Transcript
Sabancı University Nanotechnology Research and Application Center (SUNUM)
LABORATORY SAFETY SEMINAR
Introduction
Part 1. SUNUM Facilities
Part 2. Emergency Procedures Part 3. Hazards & Safety
Architecture
Architecture inspired by human cell
Architecture
BASEMENT
Half basement added later
7.400 m2 research building 2 full floors and half basement
Layout: Lab floor
Layout: Lab floor
Vibration isolation
Layout: Lab floor Dedicated Laboratories • Micro/Nano Fabrication Lab (Class 100- 1000 clean room) • Molecular Biology (Genome sequencing capability) (BSL2) • Electron Microscopy and Spectroscopy • Material Growth • Material Characterization • Nanoelectronics • Nanomagnetics • Energy Systems • Micro/Nano-fluidics • Micro-Nano Packaging • Advanced Microscopy • Testing & Characterization (up tp 60 GHz)(Anechoic chamber) • 3D Micro/Nano Prototyping • Tissue and Regenerative Engineering (BSL2) • Precision Manufacturing
Green Building
Conforming to international ‘Green’ standards: LEED - Gold Level (USA) and BREEAM (English) certified
Built on a parking lot to be ecologically biocompatible with the surroundings
Endemic, low water consuming plants selected for landscape: 72 trees and bushes, 5749 plants, 7380 ground-covers replacing and adding to 795 transplanted species
Recovery and reuse of rainwater
Large area thermally isolating windows and glass roof for natural lighting
Minimal water usage in the building by atomated turn-off, low debit fixtures
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Supplied Services ASTM Type 1 and Type 2 DI water (8 and 18 Mohm loops) 5 different gases centrally supplied using high purity lines Aircuity environment control for hazardous conditions
Common neutralization system for laboratory water, acid and alkaline discharge
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Gases, Vacuum and Compressed Air 5 different gases distributed to all labs from an enclosed location placed outside of the buiding under one of the stairs One wall of the enclosure weakened to absorb the schock of explosions Services provided: Ar, High Purity N2, H2, Spare, Liquid nitrogen Tank (3T) outside of the building for common N2 Vacuum and dry-generated compressed air services in the basement
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LAB SAFETY • • • • • • •
General Principles Emergency Procedure Chemical Hygine Plan Storage Chemical Spill Hazardous Waste Disposal First Aid
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General Safety Guidlines • • • • • • • • • • • • • • • • • • • • •
NEVER perform any hazardous work alone in the laboratory. At least two people should be present. NEVER perform unauthorized work, preparations or experiments. ALWAYS wear proper eye protection in chemical work, handling and storage areas. Contact lenses should normally not be worn. Prepare and maintain a chemical inventory for the lab. Consult material safety data sheets (MSDS) before working with hazardous chemicals or infectious material. ALWAYS know the hazards associated with the materials that are being utilized in the lab. ALWAYS wear appropriate protective clothing. Cover shorts, cut-offs or miniskirts with a suitable lab coat or apron. Confine long hair and loose clothing. Do not wear high-heeled shoes, open-toed shoes, sandals or shoes made of woven material. ALWAYS wash hands and arms with soap and water before leaving the work area. NEVER remove chemicals, biological agents, or radioactive materials from the facility without proper authorization. Be familiar with the location of emergency equipment - fire alarm, fire extinguisher, eye wash stations and safety showers. Know the appropriate emergency response procedures Use equipment and hazardous materials only for their intended purposes. NEVER mouth pipette chemicals when transferring solutions. Instead, you should always use a pipette bulb to transfer solutions. Use a hood whenever there is a possibility of poisonous or irritating fumes being emitted. NEVER leave an experiment unattended while it is being heated or is rapidly reacting. Keep equipment back from the edge of the lab bench to prevent spillage. Support all beakers and flasks with clamps. Do not use cracked or chipped glassware. NEVER engage in horseplay, pranks or other acts of mischief in chemical or biological work areas Report accidents and dangerous incidents ("near-misses") promptly to your supervisor and SUNUM Facilities Management. Inform your supervisor and SUNUM Facilities Management if you realize that any equipment is broken, not working properly or not calibrated.
