Micr249 - Catalog For Testing

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MICR249 Clinical & Laboratory Microscopes ECLIPSE 50i/55i . . . magine microscopy in perfect comfort / ECLIPSE i-Series Website www.nikon-i.com MICR249 Imagine the ultimate in comfortable microscopy operation; so comfortable, that you would not even be aware you were carrying out essential observations! After carefully listening to customer needs, Nikon designed the new 50i/55i series of compact, versatile microscopes that are ideal for clinical/laboratory inspection and basic research study. The ultimate in usability and comfort, the 50i/55i series incorporates a host of stunning features that take the stress out of microscopy. A stay-inposition stage handle and tilting/telescoping ergonomic eyepiece tube ensure ideal viewing posture, enabling long hours of observations to be carried out in perfect comfort. The 50i/55i series is built around Nikon’s tried and tested infinity optics, the CFI60 system, which means you not only get the optimum optical performance, but you also have the freedom to add various accessories to create the setup that best suits your purpose. . . . the ultimate comfort that takes clinical microscopy to new heights. 1 — Cool illumination (55i)—LED illumination generates no heat and maintains the color temperature even when the brightness is changed. Also, because this model can run on batteries, it can be used anywhere. (See page 4) — Novel ergo stage—the stage handle always remains in the same position during stage movement, enabling easy operation with your hand resting on the desk. (See page 4) — A digital camera can be mounted on the ergonomic eyepiece tube by using a DSC port, enabling easy digital documentation in a comfortable operating posture. (See page 6) 1 — The “Ergo-View,” a retrofittable compact cytodiagnostic unit, enables quick magnification changeover using a hand switch. (See page 7) / MICR249 . . . in harmony with you Cool illumination (55i) Utilizing white LED illumination, the 55i is an ideal choice for brightfield applications. As the color temperature does not change even when the brightness is altered, adjusting the color balance filter or voltage is no longer necessary. And because the LED illuminator is built into the base, comprehensive Koehler illumination using a diaphragm is possible. The illuminator does not generate heat. Due to its minimal power consumption, the 55i has an extremely long lamp lifecycle, and its battery drive means it can be used anywhere. Easy-access controls Refined stage Stay-in-position stage handle The stage handle stays at the same position without interfering with the stage movement. Because the stage handle and the focusing knob are always situated close to each other, users can easily and smoothly operate the controls with their hand resting on the desk. The height and torque of the stage handle are adjustable to further enhance comfort during operation. Frequently used controls and switches for adjusting the field diaphragm and illumination intensity have been concentrated in the lower part of the righthand side to minimize the operator’s hand movements and enable operation without having to take your eyes off the specimen. — With white LED illumination as its light source, this model is perfect for brightfield applications. — No color temperature deviation even when brightness is changed. — Cool LED prevents focus deviations caused by heat-deformed equipment. — When Ergo-View cytodiagnostic unit is installed, it maintains uniform brightness even if magnifications are changed. — Long lifecycle illumination consumes very little power. Rock-solid stability New ergonomic tube The new ergonomic tube can be inclined from 10° to 30° and the eyepieces can be extended 40mm. This ensures optimum eye point and a comfortable viewing posture, regardless of the operator’s physique or if intermediate modules are being used. An optional DSC port for attaching a digital camera enables users to create a digital documentation system in comfort. An optional eye-level riser* can raise the eye point in 25mm increments. *The number of risers that can be used at any one time depends on the intermediate modules being used. Viewing angle is adjustable 10°–30°. Stage with higher durability and smoother surface “Alumite,” a new hardening treatment, has been applied to the stage surface to increase durability and smoothness. This facilitates the smooth exchange of specimens while preventing the surface from being scratched by the repeated exchange of slide glasses. — Battery-drive capability using optional lithium batteries means model can be used anywhere. Refocusing stage The stage can be lowered by pushing the lever down, and will return to its former height when the lever is pressed again. This feature eliminates the need to refocus the image manually each time the specimen is changed and the slide oiled, greatly improving microscopy productivity. — Bright 6V-30W halogen lamp as light source. — ND8 filter is built-in as standard. — Enables brightfield, darkfield, epifluorescence, phase contrast and simple polarizing observations. Eyepiece length is extendable up to 40mm. 4 Utilizing computer-aided engineering (CAE), Nikon has successfully increased the solidity of the microscope body and produced a sleek, modern design. Although compact in size, the new microscopes boast superb durability and stability, even during applications in which they are upgraded with various attachments. 