching a washer. Fifteen washers with three kinds of thickness (10, 30 and 50 µm), three per kind, are provided with the microscope. 1. Engage the objective on the right in the light path and adjust focusing. For use in the confocality adjustment, read the scale indication of the fine adjustment knob (1 scale graduation: 1 µm). 2. Engage the objective on the left in the light path by operating the objective switching button on the hand switch. Adjust focusing, read the scale indication of the fine adjustment knob and obtain the difference from the value read in step 1 above. 3. The objective to use the washer is determined by the rotation direction of the fine adjustment knob @. (Fig. 10) · Direction of the arrow: Objective on the left. · Opposite direction of the arrow: Objective on the right. 4. Remove the objective ², fit the washer | with the required thickness on the screw ³, then attach the objective again. 5. Switch the objective and confirm that confocality is implemented. # Depending on the objective, perfect confocality may sometimes not be achieved.
@ Fig. 10
³ | ²
Fig. 11
2
@ Fig. 12
9
Centering the Objective
(Fig. 12)
}Use the 2 centering knobs @, which are also used in centering the condenser. The centering mechanism is provided only for the objective on the right. 1. Adjust focusing using the objective on the left, then move the target region in the specimen on the center of the field. 2. Using the objective switching button on the hand switch, switch the objective to that on the right. 3. Insert the 2 centering knobs @ into the threaded holes and turn them to move the target in the objective on the center. }After completing centering, store the centering knobs in the accommodation position on the right side panel of the microscope (page 4) so as not to lose them.
BX45A
3 @
Switching the Objective
(Fig. 13)
Each press of the objective switching button @ on the hand switch engages the left and right objective alternately. How to Reset Error Which May Occur During Switching }If the objective hits the specimen or cannot be switched in a normal period of time due to a foreign object, the protection circuitry is activated to abort the switching operation. To release from this condition, set the main switch of the microscope to “ ” (OFF) then “ I ” (ON) again. Then press the objective switching button on the hand switch; the objective on the right will be engaged in the light path.
Fig. 13
3-4 Stage 1
² @
Placing the Specimen # The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.4 mm, and the cover glass should have thickness of 0.17 mm. # When observing very large specimens, remove the specimen holder and place the specimen directly on the stage. Microscopy with Double-Specimen Holder (Fig. 14)
Fig. 14
1. Turn the coarse adjustment knob @ to raise the objective. 2. Open the spring-loaded curved finger ² on the specimen holder and place the specimen on the stage from the front. 3. After placing the specimen as far as it will go, gently release the curved finger. Microscopy with Single-Specimen Holder (Fig. 15) The specimen can easily be placed by sliding it into the specimen holder from the front.
Fig. 15
10
2
(Fig. 16)
1. Hold the X-axis knob @ and slide the Y-axis knob ² up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it. # If the tension is adjusted too tight, a creaking sound may be heard during stage travel, and the stage stopping accuracy may be imperiled.
² |
Adjusting the X/Y-Axis Knob Tension
³ @ Fig. 16
After long hours of use, the stage guide may be deviated and the stage travel range may be decreased. However, this is not malfunction and can be corrected easily as described below. [Treatment] X-axis direction: Hold the specimen holder and move the stage guide to the left and right so that it hits the stoppers. Y-axis direction: Hold the upper stage and move it to the front and rear so that it hits the stoppers. CAUTION
Rubber Stage Knob Caps (Optional) }When the X/Y-axis knobs are fitted with the rubber knob caps, the knobs can be adjusted without slipping and fine adjustment is possible by holding the knobs with a very light force. The rubber knob caps also reduces fatigue after long hours of operation. The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness 2 mm) rubber knob caps are available. To attach the knob rubber caps: First fit the larger rubber knob cap to the Y-axis (upper) knob from below it, then fit the smaller rubber knob cap to the X-axis (lower) knob from below it.
3 @
Rotating the Stage
(Fig. 17)
1. Slightly loosen the stage clamping screw @ using the Allen screwdriver. 2. The stage can be rotated both clockwise and counterclockwise. # A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunction. }The angle of rotation varies depending on the knobs. Angle of Rotation Clockwise
Counterclockwise
Right-hand knobs
230°
20°
Left-hand knobs
20°
230°
Fig. 17
11
BX45A
3-5 Observation Tube 1
Adjusting the Interpupillar Distance
(Fig. 18)
While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated.