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Emergency Procedures
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Emergency Procedures
Question: What should
you do in an emergency? • Fire? • Accidents? • Other emergencies?
Answer:
Call 5555 or 6666 from any campus phone
Emergency Procedures Fire Emergency 1. NOTIFY people in the immediate area that there is a fire 2. ACTIVATE the nearest FIRE ALARM 3. Locate the nearest campus telephone away from the fire area and DIAL 5555 4. FIGHT THE FIRE IF YOU CAN (see side panel) 5. EVACUATE the area by using the stairwells. 6. NEVER re-enter the building until told by Officials
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Emergency Procedures Earthquake Response
1. “DROP, COVER AND HOLD ON.” 2. AVOID windows, hanging objects, mirrors, tall furniture 3. DO NOT TRY TO RUN OUT OF BUILDING. 4. Do not use elevators. 5. EVACUATE the area by using the stairwells. 6. NEVER re-enter the lab until told by Officials.
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Emergency Procedures Hazardous Material Spill/Gas Leak 1. ALERT people in the laboratory to evacuate. 2.ACTIVATE the Emergency Button next to lab door. 3.CALL SUNUM Facility Management 2424 or 5555 any time. 4. ISOLATE the affected area. 5.NEVER re-enter the lab until told by Officials
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Emergency Evacuation Plan
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LAB EMERGENCY POSTER Call 5555 Medical Emergency Dial 5555 or 6666
LIFE THREATENING EMERGENCY:Dial 5555 or contact the University Medical Center Emergency directly at 6666 (located at D2 building-2 Floor). Note: All injuries must be reported to SUNUM Facilities Management within 8 hours. NON-LIFE THREATENING EMERGENCY: Go to University Medical Center. Needle stick/puncture exposure: Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Note: All needle stick/puncture exposures must be reported to SUNUM Facilities Management within 8 hours.
Fire Dial 5555
SMALL FIRE (trash can size): If you have been trained, you may put out the fire using a fire extinguisher. Report the fire by calling 5555. Notify Security 2440 and SUNUM Facilities Management at 2424 at anytime a fire extinguisher is used or discharged. LARGE FIRE (requiring more than 1 fire extinguisher): Evacuate the area. Close all doors and windows as you leave. Close the fume hood sash if the fire is in the fume hood. Activate the nearest alarm. Call 5555. Evacuate the area using the stairwells. Do not use the elevator. CLOTHES ON FIRE: Use nearest safety shower. If none immediately available, STOP-DROP-ROLL to quickly smother the fire. Seek medical attention. Notify Security and Health Center at 5555 immediately.
Chemical Spill Dial 2424
SPILL: Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.). SMALL (<1 L) :If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up. LARGE (>1 L): Evacuate the lab, Push Lab Emergency button and Dial SUNUM Facilities Management 2424 and for assistance. CHEMICAL SPILL ON BODY OR CLOTHES: Remove clothing and rinse body thoroughly in emergency shower for at east 15 minutes. Seek medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management 2424 immediately. CHEMICAL SPLASH INTO EYES: Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424 immediately.