4 5 MICR249 / Ergo-View enables quick magnification switching and marking . . . in harmony with your vision The compact Ergo-View cytodiagnostic unit has been developed for easier and more comfortable cytology examinations. Retrofittable to the 50i/55i, it can quickly move between 10X and 40X at the flip of a switch, utilizing a motorized mechanism. — Compact, sleek design. — Easily attachable to 50i/55i. — Fast and accurate motorized magnification changeover with hand switch. — Unique quiet, vibration-free mechanism for magnification change ensures superb parfocality of images and no deviation in focusing. — Easy marking while observing the specimen through eyepieces. — Quick exchange of slides with one hand is possible by using an optional specimen holder for one slide. CF1 60—tried and tested infinity optics Highly acclaimed optics combine the CF design with infinity optics and utilize a 60mm parfocal distance, resulting in longer working distances and high N.A.’s, while producing crisp, clear images with minimal flare. The CF160 optics are perfect for both observations and capturing images with a digital camera, and they provide a flexible upgrade path to accommodate various accessories to meet individual applications. Constant brightness when combined with the 55i When the Ergo-View is mounted on the 55i, which utilizes white LED illumination, light intensity is automatically adjusted in conjunction with changes in magnification. Constant brightness is provided throughout the inspection, preventing eyestrain. . . . in harmony with your hands DSC port enables digital imaging in comfort A new optional DSC port can be combined with an ergonomic tilting/telescoping tube to balance user needs for both digital-image capture and comfortable viewing. Including a 0.7X lens that is designed to optimize the image to the 2/3inch CCD, the same area as that viewed through the eyepieces can be captured with a C-mount digital camera. A centering and focus adjustment mechanism is also provided. Change between 10X and 40X at the flip of a switch. 10X 40X DS-5M-L1 “Digital Sight” standalone digital camera The standalone design of the DS-5M-L1 allows 5-million-pixel, high-definition images to be captured without connecting to a personal computer. The cameracontrol unit features a built-in 6.3-inch LCD monitor with excellent resolution, enabling focusing to be carried out on the screen. Optimal camera settings have been preprogrammed for each observation method and are selectable from a menu. Users can take advantage of the camera’s network functions to send or share images over a network and manage images on a server, saving time and increasing productivity. A 2-megapixel model, the DS-2Mv-L1, which features smooth live image movement and simple focusing, is also available. Marking is easily performed while observing the image. Quick-dry ink facilitates fast, clean marking. Wide variety of tools Scene menu 6 Count marking Distance measurement between 2 points 7 MICR249 / . . . in harmony with your needs . . . applying various methodologies Pathology tests Cytodiagnosis tests As the 55i model uses white LED illumination, it can maintain the same color temperature even if the brightness is changed. Observations extending to many hours can be carried out in a natural posture, without physical strain because an ergonomic tube enables the flexible adjustment of the eye point. The new Ergo-View cytodiagnostic unit makes magnification switching and marking faster and easier. When it is combined with the 55i model, changes in the amount of LED light are aligned with magnification changes, enabling observation at a constant brightness. . . . and a wide range of accessories New accessories complement a rich variety of existing accessories. Epi-fluorescence attachment Eye-level riser This dedicated turret-type epi-illuminator can accommodate four filter cubes despite its compact size. Filters or mirrors in the filter cubes can be easily replaced to create the desired combination. The attachment’s glowing display tags make it easy to recognize filter names in a darkened room. The eye-level riser can raise the eye-point height by 25mm at one time (up to 100mm* maximum). *: The number of risers that can be used at any one time depends on the tube or intermediate modules being used. Teaching head (50i) These teaching heads, which come with a built-in pointer, facilitate simultaneous viewing of the same