Microscope Components Guide

Transcript

Microscope Components Guide Choosing the Ideal UIS2 Optics Components for Your Equipment • OLYMPUS CORPORATION is ISO9001/ISO14001 certified. • OLYMPUS CORPORATION is FM553994/ISO9001 certified. • Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. • Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer. Printed in Japan M1703E-0310B Contents Welcome to UIS2 Optics --------------------------------------------------------- 3 — 4 Eyepieces/Filar Micrometer Eyepiece ------------------------------------------- 35 System Diagram ------------------------------------------------------------------- 5 — 6 Widefield Eyepieces UIS2 Objective Lenses ---------------------------------------------------------- 7 — 19 Super Widefield Eyepieces M Plan Apochromat MPLAPON Series ------------------------------8 M Plan Apochromat MPLAPON 100xO ------------------------------8 M Plan SemiApochromat MPLFLN Series ------------------------------- 9 Long WD M Plan SemiApochromat LMPLFLN Series ---------------------------- 10 M Plan Achromat MPLN Series --------------------------------- 11 LCD Long WD M Plan SemiApochromat LCPLFLN-LCD Series -------------------------12 Super Long WD M Plan Achromat SLMPLN Series -------------------------------13 IR Long WD M Plan Achromat LMPLN-IR Series -----------------------------14 IR M Plan Achromat LCPLN-IR Series ------------------------------15 M Plan SemiApochromat BD MPLFLN-BD Series ---------------------------16 M Plan SemiApochromat BDP MPLFLN-BDP Series -------------------------17 Long WD M Plan SemiApochromat BD LMPLFLN-BD Series -------------------------18 M Plan Achromat BD MPLN-BD Series -----------------------------19 Filar Micrometer Eyepiece Revolving Nosepieces --------------------------------------------------------- 36 — 37 Quintuple Revolving Nosepiece U-5RE-2-------------------------------------- 36 Sextuple Revolving Nosepiece with Slider Slot for DIC U-D6RE--------------------------------------- 36 Sextuple Revolving Nosepiece with Slider Slot for DIC with ESD Treatment U-D6RE-ESD --------------------------------- 36 Septuple Revolving Nosepiece with Slider Slot for DIC U-D7RE -------------------------------------- 36 Centerable Quadruple Revolving Nosepiece with Slider Slot for DIC U-P4RE -------------------------------------- 36 Centerable Sextuple Revolving Nosepiece with Slider Slot for DIC U-P6RE -------------------------------------- 36 Quintuple Revolving Nosepiece for BF/DF U-5BDRE ------------------------------------ 37 Quintuple Revolving Nosepiece for BF/DF with Slider Slot for DIC U-D5BDRE ----------------------------------- 37 Sextuple Revolving Nosepiece for BF/DF with Slider Slot for DIC U-D6BDRE ----------------------------------- 37 Centerable Quintuple Revolving Nosepiece U-P5BDRE ----------------------------------- 37 Adapter to Mount BF Objectives BD-M-AD ------------------------------------ 37 Microscope System BXFM --------------------------------------------------- 20 — 23 BXFM Frame BXFM BXFM-S Universal Stand Type 2 Compact Stand Large Stand BXFM-F -------------------------------------- 20 BXFM-F + BXFM-ILH + BXFM-ILHSPU ------- 21 BXFM-F + BXFM-ILHS ----------------------- 22 SZ2-STU2 ----------------------------------- 23 U-ST ----------------------------------------- 23 SZ-STL -------------------------------------- 23 Illumination Units --------------------------------------------------------------- 24 — 26 Video Camera Adapters ------------------------------------------------------- 38 — 39 Reflected Light Illuminator for BF/DF BX-RLA2 ------------------------------------- 24 Universal Reflected Light Illuminator BX-URA2 ------------------------------------ 24 Reflected Light Illuminators for BF BX-KMA/BX-KMA-ESD ----------------------- 25 Reflected Light Illuminator for BF U-KMAS ------------------------------------- 26 The wide range of Olympus components introduced here allows users in such diverse fields as research, inspection and production to take advantage of the quality, flexibility and outstanding optical performance of the UIS2 Optical System. C-mount Video Camera Ports Video Camera Mount Adapters Video Camera Port quite simply, the right choice for your equipment. Motorized Units ----------------------------------------------------------------- 40 — 44 75 W Xenon Apo Lamp Housing U-LH75XEAPO ------------------------------- 27 100 W Mercury Apo Lamp Housing U-LH100HGAPO ----------------------------- 27 100 W Mercury Lamp Housing U-LH100HG ---------------------------------- 27 Power Supply Unit for Xenon Lamp U-RX-T --------------------------------------- 27 Power Supply Unit for Mercury Lamp U-RFL-T ------------------------------------- 27 100 W Halogen Lamp Housings U-LH100-3/U-LH100IR/U-LH100L-3 -------- 28 External Power Supply TH4-100/200 -------------------------------- 28 Hand switch TH4-HS -------------------------------------- 28 Extension cord U-RMT --------------------------------------- 28 DF converter for BX-URA2 U-RCV --------------------------------------- 29 Fiber Adapter for Reflected Light Observation U-LGAD -------------------------------------- 29 Transmitted Light Guide Adapter SZX-TLGAD ---------------------------------- 29 Light Source LG-PS2 -------------------------------------- 29 Light Guide LG-SF ---------------------------------------- 29 Double Lamp House Adapter U-DULHA ------------------------------------ 30 Motorized BF/DF Reflected Light Illuminator+Motorized Nomarski DIC Sextuple Revolving Nosepiece+100 W Halogen Lamp Housing BX-RLAA + U-D6REMC + U-LH100-3 ------- 40 Motorized Universal Reflected Light Illuminator BX-RFAA ------------------------------------- 40 Motorized Sextuple BD Revolving Nosepiece with Slider Slot for DIC U-D6BDREMC ------------------------------- 41 Motorized Centerable Quintuple Revolving Nosepiece with Slider Slot for DIC U-P5BDREMC -------------------------------- 41 Motorized Quintuple BD Revolving Nosepiece with Slider Slot for DIC U-D5BDREMC ------------------------------- 41 Motorized Sextuple Revolving Nosepiece with Slider Slot for DIC U-D6REMC ---------------------------------- 41 Motorized Centerable Quintuple Revolving Nosepiece with Slider Slot for DIC U-P5REMC ----------------------------------- 41 Control Unit BX-UCB -------------------------------------- 42 Hand Switch U-HSTR2 ------------------------------------ 42 Control Box for Motorized Nosepiece and BF/DF Illuminator BX-REMCB ---------------------------------- 42 AC Adapter for BX-REMCB U-ACAD4515 -------------------------------- 42 Active Auto Focus Unit U-AFA2M-VIS -------------------------------- 43 Motorized Reflected Filter Wheel U-FWR --------------------------------------- 43 Motorized Illumination with Power Focus BXFMA-F ------------------------------------ 44 Focus Adjustment Knob Unit U-FH ----------------------------------------- 44 Focus Adjustment Knob Interface U-IFFH --------------------------------------- 44 Widefield Trinocular Tube U-TR30-2 ------------------------------------ 31 Widefield Trinocular Tube for IR U-TR30IR ------------------------------------ 31 Widefield Erect Image Trinocular Tube U-ETR-4 ------------------------------------- 31 Single Port Tube with Lens U-TLU ---------------------------------------- 31 Single Port Tube with Lens for IR U-TLUIR ------------------------------------- 31 Super Widefield Trinocular Tube U-SWTR-3 ----------------------------------- 32 Super Widefield Erect Image Trinocular Tube U-SWETR ------------------------------------ 32 Super Widefield Erect Image Tilting Trinocular Tube MX-SWETTR --------------------------------- 32 Deep Ultraviolet Observation System -------------------------------------------- 45 UV248 Compatible Intermediate Tube U-UVF248IM --------------------------------- 45 UV Quartz Light Guide U-UVF2FV/5FB ------------------------------- 45 UV248 Compatible Light Source Box + Mercury Xenon Lamp Housing U-UVF248LB + U-LH80HBXE ---------------- 45 Intermediate Tubes & Accessories ---------------------------------------- 33 — 34 Optical Terminology ------------------------------------------------------------ 46 — 50 Magnification Changer U-CA ----------------------------------------- 33 Magnification Changer 2x U-ECA --------------------------------------- 33 Trinocular Intermediate Attachment U-TRU --------------------------------------- 33 Dual Port U-DP ----------------------------------------- 34 Dual Port 1x U-DP1xC ------------------------------------- 34 Eyepoint Adjuster U-EPA2 -------------------------------------- 34 Arrow Pointer U-APT --------------------------------------- 34 1 U-TV0.25xC, U-TV0.35xC-2, U-TV0.5xC-3, U-TV0.63xC ------------------- 38 U-CMAD3, U-BMAD, U-SMAD, U-TMAD, U-FMT ----------------------------- 39 U-TV1x-2 ------------------------------------ 39 Light Source Units -------------------------------------------------------------- 27 — 30 Observation Tubes ------------------------------------------------------------- 31 — 32 That's why installing Olympus microscope components is, WHN10x, WHN10x-H, CROSSWHN10x, WH15x --------------------- 35 SWH10x-H, MICROSWH10x, CROSSSWH10x ------------------------------ 35 U-OSM --------------------------------------- 35 2 Welcome to UIS2 Optics Welcome to UIS2 Optics UIS2: The System That Maximizes the Advantage of Infinity-corrected Optics What is Infinity-corrected Optics? light between the objective lens and tube lens, allowing the creation of user-specific or taskspecific optical systems. To establish real flexibility with such a system, it is necessary to eliminate the occurrence of coma aberration. UIS2 optics is an infinity-corrected optical system — in other words, a system in which light passes from the specimen through the objective lens without forming an image along the way. Instead, it travels in the form of infinity parallel rays to the tube lens. The tube lens is where the intermediate image is formed, whereas in finite-corrected optics, this is done by the objective lens. *In UIS2 objective lenses, the parfocal distance is designed at Figure 2 Advantages of Infinity-corrected Optical System Finite-corrected Optical System Figure 1 Infinity-corrected and Finite-corrected Optical System Principles Infinity-corrected Optical System UIS/UIS2 Objective Lens Tube Lens Finite-corrected Optical System Objective Lens 4. Lightening Weight has been reduced to approximately 2/3 that of conventional products by using an aluminum objective lens barrel cover. This has the effect of lightening the load on the devices at objective lens up/down, suppressing vibrations by lowering the inertial force at objective lens switching, etc. (MPLFLN series, LMPLFLN series) UIS2 objective lenses ensure compatibility (screw diameter, optical performance) with the UIS optical system and have the following features compared to conventional objective lenses. 45 mm and the focal length of the tube lens is 180 mm. Infinity-corrected Optical System Objective Lens Eyepiece Parallel Light Beam Features of UIS2 Objective Lenses Objective Lens Tube Lens 1. Wavefront Aberration Control The Olympus UIS2 objective lenses set a new standard, with wavefront aberration control in addition to common performance standards of N.A. and W.D. Olympus challenges farther highest order optics which has not been fulfilled by the conventional standards. We offer excellent performance objective lenses by minimizing the aberrations that lower resolution. 5. Adoption of Eco-lens The glass materials of UIS2 objective lenses are all lead- and cadmium-free eco-glass. Based on our conviction that the UIS2 system is the best way to maximize the advantages of infinity-corrected optical systems, we confidently recommend the UIS2-featured Olympus microscope units for all your highprecision needs in research, inspection and production equipment. *Wave front aberration: refer to the optical terminology at the Intermediate Image Basic Dimensions of UIS2 Optical System end of this document. Eyepiece The UIS2 optical system optimally corrects aberration with a dedicated telan lens and an eyepiece so that the coma aberration and flatness are not degraded even when the telan lens exit pupil position is changed by changing the objective lens and telan distance. This makes it possible to use a distance of 50 mm to 170 mm from objective lens mounting position to the single port tube with lens. Intermediate Image Advantages of Infinity-corrected Optics This system, known as "infinity-corrected optics", offers a number of advantages: *Coma aberration: refer to the optical terminology at the end of • There is no change in magnification even when the distance between the objective lens and tube lens is altered. • With the total magnification remaining constant, there is no image aberration — even when prisms or sliders are interposed between the objective lens and the tube lens. this document. Figure 3 Basic Dimensions of UIS2 Optical System U-TLU (Single Port Tube with Lens) Objective Lens * 40 mm Image * 84 mm 45 mm Recommended Distance 50–170 mm As thousands of users have found by experience, these advantages are crucial to composing the ideal microscope optical system. What's more, it is even possible to freely insert or remove intermediate attachments in the parallel rays of 57.6 mm 102 mm 2. Objective Lenses with Excellent Image Parcentricity High power SemiApochromatic UIS2 objective lenses make the centration tolerance between objective lenses on the microscope nosepiece keep the image within the enter of the field of view even with digital cameras. (50x or higher power in both MPLFLN and LMPLFLN series) * Refer to the Olympus home page for detailed objective lenses specifications. 3. Improvement of Color Reproducibility UIS2 objective lenses realize natural color reproduction without any chromatic shifts using stringently selected high transmittance glass and advanced coating technology that provides high transmittance which is flat over an ultrawide band wavelength. In addition, since the total optical system, including the tube lens is designed to reproduce a natural color, clear images faithful to the specimen are obtained even with digital imaging. *Basic dimensions when our revolving nosepiece and illuminator are combined. When the position of the illuminator above is changed, illumination performance cannot be maintained. 