Bio-hazardous Spill Dial 2424
CONCENTRATED, 100 ml OF BSL- 2 OR SPILL IN PUBLIC AREA – Do not attempt to clean it up. Keep people from entering. Dial SUNUM Facilities Management 2424 for assistance. BIOHAZARDOUS SPILL ON BODY OR CLOTHES: Immediately remove contaminated clothing and place in a red biohazard bag. Wash with antiseptic soap and water for at least 15 minutes. Seek medical attention. Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424 immediately. GENERAL BIOHAZARD SPILL CLEAN-UP: Use clean personal protective equipment appropriate for the lab Biosafety Level. Place absorbent pads over area. Use appropriate disinfectant. Allow a minimum 20 minutes of contact time. Remove any sharps and discard in a sharps container. Dispose clean-up materials as biohazardous waste for proper waste disposal. Remove protective clothing and segregate for disposal.Wash hands with soap and water. Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424 immediately INERT GAS LEAKAGE: Move the cylinder to an isolated, well-ventilated area. Notify SUNUM Facilities Management 2424 immediately. If large or uncontrollable leak occurs, evacuate the area and Notify SUNUM Facilities Management 2424 immediately. UNKNOWN, TOXIC,FLAMMABLE GAS LEAKAGE: Evacuate the lab, Push Lab Emergency button and Dial SUNUM Facilities Management 2424 and for assistance INHALED: Remove to fresh air and Call 6666 for immediate medical attention. CONTAMINATION: Remove clothing and rinse body thoroughly in emergency shower for at east 15 minutes. Seek medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management 2424 immediately. EYE CONTACT: Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical attention, Dial Security and Health Center at 5555 immediately. Notify SUNUM Facilities Management at 2424.
Gas-Leakage Dial 2424
Earthquake
Stay calm. Remember to DUCK, COVER, AND HOLD. If you are INDOORS, stay there. Do not run outside. Take cover under a heavy desk or table or in a doorway and protect your neck and head. If you are outdoors Move to a clear area if you can safely walk. Avoid buildings and trees. After an earthquake, use caution, carefully evacuate your residence and go to the Emergency Assembly Point – FASS PARKING LOT. Make sure you are accounted for before heading elsewhere.
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Injury Statistics Laboratory support injuries, 1993 - 1997* The most common serious injuries: strains and fractures due to falls and improper lifting of heavy objects.
Source: Howard Hughes Medical Institute, Office of Laboratory Safety. All reported injuries are shown, without regard to severity. Source: U.S. Department of Labor, Bureau of Labor Statistics(BLS)'s 2005
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Accident/Incident/Hazard Form
Should be filled by EVERYONE Complete form within 24 hours of injury
Submit completed report to your supervisor/FENS/SUNUM Form is submitted to S.U Health&Safety Department
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Hazards and Safety
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Chemical Hygiene Plan Chapter 4.Chemical Hygiene Plan 4.1.1. Hazardous Chemical Definition 4.1.2. Hazard Identification 4.2. Training&Information 4.3. Standart Operation Prodedures 4.4. Protective Equipment
4.5. Laboratory Safety Equipments 4.6. Chemical Procurement, Distribution Storage
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Chemical Hygiene Plan, Ch. 4.1.1 Hazardous Chemical Definition • Carcinogens • Reproductive toxins • Sensitizers • Hepatotoxins • Agents that act on the hematopoietic system
• Irritants • Corrosives • Neurotoxins • Nephrotoxins • Asphixiants
• Agents that damage the lungs, skin, eyes, or mucus membranes
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Chemical Hygiene Plan, Ch. 4.1.2 Hazard Identification, Risk Analysis
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Chemical Hygiene Plan, Ch. 4.2 The content of the training programs will include the following: • • • • • •
University chemical hygiene policies; and Applicable details of the University's Chemical Hygiene Plan. Appropriate response in the event of a chemical emergency (spill, overexposure, etc.); Physical and health hazards of various classes of laboratory chemicals; Methods/procedures for handling and safely using chemicals present in laboratories; Additional laboratory specific safety training is provided by the supervisor.