Fig. 18
2
@
Adjusting the Diopter
(Fig. 19)
1. Looking through the eyepiece without the diopter adjustment ring, rotate the coarse and fine adjustment knobs to bring the specimen into focus. 2. Looking through the eyepiece with the diopter adjustment ring @, turn it to focus on the specimen.
Fig. 19
3
Using the Eye Shades
(Fig. 20)
When Wearing Eyeglasses Use with the eye shades in the normal, folded-down position. This will prevent the eyeglasses from being scratched. When Not Wearing Eyeglasses Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyes. Fig. 20
4 @
²
Using Eyepiece Micrometer Disks
(Fig. 21)
Eyepiece micrometer disk can be inserted into the WH10X-H (or WH10X) eyepieces. Use a 24 mm dia. x 1.5 mm micrometer disk. Following Fig. 21, remove the micrometer mounting frame ² from the eyepiece and place a micrometer disk @ into the mounting frame while facing down the disk surface with the indication. Re-attach the micrometer mounting frame in the original position.
Fig. 21
12
5
Fig. 22
Adjusting the Tilt (with the U-TBI3)
(Fig. 22)
}Adjust the height and tilt angle of the observation tube to obtain the most comfortable viewing position. Holding the binocular section with both hands, raise or lower it to the desired position. # Never attempt to force the binocular section past the upper or lower stop position. Applying excessive force could destroy the limiting mechanism. # When the U-TBI3 is used, part of the peripheries of the field of view may become dark as the aperture iris diaphragm is stopped down to approximately the minimum aperture. # When the U-TBI3 is used in combination with an intermediate tube (U-EPA2), insufficiency in light may occur in the peripheral area of the field of image. U-ETBI (Fig. 23) The U-ETBI is an ergonomic observation tube with normal field, capable of tilting adjustment with an erect image. The eyepiece position can be adjusted by 45 mm toward the front and rear.
Fig. 23
13
BX45A
3-6 Condenser 1
(Figs. 24 - 26)
1. Turn the condenser height adjustment lever @ in the direction of the arrow to raise the condenser to its upper limit. 2. Focus on the specimen using a lower-power objective. 3. Rotate the field iris diaphragm dial ² in the direction of the arrow so that the diaphragm image comes inside the field of view. 4. Turn the condenser height adjustment lever @ in the opposite direction of the arrow to focus on the diaphragm image. 5. Insert the condenser centering knobs ³ into the two condenser centering screws and turn the knobs to move the iris diaphragm image to the center of the field of view. 6. Gradually open the field iris diaphragm. The condenser is properly centered if the iris image is centered and inscribed in the field of view. 7. During actual use, open the field diaphragm slightly until its image circumscribes the field of view. }When centering is complete, screw the centering knobs into the storage positions on the right side of the microscope frame. (P. 4)
|
@
Centering the Condenser
Fig. 24
² ³ Fig. 25
Effects of Field Iris Diaphragm (Fig. 26) The field iris diaphragm restricts the diameter of the beam of light entering the objective and thus excludes extraneous light, improving image contrast. The diameter of the field iris should be adjusted for objective power to the extent that it just circumscribes the field of view. }When using a 4X objective, set the field iris diaphragm at maximum.
Fig. 26
14
Aperture Iris Diaphragm (Fig. 27)
Aperture iris diaphragm image
70-80% 30-20%
Objective pupil
Fig. 27
· The aperture iris diaphragm determines the numerical aperture of the illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and also increases the depth of focus. · Since the contrast of microscope specimens is ordinarily low, setting the condenser aperture iris diaphragm to between 70% and 80% of the N.A. of the objective in use is usually recommended. If necessary, adjust the ratio by removing the eyepiece and looking into the eyepiece sleeve while adjusting the aperture iris diaphragm knob (| in Fig. 24) until the image shown in Fig. 27 is seen.
