Transcript
Light Delivery Systems for Optogenetics MIGHTEX Product Catalog and Selection Guide 2013
OPTICAL TECHNOLOGIES
Close-up of a „light addressable“ fruit-fly brain, Credit: Jay Brooks
Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics
Table of Content Introduction ····················································································································································· 3 Mightex’s Optogenetics Light Delivery Systems Overview ····················································································· 3 (1) Fiber-Coupled LED System for in vivo Optogenetics (2) Polygon400 Patterned Illumination System for ex vivo or in vitro Optogenetics Mightex’s LED Sources Portfolio ························································································································· 5 LED Controllers ················································································································································ 6 (1) LED Controller Product Summary and Selection Guide (2) SLA-1000-2 Manual and Analog LED Controller (3) SLC-MA04-MU Manual and Software LED Controller (4) SLC-AA02-US and SLC-AA04-US LED Controllers with Software and TTL Triggering (5) BLS-Series BioLED Light Source Control Modules Fiber-Coupled LED Sources ······························································································································10 (1) Fiber-Coupled LED Product Summary and Selection Guide (2) FCS-Series Single-Wavelength Fiber-Coupled LEDs (3) WFC-Series Multi-Wavelength Fiber-Coupled LEDs Lightguide-Coupled LED Sources ······················································································································12 (1) Single-Wavelength Lightguide-Coupled LED Sources (2) Multi-Wavelength Lightguide-Coupled LED Sources Polygon400 Spatiotemporal Patterned Illuminators for Optogenetics ·····································································14 (1) Polygon400 Overview (2) Polygon400 With Built-In LED’s (3) Polygon400 With External Light Sources (4) Microscope Adapters for Polygon400
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics Introduction Optogenetics is a technology that allows targeted, fast control of precisely defined events in biological systems, by delivering optical control signal at the speed (millisecond scale) and with the precision (cell type–specific) required for biological processing. Mightex has developed a range of Light Delivery Systems for optogenetics, as described below.
Mightex’s Optogenetics Light Delivery Systems Overview (1) Fiber-Coupled LED System for in vivo Optogenetics Fiber-coupled LEDs are capable of precisely delivering light to specific cell(s) of interest, without disturbing other cells in the vicinity. Moreover, multi-wavelength fiber-coupled LEDs enable one to switch wavelengths (e.g. 470nm and 590nm) of the light delivered to the cell(s) through the same fiber, without the need of any mechanical movement or to physically switch fibers. This will allow fast excitation and fast inhibition of the cell(s). Mightex’s FCS- and WFC-series fiber-coupled LED sources are designed for such in vivo optogenetics applications.
Control Signals
Manual
Analog
TTL
Software
- customer defined.
LED Controllers
Fiber-Coupled LED’s
SLA-1000-2
FCS-series
BLS-SAxx-US
SLC-MA04-MU
WFC-series
- with single or multiple LED’s coupled into one (1) output fiber, without any moving parts in the optical path. Up to 8x LED’s can be coupled into the same fiber. (Single LED)
(Up to 4x LEDs)
(Up to 8x LEDs)
Interchangeable fiber (e.g. 50/100/200µm) Standard SMA Connector
Rotary Joint
Fiber-Optic Cannula
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (2) Polygon400 Patterned Illumination System for ex vivo or in vitro Optogenetics For ex vivo or in vitro Optogenetics, microscopes are usually used in order to achieve the spatial resolution required to view individual cells, and patterned illuminators are needed in order to precisely control (i.e. activate/silence) the cells of interest without disturbing other cells within the microscope’s Field of View (FoV). Mightex’s Polygon400 Dynamic Spatial Illuminators are designed for such ex vivo and in vitro optogenetics applications.
LED Controller
Control Signals Software/PC
TTL
Light Sources LEDs
BLS-PLxx-US
Others
Lasers
*
TTL
USB
Polygon400 Patterned Illuminators
Integrated/ Built-in
Coupling Mechanism
Lightguide
Fiber
Φ ~ mm
Φ ~ 100m
DSI-x-xxx...
Key Features:
Microscope Adapters
For Upright Microscopes
DSI-CUBE-xx-UA
For Inverted Microscopes
DSI-RING– xx
DLP based Patterned Illuminator;
Simultaneous illumination of multiple areas of interest;
Customer-defined shape/size of illumination pattern;
Up to 4000 frames per second;
USB2.0 interface, easy to install;
TTL trigger input/output;
Adapters for microscopes;
Built-in LEDs, or external light sources via fiber or lightguide;
Intuitive/powerful software for spatial/temporal/spectral control.
