Transcript
INSTRUCTIONS
BX40
SYSTEM MICROSCOPE
This instruction obtain Optimum we recommend this instruction
manual is for the Olympus System Microscope Model BX40. To ensure the safety, Performance, and to familiarize yourself fully with the use of this microscope, that you study this manual thoroughly before operating the microscope. Retain manual in an easily accessible place near the work desk for future reference.
Illlllll Illllllll lllll Ill1 IIIIllIllll lllll lllll Ill1 AX7125
This unit employs a UIS (universal infinity System) with UIS eyepieces, objectives, and condensers. result if inappropriate accessories are used.
Optical design, and should be used only Less than Optimum Performance may
1. Install the microscope on a sturdy, level table or bench. The air vents on the underside of the base should never be blocked such as by placing the microscope on a flexible surface, as this could result in overheating and Cause a fire. 2. To prevent obstruction of the natura1 convection of this microscope, make sure to leave at least 10 cm of free space between Walls, or other objects, and the left, right and rear sides of the microscope and the lamp socket when installing this microscope. 3. To avoid potential shock hazards and burns, first set the main switch 0 to “0” (OFF) and then disconnect the power cord from the wall outlet before replacing the bulb. Whenever you replace the bulb during use or right after use, allow the lamp socket @ and bulb to cool before touching.
Designated bulb
6V 30WHAL-L (PHILIPS 5761)
4. Make Sure that the voltage selector switch @ located on the rear side of the microscope frame is set to conform with the local mains voltage. (When shipped from the factory, the switch is set to 220-240V.) 5. Always use the power cord provided by Olympus. If no provided, please select the proper power cord by referring “PROPER SELECTION OF THE POWER SUPPLY CORD” this instruction manual. If the proper power cord is not used, and Performance cannot be guaranteed.
power cord is to the section at the end of product safety
6. Always ensure that the ground terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus tan not longer Warrant the electrical safety and Performance of the equipment. 7. Never insert metal objects, etc. into the air vents of the microscope frame as this could result in electrical shock and personal injury.
) Safety Symbols
)
The following Symbols are found on the microscope. Study the meaning of the Symbols, and always use the equipment in the safest possible manner.
Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or darnage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warning engravings/stickers are placed at Parts where special precaution is required when handling and using the microscope. Always heed the warnings. Warning engraving Position
Lamp socket
[Warning against high temperaturel
Warning sticker Position
Microscope frame rear Panel
[Warning against high temperaturel
Should warning stickers become soiled, peel off, etc. contact Olympus for replacements.
a
GetZing Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. DO not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. For operating conditions, refer to Section 6, “SPECIFICATIONS” on page 21. 3. When moving the microscope, carefully carry it with both hands by grasping the arm as shown in the figure on the left. (Weight: approximately 12.5 kg (28 Ib.) J, Darnage to the microscope may occur if you grasp it by the Stage, coarse adjustment knob, or binocular section of the Observation tu be, etc. 4. The 5x40 tan be used with up to two intermediate attachments (e.g., a U-CA magnification changer and/or U-EPA eyepoint adjuster). For restrictions when using additional intermediate attachments, make sure to read the instruction manual provided with the respective intermediate attachments.
a.,
Maintenance
and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints slightly moistened with a mixture of ether (70%) and alcohol (30%).
or oil smudges,
wipe with gauze
A
Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical Sparks - for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a weil-ventilated room.
2. DO not attempt to use organic solvents to clean the non-Optical components of the microscope (especially plastic Parts). To clean these, use a lint-free, soft cloth lightly moistened with a diluted neutral detergent. 3. DO not disassembly
any part of the microscope as this could result in malfunctions or reduced Performance
4. When not using the microscope,
keep it covered with the provided dust cover.
’Caution If the attachment is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual
The following Symbols are used to set off text in this instruction manual. A
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or darnage to equipment (including objects in the vicinity of the equipment). * : Indicates that failure to follow the instructions could result in darnage to equipment. 0 : Indicates commentary (for ease of Operation and maintenance).
BX40
a
2-l
Assembly
Diagram ........................................................................
2
2-2
Assembly
Procedure
.....................................................................
3
5-1
Base .................................................................................................
10
5-2
Stage ...............................................................................................
12
5-3
Observation
5-4
Condenser
5-5
Focusing
5-6
Immersion
5-7
Photomicrography
1
Tube .......................................................................... ....................................................................................... Adjustment
Knobs
Objectives
........................................................
14 16 18
...................................................................
19
.........................................................................
19
n PROPER SELECTION OF THE POWER SUPPLY CORD . .. . .. .. . .. . .. . .. .. . .. .. . .. 26
1 Eyepiece
__/______
Revolving
1 Observation
Tube
Nosepiece
Lamr, Socket
~
1
1
0
The diagram below Shows the sequence of assembly of the various modules. The numbers in&& *
When assembling the microscope, Parts or touching glass sutfaces.
the Order of
make Sure that all Parts are free of dust and die, and avoid
scratching
assembly,
_
any
k
!
Eyepiece
Observation
tube Allen screwdriver
Halogen bulb
Microscope
frame
cord
Condenser
0
2
a
USe
Onlya
designated bulb 6V, SOWHAL halogen bulb (PHILIPS 5761). (
1. Holding the bulb @ with gloves or a piece of gauze, insert the bulb pins @ fully into the pin holes 0. (Fig. 1) * To prevent reduced bulb life or cracking, do not tauch the bulb with bare hands. If fingerprints are accidentally left on the bulb, wipe the bulb with a soft cloth. Fig. 1
2. Aligning the guide pins @ with the guide pin holes @ at the rear of the microscope frame, and the plug @ with the socket Q), gently push the lamp socket into place (Fig. 2) A
Fig. 2
Bulb Replacement During Use or Right After Use The bulb and the lamp socket and areas near these will be extremely hot during use and right after use. Press the main switch to “0” (OFF) and disconnect the power cord from the Wall outlet. Then allow the old bulb and lamp socket to cool before replacing the bulb with a new of the designated type.