3-7 Marker (U-MARKER)
@
² Fig. 28
15
}By mounting the marker @ on the 10X objective, a desired point can be marked with a one-touch operation. The marker can be manipulated with either the left or right hand according to the positioning of the 10X objective. (For details, refer to the separate instruction manual for the U-MARKER.) }The objective for use in cytodiagnosis should be the combination of the Plan10XCY and Plan series objective or the PlanFl10XCY and PlanFl series objective. # To prevent the pen tip of the marker from drying up, always attach the exclusive cap ² on the pen tip whenever it is not used.
BX45A TRANSMITTED LIGHT SIMPLIFIED POLARIZED LIGHT OBSERVATION }The U-ANT analyzer (or a module incorporating analyzer) and the BX45-PO polarizer are required for polarized light observation.
1
@
Attaching the BX45-PO Polarizer
(Fig. 29)
1. Remove the filter slider @. 2. In the slot, insert the BX45-PO polarizer ² all the way. }The cross-Nicol (dark) position is obtained when the polarizer lever comes right beside the microscope frame.
² Fig. 29
²
2
Attaching the U-ANT Analyzer
(Fig. 30)
1. Remove the rubber cap from the slider insertion slot above the revolving nosepiece. 2. Hold the U-ANT analyzer ² so that the surface with indications face up, and drop the analyzer into the dummy slider @ provided with the BX45PO by alighting their index markings. (The analyzer will be fixed by magnetism.) 3. Insert the dummy slider @ containing the U-ANT into the slider insertion slot and push the slider all the way to engage the analyzer in the light path. }The analyzer can be disengaged from the light path by pulling the slider out by one step.
@ Fig. 30
3
Observation Method 1. Engage the objective in the light path. 2. Turn the polarizer lever slightly so that the field of view is darkest (crossNicol position). 3. Place the specimen on the stage and adjust the focusing. 4. Adjust the field iris diaphragm so that its image circumscribes the field of view. 5. The contrast may sometimes be enhanced by closing the aperture iris diaphragm slightly.
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TROUBLESHOOTING GUIDE Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem
Cause
Remedy
Page
1. Optical System a) Bulb does not light.
Bulb is burned out.
Replace bulb.
24
Power cord is unplugged.
Plug power cord into power outlet.
25
b) Bulb lights but the field of view is Aperture and field iris diaphragm are not Open them to appropriate sizes. dark. wide enough. c) Field of view is obscured or not Objective is not engaged correctly in light Press objective switching button again. evenly illuminated. path. Condenser is not centered.
Center it.
Field iris diaphragm is closed too much. Open it sufficiently. Lamp bulb is not installed correctly. d) Dirt or dust is visible in the field of Dirt/dust on eyepiece. view. Dirt/dust on condenser top lens.
Push halogen bulb terminals all the way into stop position.
14/15 10 14 14 24
Clean thoroughly. 3
Dirt/dust on specimen. e) Visibility of observed image is poor. · Image is not sharp. · Contrast is poor. · Details are poorly visible. · Darkfield observation is poorly visible (with flare tendency)
Objective in use is not designed for UIS Replace with a specified objective for series. UIS series.
22
Aperture iris diaphragm is closed too Open it sufficiently. much.
15
Dirt/dust on extremity of objective.
Clean it thoroughly.
Dirt/dust on specimen.
3
Dirt/dust on condenser top lens.
f ) One side of image is blurred.
Thickness of slide glass or cover glass Replace with slide or cover glass with is inappropriate. optimum thickness.
10
Objective is not correctly engaged in light Press objective switching button again. path.
10
Stage is not installed properly.
–
Detach and re-attach stage.
Specimen is not placed correctly on Place specimen correctly on top surstage. face of stage and fix specimen slide with specimen holder.
10
Objective is not correctly engaged in light Press objective switching button again. path.
10
Condenser is not centered correctly.
Center it correctly.
14
h) Field of view can be brightened only Condenser is not centered correctly. slightly by increasing voltage intensity. Condenser is too low.
Center it correctly.
14
Adjust its height correctly.
14
g) Image looks like flowing.
2. Electrical System a) Bulb intermittently lights and goes Bulb is nearly burnt out. Replace bulb. out. A cord or connector is not properly con- Connect cords and plugs securely. nected.