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Chemical Hygine Plan, Ch.4.2. Name of Chemical
Chemical Formula or IUPAC NAME
CAS registry number
20x TE Buffer 2-Propanol 3,3′,5,5′-Tetramethylbenzidine 3,3′-Dithiodipropionic acid Acetone Acetone Acetone
n/a isopropyl alcohol C16H19N 3,3′-Dithiodipropionic acid Acetone 2-propanone (CH3)2CO
not required 67-63-0
Acridine
C13H9N
Acrylamide Agencourt AMPure XP Ammonia Solution %25 Ammonia solution 32% Ammonium acetate Ammonium dihydrogen phosphate Ammonium persulfate Ammonium Persulfate Ammonium sulfate Aniline APS Baytubes C 150 P Baytubes C 70 P BCA Protein Assay Reagent (bicinchoninic acid) Boric acid Buffer EB (MinElute kit component) Buffer PB (MinElute kit component) Buffer PE (MinElute kit component) Buffer pH=7.0,pH=10.01, pH=4.01 Buffered oxide etch Cadmium chloride Calcium chloride Calcium nitrate tetrahydrate Carboxymethyl Cellulose, Sodium Salt Chitosan-LMW Chloroform Chloroform Chloroform
Manufa Catalog cturer # Invitrogen Sigma sigma Sigma Uzsol-A Merck
P7589_B I9516-500ML 860336-1G 109010-50G
260-94-6
Sigma
A23609-5G
C3H5NO n/a NH3 NH3 C2H7NO2 (NH4)H2PO4 Diazanium sulfate Ammonium Persulfate (NH4)2SO4 Aniline (NH4)2S2O8 Multi-walled carbon nanotube Multi-walled carbon nanotube C20H10N2O4 Trihydroxidoboron n/a Guanidinium chloride (25-50%), propan-2-ol (25-50%) n/a Buffer pH=7.0,pH=10.01, pH=4.01 BOE or BHF CdCl2 Calcium chloride Ca(NO3)24H2O Carboxymethyl Cellulose, Sodium Salt Chitosan-LMW CHCl3 CHCl3 CHCl3
79-06-1 none 1336-21-6 631-61-8 7722-76-1 7783-20-2 7783-20-2 7783-20-2 62-53-3 7727-54-0 7440-44-0 7440-44-0 979-88-4 10043-35-3 not required 50-01-1; not required
Sigma Beckman Coulter MERCK Merck Sigma Carlo Erba Sigma Sigma Aldrich Sigma Sigma Aldrich Merck Bayer MaterialScience Bayer MaterialScience Sigma Sigma Qiagen Qiagen Qiagen
A3553-500G A63881 1.054.322.500 1.05426.2500 A1542-500G 419787 A3678-25G
N/A 10108-64-2 10043-52-4 13477-34-4 9004-32-4 9012-76-4 67-66-3 67-66-3 67-66-3
Technic France Sigma Sigma Merck Sigma Aldrich Sigma Aldrich Merck Sigma Merck
Citric acid
C6H8O7
77-92-9
sigma
C2404-100G
D-(+)-Trehalose dihydrate
C12H22O11
6138-23-4
Sigma
T9531-25G
Dimethyl sulfoxide
C2H6OS
67-68-5
Sigma
D2650-100ML
DL-Dithiothreitol DNA 7500 kit DNA standard (100ug/ml in TE buffer) Ethanol Ethanol Ethidium bromide solution Ethylene glycol Ethylenediaminetetraacetic acid disodium salt dihydrate GelPilot 5x loading dye (MinElute Kit component) Glutaraldehyde solution
C4H10O2S2 n/a n/a Ethanol Ethanol 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide C2H6O2 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo)tetraacetic acid Trometamol (1.0-2.5%) C5H8O2
27565-41-9 not required not required 64-17-5 64-17-5 1239-45-8 107-21-1 60-00-4 77-86-1 111-30-8
Sigma Agilent Invitrogen Fisher Scientific Uzsol-E Sigma sigma Sigma Qiagen Sigma
43817-5G 5067-1506 P7589_C BP2818-4
Glycine GS FLX Titanium Control Bead Kit GS FLX Titanium General Library Preparation Kit GS FLX Titanium LV/MV/SV emPCR kit (Lib-A) GS FLX Titanium LV/MV/SV emPCR kit (Lib-L) GS FLX Titanium Maintenance Wash Kit GS FLX Titanium Paired End Adaptor Set GS FLX Titanium PicoTiterPlate Kit 70x75 GS FLX Titanium Rapid Library MID Adaptors Kit GS FLX Titanium Rapid Library Preparation Kit GS FLX Titanium Sequencing Kit XLR70 HEPES buffered saline
C3H7NO5P n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
56-40-6 not required not required not required not required not required not required not required not required not required not required 7365-45-9
Sigma Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Roche 454 Sequencing Sigma