specimen by up to 5 persons without compromising image brightness. DS-5M-L1 Digital Sight digital camera system 5-megapixel, high-definition images can be easily captured without connection to a PC or external monitor. The camera controller includes a built-in, 6.3-inch LCD monitor with extremely high resolution, enabling the operator to focus the image on the monitor. The “Scene Function” allows the optimum camera setting for each observation method to be selected from the menu. The DS-2Mv-L1, that delivers smooth live image movement, is also available. Ergo-View cytodiagnostic unit Epi-fluorescence microscopy Phase contrast microscopy (50i) A new dedicated turret-type epi-fluorescence illuminator has a quick-change mechanism combined with a unique filter-lock system and front-mounted shutter, providing the ultimate in clinical fluorescence diagnostic microscopy. Developed expressly for this technique, our unique Apodized Phase Contrast objectives enable the detection of minute structures— previously difficult to detect due to annoying halos—with excellent contrast and a much wider tonal range. This is ideal for urinary sediment tests. The Ergo-View, a compact cytodiagnostic unit, can be easily retrofitted to the microscope. Utilizing a motorized control, it enables easy switching between 10X and 40X magnifications at the flip of a switch. Marking operation is also easy, even while viewing the specimen. In addition to the ergonomic tube, which features a viewing angle of 10°–30° and eyepieces that can be extended up to 40mm, the following Eclipse series tubes are also available. Ergonomic tube Darkfield (50i) Darkfield microscopy is ideal for observing specimens such as blood and the minute structures of flagella. A dry- or oil-type darkfield condenser can be selected. Binocular tube B Trinocular tube F2UW Mounted between the main body and eyepiece tube, the double port enables operators to use two CCTV camera systems simultaneously or one CCTV camera and one digital camera. Trinocular tube T2UW The magnification module allows the intermediate magnification to be changed to 1X, 1.25X, 1.5X or 2X, enabling the operator to frame the image to be captured with a digital camera to match the view field seen through the eyepieces. Drawing tube DSC port The optional DSC port allows a 2/3-inch CCD C-mount digital camera to be mounted to the ergonomic tube. 1 8 The FX series features a direct-projection system with swing-out prism to ensure fast exposures and accurate metering. U-III: 0.1% and 1% spot exposure and 35% integrated-average measurement modes H-III: 1% spot and 35% integrated-average measurement modes P-III: Manual exposure model Lithium-ion battery (55i) The optional lithium-ion battery increases the mobility of the 55i by freeing it of dependence on an electrical outlet. Magnification module 2 Simple polarizing microscopy can be performed easily using dedicated accessories. Sensitive color-polarizing microscopy enables uric acid crystals forming inside an organism to be identified by changing the interference color via a lever. Such microscopy is ideal for gout tests. 2-person (side-by-side) version Double port Eyepiece tubes Simple polarizing/sensitive color-polarizing microscopy (50i) FX-III series photomicrographic equipment The observation image and drawing can be seen together through the eyepieces. When necessary, 100% of the light can be sent to the observation port. Simple polarizing set and sensitive color polarizing unit (50i) These easily installed options enable operators to carry out simple polarizing/sensitive color polarizing observations. Simple polarizing set Sensitive color polarizing unit 1 9 MICR249 System Diagram *: 50i model. Eyepieces Camera Mounts FX-III Series Photomicrographic System ENGmount TV Adapter 0.45x, 0.6x Projection Lens PLI 2x, PLI 2.5x, PLI 4x, PLI 5x C-mount Camera ENG-mount Camera ENG-mount Zooming Adapter ENG-mount TV Adapter Relay Lens 1X TV Zoom Lens C-mount TV Adapter A C-mount TV Adapter 0.35x, 0.45x, 0.6x, 0.7x Relay Lens 1X CFI 10x CFI 10x M C-CT Centering Telescope CFI UW 10x C-mount Adapter 0.55x Epi-fl Filter Cubes Built around an optimum digital-imaging platform, the 80i can visualize weakly fluorescing molecules with much higher brightness and contrast, while reducing background noise. CFI UW 10x M E F C-mount TV Adapter A C-0.7x DXM Relay Lens CFI 15x J-FL 50i/55i Epi-Fluorescence Attachment The new Plan Apo VC objectives and unique “fly-eye” optics guarantee uniform brightness over the whole view field and unparalleled resolution to the peripheries of the image. These remarkable achievements, which take digital imaging to new heights, are the result of Nikon’s breakthroughs in optical technologies and precision engineering. I TE-AT Double Lanphouse Adapter C-mount TV Adapter VM4x C-mount TV Adapter VM2.5x V-T Photo Adapter DS-2M Series DXM1200C, DS-5M Series, etc. C-mount TV Adapter C-mount Zooming Adapter CFI 12.5x I G H NI C-ISA Intermediate