590nm
470nm Polygon400 on an Upright Microscope
Polygon400 on an Inverted Microscope
* A BLS-PLxx-US LED controller is only needed when an external LED source is used with the Polygon400. Integrated/built-in LEDs have their own integrated LED controllers.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics Mightex’s LED Sources Portfolio As far as optogenetics applications are concerned, LEDs are able to simultaneously meet both the speed (~ms) and the spatial precision (i.e. cell/neuron specific) requirements. Compared with lasers, LEDs excel in many respects: they are cheaper, smaller, more reliable, and easier to control. In addition, there are a wide range of LED wavelengths available in the market, making it easy to find the best wavelengths to excite or inhibit neurons infected with various optogenetics tools, such as 470nm for channelrhodopsin (ChR2) and 590nm for halorhodopsin (NpHR). Mightex has developed the most comprehensive LED source solutions in the market. The chart below shows the wavelength portfolio of Mightex’s LED’s.
In order to satisfy the specific needs of various customer applications, Mightex offers an extensive range of beam formats with our LED solutions, including collimated, uniform, focused, divergent, fiber-coupled, and lightguide-coupled LED sources. Microscope adapters are also available, in order to enable customers to mount the LED sources onto their microscopes. Please refer to the diagram below on how to choose the best beam format (and microscope adapters) for your specific applications.
If you require multiple wavelengths for your application, Mightex also offers a range of multi-wavelength LED solutions, as below: (1) Wavelength-switchable LED's with collimated output beam (WLS-series). With an optional lightguide adapter, the output beam can also be coupled into a liquid lightguide; (2) Multi-wavelength fiber-coupled LED's (WFC-series); and (3) Multi-wavelength collimated LED's using beam combiners. With an optional lightguide adapter, the output beam can also be coupled into a liquid lightguide. Various microscope adapters are also available for customer who would like to couple the LED light into microscopes.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics LED Controllers In optogenetics research, scientists may have the need to control LED’s in one or more of the following ways: (1) manually (e.g. with a turning knob); (2) with TTL pulses; (3) with analog signals; and (4) with software. Mightex has developed a range of LED controllers with different methods of control, as described in details below:.
(1) LED Controller Product Summary and Selection Guide Keys: SLA-1000-2
Manual
SLC-MA04-MU
TTL
Analog
SLC-AA02-US / SLC-AA04-US
Software
BLS-SA02-US / BLS-SA04-US
Control Signals Number of Channels (per device) 1 Minimum Pulse Width
2
4
2/4
2/4
~1ms
1ms
20s
20s
Accepts TTL Input to Encode Output Pulse Pattern
No
No
Yes
Yes
Accepts TTL Input to Trigger Pre-defined Pulse Pattern
No
No
Yes
Yes
Manual Control via Turning Knobs
Yes
Yes
No
No
Accepts Analog Input Control
Yes
No
No
No
Software control via GUI / SDK
No
Yes
Yes
Yes
Support Arbitrary Output Waveform/Pattern
Via external analog input (0~5V)
Via custom program based on SDK
Easily definable via Mightex’s software GUI / SDK
Easily definable via Mightex’s software GUI / SDK
Note: (1) Each channel is capable of independently controlling an LED or a string of LEDs (all in sync with each other). For example, with a four (4)-channel LED controller, one can control up to four (4) LED’s INDEPENDENTLY. If however one needed to control more than four (4) LED’s, multiple LED controllers can be operated in parallel.
(2) SLA-1000-2 Manual and Analog LED Controller PART NUMBER AND ORDERING INFORMATION SLA-1000-2 SLA-1000-2 LED controller has two (2) channels, and each can be operated in one of the following two modes: - Manual Mode: The output current (in CW) can be adjusted manually via a turning knob, ranging between 0 and the Maximum Output Current stated below; - Analog Input Control Mode: The output current can be controlled via a 0~5V analog input. The maximum modulation frequency is ~1KHz. Each channel has a three (3) digit DIP switch (as shown in the picture on the right): the 1st digit is used to set the working mode, while the 2nd and 3rd digits are used to set the Maximum Output Current, which allows one to limit the maximum current that passes through the LED to match its current rating and prevent LED damage due to overdriving. There are three (3) current limit settings: 350mA, 500mA and 1000mA. Parameters Number of Channels Maximum Output Current (Settable via DIP Switches) Power Supply (provided by Mightex, 12V) Input Voltage, Vdc Maximum Output Voltage Analog Input Control Signal Dimensions Weight
1
DIP Switches
SLA-1000-2 2
A
2
3
B
DIP Switch Setting Instruction A: Control Mode Setting
B: Current Limit Setting
350mA / 500mA / 1000mA 9 ~ 24 V
Manual Mode
1
Imax = 350mA
2 3
(Vdc - 3.0) V 0~5 V 80mm (L) x 64.3mm (W) x 23.7mm (H) 60g
1
Analog Input Control Mode
Imax = 500mA
2 3 Imax = 1000mA
2 3
Note: SLA-1000-2 is a UNIVERSAL LED controller designed to drive LED’s of any made and model. It is NOT limited to just Mightex’s LED’s.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (3) SLC-MA04-MU Manual and Software LED Controller PART NUMBER AND ORDERING INFORMATION SLC-MA04-MU Mightex's SLC-MA04-MU four-channel universal LED controller is designed to drive a broad range of LED light sources, and it offers the flexibility for users to operate each LED channel independently, both manually and through software. The software comes with a user-friendly GUI that enables one to drive LEDs without the need to write any code. Furthermore, a full-featured SDK is provided in order for users who wish to write their own code and to integrate the LED controller into their own systems. 1. Manual Mode: Each of the four channels can be operated manually in CW mode using one of the four (4) “Channel” knobs. The LED controller also has a fifth knob (i.e. a “Global” knob) that enables one to adjust the output current of all the channels at the same time and with the same step size. Therefore, one can first set the intensities for the LED’s - independently - using the four “Channel” knobs, and then can increase/decrease the set intensities of all channels simultaneously using the “Global” knob. In order to prevent LED damage due to overdriving/overheating, the maximum output current of each LED channel can be set individually, via the software provided with the LED controller, to match the current rating of the LED connected to the channel.
