Leica Dmi8 A Leica Dmi8 C Leica Dmi8 M

Transcript

Leica DMi8 A Leica DMi8 C Leica DMi8 M Installation Manual Leica Microsystems CMS GmbH • Manual 11934095, Revision 1.0 from 2015-01-15 Coyprights Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation. The information included in this manual may be changed without prior notice. Revision 1.0, published on 2015-01-15 by: Leica Microsystems CMS GmbH Ernst-Leitz-Strasse 17-37 D-35578 Wetzlar, Germany http://www.leica-microsystems.com Responsible for contents: Marketing CMS 2 Contents Contents 1. 1.1 Important Notes about this Manual........................... 4 Text symbols and their meanings................................ 4 2. 2.1 2.2 Safety Notes................................................................... 6 General safety notes..................................................... 6 Technical data................................................................ 6 3. Overview of Components............................................. 8 4. Unpacking..................................................................... 12 5. Assembling the Microscope..................................... 14 5.1 Assembling the DIC Module and DIC objective prisms............................................ 15 5.2 Assembling the specimen stage............................... 16 5.3 Inserting the eyepieces.............................................. 18 5.4 Inserting the objectives.............................................. 18 5.5 Assembling the incident light illuminator................. 19 5.5.1 Assembling the LED lamp housing............................ 19 5.5.2 Assembling the fluorescence illuminator................ 19 5.6 Equipping the incident light reflector turret............ 20 5.7 Assembling the polarizer and analyzer.................... 21 5.8 Assembling the transmitted light illuminator arm (TL)...................................................... 22 5.8.1 Tiltable transmitted light illuminator arm................. 22 5.8.2 Fixed transmitted light illuminator arm..................... 23 5.9 Assembling the condensers for the tiltable transmitted light illuminator arm..... 24 5.10 Assembling the transmitted light lamp housing...... 30 5.11 Wiring............................................................................. 31 5.11.1 Wiring for microscopes with transmitted light illuminator arm............................... 32 6. Startup........................................................................... 33 6.1  Adjusting the diaphragms for the incident light illuminator................................. 33 6.2 Inspecting the phase contrast ring .......................... 34 6.3 Setting the polarizer.................................................... 36 6.4 Condenser centering................................................... 37 7. Index.............................................................................. 38 3 1. Important Notes about this Manual 1. Important Notes about this Manual Caution! This Installation Manual is an essential component of the product; it must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference. This Installation Manual includes important instructions and information related to the operating safety and maintenance of the instrument. 1.1 Text symbols and their meanings (Fig. 1.2) → p.20 Numbers in parentheses, e.g. (Fig. 1.2), refer to illustrations, in the example to Fig. 1, Item 2. Numbers with an arrow, e.g. → p. 20, refer to a specific page of the manual. WARNING WARNING indicates a hazard with a medium degree of risk that, if not avoided, can result in death or serious injury. CAUTION CAUTION indicates a hazard with a low degree of risk that, if not avoided, can result in minor or moderate injury. Caution! In this manual, additional safety notes are indicated with the triangle symbol shown here, and have a gray background. ! 4 Caution! The instrument and accessories can be damaged when operated incorrectly. 1. IMPORTANT NOTES ABOUT THIS MANUAL Warning of hazardous electrical voltage! Danger of electric shock! Danger due to hot surface Warning of optical radiation! Never look directly into the light beam! Wear safety goggles! Warning of electromagnetic field Warning of UV radiation! Wear safety goggles! Warning of hazardous laser radiation – take appropriate safety precautions! Warning of hand injuries Instructions for disposing of the instrument, its accessories and consumables. Connection to ground Explanatory note * MM/YYYY Item not contained in all equipment configurations Manufacturing date, e.g. 11 / 2011 for November 2011 China RoHS 50 years EFUP (Environmentally friendly use period) 5 2. Safety Notes 2. Safety Notes 2.1 General safety notes 2.2 Technical data Compact Leica CTR electronics box Caution! Make sure to follow the safety notes in the Leica DMi8 User Manual. Caution! Also see the instructions and warnings contained in this Installation Manual. Caution! The instruments and accessories described in this Installation Manual have been safety-tested and checked for possible hazards. The responsible Leica representative or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual! Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims! 6 For indoor use only. Supply voltage: 100 – 240 VAC Frequency: 50 / 60 Hz Power consumption: max. 150 VA Fuses: 3.15 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 15° - 35°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 Advanced/Advanced+ Leica CTR electronics box For indoor use only. Supply voltage: 100 – 240 VAC Frequency: 50 / 60 Hz Power consumption: max. 290 VA Fuses: 6.3 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 15° - 35°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 2. Safety Notes Leica DMi8 M microscope (manual) Leica EL6000* For indoor use only. Supply voltage: 100 – 240 VAC Frequency: 50 / 60 Hz Power consumption: max. 55 VA Fuses: 1.6 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 15° - 35°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 For indoor use only. Supply voltage: 100 – 240 VAC Frequency: 50 / 60 Hz Power consumption: max. 200 VA Fuses: 2.5 