3 4 System Diagram System Diagram BXFM System Diagram Motorized Unit System Diagram (refer to pages 40-44) Video System (refer to pages 38-39) Video Camera Video Camera C-mount B Mount 2/3" Video Camera S Mount 2/3" Video Camera F Mount Observation Tubes (refer to pages 30-31) U-AN (refer to pages 27-28) MX-AFDIC U-FMT U-CMAD3 U-BMAD U-AN360-3 Intermediate Tubes (refer to pages 33-34) U-SMAD U-TMAD U-AN U-FWO U-PO3 U-AFA2M-VIS U-LH100HGAPO U-LH100HG U-LH75XEAPO U-RFL-T U-RX-T U-POTP3 U-TV0.25xC U-TV0.35xC-2 U-TV0.5xC-3 U-TV0.63xC U-TV1x-2 Mirror Units U-25ND6 U-25ND25 U-25LBD U-25IF550 U-25L42 U-25FR U-FWR Observation/Single Tubes and Eyepieces (refer to pages 31-32, 35) WHN Eyepieces SWH Eyepieces Widefield Trinocular Observation Tubes Super Widefield Trinocular Observation Tubes TH4-HS U-TLU U-TLUIR U-D6REMC U-PO3 U-POIR (refer to page 37) (refer to pages 7-19) BF Objective Lenses U-DICRH Illumination Systems and Power Supply (refer to pages 24-30) U-AN TH4-100 U-ZPCB Z Board U-DICR U-AN360-3 U-AN360IR U-LH100-3 U-LH100L-3 BX-RLAA BX-RFAA BX-UCB* U-HSTR2 BD-M-AD U-25ND6, U-25ND25, U-25LBD, U-25IF550, U-25L42, U-25FR, U-BP1100IR, U-BP1200IR U-POTP3 Revolving Nosepiece (refer to pages 36-37) U-D5BDREMC U-D6BDREMC U-DICRHC BF/DF Objective Lenses PC See manual analySIS FIVE Control Software * BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 42). FS AS FS AS 5 6 SHUTTER ND BX-RLA2 BX-URA2 U-KMAS BX-KMA/ BX-KMA-ESD BXFM-A System Diagram SZX-TLGAD (refer to page 44) U-RCV LG-SF LG-PS2 (refer to page 27) ø32 Filter Video System (refer to pages 38-39) U-LGAD Focusing Units (refer to pages 20-23) Auxiliary Lens (provided with the BXFMA-F) Observation Tubes (refer to pages 31-32) U-DULHA MX-AFDIC TH4-100 U-LH100-3 U-LH100L-3 U-LH100IR BXFM-ILHS BXFM-ILH U-RFL-T U-RX-T U-LH100HG U-LH75XEAPO U-AN (refer to page 29) TH4-HS U-AFA2M-VIS LG-SF LG-PS2 U-LGAD BXFM-ILHSPU U-LH100HGAPO U-LH100HG U-LH75XEAPO BXFM-F U-RFL-T U-RX-T (refer to page 28) BXFMA-F Stands (refer to page 23) U-FWR U-LH100-3 U-LH100L-3 U-RMT BX-UCB U-ZPCB Z Board See manual U-D6REMC U-P5REMC U-D5BDREMC U-D6BDREMC U-P6BDREMC Objective Lenses SZ-STL U-ST U-HSTR2 U-IFFH PC U-FH SZ2-STU2 *Different types may be offered in each area. 5 6 analySIS FIVE Control Software UIS2 Objective Lenses UIS2 Objective Lenses ■ Meaning of Abbreviations Shown on Objective Lens M Plan Apochromat Metallurgical (no cover) Long Working Distance Metallurgical Use SLM: Super Long Working Distance Metallurgical Use LC: Observation Through Substrate PL: Plan/ Corrects Field Curvature of the Periphery of the Image Plane None: Achromat/ Corrects Aberration at 2 Wavelengths of Blue and Red FL: SemiApochromat/ Corrects Chromatic Aberration in the Visible Range (violet~red) APO: Apochromat/ Optimally Corrects Chromatic Aberration in the Entire Visible Band (violet~red) MPLAPON Series Number: Objective Lens Magnification None: Brightfield BD: Brightfield/Darkfield BDP: Brightfield/Darkfield/ Polarizing IR: IR LCD: LCD Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees* optical performance (wavefront aberration) with a Strehl ratio** of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit. MPLAPON50x MPLAPON100x ø20.32 ø20.32 4.5 M: LM: B D 4.5 M P L (Plan) F L N - 1 0 0 * Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23°C±1°C; assessment: measurement within the 97% range of the pupil dia.). ** Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system. 38.43 42.4 42.78 41 45 For Brightfield Observation (44.65) Cover Glass Thickness (no cover) 37.98 45 Objective Lenses Series Abbreviation (PL: Plan) N.A. (Numerical Aperture) (44.65) Magnification Infinity-corrected Optical System 41.62 ■ Objective Lens Notation Field Number ø8.8 F.N. (Field Number) 26.5 26.5 1.25x: 22 /2.5x: 26.5 \U \L \ \ \*3 \ \L \U aU \L \ a \ \*3 \*3 \ ø20.3 ø28.5 Use together with polarizer and analyzer recommended. for near-IR observation for near-IR observation *1 DIC Prism U-DICR: UM/LM Position, U-DICRHC: LM Position Fixed, U-DICRH: UM Position Fixed. *2 40x: BF Only *3 5–20x: U Excitation Also Possible \: Responds a: Optimally Responds BF: Brightfield DF: Darkfield DIC: Differential Interference Contrast POL: Polarized light FL: Fluorescence MPLAPON 50x MPLAPON 100x Numerical Aperture Widefield Eyepiece WHN10x Field Number 22 Working Distance Focal Distance f (mm) (mm) 0.95 0.95 Unit: mm ø28 UIS2 Objective Lenses Objective Lens (magnification) for LCD ø16 ø20.2 0.35 0.35 3.6 1.8 Weight (g) 139 125 Super Widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 500 1000 0.44 0.22 1.0 0.67 500 1000 0.53 0.27 1.0 0.7 Screw: W20.32x0.706 (0.8"x1/36") M Plan Apochromat MPLAPON 100xO ■ Features of Objective Lens Series a MPLAPON Series: M Plan Apochromat — P 8 Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees* optical performance (wavefront aberration) with a Strehl ratio** of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit. a LCPLN-IR Series: IR Long WD M Plan Achromat — P 15 Exclusive for the near-infrared microscopy largely of the internal structure in silicon wafers, LCPLN-IR series has correction collar for aberration dependent on thickness of silicon or glass substrate. a MPLFLN-BD Series: M Plan SemiApochromat BD — P 16 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLFLN-BDP Series: M Plan SemiApochromat BDP — P 17 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations. a LMPLFLN-BD Series: Long WD M Plan SemiApochromat BD — P 18 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN-BD Series: M Plan Achromat BD — P 19 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. * Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23°C±1°C; assessment: measurement within the 97% range of the pupil dia.). ** Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system. *** Specified oil: IMMOIL-F30CC a MPLAPON 100xO: M Plan Apochromat — P 8 This is a Plan Apochromat objective lens of the oil-immersion type*** that features a numerical aperture of 1.4. It provides the highest level of chromatic aberration correction and a high resolving power. a MPLFLN Series: M Plan SemiApochromat — P 9 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 8 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1 mm or longer (except 40x). Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification (40x is not applicable to DIC observation). For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator. a LMPLFLN Series: Long WD M Plan SemiApochromat — P 10 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN Series: M Plan Achromat — P 11 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. a LCPLFLN-LCD Series: LCD Long WD M Plan SemiApochromat — P 12 Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction collar. a SLMPLN Series: Super Long WD M Plan Achromat — P 13 Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN Series. a LMPLN-IR Series: IR Long WD M Plan Achromat — P 14 Objective lens series exclusive for the near-infrared microscopy largely of the internal structure in silicon wafers. This is a Plan Apochromat objective lens of the oil-immersion type*** that features a numerical aperture of 1.4. It provides the highest level of chromatic aberration correction and a high resolving power. MPLAPON100xO ø20.32 36.909 \ \ \ \ 26.5 26.5 22 26.5 26.5 22 22 26.5 26.5 26.5 22 Remarks 41.676 \ \ \ \ \ \ \ \ \ \ \ FL 43.496 5/10/20/40*2/50/100 5/10/20/50/100 5/10/20/50/100 20/50/100 20/50/100 5/10 20/50/100 5/10/20/50/100/150 5/10/20/50/100 5/10/20/50/100 5/10/20/50/100 POL \ W.D.=0.35 \ DIC*1 \U 4.5 1.25/2.5 DF 45 LMPLFLN MPLN LCPLFLN-LCD SLMPLN LMPLN-IR LCPLN-IR MPLFLN-BD MPLFLN-BDP LMPLFLN-BD MPLN-BD BF \ \ (44.9) MPLAPON MPLAPON O MPLFLN Magnification 50/100 100 *** Specified Oil: IMMOIL-F30CC ø6.6 ø17.5 W.D.=0.1 Series ø5.5 ø18.9 W.D.=0.35 ■ Objective Lens Series List ø22 ø30 Unit: mm Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) MPLAPON 100xO Numerical Aperture 1.4 Working Distance Focal Distance f (mm) (mm) 0.1 1.8 Weight (g) 158 7 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 1000 Screw: W20.32x0.706 (0.8"x1/36") 8 Super Widefield Eyepiece SWH10x Field Number 26.5 0.22 0.59 1000 0.27 0.59 UIS2 Objective Lenses UIS2 Objective Lenses M Plan SemiApochromat Long WD M Plan SemiApochromat MPLFLN Series LMPLFLN Series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 8 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1 mm or longer (except 40x). Since the exit pupil position of the 5x–100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification (40x is not applicable to DIC observation). For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator. Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x–100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. ø20.32 LMPLFLN10x LMPLFLN20x ø20.32 ø20.32 ø20.32 ø20.32 ø20.32 ø17.8 45 29.31 (33) 22.1 (24) 45 ø17.8 ø12.5 ø18.2 ø26 ø15 ø18.1 ø26 ø26 ø26 4.7 45 (34.4) ø15.2 W.D.=12 W.D.=21 W.D.=22.5 ø20.32 W.D.=10.6 ø15.2 ø25.8 ø20.32 45 38.4 42.4 42.61 ø7.8 W.D.=1 ø16 LMPLFLN100x 4.9 4.8 45 (44) 36.8 40.8 41.78 45 (44) W.D.=1 40.5 41.4 45 35.6 ø10.7 ø26 LMPLFLN50x ø20.32 4.8 4.5 ø8.5 W.D.=0.63 (44.37) 35.1 W.D.=3.1 MPLFLN100x ø22.4 37.26 ø20.32 ø26 ø17 ø15.2 ø26 W.D.=3.4 MPLFLN50x ø14.4 22.2 (22.5) MPLFLN40x ø26 45 45 ø26 ø25.4 30.4 ø30 W.D.=11 ø29 ø14.5 MPLFLN20x ø12.1 28.41 (34) 22.55 45 ø20.9 W.D.=20 ø30 4.5 4.5 31.6 ø24.5 W.D.=10.7 ø29 4.5 (41.9) 45 (25) 4.9 45 41.2 (34.3) 4.9 (41.5) 45 W.D.=3.5 ø28.6 4.8 ø20.32 LMPLFLN5x 41.1 ø28 ø20.32 MPLFLN10x 4.9 ø28 ø20.32 MPLFLN5x 4.5 MPLFLN2.5x* (41.6) MPLFLN1.25x* (W.D.: Working Distance) ø26 Unit: mm Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) Numerical Aperture MPLFLN 1.25x 0.04 MPLFLN 2.5x 0.08 MPLFLN 5x 0.15 MPLFLN 10x 0.30 MPLFLN 20x 0.45 MPLFLN 40x 0.75 MPLFLN 50x 0.80 MPLFLN 100x 0.90 Screw: W20.32x0.706 (0.8"x1/36") Working Distance Focal Distance f (mm) (mm) 3.5 10.7 20.0 11.0 3.1 0.63 1.0 1.0 145 72 36 18 9 4.5 3.6 1.8 Weight (g) 122 106 51.5 68.1 70.4 120 89.9 90.9 Unit: mm Super widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 12.5 25 50 100 200 400 500 1000 17.6 8.8 4.4 2.2 1.1 0.55 0.44 0.22 870 220 59 15 5.2 1.66 1.3 0.73 — 25 50 100 200 400 500 1000 — 10.6 5.3 2.7 1.3 0.66 0.53 0.27 — 220 59 15 5.1 1.66 1.3 0.73 Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective lens (magnification) LMPLFLN LMPLFLN LMPLFLN LMPLFLN LMPLFLN 5x 10x 20x 50x 100x Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working Distance Focal Distance f (mm) (mm) 22.5 21.0 12.0 10.6 3.4 36 18 9 3.6 1.8 Weight (g) 50 54 73 77 94 9 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 50 100 200 500 1000 Screw: W20.32x0.706 (0.8"x1/36") 10 Super Widefield Eyepiece SWH10x Field Number 26.5 4.4 2.2 1.1 0.44 0.22 70 18 6.1 2.5 0.87 50 100 200 500 1000 5.3 2.7 1.3 0.53 0.27 70 18 6.1 2.5 0.87 UIS2 Objective Lenses UIS2 Objective Lenses M Plan Achromat LCD Long WD M Plan SemiApochromat MPLN Series LCPLFLN-LCD Series Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction collar. MPLN20x LCPLFLN20xLCD*** LCPLFLN50xLCD*** LCPLFLN100xLCD*** ø20.32 ø20.32 ø20.32 ø20.32 ø20.32 ø20.32 ø24 ø31 20 27.5 ø15.2 ø25 t=0.7 41.6 43.4 45.238 * t=0.7 ø25 (43.638) 25 38.65 40.45 20 4.8 (42.038) 45.238 * 24.5 34.75 20 ø29.5 ø17.84 W.D.=0.9 ø24 ø25 ø12.77 W.D.=2.5 ø15.8 ø15 t=0.7 ø11.9 ø16 36.55 4.5 W.D.=7.8 ø6 (36.738) 45.238 * 37.2 41.3 42.4 45 ø12 W.D.=1.3 ø24 (43.7) 28.8 33.6 32.71 4.5 ø10.5 W.D.=10.6 (34.4) 45 23.4 4.5 (25) 45 W.D.=20 ø21 4.5 MPLN10x 4.5 MPLN5x ø29.5 ø31 ø29.5 Unit: mm ø31 * Value at glass thickness 0.7 mm observation. MPLN100x ø20.32 ø20.32 Objective Lens Corresponding Glass Thickness (mm) Correction Collar Indication Working Distance (mm) Correction System ø15.8 W.D.=0.21 W.D.=0.38 ø11.9 1.2 7.4 0 3.0 Numerical Aperture LCPLFLN 20xLCD 0.45 LCPLFLN 50xLCD 0.70 LCPLFLN 100xLCD 0.85 Screw: W20.32x0.706 (0.8"x1/36") ø4.4 ø11.6 ø15.6 Working Distance** Focal Distance f (mm) (mm) Unit: mm Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) MPLN 5x MPLN 10x MPLN 20x MPLN 50x MPLN 100x Numerical Aperture 0.10 0.25 0.40 0.75 0.90 Working Distance (mm) 20.0 10.6 1.3 0.38 0.21 1.2 2.2 0 1.2 LCPLFLN100xLCD 0–0.7 0.5 0.98 Correction Collar 0.7 0.9 Focal Distance f (mm) 36 18 9 3.6 1.8 Weight (g) 64 80 111 113 116 Total Practical Field of Depth of Focus Magnifications View (mm) (µm) 50 4.4 98 100 2.2 18 200 1.1 6.1 500 0.44 1.4 1000 0.22 0.73 Screw: W20.32x0.706 (0.8"x1/36") 11 Super widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Weight Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) (g) 7.8 9 146 200 1.1 5.2 200 1.3 5.2 2.5 3.6 170 500 0.44 1.6 500 0.53 1.6 0.9 1.8 185 1000 0.22 0.79 1000 0.27 0.79 **The figure shown here is the value when the correction collar indication is 0.7. *** To be available in the beginning of 2007 ø24 ø24 LCPLFLN50xLCD 0–1.2 0.7 2.5 Correction Collar Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) ø6 0 8.3 LCPLFLN20xLCD 0–1.2 0.7 7.8 Correction Collar 38.7 42.8 43.16 4.5 45 (44.79) 37.2 41.3 42.62 (44.62) 45 4.5 MPLN50x 12 UIS2 Objective Lenses UIS2 Objective Lenses Super Long WD M Plan Achromat IR Long WD M Plan Achromat SLMPLN Series LMPLN-IR Series Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN series. Objective lens series exclusive for the near-infrared microscopy largely of the internal structure in silicon wafers. LMPLN5xIR LMPLN10xIR ø20.32 ø20.32 ø20.32 4.5 (27.05) 20 45 (21.5) WD=18 ø16.9 ø14.7 ø20.6 ø25 ø17.3 ø23.8 ø24 ø26 WD=7.6 ø16.1 WD=18 WD=25 WD=23 45 36.2 37.2 4.9 (37.4) 24.1 45 45 (27) 26.3 19.3 19.8 4.9 4.9 45 (20) SLMPLN100x ø20.32 4.5 SLMPLN50x ø20.32 18 SLMPLN20x ø21.4 ø14.4 ø22 ø26 ø26 ø26 Unit: mm Unit: mm UIS2 Objective Lenses Objective Lens (magnification) Numerical Aperture SLMPLN 20x 0.25 SLMPLN 50x 0.35 SLMPLN 100x 0.6 Screw: W20.32x0.706 (0.8"x1/36") Working Distance Focal Distance f (mm) (mm) 25 18 7.6 9 3.6 1.8 Weight (g) 56 74 100 13 Widefield Eyepiece WHN10x Super Widefield Eyepiece SWH10x Field Number 22 Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 200 500 1000 1.1 0.44 0.22 11.4 4.2 1.3 200 500 1000 1.3 0.53 0.27 11.4 4.2 1.3 UIS Objective Lenses Objective Lens (magnification) LMPLN 5xIR LMPLN 10xIR Screw: W20.32x0.706 (0.8"x1/36") Numerical Aperture 0.1 0.3 Working Distance (mm) 23 18 14 Focal Distance f (mm) 36 18 Weight (g) 55 78 UIS2 Objective Lenses UIS2 Objective Lenses IR M Plan Achromat M Plan SemiApochromat BD LCPLN-IR Series MPLFLN-BD Series Exclusive for the near-infrared microscopy largely of the internal structure in silicon wafers, LCPLN-IR series has correction collar for aberration dependent on thickness of silicon or glass substrate. Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. LCPLN20xIR LCPLN50xIR LCPLN100xIR ø20.32 MPLFLN5xBD MPLFLN10xBD MPLFLN20xBD ø26 ø26 ø26 4.5 4.5 ø25 ø29.5 ø25 ø29.5 ø31 ø29.5 ø31 ø16 W.D.=12 ø17.82 ø22.4 ø29.5 35.97 39 39.5 (42) 45 34.1 37 36.5 ø16.8 ø17 W.D.=3 ø15.2 ø25 (38.5) 45 45 WD=1.2 WD=4.5 ø17.7 W.D.=6.5 31 30.25 (33) 31.5 19.8 43.4 45 43.8 27.5 19.8 25 29.5 31.2 (40.5) 45 19.88 24.38 34.4 (36.62) 45 WD=8.3 ø14.64 ø14.94 4.5 4.5 ø20.32 4.5 4.5 ø20.32 (BD: Brightfield/Darkfield) ø22 ø26.2 ø32 ø31 ø22 ø27.5 ø27 ø28.5 ø32 ø32 Unit: mm MPLFLN50xBD MPLFLN100xBD MPLFLN150xBD ø26 ø26 ø26 4.5 4.5 *With the use of 1100 nm laser. 41 45 (44) 1 1.0 41 0 1.2 45 1.2 4.1 (44) 0 4.5 LCPLN100xIR 0–1.0 0.5 1.1 Correction Collar 41 1.2 8.0 LCPLN50xIR 0–1.2 0.6 4.3 Correction Collar 45 0 8.3 LCPLN20xIR 0–1.2 0.7 8.2 Correction Collar (44) Objective Lens Corresponding Silicon Thickness (mm) Correction Collar Indication Working Distance* (mm) Correction System 4.5 Silicon thickness correction LCPLN50xLCD 0–1.2 1.2 7.6 0 4.5 1.2 3.7 Correction Collar LCPLN100xLCD 0–0.7 0 1.2 0.7 0.9 Correction Collar ø20 ø27.2 ø32 ø20 ø27.2 W.D.=1 0 8.3 LCPLN20xLCD 0–1.2 0.7 7.9 Correction Collar W.D.=1 Objective Lens Corresponding Glass Thickness (mm) Correction Collar Indication Working Distance* (mm) Correction System W.D.