To PC
2. Software Mode: In software control mode, each channel can be individually configured by the software to operate in one of the following three modes: a. Disable Mode: The channel is disabled, and its output is completely turned off. b. Normal Mode (or CW Mode): The output current is constant, which can be adjusted (using software) from 0mA to 1,200mA through the USB interface. c. Strobe Mode: A Pulse-Width-Modulated (or PWM) periodic strobe pattern is output from the channel, which can be activated by a software trigger. The strobe pattern may repeat indefinitely or for a preset number of cycles, depending on the software setting. The maximum frequency of the PWM strobe is 500Hz. Parameters Number of Channels Maximum Output Current (Settable via software) Power Supply (provided by Mightex, 12V) Input Voltage, Vdc Maximum Output Voltage Minimum Pulse Width Timing Resolution Output Current Resolution
SLC-MA04-MU 4 Up to 1200mA 12 ~ 24 V (Vdc - 3.0) V 1ms 0.1ms 1mA
Output Current Accuracy
±5 mA or ±1.0%, whichever is larger
Output Current Repeatability
±2 mA or ±0.5%, whichever is larger
Interface Device Per Computer* Dimensions Weight
USB2.0 16 180.5mm (L) x 180mm (W) x 34.5mm (H) 400g
* The LED controller’s software enables up to sixteen (16) SLC-MA04-MU LED controllers to be operated simultaneously by a single computer, leading to a maximum of 64 channels in total.
Note: SLC-MA04-MU is a UNIVERSAL LED controller designed to drive LED’s of any made and model. It is NOT limited to just Mightex’s LED’s.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (4) SLC-AA02-US and SLC-AA04-US LED Controllers with Software and TTL Triggering PART NUMBER AND ORDERING INFORMATION SLC-AA02-US or SLC-AA04-US Mightex's SLC-AA02-US and SLC-AA04-US universal LED controllers are designed to drive a broad range of LED light sources, and they enable one to operate each LED channel independently through software with a user-friendly GUI. Furthermore, a fullfeatured SDK is provided in order for users to write their own software and to integrate the LED controller into their own systems. The SLC-AA02-US and SLC-AA04-US LED controllers can also be controlled via TTL trigger pulses. There are two trigger modes (which are settable/selectable via the software): (1) one can pre-define a pulse sequence (or pattern) in software and load/save it to the LED controller’s non-volatile memory, and a TTL pulse can then be used to trigger/activate the pre-defined pulse pattern; or (2) one can pre-set the output current (or the LED’s intensity), and then use a sequence of TTL pulses to ‘encode’ (the timing of) the LED’s output pulse pattern. Since in the latter case, the LED’s output pulse pattern strictly follows the input TTL pulse patter, it is also called ‘Follower Mode’.
USB
RS232
With an SLC-AA02-US or an SLC-AA04-US LED controller, one can use up to 128 pairs of [current (mA), duration (μs)] data points to define the 'shape' of the waveform. This will allow one to define an 'Arbitrary Waveform' for the LED driving current and consequently the LED's optical output. Details see diagram below.
To PC
Parameters Number of Channels Maximum Output Current (Settable via Software) Power Supply (provided by Mightex, default 12V) Input Voltage, Vdc Maximum Output Voltage
SLC-AA02-US / SLC-AA04-US 2/4 1000mA in CW mode 3500mA in strobe mode 12 ~ 24 V (Vdc - 0.5) V
Minimum Pulse Width
20s
Timing Resolution
20s
Output Current Resolution
1mA
Output Current Repeatability
±1mA (or +/-0.2%)
Interface (Settable via a Flip Switch on the Back Panel)
USB2.0 or RS232
Arbitrary Waveform Definition Accepts TTL Trigger Device Per Computer* Dimensions Weight
Up to 128 points of [current (mA), time interval (s)] Yes Up to 16 201mm(L) x 147mm (W) x 40mm (H) 600g
* The LED controller’s software enables up to sixteen (16) SLC-MA04-MU LED controllers to be operated simultaneously by a single computer, leading to a maximum of 32 channels for SLC-AA02-US and 64 channels for SLC-AA04-US, respectively.
