Lavision

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Product-Manual Mitas Item-Number(s): 1108630 LaVision We count on Photons Product-Manual for DaVis 7.2 LaVision GmbH, Anna-Vandenhoeck-Ring 19, D-37081 Göttingen Produced by LaVision GmbH, Göttingen Printed in Germany Göttingen, October 10, 2008 Document name: 1003026_FlowMaster_Mitas_D72.pdf Contents Contents 1 Safety Precautions 1.1 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Camera / Image Intensifier Safety . . . . . . . . . . . . . . . . . 5 5 7 2 Introduction 9 3 MITAS hardware 3.1 Mitas setup . . . . . . . . . . . . 3.1.1 Cable connection . . . . . 3.1.2 Microscope lens assembly 3.1.3 End stop adjustment . . . 3.1.4 Camera assembly . . . . . 3.1.5 Light source assembly . . 3.2 MITAS controller . . . . . . . . . 3.3 Filter cube . . . . . . . . . . . . 3.4 Target LED . . . . . . . . . . . . . . . . . . . . . 11 11 11 12 13 14 14 14 16 17 4 Operation 4.1 Focusing on micro structures . . . . . . . . . . . . . . . . . . . 19 19 5 Software Settings 5.1 DaVis Hardware Setup . . . 5.1.1 PTU . . . . . . . . . 5.1.2 Timing Setup . . . . 5.1.3 Line Configuration . 5.1.4 PTU Light Source 1 5.1.5 Microscope . . . . . 5.2 Device Settings . . . . . . . 5.2.1 Motor Settings . . . 5.2.2 Reference drive . . . 5.2.3 Increments . . . . . 21 21 21 22 22 23 23 24 25 26 26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Contents 4 1 Safety Precautions Before working with your LaVision system we recommend to read the following safety precautions. Observing these instructions helps to avoid danger, to reduce repair costs and downtimes and to increase the reliability and life of your LaVision system. 1.1 Laser Safety If a laser1 is integrated in your system it is important that every person working with it has fully read and understood these safety precautions and the laser manual of the specific laser. Lasers included in LaVision systems may belong to CLASS 4 laser devices, which are capable of emitting levels of both visible and invisible radiation that can cause damage to the eyes and skin. It is absolutely necessary that protective eyewear with a sufficiently high optical density is worn at any time when operating the laser. The goggles must protect against all wavelengths that can be emitted, including harmonics. See your Laser´s manual for further details. Class 4 laser beams are by definition a safety and fire hazard. The use of controls, adjustments or performance of procedures other than those specified in the LaVision manual and laser manual may result in hazardous radiation exposure. AVOID EYE AND SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION. FOLLOW THE INSTRUCTIONS YOU CAN FIND IN THE CORRESPONDING LASER MANUAL FOR PROPER INSTALLATION AND SAVE OPERATION. USE PROTECTIVE EYE WEAR ALL THE TIME WHEN OPERATING THE LASER. Important instructions for safe laser handling: • Before operating the laser contact your laser safety officer. 1 In the following ‘laser’ means any kind of laser, in particular Nd:YAG- and dye laser as well as Optical Parametric Oscillators at any wave-length and output-energy. 5 1 Safety Precautions • Read and understand the instruction manual of the particular type of laser. Take special care to laser emission, high voltage and hazardous gases if in use. • Declare a controlled access area for laser operation. Limit access to trained people. Never operate the laser in a room where laser light can escape through windows or doors. If possible, cover beam paths to avoid obstacles getting into the beam. • Provide adequate and proper laser safety-goggles to all persons present who may be exposed to laser light. The selection of the goggles depends on the energy and the wavelength of the laser beam as well as the operation conditions. Check the Laser´s manual for a detailed description. • While working with lasers do not wear reflective jewelry like watches and rings, as these might cause accidental hazardous reflections. • Avoid looking at the output beam, even diffuse reflections can be dangerous. • Operate the laser at the lowest beam intensity possible. • Avoid blocking the output beam or reflections with any part of the body. Use beam dumps to avoid reflections from the target. • Wear clothes and gloves which cover arms and hands to avoid skin damage when handling in the optical path. Especially UV-radiation can cause skin cancer. 