17
24 25
BX45A
Problem b) Lamp bulb burns out soon after lighting.
Cause
Remedy
The bulb in use is not the specified lamp. Replace with a standard bulb.
c) Brightness cannot be varied with Light intensity preset switch is set to ON. Set it to OFF. brightness adjustment knob. Lamp bulb is not installed. Attach lamp bulb.
d) LEDs do not light. Pilot lamp does not light.
Page 24 7 24
Lamp bulb is burnt out.
Replace lamp bulb.
24
Lamp socket is not connected.
Connect lamp socket.
24
Voltage selector switch is set to 220-240 Set is to 100-120 V position. V.
e) Motorized revolving nosepiece will Connection of AC adapter and/or hand Connect cables correctly. switch cable on connector or power outnot work. let side is incorrect.
1
25
Motorized revolving nosepiece is Reset error by following the error restopped in the middle due to an error. setting instructions.
10
a) Coarse adjustment knob is too Rotation tension adjustment ring is too Loosen ring to an optimum tightness. heavy to rotate. tight.
8
3. Coarse/Fine Adjustment Knobs
Upward/downward coarse adjustment is Release lock of pre-focusing lever. attempted while pre-focusing lever is locked. b) Objective drifts down by itself or fo- Tension adjustment ring is too loose. cus is lost due to slipping of fine adjustment knob during observation.
8
Tighten ring to an optimum tightness. 8
c) Coarse adjustment cannot bring Pre-focusing lever is locked on the up- Release lock of pre-focusing lever. objective low enough. per side.
8
d) Objective hits specimen before it is Specimen is placed upside down. brought into focus.
–
Place specimen correctly.
4. Observation Tube a) Field of view of one eye does not Interpupillary distance is incorrect. match that of the other. Diopter adjustment is incorrect.
Adjust interpupillary distance.
12
Adjust diopter.
12
Different eyepieces are used on left and Change on eyepiece to match the right. other so that both sides are the same type.
–
You are not accustomed to parallel opti- When looking into eyepieces, do not cal axis. stare at image from the beginning but see the overall field of view. It is sometimes recommended to turn your eyes away from eyepieces, look far off and look into eyepieces again.
–
5. Stage a) Image shifts when you touch stage. Stage is not properly mounted.
Clamp stage.
11
b) X-axis traverse stops midway.
Place specimen correctly.
10
Specimen is not correctly positioned.
c) X-axis and/or Y-axis knobs too tight Tension of X-axis and/or Y-axis knobs too Adjust tension. or too loose. high or too low.
11
18
Problem d) Stroke has reduced.
Cause
Remedy
Page
Stage guide is deviated.
Use treatment method described on page 11.
11
Pen has run out of ink.
Replace pen.
–
Stroke adjustment is incorrect.
Re-adjust stroke.
–
Pen tip is dry.
Wipe pen tip with tissue paper and retry writing.
–
6. Marker a) Marking is impossible.
19
b) Slide glass slips out as a result of Stroke adjustment is incorrect. marking.
Re-adjust stroke.
c) Pen service life is very short.
Cap pen whenever possible.
Pen tip is dry.
– –
BX45A
SPECIFICATIONS Item
Specification
1. Optical system
UIS (Universal Infinity System) optical system
2. Illumination system
Transmitted Koehler illuminator built in. 6 V, 30 W halogen bulb (pre-centered) 6V30WHAL (PHILIPS 5761). (Average life time: Approximately 100 hr. when used as directed) Intensity control range: Below 2 V to 5.9 V (continuously variable) Light preset switch: Preset voltage can be set arbitrarily between below 2 and 5.9 V. Rated input voltage: 100-120 V/220-240 V , 0.8 A/0.4 A, 50/60 Hz. Power consumption: 45 W.
3. Focusing system
Revolving nosepiece height movement by roller guide (rack & pinion) Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse) Full stroke range: 15 mm Coarse adjustment pre-focusing lever Rotation tension adjustment on coarse focus adjustment knob.