1. Loosen the clamping screw @ at the front of the Stage. 2. Gently lower the Stage onto the substage dovetail @ and then tighten the clamping screw (iJ
Fig. 3
l l
3
0
a
Fig. 4
the Slide Holder
(Fig. 4)
1. Loosen the slide holder clamping screws (iJ to the extent that they just clear the thickness of the slider holder. * If the clamping screws are loosened too much, their threaded portions may interfere with the Slots in the slide holder. 2. Insert the slide holder from the front until it Comes up against the stop. At this Point, make sure the slide holder clamping screws fit into the positioning Slots on the specimen holder. * If the slide holder is not fully inserted, it may attach in a tilted Position. 3. Tighten the slide holder clamping screws.
a1
Fig. 5
Attaching
Mounting
the Condenser
(Fig. 5)
1. Turn the coarse adjustment knob Q) to raise the Stage to its upper limit. 2. Turn the condenser height adjustment knob @ to lower the condenser holder to its lowest Position. 3. Loosen the condenser clamping screw 0. 4. Position the condenser so that the aperture scale is located in front. Gently slide the condenser along the dovetail. 5. Tighten the condenser clamping screw and gently raise the condenser holder to its highest Position. t When mounting the U-SC Swing-out condenser, align the positioning pin at the back of the condenser with the groove in the condenser sleeve. * When using the U-SC Swing-out condenser or the U-UCDIUCDB universal condenser, Swing the top lens out of the way before inserting the condenser.
a
Mounting
the Objectivec;
(Fig. 61
1. Turn the coarse adjustment knob to lower the Stage to its lowest position. 2. Mount the objectives on the revolving nosepiece in such a manner that the magnification increases from low to higher in a clockwise direction. Fig. 6
a.
Mounting
the Observation
Tube
(Fig. 7)
1. Using the Allen screwdriver, loosen the Observation tube clamping screw
Fig. 7
0
0. 2. Insert the circular dovetail mount at the bottom of the Observation tube into the opening on the microscope frame, placing the Observation tube to Point the binocular eyepieces towards the observer. Clamp the Observation tube by tightening the clamping screw. Ir If the direction of Stage movement and the direction of image movement differs during observations, loosen the Stage clamping screw slightly and adjust the Stage by rotating it, while obser-ving the image.
4
1. Remove the eyepiece sleeve dust taps (iJ. 2. Insert the eyepieces @ into the eyepiece sleeves as far as they will go. (Fig. 8) Ir When using the binocular tube, helicoid equipped focusing eyepieces cannot be used.
Fig. 8
When using the trinocular Observation tube (U-TRBO) or super widefield trinocular Observation tube (U-SWTR) When using a finder eyepiece or an eyepiece with micrometer adjustment, insert this eyepiece into the right-hand eyepiece sleeve. When doing so, make Sure that the eyepiece positioning pin @ fits into the notch @ on the eyepiece sleeve. (Fig. 9) QThe super-widefield trinocular Observation tube is equipped with a positioning notch on both eyepiece sleeves. Make sure that the positioning pin on both eyepieces fits into the respective notch. Fig. 9
A A A
Fig. 10
The power Cord is vulnerable when bent or twisted. Never subject it to excessive forte. Make Sure that the main switch @ is on “0” (OFF) before connecting the power Cord. (Fig. IO) Always use the power Cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual.
1. Connect the power Cord plug @ to the AC receptacle 0. (Fig. 10) 2. Plug the power Cord plug @ into a Wall outlet. (Fig. 11) A
Connect the power cord correctly and ensure that the ground terminal of the power supply and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus tan no longer Warrant the electrical safety and Performance of the equipment.
Fig. 11
5
0
BX40
Interpupiliary
distance
adjustment
scale
/ Allen screwdriver
Condenser height adjustment knob
Diopter
adjustment
ring
Slide holder
Voltage indicator scale
Condenser aperture iris diaphragm ring Light intensity lever
Y-axis knob
/
X-axis knob
Pre-focusina
/
Light intensity preset adjustment screw Light intensity preset button
lever
Field iris diaphragm
ring
Filter mount
l
6
0 0
\ Il;,e
7
selector
0
1. Press the main switch to ” 1” (ON) and adjust the brightness with the light intensity adjustment lever. (At this Point, keep the light intensity preset button OFF.) (p. IO)
2. Disengage all filters from the light path. (p. IO)
3. Turn the revolving nosepiece to engage the 1OX objective. Make sure the revolving nosepiece clicks into Position.
m
1 z : 2
l
I
4. Place a specimen on the Stage. (p. 12)
5. Turn the X-axis knob @ and the Y-axis knob @ to move the specimen into the light path. 0 Using a long Stage knob is convenient for clinical inspection
[Using a trinocular Observation tubel 6. Push the Observation tube’s light path selector knob to “100% for binocular eyepieces” (the pushed-in position). (p. 15)
7. Looking through the right eyepiece with your right eye, turn the coarse adjustment knob to bring the specimen into focus. After obtaining approximate focus, use the fine adjustment knob to make fine adjustments. (p. 18)
0
8
8. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring to focus the specimen. (p. 14)
9. Adjust the interpupillary
distance of the eyepieces.