Note: SLC-AA02-US and SLC-AA04-US are UNIVERSAL LED controllers designed to drive LED’s of any made and model. They are NOT limited to just Mightex’s LED’s.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (5) BLS-Series BioLED Light Source Control Modules PART NUMBER AND ORDERING INFORMATION BLS-SA02-US, BLS-SA04-US, BLS-PL02-US, and BLS-PL04-US Mightex BLS-series BioLED light sources are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.
(Front View)
All standard Mightex LED light sources can be integrated into the BLS-series light source system. Furthermore, customers may choose optical heads with different wavelengths and formats to be integrated with the same control module. Multiple control modules can be ‘stacked’ in software to support more than 4 optical heads. The control module features a linear LED driver design that eliminates light intensity ripples and oscillations often observed when low-cost buckpuck nonlinear drivers are used. Clean and highly repeatable pulses are critical to quantitative experiments. Both CW mode and pulse modes are supported. Time resolution of the control module is 20s and light intensity can be adjusted with 0.1% increments. Each driving channel on the control module has its own TTL trigger input. Rising edge, falling edge, and follower mode are supported in the trigger mode.
(Back View)
The control module can be operated without being connected to a computer. Once pulse sequences are programmed and stored into the control module (by user through software), the light source can operate alone without a computer. All it needs is a TTL trigger signal to output the user-programmed pulse sequences. Mightex BioLED light sources come with a Windows-based operation software featuring an intuitive yet powerful graphic user interface. A software development kit (SDK) is also provided for user integration into environment such as Labview and Matlab.
(Back Panel)
BLS-PLxx-US BioLED control modules are specifically designed to synchronize LED’s with Mightex’s Polygon400 Patterned Illuminators, in order to support synchronization between external LED’s with custom pre-defined illumination patterns using external TTL trigger pulses. BLS-SA02-US Synchronization with Polygon Number of channels
BLS-SA04-US
BLS-PL02-US
No 2
Yes 4
2
Output Intensity Resolution
0.1%
Time Resolution
20s
External Trigger
TTL
Trigger Connector
BNC
Optical (LED) Head Connector Host Interface On-Device Memory Device Per Computer* Dimensions Weight
BLS-PL04-US
4
2-pin Aero Connector USB2.0 or RS232, selectable Yes Up to 16 213mm (L) x 156mm (W) x 73mm (H) 800g
* The LED controller’s software enables up to sixteen (16) BioLED controllers to be operated simultaneously by a single computer, leading to a maximum of 32 channels for BLS-SA02-US and 64 channels for BLS-SA04-US, respectively.
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics Fiber-Coupled LED Sources Mightex has developed two (2) catogories of fiber-coupled LED sources: (1) FCS-series single-wavelength fiber-coupled LED’s; and (2) WFC-series multiwavelength fiber-coupled LED’s. This section describes the fiber-coupled LED selection guide as well as the detailed specifications of the LED sources.
(1) Fiber-Coupled LED Product Summary and Selection Guide Features
FCS-Series
WFC-Series
Number of Wavelengths/LEDs
1
Up to 8
Wavelength Range (nm)
240~940nm, or white LEDs
240~940nm, or white LEDs
Output Fiber
Interchangeable (e.g. 50/100/200µm etc.)
Interchangeable (e.g. 50/100/200µm etc.)
Fiber Connector
SMA
SMA
Note: For wavelength availability, please refer to Mightex’s LED wavelength portfolio.
(2) FCS-Series Single-Wavelength Fiber-Coupled LEDs Mightex FCS-series single-wavelength fiber-coupled LED light sources employ the latest high-power LED technologies and a proprietary coupling optics to achieve maximum optical output power. Optical output is coupled into a fiber through a standard SMA fiber adaptor port (SMA fiber patch cords are sold separately). FCS series also features a locking electrical connector for secured connection. The onepiece machined housing features multiple mounting holes. All Mightex LED controllers can be used to drive the FCS-series light sources. PART NUMBER AND ORDERING INFORMATION -XXX
FCS Wavelength Code (Refer to table below)
Configuration Code (default: “000”)
Interchangeable fiber SMA connector
For example, FCS-0470-000 is a 470nm fiber-coupled LED with an SMA fiber connector.
For optogenetics applications, light intensity (i.e. power per unit area, in mW/mm2) is a more important measure than light power (mW) itself. The table below summarizes the output power (mW) and the intensity mW/mm^2) of typical Mightex’s FCS-series single-wavelength fiber-coupled LED’s. Peak Wavelength (nm) 1 365 385 400 420 455 470 505 530 590 617 625 656 680 740 780 850 870 940
Description UV 365nm UV 385nm 400nm 420nm Royal Blue Blue Cyan Green Amber Red-Orange Red Deep red Deep Red NIR NIR NIR NIR NIR
Wavelength Code 0365 0385 0400 0420 0455 0470 0505 0530 0590 0617 0625 0656 0680 0740 0780 0850 0870 0940
Output Power (mW) 2 4.6 4.6 5.1 2.0 5.5 6.3 2.7 1.6 0.9 5.1 5.1 5.1 1.1 2.8 1.7 3.1 1.1 3.1
Intensity (mW/mm^2) 146 146 162 64 175 201 86 51 29 162 162 162 35 89 54 99 35 99
Output power measured here!