G8898-1KG 05 470 129 001 05 233 747 001 05 619 (114/149/165) 001 05 618 (428/436/444) 001 04 932 358 001 05 463 343 001 05 233 682 001 05 619 211 001 05 608 228 001 05 233 526 001 51558-50ML
54827-17-7 1119-62-6 67-64-1 67-64-1 67-64-1
Package
Chemical Inventory
Plastic Bottle
Plastic Bottle Plastic Bottle
Plastic Bottle Glass Bottle Glass bottle Plastic Bottle
A4418-500G 1012010500
23225 B6768-1KG 28004/28006 28004/28006 28004/28006
All chemicals in the Center (liquids, gases and other materials) is controlled
The list of allowed chemicals is published
Glass bottle
Only chemicals in the list is allowed in the Center
Card box containing multiple vials Plastic Vial Amber glass bottle
Contact SUNUM management to add your chemicals to the list
SUNUM maintains a full set of MSDS for materials in the Center
Plastic bottle Plastic Bottle Plastic Bottle
Plastic bottle Plastic bottle Plastic bottle Plastic Bottle
655198-5G C1016-100G 1021210500
Plastic Bottle
Dark Glass Bottle C2432-500ML 1.02445.2500
E1510-10ML 324558-100ML E5134-100G 28004/28006 G5882-50ML
Plastic vial
Card box Card box Card box Card box Card box Card box Card box Card box Card box Card box
containing multiple vials containing multiple vials containing multiple vials containing multiple vials containing multiple bottles containing multiple vials containing multiple vials containing multiple vials containing multiple bottles
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Chemical Hygine Plan, Ch.4.2.3 Labeling
All chemicals (including solutions and chemicals transferred from their original containers) should be labeled with their common names, concentrations and hazards. Additional information such as: date received, date opened, date of expiration should also be recorded.
Label may also include: Proper handling, Storage, Emergency response 28
Chemical Hygine Plan, Ch.4.2.3 MSDS .MSDS
Includes the following: Product Information Fire and Explosion Toxicology Health Effects PPE Storage Leaks and Spills Waste Disposal First Aid US&A (v 2/07)
Chemical Hygine Plan, Ch.4.2.3 MSDS • The purpose of a Material Safety Data Sheet (MSDS) is to provide readily accessible information on chemical substances commonly used in the science laboratory or in industry. • The MSDS should be kept on file and referred to BEFORE handling ANY chemical. The MSDS can also be used to instruct students on chemical hazards, to evaluate spill and disposal procedures, and to warn of incompatibility with other chemicals or mixtures. These also provide you with the First Aid measures for the stated chemical. Materials Safety Data Sheet (MSDS) Websites • http://www.fishersci.com • http://www.vwr.com • http:/www.hazard.com • http://www.sigmaaldrich.com/ • Plus any vendor you order from must be able to provide an MSDS for your product on request.
Hazard Placards What are the health hazards? What are the fire hazards? What are the reactivity hazards? Are there any special hazards?
4 = Severe Hazard 3 = Serious Hazard 2 = Moderate Hazard 1 = Slight Hazard 0 = Minimal Hazard
Hazard Placards Perchloric Acid
Xylene
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Additional Hazard Ratings Hydrochloric Acid
Reagent Alcohol
7452 Accelerator
Acetic acid, glacial
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Protective Clothing and Lab. Safety Equipment • • • •
Laboratory coat, Gloves, Protective eyewear Closed toe shoes
are must to be worn in SUNUM laboratories WHENEVER handling hazardous chemicals. • Additional personal protective equipment, such as face shield, utility gloves, aprons, and respirators, may be necessary depending on an assessment of the hazard and operation.