6 1.2 Camera / Image Intensifier Safety 1.2 Camera / Image Intensifier Safety The camera integrated in your system is based on a CCD (Charge Coupled Device) or CMOS (Complementary Metal-Oxide Semiconductor) sensor with high resolution and high sensitivity. Optionally your system is equipped with a built-in or external image intensifier. A LASER BEAM FOCUSED ON THE CHIP OR INTENSIFIER, EITHER DIRECTLY OR BY REFLECTION, CAN CAUSE PERMANENT DAMAGE TO THE CHIP OR INTENSIFIER. ANY LASER POWERFUL ENOUGH TO PRODUCE LOCALIZED HEATING AT THE SURFACE OF THE CHIP OR INTENSIFIER WILL CAUSE DAMAGE EVEN WHEN THE CAMERA OR INTENSIFIER POWER IS OFF. A CHIP OR INTENSIFIER DAMAGED BY LASER LIGHT IS NOT COVERED BY ITS WARRANTY. Important instructions for safe camera handling: • Fully read and understand the instruction manual of the specific type of camera. • Put the protection cap on the camera lens whenever you do not take images, especially when the laser beam is adjusted. Switching off the camera / image intensifier does not protect the chip from damage by laser light. • Use full resolution of the sensor and always read out the complete chip to have control of the intensity on all areas of the sensor. • Make sure that no parts of the image are saturated, i.e. the intensity is below maximum gray level (< 4095 counts for a 12 bit camera, < 65535 counts for a 16 bit camera, ...). • Start measurements with the lowest laser power and a small aperture of the camera lens. • Increase laser power step by step and check the intensity on the corresponding image. Make sure that the sensor does not run into saturation. • Bright parts in the experiment, like reflections on walls or big particles, will limit the maximum laser power. Modify the optical arrangement of your setup in order to remove bright reflections from the camera image. 7 1 Safety Precautions 8 2 Introduction LaVision’s FlowMaster MITAS is an inverted laser imaging microscope system for the routine and laboratory use in microfuidic fows. MITAS supports different imaging analysis techniques to measure flow fields, concentration, mixing, particle size and motion and fluid temperature with high spatial and temporal resolution. The FlowMaster MITAS laser imaging system comprises a fully motorized 3-axis microscope stage with a high performance controller and a high precision microscope objective. For standard PIV applications a DPSS laser for pulsed or cw illumination and a high sensitive, double-frame CCD camera are used. A system PC with built-in synchronization unit controls the complete laser imaging system. LaVision’s modular DaVis software is used for advanced image acquisition and data analysis and stereoscopic imaging in general. The xyz (focus)-traverse system can be operated manually using a joy stick or the device control manager in DaVis. The laser light is delivered through an optical fiber to the microscope. A built-in pilot LED is used for target focusing. The filter cube exchange box allows a fast adaptation for different excitation and emission wavelengths. CCD camera, light source and synchronization are also under DaVis control. 9 2 Introduction 10 3 MITAS hardware 3.1 Mitas setup Arrange the MITAS system on a table and make sure that it has a safe standing position. x-y-drive light input port with fiber coupler optical fiber power supply plug x-y-drive motor connection cables Figure 3.1: MITAS input panel. 3.1.1 Cable connection Use the motor connection cables to connect the two terminals of the traversing table with the Motor 0 and Motor 1 sockets at the back of the MITAS controller. Note: To attach and release the MiniDIN quick connectors it must be unlocked by pulling the housing of the plug backwards (see fig. 3.2). It is automatically locked if it is plugged in. Connect the socket for the focus drive at the input side of the MITAS body with the receptacle labeled Motor 2 at the back of the MITAS controller. Use the RS232 connection cable to connect the Interface jack with the COM-port 11 3 MITAS hardware Figure 3.2: Unlocking the MinDIN quick connector. of the computer. Plug in the power supplies for the MITAS controller and for the MITAS. 