Note: (1) Other wavelengths and white LED’s are also available; (2) Output power measured at the output tip of a 200µm 0.39NA fiber. Higher output power can be obtained under certain conditions and in “Intellipulsing” mode with a BLS-SA02-US or a BLS-SA04-US BioLED control module. Please email
[email protected] or call +1-925-218-1885 for details.
Related Products: LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US Fiber Patch Cords: FPC-0200-22-01SMA, FPC-0200-22-02SMA, FPC-0200-37-01SMA, FPC-0200-37-02SMA
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (3) WFC-Series Multiwavelength Fiber-Coupled LEDs Mightex’s WFC-series multi-wavelength fiber-coupled light sources are enabled by the latest LED technologies and Mightex’s proprietary beam combining/coupling optics. Up to eight (8) LEDs are coherently combined into a single multi-mode fiber with the highest efficiency practically possible. Optical output is coupled into a fiber through a standard SMA fiber adaptor port, and customers can use fibers with different core diameters with the WFC module. Each LED in the module can be powered individually or simultaneously, making the WFC-series a new class of light sources with a tunable spectrum.
Up to 4x LED’s
Up to 8x LED’s
In optogenetics, for example, one might want to launch 470nm to excite ChR2 in a neuron, and then fast (in sub-ms) switch to 590nm to activate NpHR in order to inhibit the same neuron. This can be achieved by using a WFC-H2-0470-0590-000 which enables sub-ms switching between 470/590nm through the same fiber, and this can all be done electronically (through software) and there is no need for any mechanical movement. PART NUMBER AND ORDERING INFORMATION WFC -
X S - Standard configuration
NNumber of wavelengths
XXXX 1st Wavelength code
XXXX -
...
XXXX -
XXX
2nd Wavelength code
… (repeat if applicable)
N-th Wavelength code
Internal use
H - High-power configuration
Output power measured here!
For example, WFC-H4-0400-0470-059-0656-000 is a high-power configuration WFC light source with 4 wavelengths of 400nm, 470nm, 590nm, and 656nm. Wavelength1 (nm)
Wavelength Code
365 385
2
Output Power (mW) / Intensity (mW/mm^2) 2-wavelength
3-wavelength
4-wavelength
5-8 wavelength
0365
3.8mW (120mW/mm^2)
3.6mW (115mW/mm^2)
3.4mW (108mW/mm^2)
3.1mW (98 mW/mm^2)
0385
3.8mW (120mW/mm^2)
3.6mW (115mW/mm^2)
3.4mW (108mW/mm^2)
3.1mW (98mW/mm^2)
400
0400
3.5mW (110mW/mm^2)
3.3mW (105mW/mm^2)
3.1mW (100mW/mm^2)
2.8mW (90mW/mm^2)
420
0420
1.3mW (40mW/mm^2)
1.2mW (38mW/mm^2)
1.1mW (35mW/mm^2)
1.0mW (33mW/mm^2)
455
0455
4.4mW (140mW/mm^2)
4.2mW (133mW/mm^2)
4.0mW (128mW/mm^2)
3.5mW (113mW/mm^2)
470
0470
4.7mW (150mW/mm^2)
4.5mW (143mW/mm^2)
4.2mW (135mW/mm^2)
3.8mW (123mW/mm^2)
505
0505
2.2mW (70mW/mm^2)
2.0mW (65mW/mm^2)
2.0mW (63mW/mm^2)
1.8mW (58mW/mm^2)
530
0530
1.9mW (60mW/mm^2)
1.8mW (58mW/mm^2)
1.7mW (55mW/mm^2)
1.5mW (48mW/mm^2)
590
0590
1.0mW (33mW/mm^2)
0.9mW (30mW/mm^2)
0.9mW (30mW/mm^2)
0.9mW (28mW/mm^2)
617
0617
4.1mW (130mW/mm^2)
3.8mW (123mW/mm^2)
3.7mW (118mW/mm^2)
3.3mW (105mW/mm^2)
625
0625
4.8mW (153mW/mm^2)
4.6mW (148mW/mm^2)
4.3mW (138mW/mm^2)
3.9mW (125mW/mm^2)
656
0656
4.1mW (130mW/mm^2)
3.8mW (123mW/mm^2)
3.7mW (118mW/mm^2)
3.3mW (105mW/mm^2)
740
0740
1.9mW (60mW/mm^2)
1.8mW (58mW/mm^2)
1.7mW (55mW/mm^2)
1.5mW (48mW/mm^2)
850
0850
2.5mW (80mW/mm^2)
2.4mW (75mW/mm^2)
2.3mW (73mW/mm^2)
2.0mW (65mW/mm^2)
870
0870
1.9mW (60mW/mm^2)
1.8mW (58mW/mm^2)
1.7mW (55mW/mm^2)
1.5mW (48mW/mm^2)
940
0940
2.5mW (80mW/mm^2)
2.4mW (75mW/mm^2)
2.3mW (73mW/mm^2)
2.0mW (65mW/mm^2)
Note: (1) Other wavelengths and white LED’s are also available; (2) Output power measured at the output tip of a 200µm 0.39NA fiber. Higher output power can be obtained under certain conditions and in “Intellipulsing” mode with a BLS-SA02-US or a BLS-SA04-US BioLED control module. Please email
[email protected] or call +1-925-218-1885 for details.