(p. 14)
10. Adjust condenser centration and focus. (p. 16)
11. Engage the objective to be used and readjust the light intensity to the desired level for Observation, then readjust the focus. 12. Engage your choice of filters in the light path. (pp. IO,1 1)
13. Adjust the field iris diaphragm. (p. 16)
14. Adjust the aperture iris diaphragm. (p. 17)
9
0
BX40
-
Valtage
a
hdicator
(Fig. 12)
1. Sliding the light intensity adjustment lever @ upward increases the voltage, making illumination brighter. 2. The numerals to the right of the voltage indicator LEDs @ indicate the voltage.
Fig. 12
‘Engaging the Light
Fig. 13
-
Using
the Light Intensity
k&!nSity
hZSE!t
&utton (Fig. 131
The light intensity preset button @ makes it possible to limit the light intensity to a preselected level regardless of the Position of the light intensity adjustment lever. 1. Push the light intensity preset button @ to the ON Position. (The face of the button lights when the button is ON.) 2. Using a small screwdriver, turn the preset adjustment screw @ to obtain the required light intensity. Turning the screw clockwise increases brightness. 3. Switch the light intensity preset button OFF and brightness returns to the level set by the light intensity adjustment lever. * Moving the light intensity lever does not affect brightness while the light intensity preset button is ON
SJ z Cl 2 8 5 8 L
Preset Button
The light intensity preset button allows you to temporarily adjust brightness to a preset level for applications such as photomicrography, making it unnecessary to manually adjust the brightness each time you take a Photograph. 0 Before shipment from factory, the preset level is set to an intensity suggested for photomicrography. OThe light intensity preset button is also useful when using two different objectives alternatingly, helping you to avoid manually adjusting the brightness each time you Change magnification.
Accessory
Fig. 14
0
Filter
(Fig. 14)
1
You tan place a 45 mm diameter filter in the filter holder on the light exit at the base of the microscope. If you need to use two or more filters at once, use a filter cassette. Ir When using a filter cassette, you tan additionally use a Single filter with a thickness of less than 3 mm over the light exit glass. * When using interference filters, such as 45LBD-IF, in the filter holder over the light exit glass, flares may become conspicuous. lf this occurs, slightly tilt the filter.
IO
Using the Filter Cassette
(Figs. 15-18)
If you need to place 3 or more filters over the light exit window, a filter cassette.
Loading
1
use
Filters Into the Filter Cassette (Fig. 15)
0 The filter cassette accommodates filters with a diameter of 45 mm and a thickness of 2.7 mm or less. 0 The filter cassette has two filter levers on the right side and one on the left side.
Fig. 15
1. Move all filter levers to the OUT Position except for the one belonging to the slot into which the filter is to be inserted. 2. Slide lever Q to the IN Position. Make sure that it clicks securely into place. (Fig. 15) 3. Holding the lever in the Position shown in Fig. 15, put the filter into the cassette by inserting it in the direction indicated by the arrow. 4. Insert the other two filters in the same manner.
Mounting
the Filter Cassette (Figs. 16,17)
1. Fully loosen the filter cassette clamping screw 0. (Fig. 16) 2. Holding the filter cassette above the light exit glass, align the key @ with the slot @ and snap the filter cassette into place from above. 3. Rotate the filter cassette to align its sides with the base. (Fig. 17) 4. Align the clamping screw @ with the positioning hole @ on the light exit, then tighten the screw to fasten the filter cassette. + When the filter cassette is installed, the Stage may hit it when lowered. Therefore, exercise caution when lowering the Stage with the filter cassette installed.
Fig. 16
1 Using the Filter Cassette (Fig. 18)
Fig. 17
8
1
45LBD-IF
Color balancing filter
45ND-6, 45ND-25
Neutral density filter
45G-530, 456-533, 45lF550
Green
45Y-48
Yellow
450-560
Orange
45C-3, 45KB3
Daylight filter
B & W contrast filter
Up to three of the above filters tan be inserted into the filter cassette. Moving the levers @ on the left and right sides of the cassette to the IN Position brings the corresponding filter into the light path.
Fig. 18
11
0
BX40
SpeMnefl
a
Placement
(Figs. 19-21)
+ For Single slide observations, the maximum slide dimensions are 26 x 76 mm, with a thickness of 0.9-1.4 mm and a cover glass thickness of 0.17 mm. + When observing very large specimens, remove the slide holder and place the specimen directly on the Stage. (Fis. 19) j
1 Slide Holder for 2 Soecimens Fig. 19
1. Turn the coarse adjustment knob @ to lower the Stage. 2. Open the spring-loaded curved finger @ on the slide holder and place one or two specimen slides on the Stage from the front. 3. After placing the slides as far as they will go, gently release the curved finger. (Figs. 20, 21)
Slide Holder for Single Specimen
The specimen tan easily be placed by sliding it into the slide holder from the front. (Fig. 20)
5-l = 6 m
Fig. 20 Using an Oil Immersion
Objective
Adsorption of immersion oil tan Cause the specimen to drift. In such cases, it is recommended to use the optional specimen Clip @ (BH2-SCB-3) for oil immersion objectives 0. (Fig. 21)
Fig. 21
Q
Adjusting the Tension and V-axis Knobs
of the X-axis
(Fig. 221
The tension of the X-axis and Y-axis knobs tan be individually adjusted. Turning the X adjustment ring @ or the Y adjustment ring @ counterclockwise increases tension, and turning them clockwise reduces tension. When adjusting the tension, hold the X-axis and Y-axis knobs to keep them from turning along with the tension adjustment rings. + If the tension is adjusted to tight, a creaking Sound may be heard during Stage travel, and the Stage may backtrack. Fig. 22
0
12
1. Slightly loosen the Stage clamping screw 0. 2. The Stage tan be rotated both to the left and to the right by turning it with the Stage clamping screw. Sr A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunction. 0 The angle of rotation varies depending on the Position of the Stage knobs. Fig. 23
Specifications are identical for both the Standard type and long type knobs.