3.1.2 Microscope lens assembly The FlowMaster MITAS can be operated with different microscope lenses depending on the required factor of magnification with standard RMS thread or M27 thread. There are adapters delivered with the system for these type of threads. To assemble the objective use the proper adapter. MITAS imaging performance with high sensitive Imager Intense camera: 12 Magnification 5X 10X 20X 40X 63X Numerical aperture (lens) 0.15 0.3 0.4 0.6 0.75 Field of view (system) µm2 1200×900 600×450 300×225 150×112 100×75 Depth of focus (lens) µm 28 7 4 1.7 1.1 Depth of focus (system) µm 46 16 6 2.5 1.5 Lateral resolution (system) µm 3.5 1.8 0.9 0.5 0.4 3.1 Mitas setup 3.1.3 End stop adjustment At the input panel of the MITAS are two sliders, where the end stop positions of the z-axis can be adjusted. To change the end stop position, loosen the screw in the center of the slider by 1-2 turns. Move the slider to the desired position and tighten the screw properly to fix the slider. Note: Avoid loosing the screw more than 2 turns or removing the screw. Adjust the end stop positions in a manner that the objective cannot touch the microscope table or the sample for any position of the table! Otherwise the objective or the table can be damaged! Always tighten the screw properly to secure the end stop position! Note: If end stop positions have been modified a new reference drive must be executed to update the maximum travel of the axes limited by the position of the end switches. See chapter 5.2.2 for details! Figure 3.3: adjustable end stop position 13 3 MITAS hardware 3.1.4 Camera assembly On the output side of the MITAS body is a flange to hold the camera. A special adapter to connect the camera to this flange is delivered with the system. Mount the adapter to the camera and assemble it to the MITAS body. Tighten the screws to hold the camera securely in place. The orientation of the camera is variable and depends on your application. camera flange Figure 3.4: MITAS output panel. 3.1.5 Light source assembly The MITAS system can be used with different light sources, such as flashlamps, DPSS lasers, diode lasers or Nd:YAG lasers. An optical fiber is used to deliver the light to the MITAS. Note: There are different types of fibers and couplers necessary depending on the laser. The standard light source is a DPSS laser. The fiber is delivered with two couplers. The input coupler connects the fiber to the laser. It will transfer the light into the fiber. The output coupler allows to fix the fiber to the MITAS. It must be mounted to the light input port of the MITAS. To assemble the fiber and couplers follow the instructions of the fiber manual. 3.2 MITAS controller The MITAS controller allows to operate the xyz traverse system manually by using a joystick or is controlled by the device control manager of DaVis. For cabling see paragraph 3.1.1. 14 3.2 MITAS controller The controller display (see fig. 3.5) shows the actual positions of all three axes. Figure 3.5: MITAS controller position display. The LEDs on the MITAS controller (see fig. ) are indicating the status of the system. power LED end switch LED speed indication LED Figure 3.6: MITAS controller status LED. If the power LED is illuminated, the controller is switched on. The joystick allows to move the traverse system. The x-y axis movement is controlled by tilting the joystick, the focus drive is controlled by rotation. All three axis can be moved simultaneously. The pushbutton on top of the joystick can be used to switch between slow and fast velocity of the drives. The speed indication LED is on if the velocity is fast, in slow mode the LED is off. The end switch LEDs are showing the status of the end switches. If one end switch is activated, the axis is only allowed to move into the direction away 15 3 MITAS hardware from the end switch. The stop button works as emergency stop button. It will stop all traverse activity immediately. After pressing the stop button the system must be restarted by switching the power button on the back of the controller off and on again. 