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Protective Clothing and Lab. Safety Equipment
• Gloves – – – – – – – – –
Cotton Leather Latex Viton Butyl Neoprene PVC Nitrile Other
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Protective Clothing and Lab. Safety Equipment
Gloves • Be sure you know which type of glove is appropriate for the chemical you are using
Example of wrong glove having been used
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Protective Clothing and Lab. Safety Equipment
Gloves
Glove
Intended Use Advantages
Material • Good for biological and
• Poor for organic solvents
Latex exam
water-based materials
• Hard to detect puncture holes
style
• User acceptability
• Latex allergy issues
• Good for solvents, oils,
• Slightly more expensive than
greases, some acids and
latex
Incidental Contact
Incidental Contact
• Check “Chapter 4.4.6.1 Glove Selection Guide’’ • (Xylenes: Nitrile) • (Perchloric Acid: Nitrile, Neoprene or PVC)
Disadvantages
bases
Nitrile exam
• Clear indication of tears
style
and breaks • User acceptability
• Good for solvents, oils,
• Not effective for
Utility style
greases, some acids and
halogenated and aromatic
Nitrile
bases
hydrocarbons
Extended Contact
• Can be washed and
– Solvex Neoprene –
reused Extended contact
• Poor for halogenated and
alcohols, fuels,
aromatic hydrocarbons
peroxides,hydrocarbons,
utility
and phenols
style Butyl rubber
• Good for acids, bases
Extended contact
• Good for ketones and esters
utility gloves
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Protective Clothing and Lab Safety Equipment
Gloves
NEVER leave the lab with gloves Do not touch common external surfaces such as door knobs with gloves
Remove soiled gloves: If gloves become soiled or contaminated during experiment, stop, and remove carefully Wash your hands and put on new pair of gloves. If you double-glove, remove soiled glove, replace with new one, and continue your work.
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Protective Clothing and Lab Safety Equipment W ear Eye P rotection W hen Using: Glassware under reduced pressure Cryogenic materials Glassware under elevated pressure Explosives Caustics, Irritants or Corrosives Biohazards Radioactive Materials UV Light Toxic Chemicals Carcinogens Flammable Materials Lasers
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Protective Clothing and Lab Safety Equipment
Eye Protection
Wear goggles when using acids or whenever there is potential for a splash hazard
Wear safety glasses at other times when laser light or chemicals are present and when handling glassware
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Storage • Separate chemicals into compatible groups •
Designate separate storage for highly toxic chemicals
• Do not store more chemicals than you will need over a reasonable time
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Storage (continued) Store corrosive, toxic, and highly reactive chemicals in a well-ventilated area
Store chemicals that can ignite at room temperature in a flammables cabinet
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Storage (continued)
Never store liquid hazardous chemicals above eye level
Chemicals stored overhead should be kept on shelves with raised edges.
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Storage (continued)
• Don’t store water reactive chemicals under sink • Store heavy chemical containers on lower shelves and not on floor
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Storage (continued) Never store highly reactive chemicals longer than 6 months. Examples: • Picric acid • Ethyl ether (diethyl ether) • Phosphorus • Sodium metal
• Potassium metal
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Examples of Poor Storage
•Never store a chemical with a missing or obscured label
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Leaks and Spills • • • • •
•
• • •
Alert people in immediate area of spill. Increase ventilation in area of spill (open windows, turn on hoods). Wear protective equipment, including safety goggles, gloves, long-sleeve lab coat and closed toe shoes. Avoid breathing vapors from spill. Use appropriate kit to neutralize and absorb inorganic acids and bases. Collect residue, place in container, and dispose as hazardous chemical waste. Call Lab Supervisors for disposal information, if necessary. For other chemicals, use appropriate kit or absorb spill with vermiculite, dry sand, diatomaceous earth, spill pads, or paper towels. Collect residue, place in container, and dispose as chemical waste. Clean spill area with water.