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 0° - 40°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 (* See provided Instructions for Use) Leica DMi8 C microscope (manually encoded) For indoor use only. Supply voltage: 100 – 240 VAC Frequency: 50 / 60 Hz Power consumption: max. 55 VA Fuses: 1.6 A, slow-blowing, breaking capacity H, 250 VAC Size: 5x20 mm Ambient temperature: 15° - 35°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I Overvoltage category: II Pollution degree: 2 Leica DMi8 A microscope (automated variants) For indoor use only. Supply voltage: 100 – 240 VAC (→ Leica CTR) Frequency: 50 / 60 Hz (→ Leica CTR) Power consumption: see Leica CTR Fuses: see Leica CTR Ambient temperature: 15° - 35°C Relative humidity: 90% up to 30°C, non-condensing Protection class: I (→ Leica CTR) Overvoltage category: II (→ Leica CTR) Pollution degree: 2 (→ Leica CTR) 7 3. Overview of Components 3. Overview of Components 3 4 2 1 5 6 8 12 11 10 7 9 Fig. 1: Right side of the Leica DMi8 A 1 2 3 4 5 6 8 Objective turret with objectives Tube Eyepieces and eyepiece tubes Specimen stage LED lamp housing Colour filter slot 7 8 9 10 11 12 Aperture diaphragm Field diaphragm Polarizer slot Analyzer slot Cover for right side-port Cover for motorized incident light turret disk 3. Overview of Components 1 2 4 3 Fig. 2: Left side of the Leica DMi8 A 1 2 3 4 Motorized DIC objective prism disk Cover plate (not used) Service port Left side-port with camera 9 3. Overview of Components 1 2 3 10 9 8 4 5 7 Fig. 3: Left side of the Leica DMi8 C 1 2 3 4 5 6 7 8 9 10 10 Eyepieces and eyepiece tubes Tube Top-port with camera Cover plate (not used) Incident light turret disk Cover for left side-port Manual DIC objective prism disk LED lamp housing Objective turret with objectives Specimen stage 6 3. Overview of Components Variants with transmitted light illuminator arm Fig. 4: Left side of the Leica DMi8 with tiltable transmitted light illuminator arm 1 Tiltable transmitted light illuminator arm 2 Condenser height adjustment 3 Manual polarizer 4 Manual condenser, alternatively: motorized condenser 5 Condenser head 6 Condenser height stop 1 2 3 4 6 Fig. 5: Right side of the Leica DMi8 with tiltable transmitted light illuminator arm 1 Transmitted light filters 2 Transmitted light lamp housing receptacle 3 Condenser with motorized polarizer 5 1 2 3 Fig. 6: Left side of the Leica DMi8 with fixed transmitted light illuminator arm 1 Stop lever for condenser height adjustment 2 Fixed transmitted light illuminator arm 3 Transmitted light lamp housing 4 Receptacle for transmitted light filter 5 Light ring slide 6 Manual condenser with aperture diaphragm 3 4 2 1 5 6 11 4. Unpacking 4. Unpacking The microscope is delivered in several packages depending on instrument configuration and the scope of the system. The Leica CTR electronics box, Leica SmartMove control element, Leica STP4000/8000 control panel, movable stages/ stage accessories and Leica EL6000 compact light source are delivered in separate packages. Note: If at all possible, avoid touching optical surfaces such as the lens surfaces on the objective. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces in a short time. See the chapter on "Care of the Microscope"→ for additional instructions in the Instructions for Use. Caution! Caution! The microscope may only be set up, installed and put into operation by authorized Leica personnel! Caution! The microscope may only be operated by personnel who have been instructed by an authorized Leica employee! Please compare the contents of the package you receive carefully with the packing slip, the delivery note or the invoice. We highly recommend keeping a copy of these documents with the manual in order to have delivery date and delivery package information available when (for example) placing repeat orders or making service calls. Please make sure that no small parts are lost in the packaging materials. Some of our packaging materials have symbols to help in environmentally responsible recycling. First, carefully remove all components from the transportation and packaging materials. Caution! If an instrument or its packaging have visible damage, do not put the instrument into operation. 12 Do not connect the microscope and peripherals to the power supply at this point under any circumstances! Installation location Work with the microscope should be performed in a dustfree room, which is free of oil vapors, other chemical vapors and extreme humidity. At the workstation, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and longer microscopic scans. Permitted ambient conditions: Temperature 15–35°C Relative humidity maximum 90% up to 30°C non-condensing Microscopes in warm and warm-damp climatic zones require special care in order to prevent fungus contamination. For additional instructions, see the chapter on "Care of the Microscope" in the→ User Manual. Caution! The microscope must be set up so that the power voltage input is not obstructed and the instrument can quickly be removed from the power supply if necessary. 4. Unpacking Caution! Electrical components must be placed at least 10  cm away from the wall and away from flammable substances. Weight The weight of the microscope depends on the respective equipment configuration. CAUTION At a full load, the microscope weighs more than 18 kg. The user must take corresponding precautions for transporting the instrument. 