3.3 Filter cube The filter cube exchange box allows a fast adaptation for different excitation and emission wavelengths. Figure 3.7: MITAS filter exchange box. To exchange the filter cube the box has to be pulled out. The filter cube can be released by detaching the screw. locking skrew filter cube filter exchange box Figure 3.8: MITAS filter exchange box with filter cube. Caution: The system must not be operated without a filter cube! For operation the filter exchange box with proper filter cube has to be slid in until sop. 16 3.4 Target LED 3.4 Target LED A white light target LED is built in. It can be used as pilot light and for focussing purposes. OFF ON Figure 3.9: MITAS target LED switch in off and on position. If the target LED switch is in horizontal position (tip is pointing toward the light input port) the LED is switched off and the light from the light input port is transmitted to the optics. If the switch is in 7.00 o’clock position the beam path from the light input port is blocked and the LED is switched on. The light from the LED is transmitted to the optics. 17 3 MITAS hardware 18 4 Operation Note: After the startup of the MITAS controller the axes are unable to move unless a reference drive is executed! Note: During the reference drive all axes are moved to all end switches. Remove all obstacles before starting the reference drive! See chapter 5.2.2 for details! 4.1 Focusing on micro structures Locating microstructures and focussing on micro channels can be challenging, especially with high magnification objectives. For this purpose the use of the built in target LED is recommended. Operate the camera at high quantum efficiency gain, set it to single frame single exposure mode. Use a exposure time of 1000-5000 µs. Switch off the laser. Switch on the target LED. The light spot of the target LED can be used as pilot light. Use the x-y traverse to move the light spot to the area of interest. If the position of the focus drive is changed, the spot size will change. The smallest spot size is the position of the focus. After this coarse adjustment the fine adjustment can be done by grabbing and observing the images of your microstructure online. If focus and y-x stage are on the desired position the pilot LED can be switched off. Now the system is ready for PIV , LIF or other measurements. 19 4 Operation 20 5 Software Settings 5.1 DaVis Hardware Setup To be able to control the Mitas on remote by DaVis it needs to be added as device to the DaVis Hardware Setup dialog. Additionally the camera needs to be provided with trigger signals that are synchronized to other devices, e.g. to a light source for illumination. Therefore a camera in a LaVision system is usually delivered with a Programmable Timing Unit (PTU). You can open the Hardware Setup dialog after start of the DaVis software if you login as Expert User and click on the Setup icon. 5.1.1 PTU If the PTU is already installed in the hardware setup you should find a Programmable Timing Unit in the device list as a sub-device under the Recording entry. If this item is not present in the device list click on the Recording item to highlight this and use the Add icon to select the Programmable Timing Unit (PTU) from the list of available hardware. Then click on the Programmable Timing Unit entry in the Recording tree and select Type: Version 9 on the card on the right hand side. 21 5 Software Settings 5.1.2 Timing Setup The settings on the Timing card need to fit to the experimental needs. For µPIV usually the Trigger based on: Time and Double events: T1 is preselected. In this case camera and light source are mapped to the reference times T1A and T1B. 5.1.3 Line Configuration Assuming that the system has been delivered with the standard #1002047 Camera adapter for PtuPortA and the standard #1000299 Laser adapter for PtuPortB the camera should be triggered using the Cam1 BNC and the laser should be triggered using the Q1 BNC. 22 5.1 DaVis Hardware Setup 5.1.4 PTU Light Source 1 Usually the MITAS system is delivered with the DPSS shuttered cw laser. In this case a PTU Light Source 1 is added as sub-device in the Recording entry. On the corresponding card the Type: Shuttered CW laser is selected and for PIV applications usually the Pulse type: double pulse is preselected with Pulse width at max. power: 100-500 µs and Maximum frequency: 30000 Hz. The max. pulse width and frequency need to be adjusted to the experimental conditions like the particle size and flow velocity. For the cameras Imager Intense, Imager Compact, Imager Pro X and Imager Pro Plus the exposure time of the first camera frame (in double frame mode) is automatically adapted to the laser pulse width. 5.1.5 Microscope The MITAS is added as Microscope sub-device in the Device entry. On the corresponding card the Type: MITAS and the suitable COM port for the RS232 communication need to be selected. 