0 By lowering the Position of the substage, the microscope will accommodate specimens with maximum heights of 40 mm. This is useful when observing metallurgical specimens and other thick objects. 1. Lower the Stage to the lower limit, then remove the Stage from the microscope. (See page 3) 2. Using the Allen screwdriver, loosen the substage bracket clamping screw 0 and remove the substage. (Fig. 24) Fig. 24
3. Turn the coarse adjustment knob and raise the focusing block @ to where the stopper screw @ in the arm becomes visible. (Fig. 25) 4. Using the Allen screwdriver, loosen and remove the upper stopper screw 0. 5. Reattach substage bracket and Stage. 0 Store the removed stopper screw @ in a safe place so that it will not be lost, if needed again.
Fig. 25
13
0
While looking through the eyepieces, adjust for binocular Vision until the left and right fields of view coincide completely. The index dot l indicates the interpupillary distance. 0 Note your interpupillary distance so that it tan be quickly duplicated.
Fig. 26
1, Looking through the right eyepiece with your right eye, rotate the coarse and fine adjustment knobs to bring the specimen into focus. 2. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring (iJ to focus on the specimen. (Fig. 27)
Fig. 27 (
Usinn a Finder Evepiece
1
1, Looking through the right eyepiece with your right eye, turn the top of the eyepiece @ until a clearly defined double crossline tan be seen in the field of view. (Figs. 27, 28) 2. Looking through the right eyepiece, rotate the coarse and fine adjustment knobs to bring the specimen and double crossline into simultaneous focus. 3. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring @ to focus on the specimen. Fig. 28
a
4i3Q. 29)
1 When Wearing
Eyeglasses
Use with the eye shades in their normal folded down Position. This will prevent the eyeglasses from being scratched. 1 When Not Wearinn
Evenlasses
Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces and eyeglasses. Fig. 29
0
14
Eyepiece micrometer discs tan be inserted into WHIOX-H (and WHIOX) eyepieces. Use 24 mm diam. x 1.5 mm micrometer discs. Following Fig. 30, unscrew the micrometer mounting frame @ from the eyepiece and place a micrometer disc @ into the mounting frame. The engraving on the micrometer disc @ should face downward into the micrometer mounting frame 0. Screw the micrometer mounting frame back into the eyepiece tube.
Fig. 30 Slide the light path selector knob @ to select the desired light path. 0 The selector knob is ordinarily at the middle position. With dark specimens, push the knob in. If additional light is needed for television or photomicrography, pull the knob out.
100% for binocular Observation of eyepieces ( darkspecimens
Pushed in
Middle Position Fig. 31
l
Pulled out l
LxlaxQ 1m I
Observation of bright specimens. photography, n/ obsep&ion
20% for binocular eyepieces, 80% for Wphotwwhy
I I
fi
1
l
I
/ 100% for lV/photography
I Photography, / TV observatrons
/
I
l
I
Note: The U-llR is not equipped with a light path selector knob. The intensity ratio of this Observation tube is 20% for binocular eyepieces, 80% for TV/ photography.
Fig. 32
15
0 Adjust the height and tilt of the Observation tube to obtain the most comfortable viewing Position. Holding the binocular section with both hands, raise or lower it to the desired Position. j, Never attempt to forte the binocular section past the upper or lower stop positions. Applying excessive forte could destroy the limiting mechanism. + When using the U-TBI-2 tilting Observation tube, only the viewing attachments U-DO, U-MDO, U-SDO and the eyepoint adjuster UEPA tan be used. However, when using Plan20X (for a general P-level configuration), illumination at the periphery will be slightly insufficient and vignetting in the peripheral field may occur when the apetture iris diaphragm is stopped down near its minimum size. (If intermediate attachment combinations other than the designated are used, illumination at the periphery will be slightly insufficient.) 0 When using the U-TTR tilting Observation tube, the straight Photo tube U-SPT or various Video adapters (with the exception of the double-port U-DPT) tan be mounted on the trinocular Observation tube. * The U-TBI tilting Observation tube is not recommended for use in combination with intermediate attachments.
0
Fig. 33
0 0
1. Turn the condenser height adjustment knob 0 to raise the condenser to its upper limit. (Fig. 33) 2. Focus on the specimen using the IOX objective. * When using the U-SC Swing-out condenser, move the top lens into the light path. 3. Rotate the field iris diaphragm ring @ in the direction of the arrow to where the diameter of the diaphragm is near its smallest. 4. Manipulate the condenser height adjustment knob @ until the iris diaphragm image is Seen in sharp focus within the field of view. 5. Turn the two condenser centering screws @ to move the iris diaphragm image to the Center of the field of view. 6. Gradually open the field iris diaphragm. The condenser is properly centered if the iris image is centered and inscribed in the field of view. 7. During actual use, open the field diaphragm slightly until its image circumscribes the field of view.