4. Motorized revolving nose- 2-position lateral swinging system (Rotation angle: 62°) piece Centering available for objective hole on the right. Operation by objective switching button on the hand switch. Mechanism for shock reduction in objective switching. Protection circuit (The motor is released in 3 sec. after penetration of a foreign object.) 5. AC adapter
Rated input voltage: 100-120 V/220-240 V
6. Observation tube
Model
, 1.0 A/0.5 A
U-BI30-2
U-TBI3
Widefield tilting binocu- Widefield erected-image lar tilting binocular
Widefield binocular Field number Tube tilting angle
FN 22 5°- 35° (Continuously variable)
30°
I n t e r p u p i l l a ry distance adjustment 7. Stage
Model
U-ETBI
0°-25° (Continuously variable)
50 to 76 mm U-SVRC
U-AVLC
Coaxial bottom right knob
Coaxial bottom left knob
Rectangular ceramic-coated wire driven stage Size
156 mm (Depth) x 191 mm (Width)
Cross moveX/Y-axis knob tension adjustable. ment mechaMovement range: X-axis 76 mm, Y-axis 52 mm nism Specimen holders (for singlespecimen observation)
U-HLS4 U-HLST4
U-HRS4 U-HRST4
Specimen holders (for doublespecimen observation)
U-HLD4 U-HLDT4
U-HRD4 U-HRDT4
20
Item
21
Specification
8. Condenser
Abbe condenser, fixed. NA: 0.1 to 0.9 Aperture iris diaphragm: Aperture iris diaphragm scale provided. Applicable objective magnifications: 4X to 40X
9. Operating environment
· · · ·
Indoor use. Altitude: Max. 2000 meters Ambient temperature: 5° to 40°C (41° to 104° F) Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F). · Supply voltage fluctuations: ±10% · Pollution degree: 2 (in accordance with IEC664) · Installation (overvoltage) category: II (in accordance with IEC664)
BX45A
OPTICAL CHARACTERISTICS The table below shows the optical characteristics of different eyepiece and objective combinations. Objective specifications are marked on the objective (as shown in the diagram on the right).
Name
Magnification
Numerical aperture
NOTE Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces and objectives that can be combined with this microscope. Optical character
Magnification
N.A.
4X 10X 40X
0.10 0.25 0.65
Eyepiece
W.D. (mm)
Cover glass thickness
Resolution (µm)
Total mag.
28.90 6.30 0.62
— 0.17 0.17
3.4 1.34 0.52
40X 100X 400X
Objective AchC Achromat* (FN20)
Cover glass thickness
Mechanical tube length
WHC10X (FN20) Depth of focus (µm)
Remark Field of view
175 28.0 3.0
5.0 2.0 0.5
* Be sure to use the AchC series in combination with the WHC10X. Indicates the objective is provided with a correction collar. Optical character
W.D. (mm)
Resolution (µm)
UPlanFl Universal Plan Apochromat (FN26.5) UPlanApo Universal Plan Apochromat (FN26.5) PlanApo Plan Apochromat (FN26.5) UMPlanFl No Cover Universal Plan Semi-Apochromat (FN26.5) PlanFl Plan Semi-Apochromat (FN26.5)
WH10X (FN22) Depth Total of focus Field mag. of view (µm)
Magnification
N.A.