=1 Silicon thickness correction ø32 ø20 ø27.2 ø32 *With the use of 1064 nm laser. Unit: mm UIS Objective Lenses Numerical Working Distance* Objective Lens Aperture* (mm) (magnification) LCPLN 20xIR 0.45 8.3 LCPLN 50xIR 0.65 4.5 LCPLN 100xIR 0.85 1.2 Screw: W20.32x0.706 (0.8"x1/36") * The figure shown here is the value when the correction collar indication is 0. Focal Distance f (mm) 9 3.6 1.8 Weight (g) 149 169 184 Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) MPLFLN 5xBD MPLFLN 10xBD MPLFLN 20xBD MPLFLN 50xBD MPLFLN 100xBD MPLFLN 150xBD Numerical Aperture 0.15 0.30 0.45 0.80 0.90 0.90 Working Distance Focal Distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 1.0 1.2 Weight (g) 95.5 82.8 87.7 99.8 98.9 104.8 Screw: W26x0.706 15 16 Super Widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 50 4.4 59 50 5.3 59 100 2.2 15 100 2.7 15 200 1.1 5.2 200 1.3 5.2 500 0.44 1.3 500 0.53 1.3 1000 0.22 0.73 1000 0.27 0.73 1500 0.15 0.6 1500 0.18 0.6 UIS2 Objective Lenses UIS2 Objective Lenses M Plan SemiApochromat BDP (BDP: Brightfield/Darkfield/Polarizing) Long WD M Plan SemiApochromat BD (BD: Brightfield/Darkfield) MPLFLN-BDP Series LMPLFLN-BD Series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations. Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. MPLFLN20xBDP LMPLFLN5xBD LMPLFLN10xBD LMPLFLN20xBD ø26 ø26 ø26 ø26 ø26 ø26 31.3 32.2 32.5 4.5 (33) 45 ø32 ø32 ø32 ø32 LMPLFLN100xBD ø32 36.5 37.3 37.7 4.7 (41.7) 45 ø23 ø28 W.D.=3.3 ø20.7 W.D.=10.6 ø27.5 ø26 31.8 33 32.7 4.8 45 41 (34.4) 4.5 45 (44) W.D.=12 ø32 ø20 W.D.=1 30.2 ø30 ø21 ø29 ø30.3 ø32 Unit: mm Unit: mm Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Numerical Aperture 0.15 0.25 0.40 0.75 0.90 Working Distance Focal Distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 ø28 ø30 ø32 Objective Lens (magnification) MPLFLN 5xBDP MPLFLN 10xBDP MPLFLN 20xBDP MPLFLN 50xBDP MPLFLN 100xBDP ø23 ø29.5 ø26 41 ø28.2 ø28.5 ø26 W.D.=1 ø21.9 ø27 ø26 ø32 32 45 ø28 LMPLFLN50xBD ø20 W.D.=10 ø27.5 ø22.4 MPLFLN100xBDP ø27.5 32.3 (35) 24 26.2 W.D.=15 ø26 ø22 ø15.5 ø16.2 ø22 MPLFLN50xBDP 4.5 (44) 26.6 5 (30) 45 35.97 39 39.5 W.D.=3 ø22 ø32 45 (42) 45 34.1 37 36.5 4.5 4.5 (38.5) 45 ø29.3 ø17 ø16.8 W.D.=6.5 31 30.25 31.5 (33) 45 W.D.=12 ø16 ø22.4 4.5 MPLFLN10xBDP 4.5 MPLFLN5xBDP Weight (g) 95.5 83.3 88.5 100.5 101.5 Screw: W26x0.706 Super Widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 50 4.4 59 50 5.3 59 100 2.2 18 100 2.7 18 200 1.1 6.1 200 1.3 6.1 500 0.44 1.4 500 0.53 1.4 1000 0.22 0.73 1000 0.27 0.73 Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) LMPLFLN 5xBD LMPLFLN 10xBD LMPLFLN 20xBD LMPLFLN 50xBD LMPLFLN 100xBD Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working Distance Focal Distance f (mm) (mm) 15.0 36 10.0 18 12.0 9 10.6 3.6 3.3 1.8 Weight (g) 81 84 86 85 102 Screw: W26x0.706 17 18 Super Widefield Eyepiece SWH10x Field Number 26.5 Total Practical Field Depth of Total Practical Field Depth of Magnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm) 50 4.4 70 50 5.3 70 100 2.2 18 100 2.7 18 200 1.1 6.1 200 1.3 6.1 500 0.44 2.5 500 0.53 2.5 1000 0.22 0.87 1000 0.27 0.87 UIS2 Objective Lenses Microscope System BXFM M Plan Achromat BD (BD: Brightfield/Darkfield) BXFM Frame MPLN-BD Series BXFM-F Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit. Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole. 84 36 82 36 4–M4 Depth 9 35 ø26 ø26 (4.5) 4.5 55 124 0.5 0.5 4–M4 Depth 7 66.2 17 Pillar Mount Hole Center (13) 17 41 42.5 45 (43.7) 80 34.01 37 36.5 45 (38.5) 31 30.25 31.5 45 (33) 100 17 4.5 34 23 ø26 4-M8 Depth 8 Bolt Mount Screw 7 58.5 MPLN20xBD (13) MPLN10xBD Stroke MPLN5xBD ø16.8 ø17 16 ø16 ø30.5 W.D.=1.3 W.D.=6.5 W.D.=12 2A00002 ø29.3 ø22 ø26 ø32 ø23.6 ø29 ø27 ø32 ø3 2H Pil 8 lar Mo ø32 un 98 MPLN50xBD tH MPLN100xBD Weight: 1.9kg ø26 41 42.5 43.33 43.71 45 (44.79) 41 42.5 43.68 45 (44.62) 4.5 4.5 ø26 ø10 ø23.1 W.D.=0.21 W.D.=0.38 ø10 ø20.7 ø23 ø27 ø29 ø29 ø32 ø32 Unit: mm Widefield Eyepiece WHN10x Field Number 22 UIS2 Objective Lenses Objective Lens (magnification) MPLN MPLN MPLN MPLN MPLN 5xBD 10xBD 20xBD 50xBD 100xBD Numerical Aperture 0.10 0.25 0.40 0.75 0.90 ole 110 Working Distance (mm) 12.0 6.5 1.3 0.38 0.21 Focal Distance f (mm) Weight (g) 36 18 9 3.6 1.8 137 155 162 157 160 Total Practical Field of Depth of Focus Magnifications View (mm) (µm) 50 100 200 500 1000 4.4 2.2 1.1 0.44 0.22 98 18 6.1 1.4 0.73 Screw: W26x0.706 19 20 Unit: mm Microscope System BXFM Microscope System BXFM BXFM-S BXFM-F + BXFM-ILH + BXFM-ILHSPU BXFM-F + BXFM-ILHS Accommodates the reflected light brightfield/darkfield and fluorescence illuminators. Compact focusing unit suitable for building into existing equipment. 180 84 BXFM Light Axis 165 130 141 106 Holder Mounting Position 59 Holder Mounting Position 83 Light Axis Stroke 20 45 23 Objective Lens Mounting Position Objective Lens Mounting Position 11 45 72 23 Revolving Nosepiece Mounting Position 40 Revolving Nosepiece Mounting Position 40 Stroke 7 3.5 7 Light Axis Pillar Axis Pillar Axis Weight: 2.4kg Specimen Position Weight: 3.2kg BXFM Combination Sample BXFM-F + BXFM-ILH + BXFM-ILHSPU + TR30-2 + BX-RLA2 + U-LH100L-3 Unit: mm Specimen Position Unit: mm BXFM-S Combination Sample BXFM-F + BXFM-ILHS + TR30-2 + U-KMAS + U-LH100L-3 165 130 87.5 83 290 187 92.5 106 ø32 220 169 20 40 45 19-49 Stroke Specimen Surface 180 124 17-47 Stroke 124 45 11 40 3.5 249 ø 32 Specimen Surface 84 208 587 * For installation dimensions, refer to those for the BXFM-F. Weight: 8.2kg (exclude objective lens) 21 Unit: mm * For installation dimensions, refer to those for the BXFM-F. Weight: 5.5 kg (exclude objective lens) 22 Unit: mm Microscope System BXFM Illumination Units Stands Reflected Light Illuminator for BF/DF A wide variety of stands are available to suit different applications and purposes. BX-RLA2 ND filters are linked when exchanging between brightfield and darkfield. 300 350 4 Stroke Horizontal: 234 mm, Vertical: 205 mm 5 Movement Range Horizontal: 541 (435+106) mm max. (vertical pole — BXFM-S optical axis) 6 Maximum Specimen Weight Forward: 10 kg (within 90-degree area) Transverse Direction: 6 kg Backward Direction: 7 kg (at maximum stroke) 622 º 90 30 ø25 180º 214~435 7 Weight U-DICRHC 130 35 ø75 88 107.5 46 108 16.2 12 79 50 130 130 29.4 Revolving Nosepiece Mounting Position Illuminator Mounting Position 38.7 56.8 45 265 335 Weight: 3.4 kg 30 kg 503 Ø32 U-AN360-3 U-AN U-DICR U-DICRH 13 ø25 mm (both upper and lower poles) 11.8 ø40 mm 3 Horizontal Poles Diameters 17 2 Vertical Pole Diameter 7.5 26 Specifications ø32 mm 84 300 250 Item 1 Diameter of Focusing Arm or Fixing Section of Tube Weight (g) 20 20 20 20 20 20 71 71 41 Major Specifications Description LBD Filter Slider IF550 Filter Slider ND Filter ND Filter Frost Filter Slider UV-cut Filter Polarizer Slider for Reflected Light Polarizer Slider for Reflected Light with Tint Plate 360° Rotatable Analyzer Slider Analyzer Slider for Reflected Light DIC Slider for Reflected Light DIC Slider for Reflected Light (high resolution type) DIC Slider for Reflected Light (high contrast type) 3.5 Unit Name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 30 Accessories 17.5 SZ2-STU2 Universal Stand Type 2 31.5 30 144 ø40 50.5 130 Universal Reflected Light Illuminator BX-URA2 * The rotation angle of the horizontal arm can restrict to 90 degrees with stopper. Suitable for observations ranging from brightfield to fluorescence. Six mirror units can be attached to this reflected light illuminator simultaneously. SZ-STL Large Stand U-DICRHC ø32 143 U-MBF3 U-MDF3* U-MDIC3 U-MBFL3 133 267 ø32 U-MWBS3 45 20 49 46 U-MWUS3 17.5 U-MWGS3 Weight: 1.8 kg Weight: 5 kg 23 Unit: mm ø84 88 11.6 11.6 12 17 76 Revolving Nosepiece Mounting Position 27.2 41 73 84 160 106 86.8 3.5 320 133 88 400 35 ) U-AN360-3 