Related Products: LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US Fiber Patch Cords: FPC-0200-22-01SMA, FPC-0200-22-02SMA, FPC-0200-37-01SMA, FPC-0200-37-02SMA
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics Lightguide-Coupled LED Sources (1) Single-Wavelength Lightguide-Coupled LED Sources Mightex GCS-series high-power LED sources are designed for high-efficiency coupling of LED light into a liquid lightguide or a fiber optic bundle. Virtually all lightguides with core diameters ranging from 3mm to 8mm can be used with the GCS-series light source. GCS series are designed as a universal light source for general lab use and OEM applications. Multi-chip LED emitters have been added to the product portfolio (Type-B), and some of these 7W to 15W Type-B LEDs have total output optical power quadrupling the power of a single-chip LED (Type-A). Type-B models feature a cooling fan, and have a different form factor compared to Type-A models. Power supply for the cooling fan is included in the price of the Type-B collimated LED sources, while Type-A LED’s do not need a cooling fan.
Type-A
PART NUMBER AND ORDERING INFORMATION
-
GCS Wavelength Code (Table-1)
- XXXXX
Electrical Power (W)
Lightguide Adapter Code (Table-2)
For example, GCS-0470-15-A0510 is a 15W 470nm light guide coupled LED source with a standard adapter for Mightex 3mm liquid lightguide (with 5mm ferrule diameter).
Type-B Table-1: Performance Specifications Part Number1
Wavelength Code
Type
Description
Peak Wavelength (nm)
Iop (mA)
Vop (V)
Radiant Flux2 (mW)
GCS-0365-02-xxxxx
0365
A
UV 365nm, 2W
365
500
3.8
90
GCS-0365-07-xxxxx
0365
B
UV 365nm, 7W
365
500
15
300
GCS-0385-02-xxxxx
0385
A
UV 385nm, 2W
385
500
3.8
100
GCS-0385-07-xxxxx
0385
B
UV 385nm, 7W
385
500
15
330
GCS-0455-04-xxxxx
0455
A
Royal Blue, 4W
455
1000
3.9
165
GCS-0470-04-xxxxx
0470
A
Blue, 4W
470
1000
3.9
130
GCS-0470-15-xxxxx
0470
B
Blue, 15W
470
1000
15
400
GCS-0505-04-xxxxx
0505
A
Cyan, 4W
505
1000
3.9
30
GCS-0530-04-xxxxx
0530
A
Green, 4W
530
1000
3.9
60
GCS-0530-15-xxxxx
0530
B
Green, 15W
530
1000
15
180
GCS-0590-03-xxxxx
0590
A
Amber, 3W
590
1000
3.2
35
GCS-0617-03-xxxxx
0617
A
Red-Orange, 3W
617
1000
3.0
200
GCS-0617-10-xxxxx
0617
B
Red-Orange, 10W
617
1000
10.8
600
GCS-0625-03-xxxxx
0625
A
Red, 3W
625
1000
3.0
200
GCS-0850-02-xxxxx
0850
A
NIR, 2W
850
1000
2.1
125
GCS-0940-02-xxxxx
0940
A
NIR, 2W
940
1000
2.4
125
GCS-3400-04-xxxxx
3400
A
Warm White, 4W
3400K
1000
3.9
95
GCS-5500-04-xxxxx
5500
A
Cool White, 4W
5500K
1000
3.9
95
GCS-6500-04-xxxxx
6500
A
6500K
1000
3.6
95
GCS-6500-15-xxxxx
6500
B
Glacier White, 4W Glacier White, 15W
6500K
1000
15
300
Note: 1. “xxxxx” is the Lightguide Adapter code, see Table-2 below; and 2. Measured at exiting end of a 3mm-core 0.59 numerical aperture (NA) liquid light guide.
Table-2: Lightguide Adapters Adapter Code
For Ferrule Diameter (mm)
For Ferrule Length (mm)
A0510
5
≥10
A0610 A0710
6 7
≥10 ≥10
Related Products: LED Controllers: SLA-1000-2, SLC-MA04-MU, SLC-AAxx-US, BLS-SAxx-US
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1212
Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (2) Multi-Wavelength Lightguide-Coupled LED Sources A Multi-Wavelength Lightguide-Coupled LED Source can be constructed using the following Mightex’s parts: (1) LCS-series collimated LED sources with wavelengths of customer’s choice; (2) appropriate Mightex’s LCS-BCxx series multi-wavelength beam combiners; (3) connecting plates that bind the beam combiners together; and (4) a lightguide adapter that focuses a collimated beam into a lightguide. For details, please see schematic diagram below.