13 5. Assembling the Microscope 5. Assembling the Microscope The microscope components in this order: +) are logically assembled • Only for microscopes with transmitted light illuminator arm (chapter 5.8-5.10): • Transmitted light illuminator arm • Condenser with condenser head • Transmitted light lamp housing • DIC module and DIC objective prisms • Specimen stage • Eyepieces • Objectives • Incident light illuminator (LED lamp housing) • Equipment for the incident light turret • Polarizer and analyzer When using other systems and advanced optical accessories, the sequence may vary. Only a few commonly used screwdrivers and keys are necessary for assembly; these are included in the delivery package. +) Depending on scope of delivery 14 5. Assembling the Microscope – DIC module and DIC objective prisms 5.1 Assembling the DIC Module and DIC objective prisms If the microscope is not equipped with DIC, please jump ahead to Chapter 5.2. In microscopes of the Leica DMi8 M series, the DIC prisms are already installed in the DIC disk (Fig. 7) below the objective turret. Motorized, manually encoded and manual DIC disks are offered. Assembly is identical for all variants. Fig. 7: DIC objective prism turret (encoded and motorized) 1 IC objective prism in mount 2 Identifying letter (ID) 3 Orientation pin When upgrading the IC prism disk, follow these steps: • Remove the front panel (Fig. 8) under the objective turret after loosening the Allen screws (Fig. 9). • Insert the DIC prism disk (Fig. 7) evenly into the receptacle. Start by gently turning one screw using the 3 mm hexagonal key provided. Then, tighten both Allen screws. ! Caution! 3 2 1 Fig. 8: Front panel for DIC prism disk Insert the prism disk so that the electronics board is facing downwards. Do not touch the electronics (especially the contacts) with your fingers! Upgrading individual IC prisms: • Loosen both Allen screws and remove the prism disk. Fig. 9: Removing the front panel • Place the prisms against the peg (Fig. 7.3) and the shim between the prism and screw (Fig. 10) and lightly tighten to avoid tension. Insert the prisms so that the identifying letter, or ID, is facing upwards and is legible. • After assembling the prisms, reinsert the prism disk into the receptacle. Alternatively: Insert the objective prism slide (Fig. 11) into the slot below the objective turret. The code letter must match the code letter on the objective. Fig. 11: Objective prism slide Fig. 10: IC objective prism 1 Objective prism in mount 2 Washer and screw 2 1 15 5. Assembling the Microscope – Specimen stages 5.2 Assembling the specimen stage Fig. 12: Fixed stage There is a wide variety of available specimen stages. The accompanying figures depict a few examples. The assembly of each of these stages is identical. The stages are permanently affixed to the microscope using 3 screws (Fig. 14). In the case of fixed stages, a mechanical stage can be attached (Fig. 15). ! Caution! Screw lengths may vary. The following applies to the shipment of different screws: Always use the shorter of the 3 screws for the front hole and both screws of equal length for the rear holes. Fig. 13: Mechanical 3-plate stage • Align the stage so that both holes point back towards the illumination axis and the single hole is facing in the direction of the tube. • Align the fastening holes in the stage with the holes in the contact surface. If the holes are not visible, as in the case of the 3-plated cross-stage or scanning stage, gently move the upper tabletop until the opening is visible. • Start by gently turning the front screw using the provided 3 mm hexagonal key. It is important that the shorter of the 3 screws is always placed in the front hole, because a screw that is too long can damage the focus hub. Fig. 14: a, b assembly screws for 3-plate cross-stage • Then, tighten both rear screws. • Finally, retighten the front screw. a 16 b 5. Assembling the Microscope – Specimen stages Fixed stage Fig. 15: Mechanical stage for fixed micromanipulation stage Mechanical stages for fixing holders for various culture flasks are also optionally available for fixed stage plates (Fig. 15). • The mechanical stage has 2 screws. Tighten these screws into the threaded holes on the bottom of the fixed stages using the 3  mm hexagon socket wrench and retighten regularly after frequent use of the mechanical stage. The mechanical stage is preadjusted at the factory. If the mechanical stage shifts out of focus when moving from right to left, this can be corrected by Leica Technical Service. Fig. 16: Inserts for mechanical stage (fixed stage) For additional examples, see special brochure • Now, remove the ordered stage insert or inserts (Fig. 16) from the packaging and guide one stage insert into the precise click system. The stage, mechanical stage and stage insert are now ready to use. Self-adhesive scales for reading the coordinate adjustment are included with some (not all) inserts. • Finally, affix these scales in the recesses of the mechanical stage. Motorized 3-plate or scanning stage 3-plate stages and scanning stages: • After assembling the stage, first connect (in the case of motorized stages) the provided stage cable to the socket on the stage and then to the electronics box. The corresponding mark on the box is called "XY- Stage". Various inserts are offered with the normal 3-plate or scanning stages (including heated inserts). • Carefully place these inserts into the corner provided with spring clips from above at an angle. The stage inserts click into place once positioned correctly. ! Caution! Only press on the spring clips from the sides. Never press on the insert plate at the spring clips from above at an angle, because then the insert will not be plane-parallel to the stage and cannot be bent. 17 5. Assembling the Microscope – Eyepiece and objectives 5.3 Inserting the eyepieces Fig. 17: Eyepieces • Insert the eyepieces into the eyepiece tubes. Note: We recommend using the LAS software to teach in eyepieces that are not included in the scope of delivery. This ensures that the total magnification specified on the touchscreen is correct. See LAS software manual: 5.4 Inserting the objectives • Screw the objectives into the objective turret. The receptacles on the objective turret are numbered (Fig. 18). Depending on your equipment, specific positions have already been assigned to the individual objectives at the factory. For details on the exact positions of the objectives, please refer to the enclosed "Identification sheet". Fig. 18: Objective turret ! Caution! Close vacant threads in the turret using dust protection caps! Please note that the front lenses of the objectives face upwards. This subjects them to increased exposure to contamination compared to objectives on upright microscopes. Check the front lens more often for cleanliness for this reason. Note: We recommend carrying out a parfocality adjustment via the LAS software. See LAS software manual: 18 Fig. 19: Objective turret, equipped 5. Assembling the Microscope – Illumination 5.5 Assembling the incident light illuminator 5.5.2 Assembling the fluorescence illuminator Leica EL6000 Caution! Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly. See separate manual. CAUTION CAUTION When using the Leica EL6000 compact light source, it is essential to observe the safety notes in the separate instructions included. Light sources pose a potential irradiation risk (blinding, UV radiation, IR radiation). Therefore, lamps may only be operated in closed housing and in an installed condition. Never look directly into the beam path (blinding hazard). CAUTION 5.5.1 Assembling the LED lamp housing • Place the lamp housing into the incident light lamp housing receptacle (Fig. 20) and fasten it with the side clamping screw. Leica DMi8 M with manual incident light method: • Connect the cable of the lamp housing to the port (Fig. 21.1) on the rear side of the microscope. Leica DMi8 A and C with motorized incident light method: • Connect the cable of the lamp housing to the power supply for transmitted light for the Leica CTR electronics box ("Lamp RL" port). Fig. 20: Fastening the lamp housing to the incident light axis 1 Fastening screw Just switch on the light source once the light guide is connected to the microscope. Uncontrolled light output from the light guide poses a blinding hazard! There is also a burn hazard at the light guide output! • Screw the Leica EL6000 microscope adapter onto the rear side of the microscope in the lamp housing receptacle. • Close the light guide according to the information in the Leica EL6000 manual. Fig. 21: Manually connecting the lamp housing for the Leica DMi8 1 Incident light lamp housing connection 1 1 19 5. Assembling the Microscope – Incident Light turret equipment 5.6 Equipping the incident light reflector turret ! Caution! Fig. 22: Access to turret disk 1 Cover First, completely read through this section before you begin equipping the reflector turret disk. The reflector turret disk can be accessed from both the right side and the left side of the microscope (Fig. 22). The positions on the reflector turret disk are numbered. Depending on how they are equipped, the individual filter and/ or reflector cubes are set in pre-assigned positions at the factory. For details, check the identification sheet included with your order. 1 Fig. 23: Equipping the turret disk • Remove the cover from the reflector turret disk (Fig. 22.1). The cover is secured in place by a magnetic holder and can be detached from the front. • With the holder facing you squarely, slide the filter cube or reflector cube forwards into the holder as described in the identification sheet provided. • Check to ensure that the cube is placed properly. A loose cube can be damaged when rotating the turret disk or can block the disk. • Once all filter blocks and reflector blocks have been inserted, put the cover back in place. Note: Is the microscope equipped with a 2-fold filter turret, the turret must be pushed across the arrester position to exchange the filter cubes. It is not possible to insert the filter cubes when the turret is in the arresting position. 20 Fig. 24: Turret disk with used filter cubes 5. Assembling the Microscope – Polarizer and analyzer 5.7 Assembling the polarizer and analyzer Manual incident light polarizer • Remove the plug cap on the right side of the stand (behind the incident light turret disk (Fig. 25.1)). • Insert the polarizer into the receptacle until it latches in place (Fig. 25.1). Fig. 25: 1 Incident light polarizer 2 Incident light analyzer Manual incident light analyzer • Remove the plug cap on the right side of the stand (below the incident light turret disk (Fig. 25.2)). • Insert the analyzer into the receptacle until it latches in place (Fig. 25.2). 1 2 21 5. Assembling the Microscope – Transmitted light illumination carrier 5.8 Assembling the transmitted light illuminator arm (TL) Fig. 26: Transmitted light illuminator arm assembly 1 Transmitted light illuminator arm 2 Pin on transmitted light illuminator arm 3 Contact surface 4 Groove on contact surface 5 Connecting cable 5.8.1 Tiltable transmitted light illuminator arm • Wipe off the microscope’s contact surface (Fig. 26.3) with a dry cloth. • Lightly tilt the illuminator arm (Fig. 26.1) to the rear and insert it so that the pin (Fig. 26.2) fits in the groove of the contact surface (Fig. 26.4). • Raise up the transmitted light illuminator arm and fasten it with 4 screws (Fig. 27.1). When fastening, do not hold onto the transmitted light illuminator arm. This ensures optimum alignment for the optical axis. 1 3 5 2 4 Fig. 27: Rear side of motorized Leica DMi8 1 Fastening screws on the transmitted light illuminator arm 2 Knurled ring for locking the transmitted light llluminator arm in place 3 EXT1-EXT6 sockets • The tilting angle of the illuminator arm can be modified or locked in place in the vertical position using the knurled screw (Fig. 27.2). Leica DMi8 A • Connect the electronics cable to one of the EXT1–EXT6 sockets (Fig. 27.3). 1 2 1 3 22 5. Assembling the Microscope – Transmitted light illumination carrier 5.8.2 Fixed transmitted light illuminator arm Assembling the transmitted light column Fig. 28: Condensers 1 S40/0.45 condenser 2 S80/0.30 condenser • Attach the transmitted light illumination column (Fig. 30.1) and fasten it with 4 screws (Fig. 30.2). Attaching the condensers • Screw the S80/0.30 condenser (Fig. 28.2) or S40/0.45 condenser (Fig. 28.1) onto the condenser holder of the (Fig. 29.2) transmitted light illuminator arm from below. Inserting the transmitted light illuminator arm 1 2 Fig. 29: Transmitted light illumination unit with condenser 1 Condenser 2 Condenser holder 3 Transmitted light illuminator arm 4 Transmitted light illumination column 5 Stop lever for condenser height adjustment 6 Labels • Insert the transmitted light illuminator arm into the column from above by pressing and holding down the stop lever for condenser height adjustment (Fig. 29.5). • Position the transmitted light illuminator arm (Fig. 29.3) onto the transmitted light illumination column based on the condenser being used (S40/0.45 or S80/0.30) (Fig. 29.4) and release the stop lever. (Also see the following note.) The marks (Fig. 29.6) pertain to a liquid height of 15 mm. For stands with double marks, the upper and lower lines represent a range with different liquid heights. • Make sure that the transmitted light illuminator arm is engaged. • Connect the electronics cable to the port (Fig. 30.3). Note: For the stands with 3-plate mechanical stages, position the illuminator arm 25 mm below the marks, as the illumination axis is installed 25 mm higher on an adapter than on the fixed stage. Note: 2 3 1 5 6 4 6 Fig. 30: Assembling the transmitted light illumination column 1 Transmitted light illumination column 2 Fastening screws 3 Cable connection 1 2 A screw on the transmitted light illumination column prevents the condenser from colliding with the specimen stage. If necessary, move the screw into the higher of the two openings, if using the S80/0.30 condenser. 3 23 5. Assembling the Microscope – Condenser 5.9 Assembling the condensers for the tiltable transmitted light illuminator arm Fig. 31: S1-S28 condenser base For the fixed transmitted light illuminator arm, see Chapter 5.8.2 → Page 23. All condensers from the Leica DMi8 series with a tiltable transmitted light illuminator arm are equipped with a 7x turret disk and can be individually equipped with the corresponding ring diaphragms for phase contrast (PH), dark field (DF) or IC prisms for transmitted light differential interference contrast (DIC). Usually the light rings and condenser prisms have already been inserted into the turret disk at the factory, eliminating the need for subsequent assembly. For more information, read → Page 28: Assembling condensers. Fig. 32: S1 condenser head Fig. 33: S28 condenser head Fig. 34: Phase rings Fig. 35: Condenser prisms 24 5. Assembling the Microscope – Condenser Assembling light rings • Switch the microscope off. Fig. 36: Condenser 1 Condenser door 2 Centering hole • Remove the condenser door (Fig. 36.1). The light ring is inserted into one of the large receptacles of the condenser disk featuring guide grooves. • Using the adjusting key, turn the right centering screw of the condenser disk (Fig. 37.2) all the way back. In order to prevent the condenser disk from rotating, insert the adjusting key (Fig. 37.2) into the left centering screw of the disk. This may protrude a maximum of 1 mm into the opening. Insert the light rings for phase contrast (marked with the identification numbers 0, 1, 2 and 3) and the S focal intercept of the corresponding condenser head) and DF diaphragm (marked with D for dark field and the S focal intercept of the corresponding condenser head) into the receptacle drill holes of the turret disk as follows: 1 2 Fig. 37: Opened condenser 1 Condenser clamp 2 Adjusting key 1 21 • Select an opening and check to ensure that both fastening screws are loose enough so that they are no longer protruding into the opening. Move the desired light ring opening into the beam path opening to adjust the screws. Now, you can turn the screws using both adjusting keys. • Now, pick up the special condenser clamp (Fig. 37.1). • If possible, install light rings 0 to 3 in ascending order. You will find numbering for the openings on the edge of the gear rim (4 large openings: 1-4; 3 small openings: 5-7). 25 5. Assembling the Microscope – Condenser • Using the condenser clamp, grip the light ring to be installed (the marking must be facing upwards and be legible), so that the cross piece of the light ring stands at the center of the clamp’s lug and the upper edge of the light ring is lying flat in the clamp holder. The numbering should point to the top of the clamp. The light ring can be grFiged by pressing down on the side prongs of the clamp (Fig. 38). • Two guiding ridges can be found on the bottom of the light rings. These ridges must fit into the two grooves in the opening. The light ring must be inserted (condenser clamp from above at a slight angle and at an angle of approx. 90° to the housing) so that the mount is grips under the spring clip of the holder (Fig. 38). ! Caution! Never press down on the spring clip. This can destroy the clip or put the light ring in an unstable position. Make sure that the light ring clicks into place (through rotation) and loosen the clamp. If necessary, carefully remove fingerprints (or dust) from the prism. • The light ring is precentered using the left centering screw. The right centering screw must never limit the range of movement. • Write down the number of the opening and the light ring designation so that the LAS software can be adjusted later. • Remove the adjusting key and close the condenser again. • Centering precision is achieved using the telescopic unit after the microscope has been switched off. For information on installing IC condenser prisms, continue reading. Otherwise, jump ahead to Page 28 Assembling condensers. 26 Fig. 38: Inserting the prism The designation must be visible when inserted and oriented to the center of the condenser disk. Otherwise, a DIC image cannot be produced. 