U-AN U-DICR U-DICRH ø110 Description Weight (g) LBD Filter Slider 20 IF550 Filter Slider 20 ND Filter 20 ND Filter 20 Frost Filter Slider 20 UV-cut Filter 20 Polarizer Slider for Reflected Light 71 Polarizer Slider for Reflected Light 71 with Tint Plate 360° Rotatable Analyzer Slider 79 Analyzer Slider for Reflected Light 50 DIC Slider for Reflected Light 130 DIC Slider for Reflected Light 130 (high resolution type) DIC Slider for Reflected Light 130 (high contrast type) Mirror Unit for Reflected Brightfield 80 Mirror Unit for Reflected Darkfield 80 Mirror Unit for Reflected DIC 80 Mirror Unit for Reflected Brightfield, 80 for High Intensity Light Source Fluorescence Mirror Unit for 80 Reflected (U excitation) Fluorescence Mirror Unit for 80 Reflected (B excitation) Fluorescence Mirror Unit for 80 Reflected (G excitation) .9° Unit Name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 (152) Accessories (17 U-ST Compact Stand 5° 41 13 Illuminator Mounting Position 261 367 Weight: 3.8 kg * U-RCV (DF converter for BX-URA2) is needed with darkfield observation. Unit: mm 24 Illumination Units Illumination Units Reflected Light Illuminators for BF Reflected Light Illuminator for BF BX-KMA/BX-KMA-ESD U-KMAS Enables brightfield, Nomarski DIC and simple polarizing observations. ESD model is also available. Very compact reflected light illuminator with reduced depth. U-DICRHC 130 Cable Length 260.5 mm U-DICRHC Illuminator Mounting Position ø75 88 Weight (g) 20 20 20 20 20 20 71 71 79 50 130 130 64 88 ø70 U-AN360-3 U-AN U-DICR U-DICRH Description LBD Filter Slider IF550 Filter Slider ND Filter ND Filter Frost Filter Slider UV-cut Filter Polarizer Slider for Reflected Light Polarizer Slider for Reflected Light with Tint Plate 360° Rotatable Analyzer Slider Analyzer Slider for Reflected Light DIC Slider for Reflected Light DIC Slider for Reflected Light (high resolution type) DIC Slider for Reflected Light (high contrast type) 21 Revolving Nosepiece Mounting Position Unit Name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 41 79 50 130 130 108 Weight (g) 20 20 20 20 20 20 71 71 84 U-AN360-3 U-AN U-DICR U-DICRH Accessories Description LBD Filter Slider IF550 Filter Slider ND Filter ND Filter Frost Filter Slider UV-cut Filter Polarizer Slider for Reflected Light Polarizer Slider for Reflected Light with Tint Plate 360° Rotatable Analyzer Slider Analyzer Slider for Reflected Light DIC Slider for Reflected Light DIC Slider for Reflected Light (high resolution type) DIC Slider for Reflected Light (high contrast type) 3.5 Unit Name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 6 Accessories 155 198 130 21 Weight: 1.2 kg 30 250 312.5 * Combine SZX-TLGAD when using fiber illumination. Weight: 3.1kg Unit: mm Unit: mm Mounting Dimensions of Illuminators (BX-RLA2, BX-URA2 and BX-KMA/BX-KMA-ESD) 100 (±0.1) 21 36 (±0.1) 170 Distance to the Light Axis 2 5.5 4-M5 Depth 12 or more (6) (11) Location Face 37 ±0.1 45° Location Face 45° 2.5 (±10 ') 62 (±0.1) 5 52 Revolving Nosepiece Relief Dimension 3.5 Location Face ss Le an th 82 Revolving Nosepiece Relief Dimension Fix illuminator using four M5 screws and projection for fastening. R6 Convex Section (2-4) for Positioning Unit: mm 25 26 Light Source Units Light Source Units Lamp Housings Halogen Illumination Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose. For the 100 W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch, both are located close to the operator's hand, are provided. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. U-LH75XEAPO 75 W Xenon Apo Lamp Housing (148.5) 30.2 (148.5) 83.5 65 65 U-LH100-3/U-LH100IR/U-LH100L-3 100 W Halogen Lamp Housings U-LH100HGAPO 100 W Mercury Apo Lamp Housing U-LH100HG 100 W Mercury Lamp Housing (30.2) 83.5 65 93 93 107 65 8 8 40.8 40.8 20 Cable Length U-LH100-3: 290 mm U-LH100IR: 290 mm U-LH100L-3: 800 mm Accepted Lamp: 12V100WHAL (high intensity lamp) 12V100WHAL-L (long life lamp) 85.5 75 75 55 6 20 115 30° 25° 115 30° 25° 130 169 (depth dimension for installation) 70 130 180.5 169 (depth dimension for installation) 18.5 * External power supply (TH4-100 or TH4-200) and power cable (UYCP) are necessary for 100 W halogen lamp housings. These items are sold separately. 180.5 Accepted Lamp: UXL-75XB Weight: 3.1 kg Cable Length 2,000 mm Accepted Lamp: USH-103OL Weight: 2.7 kg 37 Cable Length 2,000 mm *Power supply unit (U-RX-T) and power cable (UYCP) are necessary for 75 W xenon lamp housing. These items are sold separately. * Power supply unit (U-RFL-T) and power cable (UYCP) are necessary for 100 W mercury lamp housings. These items are sold separately. Weight: 880 g 135 Depth Dimension for Installation 146.5 U-RX-T Power Supply Unit for Xenon Lamp U-RFL-T Power Supply Unit for Mercury Lamp TH4-100/200 External Power Supply TH4-HS Hand Switch Cable Length: 2,000 mm 90 ±1 58.2 18.5 31.2 31 186 ±1 115 31 Weight: 140 g Weight: 2.2 kg 42 75 14.5 38 200 53 150 122 65.7 180 ±1 77 120 125 270±1 Weight: Approximately 3 kg U-RMT Extension Cord Weight: 200 g Unit: mm 27 1,700 28 Unit: mm Light Source Units Light Source Units Halogen Fiber Illumination Accessories Lamp Housing Accessory All Olympus reflected light illuminators can be used with fiber illumination. Two lamp housings can be attached simultaneously. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. U-RCV DF Converter for BX-URA2 U-LGAD Fiber Adapter for Reflected Light Observation SZX-TLGAD Transmitted Light Guide Adapter U-DULHA Double Lamp House Adapter ø12 (42.5) 62.5 Weight: 315 g 24 44 Dimension for Installation Dimension for Installation * Mountable with BX-KMA/BX-KMA-ESD only. Light Guide Mount Hole ø12 Weight: 390 g ø14 Light Guide Mount Hole ø12 Weight: 135 g 0 171 51 Dimensions for Installation 88 26 31.5 202 ø49 ø30 ø32 ø67 ø59 ø75 21 Darkfield light excluding tube which is built into the BX-URA2. LG-PS2* Light Source Weight: 1.2 kg ø15 Light Guide Mounting Position 8 235 10 86 130 76 10 126 251 *The types of model varies by country in use. Weight: 1.6 kg LG-SF Light Guide Groove: Width 3, Depth 1 10 61 29 ø13 ø25 30 31 ø10.1 ø12 ø15 37.5 ø59 43 82 1,000 25 Weight: 210 g 20 Unit: mm Unit: mm 30 Observation Tubes Observation Tubes Widefield Trinocular Observation Tubes Super Widefield Trinocular Observation Tubes Trinocular observation tubes with widefield of view. Compatible with F.N. 22. Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5. U-SWTR-3 Super Widefield Trinocular Tube U-SWETR Super Widefield Erect Image Trinocular Tube 163.1 199.9 224 73 83.4 92.9 36.5 19 14 82.3* 62.5 103.9 104.9 92 43.5 76.4* 60.6 55.6 79.6 98.8 150.5 186.6 (IR: 188.9) 95.8 47.9 62.5 (IR: 64.5) 63.1 18 92.5* (IR: 93.9) 59.65 33.5 16 120 180.8 U-ETR-4 Widefield Erect Image Trinocular Tube 175 U-TR30-2/ Widefield Trinocular Tube U-TR30IR Widefield Trinocular Tube for IR 51.6 68.6 173.8 220.8 49.9 98.6 62 201.9 248.9 Unit: mm Name U-TR30-2 U-TR30IR U-ETR-4 Field Number (F.N.) Inclination Angle (degree) Interpupillary Distance (mm) Light Path Selector (eyepiece/video port) Observation Image Weight (g) 22 22 22 30 30 30 50-76 50-76 50-76 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100 Inverted Inverted Erect 1600 1600 1900 MX-SWETTR Super Widefield Erect Image Tilting Trinocular Tube 139.400 61.981 117.223 *Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62 mm. Single Port Tube with Lens When the visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens can be attached directly to the video port. 88.000 95.929 101.929 2.837 57.6 ø60 70.330 20° 0' 0" 42° U-TLU Single Port Tube with Lens U-TLUIR Single Port Tube with Lens for IR 149.081 0' 0 " 