Lightguide
Beam Combiner A four (4) wavelength lightguide-coupled LED source based on LCS-series collimated LED’s.
Connecting Plates
Lightguide Adapter
An LCS-series collimated LED consists of a collimating lens and a LED emitter. The LED emitter is placed at the focal plane of the collimating lens which images the LED emitter into infinity. The LED emitter is mounted directly onto the metal base of the collimator which also features an integrated heat sink. The light sources can be driven by Mightex LED controllers, or other LED controllers and current sources. An optional focusing module can be mounted on the front of the LED collimator to focus light into a tight spot which is an image of the LED emitter. One of the applications with the focusing module is to couple LED light into a fiber or a light guide.
LCS-series collimated LED sources
Mightex’s LCS-BCxx series multi-wavelength beam combiners can be used to combine two LCS-series collimated LED’s of different wavelengths into a single collimated beam. Multiple combiners can be cascaded to combine more than two collimated LED sources. At the heart of the beam combiner is a high-performance dichroic mirror that combines two wavelengths with >95% efficiency. Beam combiners are characterized by the edge wavelengths of their dichroic mirrors. Please use the following table to select the correct beam combiner for intended LCS-series LED sources. Part Number
Reflection Port
%R
Transmission Port
%T >95
LCS-BC25-0380
365nm
>95
385, 455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, 850, 870 or 940nm
LCS-BC25-0400
365 or 385nm
>95
455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, 850, 870 or 940nm
>95
LCS-BC25-0410
365, 385, or 400nm
>95
455, 470, 505, 530, 590, 617, 625, 657, 680, 740, 780, or 850nm
>95
LCS-BC25-0460 LCS-BC25-0480 LCS-BC25-0495 LCS-BC25-0505 LCS-BC25-0515 LCS-BC25-0520 LCS-BC25-0550 LCS-BC25-0560 LCS-BC25-0595 LCS-BC25-0605 LCS-BC25-0635 LCS-BC25-0660 LCS-BC25-0685
400nm 365, 385, 455, or 470nm 400, 455 or 470nm 455 or 470nm 365, 385, 455, 470, or 505nm 455, 470 or 505nm 365, 385, 455, 470, 505, or 530nm 470, 505 or 530nm 505 or 530nm 365, 385, 455, 470, 505, 530, or 590nm 470, 505, 530, 590, 617, or 625nm 590, 617 or 630nm 590, 617, 630 or 657nm
>95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95
470, 505, 530, 590, 617, 625 or 657nm 505, 530, 590, 617, 625, 657, 680, 740, 780, or 850nm 530, 590, 617, 625 or 657nm 530, 590, 617, 625, 657 or 740nm 530, 590, 617, 625, 657, 680, 740, 780, or 850nm 590, 617, 625, 657 or 740nm 590, 617, 625, 657, 680, 740, 780, or 850nm 590, 617, 625, 657 or 740nm 617, 625, 657, 740 or 850nm 617, 625, 657, 680, 740, 780, or 850nm 657, 740, or 850nm 740 or 850nm 740 or 850nm
>95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95 >95
LCS-BC25-0800
455, 470, 505, 530, 590, 617, 625, 657, 680, 740, or 780nm
>95
850, 870 or 940nm
>95
LCS-BC25-0810
740 or 780nm
>95
850 or 940nm
>95
LCS-BC25-0000
400, 455, 470, 505, 530, 590, 617, 630, 657, or white
~45
400, 455, 470, 505, 530, 590, 617, 630, 657, or white
~45
LCS-BC25-0070
400, 455, 470, 505, 530, 590, 617, 625, 657, or white
~30
400, 455, 470, 505, 530, 590, 617, 625, 657, or white
~70
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics Polygon400 Spatiotemporal Patterned Illuminators for Optogenetics (1) Polygon400 Overview Spatially patterned illumination with temporal and spectral control enables numerous new techniques in life-science applications such as optogenetics. For example, in optogenetics, selected neurons in a specimen slice can be activated or silenced with a user defined illumination pattern. Mightex Polygon400 multi-wavelength patterned illuminator integrates the state-of-the-art spatial light modulators and high-power LEDs using a proprietary Etendue-preserving optical design to deliver high-intensity illumination patterns with diffraction-limited resolution. A Texas Instruments’ DLP spatial light modulator is used to display a user defined image pattern. At the heart of the Polygon400 is a unique optical system that carefully delivers light from LED sources or an external light source (such as a laser or an arc lamp, throught an optical fiber or a lightguide) to the DLP panel and then through a microscope to the specimen plane. Such a systematic approach makes it possible to achieve maximum optical intensity while maintaining diffraction-limited imaging performance. Temporal performance is a key to many intended applications for Polygon400. With a frame rate of more than 4,000 fps and fastswitching LEDs, Mightex Polygon400 can deliver illumination patterns with micro-second precision. Advanced DSI models with external LED controllers also enable wavelength switching in between illumination patterns at the highest frame rate. A dedicated software allows users to generate illumination patterns as well as control illumination intensity and timing. The software also supports alignment between illumination patterns and images acquired through any digital cameras on a microscope. PART NUMBER AND ORDERING INFORMATION TTL Trigger-In
DSI-
xI - with built-in LEDs G - with lightguide input
xxxx1st wavelength code
xxxx-
xxxx-
2nd wavelength code
3rd wavelength code
TTL Trigger-Out
xxx Reserved for internal use
F - with fiber input Not applicable for lightguide/fiber based Polygon models
For example, DSI-I-0470-0590-000 is a Polygon400 module with two built-in LED sources: 470nm and 590nm. DSI-G-000 is a Polygon400 module with a lightguide as input light source.