Tube* w/Simple Analyzer A Nosepieces Stages / Specimen Holders C-HL2 Specimen Holder (2 slides: Left) C-N Sextuple Nosepiece J-CY Cytodiagnostic Unit —Hi S/N Fluorescence System. The universal epi-fluorescence illuminator and digital-imaging head incorporate Nikon’s unique Hi S/N Fluorescence System, which employs a “Noise Terminator” to achieve unparalleled contrast with excellent S/N ratios. An optional “Excitation Balancer” allows specific wavelengths to be emphasized in multi-stained specimens. C-ER Eye-level Riser C-IA Intermediate Tube* w/Analyzer C-HC1 Specimen Holder (1 slide) Y-IDP Double Port (45-55 / 0-100, 0-100) Advanced research microscope CFI60 Objective Lens Cable Set for 55i & Cytodiagnostic Unit C-SR Mechanical Stage G Intermediate Modules B H Y-TV 0.55 TV Tube — New DIC System. A perfect balance of high resolution and high contrast is possible, even at low magnifications. Three types of DIC prisms are available: standard, high-contrast, and high-resolution. Y-IM Magnification Module C-HS Hand Switch Y-THP Pointer Unit Y-IDT Drawing Tube Y-THF Teaching Unit Face to Face* Y-QT Quadrocular Adapter Y-TV TV Tube Nikon offers various options to best suit your digital-imaging needs Y-THS Teaching Unit Side by Side B* H Y-THA AC Adapter Eyepiece Tubes DXM1200C, DS-5M Series, etc. E F F Y-TB Binocular Tube B E F Y-TF2 Trinocular Tube F2UW Y-THM Main Teaching Unit* F C-TE Ergonomic Binocular Tube Y-TT2 Trinocular Tube T2UW C-TEP DSC Port Y-THPS Support for Side by Side Hg Lamphouse HMX-3B EN-EL1 Li-ion Battery A B A DS AC Adapter C-SHG1 Power Supply for HG100W D F C-FC Epi-Fl Collector Lens C-TP* Polarizer with First-order Red Tint Plate Xe Lamphouse HMX-4 Xe Lamp Socket 75W Xenon Power Supply 75W Quartz Epi-FI Collector Lens C D C-FC Epi-Fl Collector Lens Darkfield* Condenser (dry) C-CEL Expander Lens 10 C-C Achromat /Aplanat Condenser C-C Achromat Condenser Swing-out Condenser 2-100x C-C Abbe Condenser LWD Achromat Condenser — Motorized operation enables brightfield observations through the monitor’s GUI. C-C Slide Achromat Condenser 2-100X C-C Achromat Swing-out Condenser 1-100x C-C* Phase Contrast Condenser C-C Low Power Condenser — Instant recall of previous observations can be achieved by clicking the numbered button. — Remote operation* and viewing of images is possible through networked computers anywhere. * Some operations are limited I Polarizers Condensers The COOLSCOPE is revolutionary in every respect, from its looks to its functionality and ease of use. After loading the slide glass preparation onto the tray, users need only click the mouse to operate the microscope. Observations, image recording and networking can all be done with a single unit of the COOLSCOPE— no need for a PC. The popular image of a microscope has undergone a major metamorphosis. — The point of interest can be easily moved to the center by simply clicking the point in the micro image. In the macro image, the selected point is enlarged and displayed in the micro image. F C-SP* Simple Polarizer Digital microscope — Simultaneous display of both enlargements (micro) of specimen portions and whole image (macro) on a single screen. Hg Lamphouse HMX-4B C D Mercury Lamp Socket S 100W C-FC Epi-Fl Collector Lens B C An all-in-one microscope that can be operated by mouse Y-THR Teaching Unit Side by Side A Lamphouses Darkfield* Condenser (oil) — Digital-Imaging Head. This creates an optimum digital-imaging system that enables fluorescence imaging with outstanding results. When the DS-5M-L1 digital camera is mounted to this head, observation data such as magnification and fluorescence filters in use is automatically detected and can be saved together with the image file. HMX Lamphouse Halogen Lamp Socket 100W/HMX UN2 Transformer 100W Please visit our dedicated website, where you will find extensive information on the uses and operation of the COOLSCOPE. www.coolscope.com *1: Use the dedicated 0.45X adapter for the double port sub-port. 11 MICR249 Specifications Main body 50i Magnification 10 –1500X 55i Optical system CFI60 Infinity Optical System Coarse/fine focusing Fine: 0.1mm per rotation, Coarse: 13.8mm per rotation, Minimum reading: 1 micron Coarse motion torque adjustable, Refocusing function (with stopper) Illumination 6V-30W halogen lamp 100-240V (worldwide voltage) White LED array AC adapter (100-240V) or optional lithium battery (7.4V) Built-in filter ND8 LA60 color balance filter Eyepiece tube Binocular tube B (for F.O.V. 22mm) Trinocular tube F2UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 0/100) Trinocular tube T2UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 20/80, 0/100) Ergonomic binocular tube (for F.O.V. 22mm, inclination:10-30°, extension: 40mm); DSC port: 100/0, 50/50 (optional) Eyepiece lens 10X (F.O.V.: 22mm), 10X M photo mask (F.O.V.: 25mm), 12.5X (F.O.V.: 16mm), 15X (F.O.V.: 14.5mm), UW 10X (F.O.V.: 25mm), UW 10X M photo mask (F.O.V.: 25mm) Nosepiece Sextuple nosepiece