318.527 328.338 337.718 72.200 Unit: mm Weight: 350 g Name • For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6). Unit: mm U-SWTR-3 U-SWETR MX-SWETTR Field Number (F.N.) 26.5 26.5 26.5 Inclination Angle (degree) Interpupillary Distance (mm) Light Path Selector (eyepiece/video port) Observation Image Weight (g) 24 24 0-42 50-76 50-76 50-76 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100 Inverted Erect Erect 2300 4200 4200 *Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62 mm. 31 32 Intermediate Tubes & Accessories Intermediate Tubes & Accessories Intermediate Tubes Various accessories for various observation need. U-CA Magnification Changer Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications. U-ECA Magnification Changer 2x Provides 1x and 2x intermediate magnifications. U-DP Dual Port Use this intermediate tube to divide the light path. U-DP1xC Dual Port 1x Combine with U-DP to obtain a 1x image. ø140 ø138 88 ø140 88 ø44 ø30 150 151 ø44 ø25 17.53 1-32UN 4.5 170.5 Mount Face 182 Weight: 500 g 96 42 51 Mount Face 42 45 37 38 ø75 Weight: 1.3 kg 57 52 ø70 Weight: 1.3 kg Weight: 1 kg U-TRU Trinocular Intermediate Attachment Intermediate attachment which divides the light path, allowing attachment of both digital and video cameras. Light Path Selector by Mirror Unit ø140 Transmitted Side Port: Side Port = 70:30 (with use of U-MBF3) 37 21.2 89 150 (8°) U-EPA2 Eyepoint Adjuster Raises eyepoint by 30 mm. 115 U-APT Arrow Pointer Projects an arrow into the field of view. Transmitted Side Port: Side Port = 100:0 183.9 106.9 (45°) 15V0.2A 0.92 Tolerence from Light Axis 30 52 58.2 45 88 120 45.3 Weight: 1.2 kg BI:PT=100:0/20:80 Weight: 1.3 kg 33 Unit: mm Weight: Approximately 500 g Unit: mm 34 Eyepieces/Filar Micrometer Eyepiece Revolving Nosepieces Eyepieces Revolving Nosepieces for BF Objective Lenses Eyepieces for UIS2 optical system. Choose from following 6 types. For motorized nosepieces, refer to motorized unit page. Insert the DIC dummy when not using the DIC slider. WHN10x-H CROSSWHN10x Widefield Eyepieces WH15x Widefield Eyepiece SWH10x-H MICROSWH10x CROSSSWH10x Super Widefield Eyepieces U-5RE-2 Quintuple Revolving Nosepiece U-D6RE Sextuple Revolving Nosepiece with Slider Slot for DIC U-D6RE-ESD Sextuple Revolving Nosepiece with Slider Slot for DIC with ESD Treatment ø46.2 ø43.2 ø39 ø41 ø36.5 EP 104 83 36.8 53.2 18.7 29.6 48.6 27.8 (28.5) ø30 ø30 (87.4) 26.5 40 with Adjustable Diopter with Cross Lines and Adjustable Diopter 47.2 22 22 22 14 26.5 26.5 26.5 87.6 76.4 Remarks 48.2 WHN10x WHN10x-H CROSSWHN10x WH15x SWH10x-H MICROSWH10x CROSSSWH10x *EP=eyepoint 38 40 Field Number 38 (114.4) ø30 40.8 Name Diopter Weight Micrometer Adjustment Range Diameter (mm) (g) (1/m) — 24 90 -8 — +5 24 170 -8 — +5 — 170 — 24 90 -8 — +2 — 210 -8 — +2 — 210 -8 — +2 — 210 (125.6) 60.9 40 ø30 69.9 (23.2) (23.1) (25) 43.7 62.6 39.4 51.2 28 39.6 48.6 EP 60.2 ø41 ø38.5 41.4 ø41 ø38.5 U-D7RE Septuple Revolving Nosepiece with Slider Slot for DIC 116.5 WHN10x Widefield Eyepiece .4 ø102 with Adjustable Diopter with Micrometer and Adjustable Diopter with Cross Lines and Adjustable Diopter ø116.5 ø84 Unit: mm Weight: 520 g Weight: 800 g Weight: 980 g U-P4RE Centerable Quadruple Revolving Nosepiece with Slider Slot for DIC Filar Micrometer Eyepiece U-P6RE Centerable Sextuple Revolving Nosepiece with Slider Slot for DIC U-OSM Measuring Scale Scale Lines Graduated in Increments of 1 mm in the Entire 10 mm Length. Shift of Scale Lines: 1 mm per Rotation of the Sift Ring, the Circumference of Which is Divided into 100 Graduations. Measuring Range 10 mm/Objective Lens Magnification Mounting Position Inside Actual Size Repeatability 29.5 75.5 Accuracy Weight: 580 g Unit: mm 35 ±5 % by Combined Use of the Zoom Compensation Ring and the Provided Stage Micrometer. Compensation Ring Clamping screw. Magnification Compensation Scale. Actual Size (mm) = Measured Value (mm) Objective Lens Magnification 38 (125.6) (114.4) 76.4 38 0.007 mm A (A … Objective Lens Magnification) 87.6 40 Compensation Limit for Objective Lens Magnification Tolerance 47.2 EP 40 30 23.5 104 Magnification 10x, Erect Image (inverted when used with erect image observation tube), F.N. 14. Diopter Adjustment Range: ±5 1/m. Provided with Rubber Eye Shade. 48.2 60.9 65 41 137 129.2 117.8 Eyepiece 116.5 Used for precise measurement in the field of view. Repeatability Error ± *Measuring Error (A … Objective Lens Magnification: L … Measured Length in mm) 0.007 ± [ (0.0002 x A+0.002) L + ] mm A .4 ø102 .5 ø116 Weight: 1 kg Weight: 1 kg Unit: mm 36 Revolving Nosepieces Video Camera Adapters Revolving Nosepieces for BF/DF Objective Lenses C-mount Video Camera Ports Choose from following 3 types. Use of adapter to mount BF objectives (BD-M-AD) enables attachment of brightfield objective lenses. For motorized nosepieces, refer to motorized unit page. Allows direct attachment of a C-mount video camera. Four types are provided: 0.63x, 0.5x, 0.35x and 0.25x. All models feature a focus adjustment function. Insert the DIC dummy when not using the DIC slider. U-TV0.25xC C-mount Video Port with 0.25x Lens Image Plane 1-32UN Image Plane 12.4 22.4 147.3 156.84 104 1-32UN 116.5 2A00002 JAPAN U-D6BDRE U-D6BDRE 104 17.53 ø36 U-TV0.35xC-2 C-mount Video Port with 0.35x Lens 4 U-D6BDRE Sextuple Revolving Nosepiece for BF/DF with Slider Slot for DIC/ U-P5BDRE Centerable Quintuple Revolving Nosepiece 17.53 U-D5BDRE Quintuple Revolving Nosepiece for BF/DF with Slider Slot for DIC 3.5 U-5BDRE Quintuple Revolving Nosepiece for BF/DF N PA JA ø60 ø60 ø64 (125.6) 38 87.6 Weight: 1.2 kg 47.2 40 76.4 40 38 48.2 40 Weight: 100 g U-TV0.5xC-3 C-mount Video Port with 0.5x Lens U-TV0.63xC C-mount Video Port with 0.63x Lens .4 ø102 .4 ø102 .5 Ø116 ø30 1-32UN Weight: 1 kg 3.5 Weight: 800 g 1-32UN 68.75 78.25 3.5 ø30 30.1 32.6 42.1 17.53 Image Plane 2 A0 0 0 0 1 ø60 ø60 BD-M-AD Adapter to Mount BF Objectives Weight: 200 g Weight: 430 g W26 x 0.706 Unit: mm W20.32 x 0.706 Field of View (F.N.) 2/3" CCD +0.2 0 1/2" CCD Video Camera Adapter (projection lens) Weight: 10 g Projection Area (F.N.) Projection Magnifications 2/3" CCD 1/2" CCD 1x 11 8 6 0.63x 17.5 12.7 9.5 U-TV0.5xC-3 0.5x 22 16 12 U-TV0.35xC-2 0.35x — 22 17.1 U-TV0.25xC 0.25x — — 24 U-TV1x-2 U-TV0.63xC ø28.2 Weight: 800 g 17.53 Image Plane ø30 48.2 34.8 (114.4) (111.2) 76.4 Practical Field of View (mm) = 1/3" CCD Projection Area (field number) Objective Lens Magnifications Projection Area (4) 4 Unit: mm 8 37 Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching. Generally, the video camera adapter is focused by switching to a low magnification after focusing at a high magnification.objective lens. 38 Video Camera Adapters Motorized Units Video Camera Mount Adapters Motorized Units Allows attachment to video cameras with C, Bayonet, Sony and F mounts. Use with the U-TV1x-2. Focus by amount of screwing into U-TV1x-2. Various motorized units, perfect for automation of equipment, are available. U-CMAD3 C-mount Adapter U-BMAD Bayonet Mount Adapter U-SMAD Sony Mount Adapter BX-RLAA + U-D6REMC + U-LH100-3 Motorized BF/DF Reflected Light Illuminator + Motorized Nomarski DIC Sextuple Revolving Nosepiece + 100 W Halogen Lamp Housing Enables motorized exchange of objective lenses, selection between brightfield and darkfield observations as well as aperture diaphragm closing/opening. The BX-UCB control unit has an RS232C connector, allowing control via a PC. For method of attaching illuminator, refer to page 24. ø44.5 ø30 1-32UN Image Plane Image Plane 107 30 M56X2 40 60 3 80 ø48 115 108 M56X2 49 60.5 80.5 20 48.7 ø42 4 48 38 4 17.53 Image Plane