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Chemical Spills -- Minor • Spill considered minor only if person who spilled it is familiar with chemical, knows associated hazards and knows how to clean up spill safely
Chemical Spills -- M inor
Minor Spills Alert coworkers and proceed to clean up spill Contact SUNUM Facilities Management & University Services for disposal of spill clean-up materials
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Chemical Spills -- Major
Major Spills • Anything beyond minor spill • Anything requiring help from outside of the lab group
Chemical Spills -- Major • • • •
• • •
Attend to injured or contaminated persons and remove them from exposure. Push the Emergency Button next to lab door. Alert people in the laboratory to evacuate. If spilled material is flammable, turn off ignition and heat sources. Place spill cleanup material over spill to keep substance from volatilizing. An MSDS contains special spill clean-up information and should also be consulted. Call SUNUM Facility Management 2424 or 5555 any time. Close doors to affected area. Have a person with knowledge of the incident and laboratory available to answer questions from responding emergency personnel.
Minor Spill Response • Be prepared for emergencies • Ask your Lab. Supervisors for Emergency Spill Kits.
Biohazardous Spill Cleanup Procedures
• Sample of spill cleanup plan
Hazardous Waste Disposal Rules • Minimize • Containerize • Label See Hazardous Waste Disposal Rules: Health and Safety Handbook, Chapter 12.
Hazardous Waste Disposal Rules • Minimize • Containerize (Chemical waste in general, and hazardous waste in particular, must be stored in sturdy container, free of leaks.)
• Label
Hazardous Waste Disposal Rules • Minimize • Containerize • Label (All hazardous waste containers must be properly labeled.)
Hazardous W aste Disposal Rules
Do not use chemical symbols or abbreviations when labeling your containers
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Hazardous Waste Disposal Rules
Autoclave Infectious Waste – or Contact SUNUM Facilities Management (2424) for disposal
Sharps Disposal Put sharps in punctureresistant, leak-proof, sharps container. Label the container with the word, "SHARPS”. If not biohazardous, deface any "BIOHAZARD" markings or symbols. Seal container tightly.
•Contact SUNUM Facilities Management for pickup. 59
First Aid -- Small Cuts, Scratches Small cuts and scratches: • Cleanse area with soap and water • Place a clean dressing over the wound • University Health Center is available to evaluate patients for minor injuries which may require sutures, tetanus booster, etc.
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First Aid - Wounds Wounds -- Significant bleeding Call 6666 IMMEDIATELY
Calm and reassure victim Lay victim down. (Reduces chance of fainting) DO NOT remove any objects impaled in victim Put direct pressure on wound with a sterile bandage or clean cloth If direct pressure does not control bleeding, elevate wound above the heart if possible If bleeding is severe elevate victims legs about 12 inches, and cover victim with a blanket DO NOT APPLY TOURNIQUET
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First Aid - Chemical Burns (Eyes) • Forcibly open eyelids to ensure effective washing behind eyelid • Wash from nose out to ear to avoid washing chemicals back into eye or into an unaffected eye
Flood eyes and eyelids with water/eye solution for minimum of 15 minutes
First Aid - Chemical Burns (Eyes) Remove contact lenses immediately to rinse eyes of harmful chemicals Cover eyes with clean or sterile gauze Call 6666 or proceed directly to the Emergency Room
Make sure you know the location of the nearest eyewash station 63
Chemical Burns - Skin Remove any contaminated clothing Chemicals may collect in shoes, remove shoes, if appropriate Rinse area with large quantities of water for at least 15 minutes (sink, shower or hose)
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First Aid - Chemical Burns - Skin DO NOT apply burn ointments/spray to affected areas Cover with dry clean or sterile material For large affected areas, call 6666 If possible, bring the container of the chemical and/or its MSDS along with you to the evaluating medical facility.
REPORT ALL INJURIES, no matter how small (investigations of near misses lead to fewer future injuries) 65
First Degree Thermal Burns In general first degree burns have redness only, no blister or more extensive tissue damage. Run cool water over area of burn or soak in cool water for at least 5 minutes. Use only WATERJEL-Burn Kits DO NOT APPLY ANY OINTMENTS, SPRAYS OR SALVES. Note: Burns can at first appear to be first degree, but blisters may develop over the next several hours. When in doubt, seek medical attention 66