5 0
Field Iris Diaphragm
1
The field iris diaphragm restricts the diameter of the beam of light entering the objective and thus excludes extraneous light, improving image contrast. The diameter of the field iris should be adjusted for objective power to the extent that it just circumscribes the field of view. (See “Compatibility of Objectives and Condensers” on the next Page.1
Fig. 34
2 2 0 L
Apetture
Aperture iris diaphragm l
l
Fig. 35
Iris Diaphragm
(Figs. 35.36)
1
The aperture iris diaphragm determines the numerical aperture of the illumination System. Matthing the numerical aperture of the illumination System with that of the objective provides better image resolution and contrast, and also increases the depth of focus. Since the contrast of microscope specimens is ordinarily low, setting the condenser aperture iris diaphragm to 7060% of the N.A. of the objective in use is usually recommended. If necessary, adjust the ratio by removing the eyepiece and looking into the eyepiece sleeve while adjusting the aperture iris diaphragm ring @ until the image shown in Fig. 35 is Seen.
1 Usina the Numerital
Aoet-ture Scale
Set the condenser numerical aperture scale @ to about 80% of the NA value @ of the respective objective. (Fig. 36) Example: With the Plan 40X (NA 0.651, set the scale to 0.65 x 0.8 = 0.5.
Fig. 36
Compatibility
IO-60X 1oox
of Objectives
and Condensers
Top lens in light path
Applicable
Applicable
Condenser NAslightly lessthanperfect**
*l When using the U-SC Swing-out condenser together with a 2X or 4X objective, fully open the condenser aperture iris diaphragm and use the field iris diaphragm in the base as aperture diaphram. ** The slightly lower NA results in a somewhat darker field of view with a IOOX objective, but the combination is acceptable. l To obtain better illumination, use of the U-ULC is recommended in photomicrography when using the 2X or 4X objective.
17
0
0 Adjust the coarse adjustment knob tension using the tension adjustment ring. The coarse adjustment knob tension is preadjusted for easy use. However, if desired, you tan Change the tension using the tension adjustment ring 0. Turning the ring in the direction of the arrow increases tension, and vice versa. The tension is too low if the Stage drops by itself or focus is quickly lost after adjustment with the fine adjustment knob. In this case, turn the ring in the direction of the arrow to increase tension. Fig. 37
0 Ordinarily, the fine adjustment knob is used with the rubber cap attached. However, if space between the knob and the Stage controls is insufficient, the cap may be removed. The cap makes it easier to turn the fine adjustment knob in small increments to obtain more precise focusing.
m 8 5 ? cn
Fig. 38
0 The prefocusing lever ensures that the objective does not come in contact with the specimen and simplifies focusing. After focusing on the specimen with the coarse adjustment knob, turn this lever 0 in the direction of the arrow to set an upper limit on coarse adjustment movement. After changing specimens, refocusing is easily accomplished by rotating the coarse adjustment knob to resch the pre-focused Position, then making fine adjustments with the fine adjustment knob. Fig. 39
0
0 Stage movement with the fine adjustment knob is not affected by this pre-focusing lever.
18
Fig. 40
v This objective is equippsd with an adjustable collar to correct for spherical aberrations caused by too thick or too thin cover glasses. If the thickness of the cover glass is unknown, the Optimum Position for the correction collar tan be obtained by judging the image resolution. When a satisfactory image is not obtained after focusing, rotate the correction collar to the left or the right and refocus to compare the image at both sides. Return the collar to the Position yielding the improved image; then starting from this Position, further rotate the collar slightly to the right or left, refocusing each time, to compare the image at each Position. Repeat this procedure until the Position with Optimum image sharpness is found. Neglecting this adjustment will impede Optimum performante. When rotating the revolving nosepiece, do not tauch the correction collar tindicated by the arrowj.
1. Focus on the specimen with a low power objective. 2. Place a drop of immersion oil (provided) onto the specimen at the area to be observed. 3. Turn the revolving nosepiece to engage the immersion objective, then focus using the fine adjustment knob. + Since air bubbles in the oil will affect the image quality, make Sure that the oil is free of bubbles. a. To check for bubbles, remove the eyepiece and fully open the field and aperture iris diaphragms, then look at the exit pupil of the objective inside the Observation tube. (The pupil should appear round and bright.) b. To remove bubbles, repeatedly defocus and refocus the oil immersion objective. 0 If the condenser engraving Shows a numerical aperture (NA) of 1.O or higher, the number applies only when oil is applied between the slide glass and the top surface of the condenser. When oil is not present, the NA is about 0.9. 4. After use, remove oil from the objective front lens by wiping with gauze slightly moistened with an ether (7 Parts) / alcohol (3 Parts) mixture. A
Caution on Using the Immersion Oil: If the immersion oil Comes into contact with your eye or skin, immediately take the following action. For eye: Flinse with clean water (for more than 15 min.). For skin: Wash with soap and water. If the appearance of the eye or skin changes or pain continues, please consult your doctor.
0 Use a trinocular Observation tube (U-TRSO, U-lTR, U-SWTR) for photomicrography. Photomicrography tan be performed using either the PM-l 0, the PM-20, or the PM-30 photomicrographic System. Procedures for operating the photomicrographic units are described in their respective instruction manuals. Procedures specific to this microscope are described below.
1. Using the Allen screwdriver, loosen the clamping screw @ on the trinocular tube Photo part. 2. Align the index dot @ with the index dot @ on the straight Photo tube, then mount the straight Photo tube on the trinocular tube Photo part. 3. Tighten the clamping screw 0.
Fig. 41
19
0
Use only a PE Photo eyepiece for photomicrography. Insert the Photo eyepiece @ with the desired magnification into the straight Photo tube mounted on the trinocular Observation tube.
Fig. 42
(Fig. 43) Place the Camera unit directly over the circular dovetail of the straight Photo tube. Make sure the index dots @ on the straight Photo tube and the Camera unit are aligned, then clamp the unit.