Ergo-View cytodiagnostic unit (Motorized changeover 10X to 40X with hand switch, Stamp type marking Stage Super-hard Alumite coated surface, Stay-in-position stage handle, Stage handle height and tension adjustable Rectangular 159mm X 243mm surface stage, 78mm X 54mm cross travel (x-y movement) 1-slide or 2-slide specimen holder available (option) — Automatic illumination adjustment with Ergo-View Condenser focusing stroke 27mm Intermediate accessories Epi-fluorescence illuminator (4 filter positions), Magnification module, Eye-level riser, Double port Teaching head Observation method — Brightfield, Epi-fluorescence Darkfield, Phase contrast, Simple polarizing — Dimensional Diagrams 60 200 200 330 404 330 60 486 Unit: mm I FIL FIL O 355 208 355 208 Configured with binocular tube B Configured with ergonomic tube Nikon has reduced the amount of chromium, cadmium and lead used in the Eclipse-i Series to an absolute minimum to diminish its environmental impact. Microscopy images courtesy of : 1 Nihon University Itabashi Hospital 2 Naoyuki Miyokawa, M.D., Ph.D., Associate Professor, Dept. of Surgical Pathology, Asahikawa Medical College Hospital. Please contact Nikon for a handy pamphlet listing compatible accessories, including objectives and epi-fluorescence filters. Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. February 2006 ©2003-6 NIKON CORPORATION WARNING TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT. * Monitor images are simulated. Company names and product names appearing in this brochure are their registered trademarks or trademarks. NIKON INSTECH CO., LTD. NIKON INSTRUMENTS EUROPE B.V. NIKON INSTRUMENTS INC. Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0005, Japan phone: +81-44-223-2167 fax: +81-44-223-2182 www.nikon-instruments.jp/eng/ P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands phone: +31-20-44-96-222 fax: +31-20-44-96-298 www.nikon-instruments.com/ 1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A. phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306 www.nikonusa.com/ NIKON INSTRUMENTS (SHANGHAI) CO., LTD. CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030 (Beijing office) phone: +86-10-5869-2255 fax: +86-10-5869-2277 (Guangzhou office) phone: +86-20-3882-0552 fax: +86-20-3882-0580 NIKON SINGAPORE PTE LTD SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668 NIKON MALAYSIA SDN. BHD. MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387 NIKON INSTRUMENTS KOREA CO., LTD. KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415 NIKON CANADA INC. NIKON FRANCE S.A.S. CANADA phone: +1-905-625-9910 fax: +1-905-625-0103 FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33 NIKON GMBH GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322 NIKON INSTRUMENTS S.p.A. ITALY phone: + 39-55-3009601 fax: + 39-55-300993 NIKON AG SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861 NIKON UK LTD. UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584 NIKON CORPORATION Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100-8331, Japan www.nikon.com/ Printed in Japan (0602-06)T Code No. 2CE-MSHH-5 En This brochure is printed on recycled paper made from 40% used material. MICR249 ECLIPSE 5Oi/55i Accessories Accessories Clinical & Laboratory Microscope Accessories / ECLIPSE i-Series Website www.nikon-i.com MICR249 Cytodiagnosis Tests Cytodiagnostic Accessories This compact Ergo-View cytodiagnostic unit can be easily retrofitted to the 50i or 55i microscope, to facilitate easier and more comfortable cytology examinations. •Motorized magnification switchover between 10X and 40X at the flip of a switch. •Rapid and quiet magnification changeover with no vibration. This eliminates eye strain due to image blur. •Excellent image parfocality minimizes focus deviation, eliminating the need for focus adjustment each time the magnification is changed. •In configuration with the ECLIPSE 55i that adopts LED illumination, light intensity is automatically adjusted following the magnification change. This ensures comfortable observations with constant brightness. •Easy object marking while observing the specimen through eyepieces. •Marking ink is a cartridge type and can be easily exchanged. ■ Specific Accessories for ECLIPSE 50i 1 J-CY Cytodiagnostic Unit 2 2 DS AC Adapter 3 C-HS Hand Switch 1 3 ■ Specific Accessories for ECLIPSE 55i 1 J-CY Cytodiagnostic Unit 1 2 Cable Set for Cytodiagnostic Unit 2 3 C-HS Hand Switch 3 Phase Contrast Phase Contrast Accessories (ECLIPSE 50i) These accessories produce well-defined high contrast images with neutral background coloration regardless of the magnification range. The CFI Plan Fluor DLL objectives can be universally used for phase contrast, epi-fluorescence, brightfield, as well as DIC applications. The CFI Achromat ADL objectives clearly visualize the minute structure of thick phase objects by minimizing halos. ■ Specific Accessories 1 3 1 C-C Phase Contrast Condenser 2 CFI Plan Fluor DLL Objectives 3 Filter, 