Projection Area (FOV) and Pixel Resolution (at 1X magnification) Projection Area Height (mm) Width (mm) Diagonal (mm) Pixel size (m)
Leica 8.7 15.5 17.7 18.0
Nikon 8.7 15.5 17.7 18.0
Common Microscopes Olympus 7.8 13.9 16.0 16.2
Zeiss 7.2 12.7 14.6 14.8
Note: To calculate illumination area and pixel resolution at the specimen, simply divide the above numbers by the magnification of the objective. For example, under a 10x objective on an Olympus microscope, the illumination area will be 0.78mm x 1.39mm with a pixel resolution of 1.62m.
Control and Timing Parameters Allowed bitmap depth Exposure time @ 1bit Frame rate @ 1bit Exposure time @ 4bit Frame rate @ 4bit Exposure time @ 8bit Frame rate @ 8bit Input trigger Input trigger delay Output trigger Output trigger delay
System and Communication USB2.0 interface. 5Vdc 3A input power.
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Minimum 1 0.25 1.6 8.3 -
Maximum 8 2,000 4000 20 625 20 120 TTL, BNC connector
-
50 TTL, BNC connector User Programmable
Unit bit ms 1/sec ms 1/sec ms 1/sec s ms
Windows 7 (recommended), Windows XP Service Pack 2 or greater Screen resolution of 1366x768 or higher
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Light Delivery Systems for Optogenetics Light Delivery Systems for Optogenetics (2) Polygon400 With Build-In LED’s A Polygon400 unit may contain up to three (3) built-in LED’s, each of which can be operated sequentially (in Color Image Mode) or individually (in Pattern Sequence Mode). In this case, LED controllers are integrated into the Polygon400’s control electronics residing inside the metal housing, and hence there is no need for external LED controllers. Available LED Wavelengths and Output Power on the Specimen Under Microscope (in Pattern Sequence Mode) Wavelength (nm)
Output Power1(mW)
400 470 530 590 617 630 656
14.0 15.0 4.5 3.0 14.0 14.0 14.0
1. These are estimated power at specimen under a 20x 0.75NA Olympus objective.
(3) Polygon400 With External Light Sources
BioLED Control Module P/N: BLS-PL04-US
Polygon400 can also work with external light sources (e.g. lasers, arc lamps, and external LED sources etc) through a lightguide or an optical fiber. Two additional models of Polygon400 have been developed for this purpose: one (i.e. Polygon400g) with an lightguide input port, and the other (i.e. Polygon400f) with a fiber input port. Different Light Sources for Polygon400 Polygon400 (standard)
Polygon400g P/N: DSI-G-000
Polygon400f P/N: DSI-F-000
Light Source Input Port
N.A. (integrated LED’s)
Lightguide
Optical Fiber
LED as Light Sources
Yes
Yes
Yes
Laser as Light Sources
No
Yes
Yes
Hg Arc Lamp
No
Yes
No
Other Light Sources
No
Yes
No LED#3 (LCS-xxxx)
Polygon400g (P/N: DSI-G-000)
LED#2 (LCS-xxxx)
LED#4 (LCS-xxxx) Lightguide P/N: LLG-03-59-340-0800-1
LED#1 (LCS-xxxx) Mightex Multi-Wavelength Lightguide-Coupled LED
(4) Microscope Adapters for Polygon400 Mightex Polygon400 is designed to be easily inserted into the infinity path of a microscope. For inverted microscopes, the preferred inserting point is usually the back port of the microscope where a fluorescence attachment is commonly placed. A filter cube is required to fold the Polygon400’s light path into the microscope. The filter cubes used for fluorescence observation serves this purpose well. For upright microscopes we provide a beam combiner cube to be inserted below the binocular/ trinocular unit. The dichroic or mirror in the beam combiner directs the Polygon400 beam into the microscope light path.
(Left - Polygon400 beam combiner cube for upright microscopes; and, right adapter ring for inverted microscopes.)
Microscope Adapters for Polygon400 Leica
Nikon
Olympus
Zeiss
Upright
DSI-CUBE-LC-UA
DSI-CUBE-NK-UA
DSI-CUBE-OL-UA
DSI-CUBE-ZS-UA
Inverted
TBD
DSI-RING-NK-TI
DSI-RING-OL-IA
TBD
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