See page 15 of the “Observation
Tube” section.
Fig. 43
Fig. 44
1. Camera focusing is done using the binocular section of the trinocular Observation tube. + Whenever you remove the focusing telescope from the photographic unit, be sure to install the dust cap. 2. Insert a finder eyepiece into the right eyepiece sleeve. 3. The finder eyepiece has a reticle with four masks and a double crossline, and the focus is for all purposes the same for the reticle and the Camera film plane. The masks indicate the areas covered, and the numerals next to the masks correspond to the magnification of the Photo eyepiece. Different finder eyepieces are available for different cameras. Select the type that is appropriate for the Camera being used. 4. Because of the great depth of focus of 1.25X to 4X objectives, use of a focusing magnifier (U-FT) is recommended for accurate focusing. 0 When using low power objectives, focusing may be accomplished easier when using the focusing telescope on the Camera unit rather than the finder eyepiece.
5 2 f.2
Item 1. Optical System
UIS (Universal
Infinity
System)
Optical System
2. Illumination
Built-in transmitted Koehler illumination (Field number 26.5; super-widefield 6V 30W Halogen bulb (pre-centered) 6V 30WHAL (PHILIPS 5761) Average life time: Approximately 100 hrs. when used as directed) Light intensity voltage range: DC 1.5V-5.9V (continuous) Light intensity preset button (voltage adjustment range: l.SV-5.9V) Rated voltage IOO-12OV/240-220V \ 0.8/0.4A 50/60 Hz
3. Focusing
Stage movement by roller guide (Rack & Pinion) Stroke per rotation: 0.1 mm (fine), 15 mm (coarse) Full stroke range: 25 mm Upper limit stopper Torque adjustment on coarse focus knob
4. Revolving noseoiece 5. Observation tube
Fixed reversed U-BI30
Type
quinfuple
nosepiece
U-TBI-2
Widefield binocular
U-TR30
Widefield tilting binocular
Field No.
applicable)
Widefield trinocular
U-SwrR Super widefield trinocular tube
tube
22
Tube inclination
26.5
5”-35” (continuous)
30”
30”
$,af,ueilh 50mm-76mm
adjustment Light path selector 6. Stage
3 Steps: None U-SVRS (B)
Type
1
U-SVRD (B)
1
U-SVLS (B)
Common axis with low positioned coaxial knobs on the right side (Rectangular ceramic coated Stage) Size Movement mechanism ;Eg;yen 7. Condenser
Adjustable Movement
8. Operating environment
21
Common axis with low positioned coaxial knobs on the left side (Rectangular ceramic coated Stage)
vertical (Y) and horizontal (X) knob tension range: 52 mm Y-direction, 76 mm X-direction
Single slide holder
Double
Single slide holder
slide holder
U-AC
u-sc
Double
slide holder
U-MC
Abbe condenser
Swing-out condenser
Achromat aplanat condenser
1.25
0.9-0.16
1.40
Aperture iris diaphragm Objective range
U-SVLD (B)
135 mm (D) x 180 mm (W)
/
Twe
N.A.
@ BI 100% @ BI 20%, Photo 80% @ Photo, TV 100%
With 4X-1 OOX (for widefield observations) 1OX-1 OOX (for super widefield observations)
numerical
aperture
scale
2X-1 OOX (wide-field/super widefield observations)
1 OX-1 OOX (widefield super widefield observations)
Indoor use Altitude: max. 2000 m . Ambient temperature: 5” to 40°C (41” to 104°F) l Maximum relative humidity 80% for temperatures up to 31°C (88°F) decreasing linearly through 70% at 34°C (93”F), 60% at 37°C (99”F), to 50% relative humidity at 40°C (104°F) l Main supply voltage fluctuations not to exceed *IO% of the nominal voltage l Pollution Degree 2 (In accordance with IEC664) l Installation/Overvoltage Category II (In accordance with IEC664) l l
0
BX40
Magnification Mechanical-----\ tube length w
~. _n ", : -" +---
N.A.
'" : jj -""j : &$
W.P.
1
=,~ “n>~ .,jiL “” ‘n
"n.
Dbpttl‘-
.+y:
:
6.1
1.34
I
0.40 0.65 0.80 1.25 0.6-1.25
3.0 0.45 0.15 0.13 0.13
0.84 0.52 0.42 0.27 0.27
2oox 4oox 600X 1ooox 1ooox
6.09 3.04 1.76 0.69 0.69
IO
0.10 0.25 0.40 0.65 .50-0.9c
22.0 10.5 1.2 0.56 0.20 0.15
3.36 1.34 0.84 0.52 0.37 0.27
4ox 1oox 2oox 4oox 5oox 1ooox
175 28.0 6.09 3.04 1.75 0.69
2.58 1.12 0.67 0.45 0.26 0.26
4ox 1oox 2oox 4oox 1ooox 1ooox
2.1 0.84 0.48 0.39 0.34 0.25
T-
0.25
1.25
l
I I
,
0
0.17 0.17
IOOX
“”
j&+i;
&g; f!yd:.