45mm GIF 4 2 2 4 C-CT Centering Telescope MICR249 Darkfield Darkfield Accessories (ECLIPSE 50i) These darkfield accessories allow clear observation of blood or the minute structure of flagella. Dry- and oil-type condensers are available. The expander lens is used to obtain illumination with greater brightness. It is also possible to perform darkfield microscopy using a phase contrast condenser. ■ Specific Accessories 1 C-CEL Expander Lens 2 C-C Darkfield Condenser (Dry) 3 C-C Darkfield Condenser (Oil) 1 2 3 Simple Polarizing Simple Polarizing Accessories ( ECLIPSE 50i ) Ideal for observing birefringent samples such as collagen, amyloids and crystals. Operation is as simple as inserting a polarizer over the field lens and an analyzer in the arm slot. The field number has been increased to 25. ■ Specific Accessories 1 C-SP Simple Polarizer 1 2 C-ISA Intermediate Tube w/Simple Analyzer 2 Sensitive Color Polarizing Epi-fluorescence Sensitive Color Polarizing Accessories ( ECLIPSE 50i ) Enables easy gout and pseudo-gout tests. ■ Specific Accessories 1 1 C-TP Polarizer with First-order Red Tint Plate 2 C-IA Intermediate Tube w/Analyzer 2 Epi-fluorescence Accessories The epi-fl illuminator is a compact and easy-to-use turret-type exclusively developed for the ECLIPSE 50i and 55i. ■ Specific Accessories •Four filter cubes can be accommodated, and they are easily exchanged. •Filters or mirror in the filter cubes can be easily replaced to create the desired combination. •Filter position labels glow in the dark for easy identification. 1 J-FL Epi-fluorescence Attachment 2 Filter Position Labels 4 3 Light Shielding Plate 4 Hg Lamphouse HMX-4B 1 5 C-FC Epi-fl Collector Lens 6 Light Shielding Tube 6 5 2 7 C-FC Centering Tool 8 8 C-SHG1 Power Supply for HG 100W 7 9 C-FL Epi-fl Filter Cubes 9 ! CFI Plan Fluor Objectives 3 ! 3 MICR249 Epi-fluorescence Filters Fluorescence Filter Cubes Filters UV-2A •Standard filter block for UV •For DAPI, cutting off FITC (green) and TRITC (red) •Soft-coated type for high signal/noise •Band-Pass Barrier Filter used to cut off green and red UV-1A EX 365/10 DM 400 BA 400 •Narrow band pass – only 365nm (i line) of Mercury spectrum used •Narrow band pass minimizes auto-fluorescence and photo-bleaching UV-2B EX 330-380 DM 400 BA 435 •Darker background than UV-2A V-2A EX 380-420 DM 430 BA 450 •Standard filter block for V BV-2A EX 400-440 DM 455 BA 470 •Standard filter block for BV BV-1A EX 435/10 EM 455 BA 470 •Narrow band pass – only 435nm (g line) of Mercury spectrum used •Narrow band pass minimizes auto-fluorescence and photo-bleaching B-3A EX 420-490 DM 505 BA 520 •Wide band pass – recommended for halogen illumination only B-2A EX 450-490 DM 505 BA 520 •Standard filter block for B •For FITC + Counter-stain (TRITC, PI) B-2E/C (FITC) EX 465-495 DM 505 BA 515-555 •Soft coated type for high signal/noise •For FITC (green), cutting off Rhodamine red •Band-pass Barrier Filter used to cut off red B-1A EX 470-490 DM 505 BA 520 •Narrower excitation range than B-2A •FITC+Counter-stain (TRITC, PI) B-1E EX 470-490 DM 505 BA 520-560 •For FITC (green), cutting off Rhodamine red •Band-Pass Barrier Filter used to cut off red G-2A EX 510-560 DM 575 BA 590 •Standard filter block for G G-2E/C (TRITC) EX 540/25 DM 565 BA 605/55 •For TRITC (Rhodamine) •Soft coated type for high signal/noise •Band-Pass Barrier Filter used to cut off reds above 643nm G-1B EX 546/10 DM 575 BA 590 •Narrow band pass – only 546nm (e line) of Mercury spectrum used •Narrow band pass minimizes auto-fluorescence and photo-bleaching G-2B EX 510-560 DM 575 BA 610 •610nm barrier provides darker background and deep red emission Y-2E/C (Texas Red) EX 540-580 DM 595 BA 600-660 •For Texas Red® •Soft coated type for high signal/noise •Band-Pass Barrier Filter used to cut off reds above 660nm G Y Fluorescence Reagents and Dedicated Filters*1 for Fluorescence Protein Wavelengths Filters/Reagents GFP-L GFP-B BFP CFP YFP Cy 3 Cy 5 Cy 7 4 Applications EX 340-380 DM 400 BA 435-485 BV B Characteristics UV-2E/C (DAPI) UV V Wavelengths EX 330-380 DM 400 BA 420 EX480/40, DM505, BA510 EX480/40, DM505, BA535/50 EX380/30, DM420, BA460/50 EX436/20, DM455, BA480/40 EX500/20, DM515, BA520 EX535/50, DM565, BA610/75 EX620/60, DM660, BA700/75 EX710/75, DM750, BA810/90 •DAPI •Hoechst 33258/33342 •AMCA •Cascade Blue® •Autofluorescence •Catecholamine •Serotonin •Tetracycline •Quinacrine •Quinacrine Mustard (QM) •Thioflavine S •Acriflavine •FITC •Acridine Orange •Auramine O •Coriphosphine O •Bodipy® •Fluo-3 •DIO •TRITC •Rhodamine B200 •Propidium iodide •R-Phycoerythrin •B-Phycoerythrin •Dil •Ethidium Bromide •Texas Red® Multi-Band Filters*1 Filters Abbreviations Reagents F-R FITC/Rhodamine Dual Triple F-T FITC/Texas Red D-F DAPI/FITC D-F-R DAPI/FITC/Rhodamine D-F-T DAPI/FITC/Texas Red Filters are consumables. Filters marked with an asterisk (*1), in particular, come with special coatings, so these should be replaced in the shortest possible period of time. Other