23 5 Software Settings For initialization and before DaVis is starting, the MITAS controller must be switched on. Otherwise the initialization fails and a error message is displayed. 5.2 Device Settings The MITAS can be operated with the joystick and with the device manager of DaVis. The main dialog of MITAS microscope contains a section where the traverse system can be operated. This is available in the Recording dialog if you press the Device button. Figure 5.1: If a button is pushed in the XY stage or Focus section the drive(s) is(are) moving in the specified direction by the values that have been selected on the Increments card (see below). There is another line where a step button is located. Pushing this button will move the drive by the value specified in the respective box. 24 5.2 Device Settings The Get position button will acquire the actual position from the controller. Pressing the Set as origin button will set the actual position to zero for all axis. The position list contains a number of positions. With the buttons below the position list a position can be added, edited or deleted. You have following options: Move to list position: Will move the traversing stages to the position that has been highlighted in the position list. Add list item: Will add the current position that is displayed in the X, Y, Z textboxes in the position list. You can add a label for the new position. Pressing the Transfer button will add this position to the list. Edit list item: Allows to edit the highlighted entry in the position list. Align item to position: Allows to realign the highlighted position in the position list if the system does not hit the original position. This can be especially the case after restart of DaVis and/or FlowMaster Mitas system and a new reference drive. The Align option needs to be executed for one entry of the position list only and will correct the offset automatically to all other entries. Delete list item: Will remove the highlighted position from the position list. Load item list: Saves all entries of the position list in a file. Save item list: Opens a position list that has been saved before. 5.2.1 Motor Settings The values on the Motor Settings card are predefined by LaVision and do not have to be changed at normal use. 25 5 Software Settings 5.2.2 Reference drive In the standard setup the MITAS system does not contain encoders for the position of the axes. If the controller is switched on the actual position is set to 0, 0, 0, the maximum travel of the axes limited by the positions of the end switches are unknown. Note: A reference drive is needed every time the controller was switched off or if end switches positions have been modified. Pushing the Start reference drive button the system will move all axes to all end switches. All axes are moved to their center position. This position is set to 0,0,0. This procedure needs to be applied every time you restart DaVis and/or the FlowMaster Mitas. If the Move to last origin checkbox is enabled the traverse system will move to the last origin, after finishing the reference drive. The values on the Reference drive card are predefined by LaVision and do not have to be changed at normal use. 5.2.3 Increments On the Increments card you may specify the values for the drive movement if you use the arrows on the Microscope MITAS card (see above). The software will move the corresponding drive with the distance that is specified in the small / little / large step size text box by one click on the short / middle / long arrow on the Microscope MITAS card. 26 5.2 Device Settings The values on the Increments card are predefined by LaVision and can be adjusted to convenient values for the experimental conditions. 27 5 Software Settings 28 Manual No 1003026 - Release October 10, 2008 LaVision We count on Photons LaVisionUK Ltd Downsview House/ Grove Technology Park Grove, Oxon, Ox 12 9FF/ UK www.lavisionuk.com Email: [email protected] Tel.: +44-(0)-870-997-6532 Fax: +44-(0)-870-762-6252 LaVision GmbH Anna-Vandenhoeck-Ring 19 D-37081 Goettingen, Germany www.lavision.com Email: [email protected] Tel.: +49(0)551-9004-0 Fax: +49(0)551-9004-100 LaVision, Inc. 301 W. Michigan Ave., Suite 403 Ypsilanti, MI 48197, USA www.lavisioninc.com Email: [email protected] Phone: +1(0)734-485-0913 Fax: +1(0)240-465-4306