Cover glass thickness
Field of Vi&4
: :
28.0
150x
20.9
1.4
3oox 600X 9oox l5OOX 15oox
4.64 2.35 1.39 0.55 0.55
0.7 0.35 0.23 0.14 0.14
5.5 2.2 1.1 0.55 0.44 0.22
60X 150x 3oox 600X 750x 15oox
85.8 20.9 4.64 2.35 1.30 0.55
3.5 1.4 0.7 0.35 0.28 0.14
127 22.4 7.00 2.52 0.66 0.66
5.5 2.2 1.1 0.55 0.22 0.22
60X 150x 3oox 600X 15oox 1500x
92.9 16.5 5.22 1.93 0.52 0.52
3.5 1.4 0.7 0.35 0.14 0.14
Iris
4ox 1oox 2oox 4oox 4oox 1ooox
99.5 15.9 4.65 2.14 1.70 0.62
5.5 2.2 1 .l 0.55 0.55 0.22
60X 150x 3oox 600X 600X 1500x
71.7 11.5 3.39 1.62 1.30 0.49
3.5 1.4 0.7 0.35 0.35 0.14
Collar Iris Iris
8.38 4.19 0.35 0.24 0.24
12.5X 2ox 4oox 600X 1ooox
872 398 1.86 0.85 0.59
17.6 11.0 0.55 0.37 0.22
18.75) 3ox 600X 9oox 1500x
639 287 1.40 0.64 0.47
11.2 7.0 0.35 0.23 0.14
0.13 0.30 0.50 0.75 1.30 0.60.1.3[
17.0 10.0 1.6 0.51 0.10 0.10
0.16 0.40 0.70 0.85 0.5-1.00 05-1.35
13.0 3.1 0.65 0.2 0.12 0.10
0.04 0.08 0.95 1.40 1.40
5.1 6.0 0.14 0.10 0.10
0.75
0.6:
0
0.45
4oox
1.66
0.55
600X
1.27
0.35
1.40
O.l(
0
0.24
1 ooox
0.59
0.22
1500x
0.47
0.14
0.17 0.17 0.17 0.17
0.17 0.17 1.11-o 0.17 1.11-o 0.17 0.17
Iris
Iris
Collar
22
Under certain conditions, Performance of this unit may be adversely affected by factors other than defects. If Problems occur, please review the following list and take remedial action as needed. If you cannot solve the Problem after checking the entire list, please contact your local Olympus representative for assistance.
1. Optical System a) Bulb does not light.
Bulb bumed out.
Replace bulb.
3) Bulb operates, but field of view remains dark.
Field iris diaphragm is not opened wide enough.
Open the field iris diaphragm.
16
Condenser is lowered too much.
Adjust the condenser height Position.
16
Light path selector knob is set to in the ~EJposition.
Move the knob to theaffiorafposition.
Light path selector knob is in the middle position.
Set the knob according to the observation method.
The revolving nosepiece is not correctly engaged.
Make sure that the revolving nosepiece clicks proper& into place.
8
An objective that falls outside of the condenser’s illumination range is used.
Use a condenser to match the objective.
17
The condenser is not properly centered.
Center the condenser.
16
The field iris diaphragm is stopped down too far.
Open the field iris diaphragm.
The halogen correctly.
Push the pins of the halogen bulb into the holes.
c) Field of view is obscured, or field of view is not evenly illuminated.
d) Dirt or dust is visible in the field of view.
bulb is not mounted
Dirt on the base light exit glass.
16
Clean thoroughly.
Dirt on the top surface of the condenser. Dir-t/dust on the specimen Dirt/dust on the eyepiece e) The image Shows diffraction.
f) Visibility is poor. l Image is not sharp. l Contrast is poor. l Details are indistinct.
23
16
The condenser is lowered too far.
Adjust the condenser height Position.
The aperture iris diaphragm is stopped down too far.
Open the aperture iris diaphragm.
A non-UIS series objective is used.
Use only UIS series objectives with this microscope.
-
The objective is not correctly engaged in the light path.
Make sure that the revolving nosepiece clicks into place correctly.
8
The correction collar on the correction collar equipped objective is not properly adjusted.
While focusing, turn the correction collar to find the best Position.
19
Front lens of the objective is dirty.
Clean the objective.
19
Immersion oil is not being used with an oil immersion objective.
Use immersion Oil.
19
The immersion oil contains bubbles.
Remove the bubbles.
19
17
0
BX40
f) Visibility is poor. l Image is not sharp. l Contrast is poor. l Details are indistinct.
Recommended immersion oil not used.
Use the provided immersion Oil.
Specimen is dirty.
Clean.
-
Condenser is dirty. Inappropriate Object slide or cover glass thickness.
Replace with glass of recommendec thickness.
12
The objective is not correctly engaged in the light path.
Make sure that the revolving nosepiect clicks into place correctly.
8
The specimen is not correctly mounted on the Stage.
Place the specimen correctly on top o the Stage and secure it with the slidc holder.
The objective is not correctly engaged in the light path.
Make sure that the revolving nosepict clicks into place correctly.
8
The condenser is not properly centered.
Center the condenser.
16
The condenser is not properly centered.
Center the condenser.
16
The condenser is lowered too far.
Adjust the condenser height Position.
16
The bulb is nearly burned out.
Replace the bulb.
A connector is not properly connected.
Check all connections.
b) The bulb burns out almost immediately.
Wrong type of bulb used.
Use the correct bulb type
c) Brightness does not Change when you move the light intensity adjustment lever.
The light intensity preset button is set to ON.
Press the button to OFF
d) The voltage indicator LEDs do not light, or the bulb does not light.
The voltage selector switch is set to the wrong Position.
Set the switch to the Position matchin! to the local line voltage (100 - 120V o 220-24OV).
e) The voltage indicator LEDs all light, and are not affected by the light intensity adjustment lever.
The bulb is not installed.
Install the bulb
The bulb is burned out.
Replace the bulb.
The lamp socket is not connected.