filter combinations are available. Please ask your local Nikon distributor for a complete list of filters or inquire about special custom filter combinations possible. MICR249 CFI Objectives CFI Objectives Brightfield (CFI) 0.10 30.00 - 4× 0.13 17.10 - 0.25 7.00 - 10× 0.30 16.00 0.17 LWD 20× 0.40 3.90 0.17 20× 0.50 2.10 0.17 40× 0.65 0.65 0.17 0.75 0.35 0.17 LWD 40×C 0.55 2.7-1.7 0-2.0 20×Mi (Multi-immersion) 60× 0.80 0.30 0.17 40× 0.75 0.72 0.17 1.30 0.20 0.17 0.85 0.30 0.11-0.23 0.5-1.25 0.22 0.17 0.90 0.30 0.14-0.20 1.25 0.23 0.23 0.17 0.25 7.00 - Achromat 0.40 3.90 0.17 0.40 3.10 1.2 LWD DL 40×C 0.65 0.55 1.25 0.25 0.65 2.7-1.7 0.23 6.10 6.20 1.2 1.2 LWD ADL 40×C 0.55 2.7-1.7 0-2.0 P 4× 0.10 30.00 - 0.25 7.00 - LWD P 20× 0.40 3.90 0.17 P 40× 0.65 0.65 0.17 P 100×H 1.25 0.23 0.17 0.04 3.20 - UW 2× 0.06 7.50 - 4× 0.10 30.00 - Brightfield 10× 0.25 10.50 - (CFI Plan) 20× Plan Achromat 0.56 0.17 0.17 50×H 0.90 0.35 - 100×H 1.25 0.20 0.17 No Cover NCG 40× 0.65 0.48 0 (CFI Plan) NCG 100× 0.90 0.26 0 (CFI Plan) 0.17 0.2 0.17 DL 4× 0.13 16.40 1.2 DL 10× 0.30 15.20 1.2 DLL 10× 0.30 16.00 - (CFI Plan Fluor) DLL 20× DLL 40× 0.50 2.10 0.17 Fluor Super Fluor 2.00 0 Epi-fl 20×W 0.50 2.00 0 (CFI Fluor) 40×W 0.80 2.00 0 60×W 1.00 2.00 0 4× 0.20 15.5 - 10× 0.50 1.20 0.17 20× 0.75 1.00 0.17 (CFI S Fluor) 40× 0.90 0.30 0.11-0.23 1.30 0.22 0.17 0.5-1.3 0.20 0.17 40×H 100×H( iris) - 10× 0.45 4.00 0.17 20× 0.75 1.00 0.17 40× 0.95 0.14 0.11-0.23 0.17 Evanescent 60×H 1.40 0.21 0.17 VC 60×H 1.40 0.13 0.17 VC 60×WI 1.20 0.27 0.15-0.18 VC 100×H 1.40 0.13 0.17 NCG 100×H 1.40 0.17 0 TIRF 60×H 1.45 0.13 0.10-0.22 1.45 0.13 0.17 DM 20× 0.75 1.00 0.17 DM 40× 0.95 0.14 0.11-0.23 DM 40×H 1.00 0.16 0.17 DM 60× 0.95 0.15 0.11-0.23 DM 60×H 1.40 0.13 0.17 DM 100×H 1.40 0.13 0.17 TIRF (CFI Plan Apo) TIRF 100×H (CFI Plan Apo) 0.30 15.70 0.11-0.23 Phase Contrast 0.17 0.20 0.15 0.17 - 4× 0.16 0.17 0.20 8.50 1.00 1.20 0.56 0.10 0.95 10.50 1.25 0.17 2× 60× 0.40 0.65 0.17 0.20 40×H 0.25 DL 100×H 0.72 1.30 Brightfield DL 20× DL 40× 0.75 DLL 100×H (CFI Plan Apo) DL 10× 10×W Epi-fl Plan Apochromat UW 1× 0.65 0.2 1.2 P 10× 40× 1.30 0.5-1.3 Phase Contrast - 3.10 1.20 100×H 100×H ( iris ) 0.17 0.25 0.40 100× 0-2.0 0.40 2.10 60×SH ( iris ) 0.17 ADL 10× New Phase LWD ADL 20×F Contrast LWD ADL 40×F (CFI) 0.55 60× Plan Fluor 0.5-1.25 LWD DL 20×F DL 40× Epi-fl (CFI Plan Fluor) 40×H 0.17 LWD DL 20× BM 10×A Phase Contrast W.D. Cover-glass （mm） Thickness 10× DL 100×H (CFI) N.A. 4× DL 10× Polarizing Name N.A. 100×H( iris) (CFI) Use Name 100×H Phase Contrast W.D. Cover-glass （mm） Thickness Type Type Use 5 MICR249 Accessories Others Eyepiece Tubes Name Beam Split Ratio Field Number Binocular Tube B Observation 100% 22 ISO Photo Tube Trinocular Tube FUW Observation 100% / Photo 100% 22/25 Attachable Trinocular Tube TUW Observation 100% / Photo 100% / 22/25 Observation 20%, Photo 80% Attachable Ergonomic Binocular Tube Observation 100% / Observation 50%, Photo 50% (with DSC port) 22 Eyepiece Lenses Name (Magnification) Field Number CFI 10× 22 CFI 10×M 22 CFI 12.5X 16 CFI 15× 14.5 CFI UW 10× 25 CFI UW 10×M 25 Reticule Photomask Photomask Condensers Type N.A. W.D. (mm) Magnifications Abbe Condenser 0.9 1.9 4-100× Achromat Condenser 0.8 4.2 4-100× Achromat Swing-out Condenser 2-100X 0.9/0.22 1.8 2-100× Achromat Swing-out Condenser 1-100X 0.8/0.12 3.2 1-100× Achromat Aplanat Condenser 1.40 1.6 10-100× LWD Achromat Condenser 0.65 10.2 4-40× Low Power Condenser 0.13 10.2 1-4× Darkfield Condenser (Dry)* 0.8-0.95 4 20-40× Darkfield Condenser (Oil)* 1.2-1.43 1.5 20-100× Phase Contrast Condenser* 0.9 1.9 10-100× *For ECLIPSE 50i CCTV Adapters 2.5X, 4X relay lenses are ideal for video enhanced contrast (VEC applications. Use 0.35X, 0.45X and 0.7X relay lenses for 1/3-, 1/2-, 2/3-inch CCD cameras, respectively. 0.9 to 2.2X zoom relay lenses are also available. 6 MICR249 Accessories Teaching Heads ( ECLIPSE 50i ) Teaching heads facilitate simultaneous viewing of the same specimen without compromising image brightness. A pointer to show the point of interest is provided. Available from 2-person (side-by-side or face-to-face) to 5-person types. 2-person (face-to-face) version 2-person (side-by-side) version Eye-level Riser Drawing Tube Can raise the eye-point height in 25mm increments, up to 100mm maximum depending on the configuration. The drawing tube allows both the image of a specimen and the drawing to be seen through the eyepieces. A dedicated adapter is necessary to attach it. FX-III Series Photomicrographic Equipment Double Port The FX-III series utilizes a directprojection system with a swing-out prism for fast exposure setting and accurate metering. U-III: 0.1% and 1% spot exposure, and 35% integrated-average measurement modes H-III: 1% spot and 35% integratedaverage measurement modes P-III: Manual exposure model The double port enables the use of one CCTV camera and one digital camera, or two digital cameras simultaneously. H-III Magnification Module The turret system allows the intermediate magnification to be changed to 1X, 1.25X, 1.5X or 2X. Specimen Holders Two types of 2-slide holders (opening to the right or left) and a single-slide holder enabling the quick exchange of the specimen with one hand are available. 