10X 20X 40X 4X 10X 10XCY 20X 40X 4X 10X 20X 40X 4X 10X 20X 40X
0.25 0.40 0.65 0.10 0.25 0.25 0.40 0.65 0.13 0.30 0.50 0.75 0.16 0.40 0.70 0.85
6.1 3.0 0.45 22.0 10.5 10.5 1.2 0.56 17.0 10.0 1.6 0.51 13.0 3.1 0.65 0.2
— — 0.17 — — — 0.17 0.17 — — 0.17 0.17 — 0.17 0.17 0.11-0.23
1.34 0.84 0.52 3.36 1.34 1.34 0.84 0.52 2.58 1.12 0.67 0.45 2.1 0.84 0.48 0.39
100X 200X 400X 40X 100X 100X 200X 400X 40X 100X 200X 400X 40X 100X 200X 400X
28.0 6.09 3.04 175 28.0 28.0 6.09 3.04 127 22.4 7.00 2.52 99.5 15.9 4.65 2.14
1.25X 2X
0.04 0.08
5.1 6.0
— —
8.38 4.19
12.5X 20X
872 398
40X
0.75
0.63
0
0.45
400X
10XCY
0.30
9.90
—
1.12
100X
Objective Ach Achromat (FN22) Plan Plan Achromat (FN22)
Eyepiece
Cover glass thickness
1.66
22.0
2.2 1.1 0.55 5.5 2.2 2.2 1.1 0.55 5.5 2.2 1.1 0.55 5.5 2.2 1.1 0.55 17.6 11.0
WH15X (FN14) Total mag. 150X 300X 600X 60X 150X 150X 300X 600X 60X 150X 300X 600X 60X 150X 300X 600X 18.75X 30X
0.55
600X
2.2
150X
Remark Depth Field of focus of view (µm) 20.9 1.4 4.64 0.7 2.35 0.35 85.8 3.5 20.9 1.4 20.9 1.4 ND filter 4.64 0.7 2.35 0.35 92.9 3.5 16.5 1.4 5.22 0.7 1.93 0.35 71.7 3.5 11.5 1.4 3.39 0.7 1.62 0.35 639 287
1.27
16.4
11.2 7.0
0.35
1.4
ND filter
22
ASSEMBLY 8-1 Assembly Diagram The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly. The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. # When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass surfaces. Assembly steps enclosed in will be detailed on the subsequent pages. }All assembly operations are possible by using the Allen screwdriver ( ) provided with the microscope.
Eyepiece WH10X
Observation tube U-BI30-2 U-TBI3 U-TR30-2 U-ETBI Intermediate attachment U-EPA2 U-DA, etc.
Hand switch
Specimen holder U-HLD-4 U-HRD-4 U-HLS-4 U-HRS-4
UIS objective Plan10XCY PlanFI10XCY
AC adapter UIS objective series
Marker U-MARKER
Tube clamping screw Halogen bulb 6V30WHAL
Specimen holder clamping knob
Power cord
Lamp socket U-LS30-4 Stage clamping screw Stage U-SVRC U-SVLC U-SRG2
Power cord
Microscope frame BX45ATF
23
BX45A
8-2 Detailed Assembly Procedures @
1
Attaching the Lamp Socket
(Fig. 31 & 32)
The applicable bulb is the 6V30WHAL (PHILIPS 5761) halogen bulb. 1. Hold the bulb @ by wrapping it with a piece of gauze, and push the bulb pins ² all the way into the pin holes ³ of the lamp socket. # To prevent reduced bulb life or cracking, do not touch the bulb with bare hands. If fingerprints are accidentally left on the bulb, wipe the bulb with a soft cloth.
² ³ Fig. 31
ƒ
|
2. Align the guide pins | with the guide holes ƒ on the rear of the microscope frame, then push the plug … all the way into the socket † so that the guide pins fit in the guide holes. Caution for Bulb Replacement During After Use The bulb, lamp socket and areas near these will be extremely hot during and right after use. Set the main switch to “ ” (OFF), disconnect the power cord from the wall outlet, then allow the old bulb and lamp socket to cool before replacing the bulb with a new one of the designated type.