5. Assembling the Microscope – Condenser Assembling IC condenser prisms • Switch the microscope off. • Remove the condenser door (Fig. 36.1). The prism is inserted into one of the large receptacles of the condenser disk featuring guide grooves. • Using the adjusting key, turn the right centering screw of the condenser disk (Fig. 37.2) all the way back. In order to prevent the condenser disk from rotating, insert the adjusting key (Fig. 37.2) into the left centering screw of the disk. This may protrude a maximum of 1 mm into the opening. • Using the condenser clamp, grip the prism to be installed (the marking must be facing upwards and be legible), so that the cross piece of the prism ring stands at the center of the clamp’s lug and the upper edge of the prism is lying flat in the clamp holder. The K2-K16 numbering should face towards the tip of the clamp. The prism can be grFiged by pressing down on the side prongs of the clamp (Fig. 38). ! Caution! Never press down on the spring clip. This can destroy the clip or put the prism in an unstable position. Make sure that the prism clicks into place (through rotation) and loosen the clamp. If necessary, carefully remove fingerprints (or dust) from the prism. • The prism is precentered using the left centering screw. The right centering screw must never limit the range of movement. • Write down the number of the opening and the prism designation so that the LAS software can be adjusted later. • Remove the adjusting key and close the condenser again. • Centering precision is achieved using the telescopic unit after the microscope has been switched off. • Two guiding ridges can be found on the bottom of the prisms. These ridges must fit into the two grooves in the opening. The prism must be inserted (condenser clamp from above at a slight angle and at an angle of approx. 90° to the housing) so that the mount is grips under the spring clip of the holder (Fig. 38). 27 5. Assembling the Microscope – Condenser Assembling condensers Assembly is the same for all S1 to S70 condensers (motorized or manual/encoded; not encoded for S40). Fig. 39: Assembling the condenser on the transmitted light illuminator arm • Loosen the Allen screws on the right side of the condenser holder. • Place the condenser on top of the locking pins of the transmitted light illuminator arm and then move the condenser to the corresponding height. Marks on the column and the condenser help you in finding the correct distance. • Tighten the Allen screw once you have reached the correct position. Fig. 40: Condenser mounted on transmitted light illuminator arm 28 5. Assembling the Microscope – Condenser Condenser heads Assembling the polarizer There are 4 different condenser heads available: Preconfigured at the factory. 1) S1/1.40 oil 2) S1/0.90 dry 3) S23/0.53 4) S28/0.55 Proceed as follows when performing upgrades: Condenser head 1 and 2: • Screw the spacer ring (Fig. 41.2) into the threaded hole on the bottom of the condenser base. Manual condenser: Motorized condenser: See the enclosed Assembly manual. • Screw the S1 condenser head into this spacer ring. Condenser head 3 and 4: • Screw the condenser directly into the condenser base. The S40 and S70 condensers are delivered complete with condenser heads, so no additional assembly is necessary. Fig. 41: Inserting the S1 condenser heads 1 Condenser base 2 Spacer ring 3 Condenser head • Fasten the 1-position or 3-position position holder onto the top of the manual condenser as necessary. The holder has a guide that must be inserted into the opening next to the screw thread. The holder must be positioned so that the polarizer to be inserted or the filter covers the opening of the condenser (Fig. 44). • Now, insert the polarizer or filter into the holder with the desired side facing upwards (λ: lambda and polarizer; POL: only polarizer). A clicking mechanism indicates tells you when it is positioned correctly. It must be possible to turn the polarizer slightly in the guide (approx. 30°) until it reaches both stops. Fig. 43: Condenser with motorized polarizer 1 2 3 Fig. 42: Inserting the S28 condenser head Fig. 44: Condenser with manual polarizer 29 5. Assembling the Microscope – Illumination 5.10 Assembling the transmitted light lamp housing Fig. 45: Fastening the lamp housing to the transmitted light illuminator arm 1 Fastening screw Caution! Ensure that the lamp housing has been disconnected from the power supply. Unplug the power plug and the power supply during assembly. CAUTION Light sources pose a potential irradiation risk (blinding, UV radiation, IR radiation). Therefore, lamps may only be operated in closed housing and in an installed condition. Never look directly into the beam path (blinding hazard). 1 Fig. 46: Wiring in the lamp housing (cable shaft) For the tiltable transmitted light illuminator arm: • Place the lamp housing into the transmitted light lamp housing receptacle (Fig. 45) and fasten it with the side clamping screw. • Route the cable into the cable shaft on the rear side of the transmitted light illuminator arm (Fig. 46). Leica DMi8 M and C with manual transmitted light method: • Connect the cable of the lamp housing to the port (Fig. 47.1) on the rear side of the microscope. Leica DMi8 A with motorized transmitted light method: • Connect the cable of the lamp housing to the power supply for transmitted light for the Leica CTR electronics box (“Lamp TL” port). 30 Fig. 47: Manually connecting the lamp housing for the Leica DMi8 1 Transmitted light lamp housing connection 1 5. Assembling the Microscope – Wiring 5.11 Wiring Leica DMi8 A WARNING Only use power cables or alternative cables with VDE/ HAR codes that minimally fulfill the following conditions: 3x0.75 mm2 and 10A/250V. The microscope can be operated with or without an electronics box depending on its configuration. Depending on the microscope configuration, different electronics boxes are available: • Leica CTR compact electronics box • Leica CTR advanced electronics box Note: The electronics box may not be used with any other stands. The serial number of the corresponding stand is noted on the rear side of the electronics box. Leica DMi8 M/C • Connect the lamp cable to the port (Fig. 48.1). • Connect the microscope to the power supply using the supplied power cable (Fig. 48.2). Fig. 48: R  ear side of Leica DMi8 M 1 Lamp cable connection 2 Socket for power cable • Connect the incident light illuminator port (Fig. 49.3) of the rear side of the microscope to the Lamp RL port (Fig. 50/ Fig. 51.1) on the electronics box using the lamp cable. • Connect the Microscope socket (Fig. 50/Fig. 51.4) to the rear side of the stand (Fig. 49.1). Use the 25-pin microscope cable for this. • If present, connect the Leica SmartMove control element or Leica STP4000/8000 control panel to one of the USB ports (Fig. 50/Fig. 51.3). • If necessary, connect the motorized stage to the XYStage socket (Fig. 51.6). • The connection for additional motorized components depends on the configuration. • After completing all of the