7 MICR249 Specifications Main body 50i 55i Magnification 10 –1500X Optical system CFI60 Infinity Optical System Coarse/fine focusing Fine: 0.1mm per rotation, Coarse: 13.8mm per rotation, Minimum reading: 1 micron Coarse motion torque adjustable, Refocusing function (with stopper) Illumination 6V-30W halogen lamp 100-240V (worldwide voltage) White LED array AC adapter (100-240V) or optional lithium battery (7.4V) Built-in filter ND8 LA60 color balance filter Eyepiece tube Binocular tube B (for F.O.V. 22mm) Trinocular tube "F" UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 0/100) Trinocular tube "T" UW (for F.O.V. 22mm/25mm, observation/photo: 100/0, 20/80, 0/100) Ergonomic binocular tube (for F.O.V. 22mm, inclination:10-30°, extension: 40mm); DSC port: 100/0, 50/50 (optional) Eyepiece lens 10X (F.O.V.: 22mm), 10X M photo mask (F.O.V.: 25mm), 12.5X (F.O.V.: 16mm), 15X (F.O.V.: 14.5mm), UW 10X (F.O.V.: 25mm), UW 10X M photo mask (F.O.V.: 25mm) Nosepiece Sextuple nosepiece Ergo-View cytodiagnostic unit (Motorized changeover 10X to 40X with hand switch, Stamp type marking — Automatic illumination adjustment with Ergo-View Stage Super-hard Alumite coated surface, Stay-in-position stage handle, Stage handle height and tension adjustable Rectangular 159mm X 243mm surface stage, 78mm X 54mm cross travel (x-y movement) 1-slide or 2-slide specimen holder available (option) Condenser focusing stroke 27mm Intermediate accessories Epi-fluorescence illuminator (4 filter positions), Magnification module, Eye-level riser, Double port Teaching head Observation method — Brightfield, Epi-fluorescence Darkfield, Phase contrast, Simple polarizing — Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. June 2004. ©2003-4 NIKON CORPORATION WARNING TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT. Company names and product names appearing in this brochure are their registered trademarks or trademarks. NIKON INSTECH CO., LTD. NIKON INSTRUMENTS EUROPE B.V. NIKON INSTRUMENTS INC. Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0005, Japan phone: +81-44-223-2167 fax: +81-44-223-2182 www.nikon-instruments.jp/eng/ P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands phone: +31-20-44-96-222 fax: +31-20-44-96-298 www.nikon-instruments.com/ 1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A. phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306 www.nikonusa.com/ NIKON FRANCE S.A. FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33 NIKON GMBH GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322 NIKON INSTRUMENTS S.p.A. ITALY phone: + 39-55-3009601 fax: + 39-55-300993 NIKON AG SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861 NIKON UK LTD. UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584 NIKON CANADA INC. CANADA phone: +1-905-625-9910 fax: +1-905-625-0103 NIKON INSTRUMENTS (SHANGHAI) CO., LTD. CHINA phone: +86-21-5058-5055 fax: +86-21-5058-5060 NIKON SINGAPORE PTE LTD SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668 NIKON MALAYSIA SDN. BHD. MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387 NIKON CORPORATION Fuji Bldg., 2-3, Marunouchi 3-chome, Chiyoda-ku, Tokyo 100-8331, Japan www.nikon.com/ Printed in Japan (0406-06)T Code No. 2CE-MSJH-2 This brochure is printed on recycled paper made from 40% used material. En 8 MICR249 Specifications and Standards 8.1 Specifications Nikon Microscope ECLIPSE 50i Model ECLIPSE 50i Optical system Infinity-corrected CF optical system Focus up/down motion Objective: CF160 Objectives: Field number 22 (with ergonomic tube/binocular tube/cytodiagnostic unit), 25 (with trinocular eyepiece tube T/F) Nosepiece: Sextuple Drive system: Manual coarse/fine motion (calibration markings for fine motion: 1 µm/marking) Stroke: 2 mm upward, 28 mm downward With one-touch refocusing mechanism Lamp ratings 6V/30W halogen lamp Lamp type Halogen lamp (PHILIPS 5761) Average lamp life 100 hours Input ratings 100-240V AC; 230V (10%, 50/60Hz, 0.9A; 0.5A) Power cord • 100-120V areas: UL-approved detachable power cord set (3 conductor grounding Type SVT, AWG 18, 3 m long maximum, rated at 125V AC minimum) • 220-240V areas: EU/EN-approved 3-conductor power cord set (3 conductor grounding Type H05VV-F, 3 m long maximum, rated at 250V AC minimum) Operating conditions Temperature: 0 to 40°C Humidity: 85% RH max. (no condensation) Altitude: 2000 m max. Degree of pollution: Degree 2 Installation: Category II Electric shock protection class: Class I Indoor use only Transport/storage conditions Temperature: -20 to 60°C Humidity: 90% RH max. (no condensation) External dimensions and weight (main unit) External dimensions: 184 (W) x 358 (H) x 383 (D) mm (excluding projections) Weight: Approx. 9 kg 78