† … Fig. 32
5
Attaching the Objective
(Fig. 33)
Screw in a lower-power objective and higher-power firmly into the motorized revolving nosepiece. }When switching a 10X and 40X objectives alternately during observation, it is recommended to use the PlanFI10XCY or Plan10XCY because these objectives incorporate built-in ND filters so it is not required to adjust the brightness when the objective is switched to the 40X, thereby improving the ease of operation. Inserting the ND filter for Plan10X objective
² @
}The ND filter (12.6ND25) for the Plan10X objective provides the objective with the same characteristics as the CY specification. 1. Drop in the ND filter @ in the 10X objective. (Fig. 33) 2. To fix the ND filter, drop in the ring spring ² provided with the filter by narrowing the ring. (Fig. 33)
Fig. 33
24
@
7
²
ƒ …
| ³
‡
† Fig. 34
Š
Fig. 35
25
‰
Attaching the Cables and Cords
(Fig. 34 & 35)
Always use the AC adapter and its power cord provided by Olympus. If no power cord is provided with the microscope, please select the proper power cord by referring to chapter “PROPER SELECTION OF THE POWER SUPPLY CORD” on next page. The cables and cords are vulnerable when bent or twisted. Never subject them to excessive force. Make sure that the main switch of the BX45ATF is set to “ ” (OFF) before connecting cables and cords. 1. Insert the hand switch plug @ into the HS connector ² on the rear of the microscope. }The hand switch is provided with double-side adhesive tape. Use it when you want to install the hand switch in an easy-to-operate position. 2. Insert the AC adapter’s power output jack ³ into the microscope’s power input jack |. 3. Insert the power cord’s connector … into the AC adapter’s power input connector ƒ, then insert the plug Š of the AC adapter power cord into a wall power outlet ‰. The power cord should be connected to a grounded, 3-conductor power outlet. If the power outlet is not grounded properly, Olympus can no longer warrant the electrical safety performance of the equipment. 4. Insert the power cord’s connector † into the microscope’s power input connector ‡. 5. Insert the plug Š of the microscope power cord into a wall power outlet ‰. If a power cord, AC adapter cord or hand switch code comes in contact with the lamp socket or the surroundings, the cord may melt down, causing electric shock hazards. Be sure to distribute the cords at enough distance from the lamp socket.
BX45A
PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
Specifications Voltage Rating Current Rating Temperature Rating Length Fittings Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum 3.05 m maximum Grounding type attachment pulg cap. Opposite terminates in molded-on IEC configuration appliance coupling.
Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the agencies mentioned in Table 1, please use replacements approved by any othere quivalent and authorized agencies in your country.
Country
Agency
Certification Mark
Country
Agency
Argentina
IRAM
Italy
IMQ
Australia
SAA
Japan
MITI
Austria
ÖVE
Netherlands
KEMA
Belgium
CEBEC
Norway
NEMKO
Canada
CSA
Spain
AEE
Denmark
DEMKO
Sweden
SEMKO
Finland
FEI
Switzerland
SEV
France
UTE
United Kingdom
ASTA BSI
Germany
VDE
U.S.A.
UL
Ireland
NSAI
Certification Mark
26
Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Approval Organization
Printed or embossed Harmoniza- Alternative Marking Utilizing tion Marking (May be located on Black-Red-Yellow Thread (Length jacket or insulation of internal wir- of color section in mm) ing) Black Red Yellow
Comite Electrotechnique Belge (CEBEC)
CEVEC
10
30
10
Verband Deutscher Elektrotechniker (VDE) e.V. Prüfstelle
30
10
10
Union Technique de d´Electricite´ (UTE)
USE
30
30
10
Instituto Italiano del Marchio di Qualita´ (IMQ)
IEMMEQU
10
30
50
British Approvals Service for Electric Cables (BASEC)
BASEC
10
10
30
N.V. KEMA
KEMA-KEUR
10
30
30
SEMKO AB Svenska Elektriska Materielkontorollanstalter
SEMKO
10
10
50
Österreichischer Verband für Elektrotechnik (ÖVK)
<ÖVE>
30
10
50
Danmarks Elektriske Materielkontrol (DEMKO)
30
10
30
National Standards Authority of Ireland (NSAI)
30
30
50
Norges Elektriske Materiellkontroll (NEMKO)
NEMKO
10
10
70
Asociacion Electrotecnica Y Electronica Espanola (AEE)
30
10
70
Hellenic Organization for Standardization (ELOT)
ELOT
30
30
70
Instituto Portugues da Qualidade (IPQ)
l np l
10
10
90
Schweizerischer Elektro Technischer Verein (SEV)
SEV
10
30
90
Elektriska Inspektoratet
SETI
10
30
90
Underwriters Laboratories Inc. (UL) Canadian Standards Association (CSA)
SV, SVT, SJ or SJT, 3 X 18AWG SV, SVT, SJ or SJT, 3 X 18AWG
This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic compatibility and directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with the above directives.
27
2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia
This publication is printed on recycled paper. Printed in Japan 2000 08 M 005–@