assembly work, the electronics box is connected to the power supply using the power cable provided (Fig. 50/Fig. 51.5). Caution! To prevent the sockets from overheating, ensure that the plugs are inserted and screwed in correctly. Fig. 49: Rear side of Leica DMi8 A 1 CTR electronics box connection 2 EXT1-EXT6 connections 3 Incident light illuminator connection 1 2 3 1 2 31 5. Assembling the Microscope – Wiring 5.11.1 Wiring for microscopes with transmitted light illuminator arm Leica DMi8 M/C For the tiltable transmitted light illuminator arm: • Connect the lamp cable to the port (Fig. 48.1). Fig. 50: Rear side of compact Leica CTR electronics box 1 Connection for lamp cable for incident light 2 Connection for lamp cable for transmitted light 3 2 x USB 4 Microscope connection 5 Socket for power cable 1 For the fixed transmitted light illuminator arm: • Connect the transmitted light illuminator arm to the port (Fig. 48.1). 2 3 • Connect the microscope to the power supply using the supplied power cable (Fig. 48.2). 4 5 Leica DMi8 A • Connect the electronics cable of the transmitted light illuminator arm to one of the EXT1–EXT6 sockets (Fig. 49.2). • Connect the lamp cable of the transmitted light illuminator arm to the Lamp TL port (Fig. 50/ Fig. 51.2) on the electronics box. Fig. 51: Rear side of advanced Leica CTR electronics box 1 Connection for lamp cable for incident light 2 Connection for lamp cable for transmitted light 3 1 x serial, 2 x USB 4 Microscope connection 5 Socket for power cable 6 Motorized stage connection 3 6 4 5 32 1 2 6. Instrument Setup– Adjusting the diaphragms 6. Startup 6.1 Fig. 52: Adjusting the diaphragms (incident light) 1 Adjusting screws for moving the field diaphragm 2 Adjusting screws for moving the aperture diaphragm  djusting the diaphragms A for the incident light illuminator The following procedure is provided for incident light-bright field illumination. • Select an objective with moderate magnification (10x– 20x) and adjust the image. 2 1 • Activate the incident light axis if necessary. • Switch on the BF (= brightfield) or Smith reflector. Adjusting the Aperture Diaphragm • Place a specimen on the stage. • Use a flat specimen with good reflective properties, and a glass plate (specimen carrier) if necessary. • Bring the specimen into focus and adjust the light intensity. • Remove one eyepiece (the right one, for example). Adjusting the field diaphragm • Close the illuminated field diaphragm until the edge of the diaphragm appears in the specimen plane. • If the limits of the field diaphragm are not in the center of the field of view, move the position of the field diaphragm to the center with the two centering screws (Fig. 52.1) on the right side of the stand using a 3 mm Allen key. When centering, observe the position of the field diaphragm through the eyepieces. • Close the aperture diaphragm until the edge of the diaphragm appears in the exit pupil of the objective (aperture diaphragm plane). • If the image does not appear in the middle of the field of view, the aperture diaphragm must be moved into the exit pupil using the centering screws (Fig. 52.2). • Open the aperture diaphragm until the speciment appears in good contrast. • Open the light field diaphragm until it just disappears from the field of view. • This setting of the field diaphragm remains the same for all objectives. It is necessary to readjust the field diaphragm only when using eyepieces with different field numbers or when changing the magnification using a magnification changer, and for photography and TV imaging. Narrowing the field diaphragm normally improves the contrast. 33 6. Instrument Setup– Inspecting the phase contrast ring 6.2 Inspecting the phase contrast ring For the Leica DMi8 with tiltable transmitted light illuminator arm If your microscope is equipped for the use of phase contrast, the light rings that fit the objectives are built into the condenser. The light rings are already centered in the factory. As a result of transport and setup of the stand, however, in some cases centering maybe be required again. Therefore check the centering. Note: Every objective is assigned its own light ring in the condenser disk. Therefore, a check must be performed for each objective. Regular phase contrast with phase objectives When rotating in an objective that is suitable for phase contrast, the corresponding light ring is introduced automatically (motorized condenser) or you must manually swing the corresponding light ring inwards. Leica DMi8 with manual condenser: • If necessary, set the TL bright-field position or empty position on the fluo turret, or select the "DAPI-TL" filter cube. • Select the bright-field position on the condenser. Leica DMi8 with motorized condenser: • Select the PH contrast method (phase contrast). • Instead of an eyepiece, insert the focusing telescope (Fig. 53) into the observation tube. • Select the phase contrast objective with the lowest magnification. • Optimize the focus on the specimen. • Put the ring structure (Fig. 55a) by into focus by slightly loosening the clamping ring (Fig. 53.2) and moving the eye lens (Fig. 53.1). • Retighten the clamping ring. 34 Fig. 53: Focusing telescope 1 Adjustable eyelens 2 Clamping ring for fixing the focus position 1 2 6. Instrument Setup– Inspecting the phase contrast ring Leica DMi8 with manual condenser: • Select the light ring for your active objective on the condenser. Leica DMi8 with motorized condenser: • The correct light ring on the motorized condenser is automatically swung inwards. Fig. 54: Condenser centering 1 Centering holes 2 Height adjustment 3 Prisms and phase ring centering 2 1 2 1 3 • If the light ring and the phase ring are not shown as arranged in Fig. 55c, the light ring must be centered and congruent. • Insert the centering key through the openings provided Fig. 54.3) on both sides of the condenser. • Turn the centering key until the dark ring (phase ring in the objective) is congruent with the slightly narrower bright ring (light ring in condenser) (Fig. 55c). 