Connect the lamp socket correctly.
g) Part of the image blurred.
is
h) The image appears to waver.
i) The field of view beComes only slightly brighter when the voltage is raised.
a) The bulb intermittently lights and goes out.
b) The Stage drifts down by itself, or focus is lost during Observation.
0
The tension adjustment tightened excessively.
ring
is
>“_ ~‘n”“~;<” .“_ ,: : : “,in “” <“_”
Unlack the pre-focusing
The tension loose.
Tighten the ring.
ring is too
,<
I T 3
18
Loose the ring.
You are trying to raise the Stage with the coarse adjustment knob with the pre-focusing lever engaged. adjustment
3
10
~,: ~”
a) The coarse adjustment knob is hard to turn.
12
lever.
18
r
18
24
c) The image is not focused.
When adjusting the Stage height, you forgot to reattach the upper stopper screw.
Reattach the upper stopper screw.
d) Coarse adjustment will not go all the way up.
You are trying to raise the Stage with the coarse adjustment knob with the pre-focusing lever engaged.
Unlack the pre-focusing lever.
e) Coarse adjustment will not go all the way down,
The condenser holder is too low.
Raise the condenser holder
f) The objective makes contact with the specimen before focus is obtained.
The specimen is mounted upsidedown
Mount the specimen correctly.
4. Observation
Tube
a) Field of view of one eye does not match that of the other.
_’
;
The interpupillary distance is incorrect.
Adjust the interpupillary distance.
14
Incorrect diopter adjustment
Adjust the diopter.
14
Different eyepieces are used on the left and right.
Change one eyepiece to match the other so that both sides are the same type.
The Optical axes are not parallel.
Upon looking into the eyepieces, try looking at the Overall field before concentrating on the specimen range. You may also find it helpful to look up and into the distance for a moment before looking back into the microscope.
><
5. Stage
18
“,n
:
~>
a) The image shifts when you tauch the Stage.
The Stage is not properly mounted.
Clamp the Stage.
b) Specimen Stops midway on the X axis traverse.
eR-~especimen is not correctly position-
Place the specimen correctly.
c) The X axis and Y axis knobs are too tight, or too loose.
Is X or Y axis tension too high or too low?
Adjust the tension.
3
12
12
This device camplies with the requirements of both directive 89/336/EEC concerning electromagnetic compatibility and directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with the above directives. I
25
I
l
BX40
n PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment “Specifications” and “Certified Cord” below: CAUTION:
In case you use a non-approved power supply cord for Olympus Warrant the electrical safety of the equipment.
products,
Olympus
by referring to tan no longer
Specifications Voltage Rating Current Rating Temperature Rating Length Fittings Configuration
125V AC (for I OO-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum 3.05 m maximum Grounding type attachment plug cap. Opposite terminates in molded-on IEC configuration appliance coupling.
Table 1 Certified
Cord
A power supply cord should be certified by one of the agencies listed in Table 1, or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In case you are unable to buy locally in your country the power supply cord which is approved byone of theagencies mentioned in Table 1, please use replacementsapproved byanyother equivalent and authorized agencies in your country
Ireland
0
NSAI
26
Table 2 HAR Flexible APPROVAL ORGANIZATIONS
Comite Electrotechnique (CEBEC)
AND CORDAGE HARMONIZATION
Belge
Verband Deutscher Elektrotechniker (VDE) e.V. Prüfstelle Union Technique (UTE)
de d’Electricite’ /
Institut0 Italiano del Marchio di Qualita’ (IMQ) British Approvals Cables (BASEC)
/
Service for Electric
N.V. KEMA
/
SEMKO AB Svenska Elektriska Materielkontrollanstalter
I
Österreichischer Verband für Elektrotechnik (ÖVK)
/
Danmarks (DEMKO)
Elektriske
National Standards Ireland (NSAI) Norges Elektriske (NEMKO) Asociacion Electronica
Materielkontrol Authority
Institut0 (IPQ)
Electrotecnica Y Espanola (AEE)
Portugues
Schweizerischer Verein (SEV) Elektriska Underwriters
for Standardiza-
da Qualidade
Elektro Technischer
Inspektoratet Laboratories
Canadian Standards
27
of
Materiellkontroll
Hellenic Organization tion (ELOT)
I Inc. (UL)
Association
(CSA)
Cord MARKING METHODS
CEVEC
10
30
USE
1
30
1
30
/
IO
IEMMEQU
/
10
1
30
1
50
BASEC
KEMA-KEUR
1
10
/
30
!
30
SEMKO
1
10
/
10
/
50
<öVE>
(
30
1
10
1
50
/
30
/
10
1
30
30
30
50
NEMKO
10
10
70
30
10
70
ELOT
30
30
70
I np I
SEV
10
30
90
SETI
10
30
90
I
1
30
/
10
10 10
SV, SVT, SJ or SJT, 3 X 18AWG SV, SVT, SJ or SJT, 3 X 18AWG
l
OWMPUS
OPTICAL
Z-43-2,Hatagaya,
Shibuya-ku,
CO.,LTD.
OWMPUS
OPTICALCO.
OLYMP&
AMERICA
OLYMPUS
SINGAPORE
OLWPUS
OPTICAL
OWMPUS
AUSTRALIA
Tokyo, Japan
(EUROPA)
Postfach 10 49 08, 20034, Hamburg, Germany
INC.
2 Corporate Center Drive, Melville,
NY 11747-3157,
GMBH.
U.S.A.
PTE LTD.
491 B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore
248373
CO. (UKJ LTD.
2-8 Honduras Street, London ECIY OTX, United Kingdom.
PTY. LTD.
104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia
This publication Printed
is printed on recycled Paper.
in Japan
2000 06
M 050-0