3 Fig. 55: Centering procedure for phase contrast PH=phase contrast ring, LR=light ring a Condenser in bright field (BF) position b Condenser in phase contrast (PH) position Light ring (LR) not centered c Light ring and phase ring centered a b c Caution! When changing the objective, the centering key must no longer be in the corresponding openings. You can lock the condenser. PH LR • Repeat the process for all other phase contrast objectives. • Always remove the centering keys after the centering procedure. Note: You can find more information on the phase contrast method in Leica Science Lab. Link: http://www.leica-microsystems.com/science-lab/ 35 6. Instrument Setup– Setting the polarizer 6.3 Setting the polarizer For the Leica DMi8 with tiltable transmitted light illuminator arm Start by removing the specimen from the microscope stage. Fig. 56: Condenser with manual polarizer 1 Leica DMi8 with manual condenser: • Select the bright-field position on the condenser. • Slide the analyzer into the analyzer slot on the right side of the stand. • Swing the condenser head inward. • Rotate the polarizer until the optimum extinction position is reached. Leica DMi8 with motorized condenser and polarizer: • Select the POL method. If the analyzer is present on the fluo turret as an analyzer block, it will automatically swing inwards. A manual analyzer must be manually inserted. On motorized condensers with motorized polarizers, the polarizers move into position automatically. • Insert the centering key into the corresponding openings on the condenser (Fig. 57). • Set the optimum extinction (max. darkness). (The analyzer must be swung inwards.) • Remove the centering keys again. Place the specimen back onto the stage. 36 Fig. 57: Condenser with motorized polarizer 1 Centering holes on the condenser ! 6. Instrument Setup– Condenser centering 6.4 Condenser centering For the Leica DMi8 with tiltable transmitted light illuminator arm Set the Koehler illumination for your microscope (not available for S40 and S70 condensers). The condenser is also pre-adjusted in the factory. However, depending on how the condenser is disassembled and reassembled, it may be necessary to re-center the condenser in some cases. Therefore, check the condenser centering. Fig. 58: Condenser centering 1 Centering holes 2 Height adjustment 3 Prisms and phase ring centering 4 Clamping screw 2 1 1 3 • Adjust the image so it is as sharp as possible by carefully moving the condenser up and down (Fig. 58.2). Try this with different field diaphragm settings until you can see a clear, sharp image (Fig. 59.b). • To center the sharp image, insert the centering key into the corresponding openings on both sides of the upper portion of the condenser (Fig. 58.1). Move the image into the center of the field of view (Fig. 59.c). Then, open the field diaphragm until the image fills almost the entire image field. The black edges of the image should have the same distance from the outer edge of the field of view all the way around. If this is not the case, center the image again using the centering screws. Use the condenser height adjustment to bring the edges into focus. Open the field diaphragm until the image fills the entire field of view and the black edges have completely disappeared from the field of view (Fig. 59.d). 2 3 4 Fig. 59: Koehler illumination a Illuminated field diaphragm not focused, not centered b Illuminated field diaphragm focused, but not centered c Illuminated field diaphragm focused and centered Diameter is too small, however d Field diameter (light) = Field diameter (view) (Koehler illumination) a b c d Note: You can find additional information regarding the correct settings for Koehler illumination in Leica Science Lab. Link: http://www.leica-microsystems.com/science-lab/ 37 7. Index 7. Index Symbole 3-plate stages 17 A Adjusting the illuminated field diaphragm 33 Ambient conditions 12 Analyzer 21, 36 Analyzer slot 8 Aperture diaphragm 8, 11 C Camera 9, 10 Centering hole 25 Centering procedure for phase contrast 35 Checking the phase contrast rings 34 Colour filter 8 Condenser 11 Condenser base 24 Condenser centering 35, 37 Condenser clamp 25 Condenser head 24, 29 Condenser height adjustment 11 Condenser height stop 11 Condenser prisms 24 Condensers 23, 24, 28 Connection for fixed transmitted light illuminator arm 31 Cover for fluorescent turret disk 8 D DIC disk 15 DIC module 15 DIC objective prism disk 9, 10 DIC objective prisms 15 E EXT1-EXT6 sockets 22 Eyepieces 8, 10, 18 Eyepiece tubes 8, 10 F O Field diaphragm 8 Fixed micromanipulation stage 17 Fixed stage 17 Fixed transmitted light illuminator arm 11 Fluorescence illuminator 19 Focusing telescope 34 Objective prism slide 15 Objectives 8, 10, 18 Objective turret 8, 10 I IC condenser prisms 27 IC prism disk 15 IC prisms 15 Identification sheet 18, 20 Illuminator 19 Incident light reflector turret 20 Incident light turret disk 8, 10 Inserts 17 Installation location 12 K Koehler illumination 37 L Lamp cable connection 31 LAS software 18, 26 LED lamp housing 10, 19 Leica CTR electronics box 31, 32 Leica EL6000 19 Leica Science Lab 35, 37 Light guide 19 Light ring 25, 26, 35 Light ring slide 11 Light source 19 M Mechanical stage 17 P Packaging material 12 Parfocality adjustment 18 Phase ring 24, 35 Polarizer 8, 11, 21, 29, 36 R Reflector cube 20 S Safety Notes 6 Scanning stage 17 Service port 9 Setting the motorized polarizer 36 Side-port 8, 9 Specimen stage 8, 10, 16 Stop lever for condenser height adjustment 23 Symbols 4 T Technical data 6 Tiltable transmitted light illuminator arm 11 Top-port 10 Transmitted light column 23 Transmitted light filter 11 Transmitted light illumination column 23 Transmitted light illuminator arm 11, 22 Transmitted light lamp housing 11, 30 Transmitted light lamp housing receptacle 8 Tube 8, 10 W Weight 13 Wiring 31 Wiring in the lamp housing 30 38 www.leica-microsystems.com Copyright © Leica Microsystems CMS GmbH • Ernst-Leitz-Strasse • 35578 Wetzlar Germany 2015 • Phone (06441)29-0 • Fax (06441)29-2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH. Order Nos. of the editions in: English 11934095 Printed on chlorine-free bleached paper. III/15/M.H. Revision 1.0, issued 2015-01-15