M56X2 ø64 ø64 Weight: 165 g Weight: 80 g Weight: 90 g 11.8 12 ø64.4 86.2 (487) 87 U-TMAD T Mount Adapter 400 U-FMT F/T Mount Adapter * (169) 40 64.8 23 43 13 4 ø45.7 ø54.7 45 M56X2 81.5 M42X0.75 ø42 41 46.5 55 Image Plane 211.5 84 Image Plane ø64 Weight: 70 g Weight: 30 g Illuminator Cable Length: 1.800 mm * It must be combined with U-TMAD. Weight: 5.5 kg (exclude objective lens) Unit: mm BX-RFAA Motorized Universal Reflected Light Illuminator Reflected light fluorescence illuminator with simultaneous attachment of six mirror units. Incorporates motorized mirror unit changeover and shutter. Video Camera Port 17 12 76 U-TV1x-2 Video Port 1x 88 ø84 14 92 15 This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens. 11.6 27.2 Weight: 150 g Illuminator Mounting Position 87.5 Revolving Nosepiece Mounting Position 41 ø60 126 109.5 28 22 86.8 11.6 ø64 Illuminator Cable Length: 1.800 mm 135 261 Unit: mm 371 39 Weight: 4.3 kg 41 40 Unit: mm Motorized Units Motorized Units Motorized Units Various motorized units, perfect for automation of equipment, are available. U-D6BDREMC Motorized Sextuple BD Revolving Nosepiece with Slider Slot for DIC U-P5BDREMC Motorized Centerable Quintuple BD Revolving Nosepiece with Slider Slot for DIC BX-UCB Control Unit Motorized units including motorized illuminator and auto focus unit can be totally controlled from BX-UCB. U-HSTR2 Hand Switch 34 105 212 216 108 115.5 46 7° 146 83.5 103.5 135 Cable Length 2000 mm Weight: 370 g 40 69.8 51.1 40.5 25.5 150 125 310 332 Depth Weight: 1.0 kg Weight: 1.4 kg * Extension cord U-RMT (1700 mm) should be used to connect the lamp housing (U-LH100-3) to the BX-UCB. U-D5BDREMC Motorized Quintuple BD Revolving Nosepiece with Slider Slot for DIC U-D6REMC Motorized Sextuple Revolving Nosepiece with Slider Slot for DIC U-P5REMC* Motorized Centerable Quintuple Revolving Nosepiece with Slider Slot for DIC 34 14 50 46 115 BX-REMCB Control Box for Motorized Nosepiece and BF/DF Illuminator BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be controlled from U-HSTR2, or direct from the computer keyboard via an RS232C connector. * BX-RFAA and U-D5BDREM/U-D6REM combination not applicable. U-ACAD4515 AC Adapter for BX-REMCB 2000 +100 0 39.8 34 190.4 74.5 135 150 71±1 103.5 40 129.5±1 35±1 50 25.5 144 40.5 68.3 50 14 32 Unit: mm Unit: mm *Please contact Olympus for the availability. Weight: 1.1 kg 41 42 Motorized Units Motorized Units Motorized Units Various motorized units, perfect for automation of equipment, are available. U-AFA2M-VIS Active Auto Focus Unit Featuring an AF laser light source in wavelength 785 nm. The multiple-spots sensor enables the high-speed and stable focusing of specimens with variable height differences. 310.5 108 206 77 313 BXFMA-F Motorized Illumination with Power Focus A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens switching, aperture diaphragm open/close, and brightfield/darkfield switching are accomplished with this component. Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit. This is the configuration combined with Active Auto Focus Unit U-AFA2M-VIS, Single Port Tube with Lens U-TLU, a lamp housing, a motorized nosepiece and objective lenses. 45 133 45 Weight: 2.6 kg 32.5 169 Length: 2 m Length: 3 m * Consult your Olympus dealer about the motorized focus. 36 Weight: 0.23 kg Weight: 0.36 kg Stroke Specimen Surface (standard focusing position) 2 51 7 77 334 342.1 92 U-FWR Motorized Reflected Filter Wheel Accomplish maximum 6 filter position exchange. Weight: 13 kg (BXFMA-F frame 7.6 kg) * Consult your Olympus dealer about the mounting dimensions. 180.5 147.9 130 U-FH Focus Adjustment Knob Unit U-IFFH Focus Adjustment Knob Interface 70 Weight: 1.0 kg 100 104 3 50 24.5 58.5 91.5 33.6 42 Cable AFA2M-CBL2M AFA2M-CBL3M 30.5 51 56 62.5 2000 70 75.5 4 54 82.3 Weight: 760 g 210 Unit: mm 43 Weight: 1450 g 214 Unit: mm 44 Deep Ultraviolet Observation System Optical Terminology Deep Ultraviolet Observation System This module adds a deep ultraviolet (248 nm) optical system to a general microscope. An ultra-high resolution observation is executed by using an extremely short wavelength ray. ø8 Light Guide U-UVF248IM UV248 Compatible Intermediate Tube U-UVF2FB/5FB UV Quartz Light Guide 1. Field Number (F.N.) and Practical Field of View 5. Total Magnification The field number (F.N.) is referred to as the diaphragm size of eyepiece in mm unit which defines the image area of specimen. The diaphragm diameter actually seen through eyepiece is known as the practical field of view (F.O.V.) which is determined by the formula: 5.1 Observation Through Eyepiece (binocular observation) F.O.V. = M(bino)=M(ob)×M(oc) M(bino): Total magnification for binocular observation M(ob): Objective lens magnification M(oc): Eyepiece magnification Eyepiece F.N. (mm) Objective Lens Magnification 5.2 Video Monitor Observation ● Total Magnification for Video Monitor 79 2000 +200 0 or 5000 M(video monitor)=M(ob)×M(video camera adapter)×Monitor Magnification* +200 0 M(video monitor): Total Magnification on the Video Monitor M(ob): Objective Lens Magnification M(video camera adapter): Projected Magnification for Video Camera 69 30.4 108 34 2. Working Distance (W.D.) Weight: U-UVF2FB 50 g U-UVF5FB 80 g 105.8 258.5 The distance between the front edge of the objective lens and the specimen surface (with the surface of the cover glass in case of the cover glass objective lens) when the specimen is focused. Adapter Including Photo Eyepiece (refer to Figure 1) * Refer to Figure 3 for "Monitor Magnification" ø39.4 ø30 1-32 UNF C Mount Thread ● Practical Field of View for Video Monitor Observation 20 (39.5) 5.5 Working Distance and Parfocal Distance 196 (refer to Figure 1 for projected magnifications) * Refer to Figure 2 for Image Device Size Figure 1 Video Camera Adapter and Projection Magnifications Video Camera Adapter (projection lens) U-TV1x-1 + Video Camera Mount Adapters Parfocal Distance 45 M(ob)×M(video camera adapter) Adapter Including Photo Eyepiece Objective Lens Mounting Position U-UVF248LB+U-LH80HGXE UV248 Compatible Light Source Box + Mercury Xenon Lamp Housing 30 Image Device Size * M(ob): Objective Lens Magnification M(video camera adapter): Projected Magnification for Video Camera Weight: 1.9 kg 6 Working Distance (W.D.) 150 Projection Magnifications 1x U-TV0.63xC U-TV0.5xC-3 U-TV0.35xC-2 U-TV0.25xC 180 0.63x 0.5x 0.35x 0.25x Figure 2 Imaging Device Size 210.3 150 170 Focal Plane Camera Format 1/3" 1/2" 2/3" For parfocal distance of the LCPLFLN-LCD series objective lenses, refer to the appropriate objective lens page. Diagonal 6.0 mm 8.0 mm 11.0 mm Horizontal 4.8 mm 6.4 mm 8.8 mm Vertical 3.6 mm 4.8 mm 6.6 mm The above table is for standard image device sizes. Check your device size for precise calculation. 4-ø4.5, ø8 C'bore 5 Deep 4-ø4.5, ø13 C'bore 5 Deep 4. Relationship Between the Objective Lens's Focal Length and Magnifications 8 Figure 3 Imaging Device Size and Monitor Magnifications Monitor Size (diagonal) Camera Format 10" 15" 17" 19" 21" 1/3" 42.3x 63.5x 72.0x 80.4x 88.9x 1/2" 31.8x 47.6x 54.0x 60.3x 66.7x 2/3" 23.1x 34.6x 39.3x 43.9x 48.5x 227 Indicated magnifications of UIS2/UIS objective lenses are the values when the focal length of the tube lens is 180 mm. 170 45 Distance Between Mounting Positions 196.8 ø70 Practical Field of View for = Video Monitor Observation It is the distance between the objective lens mounting plane and the specimen. In UIS2/UIS objective lenses, the parfocal distance is designed at 45 mm. 157.5 102.5 3. Parfocal Distance M(ob)= Focal Length of Tube Lens f M(ob): Objective Lens Magnification f: Objective Lens's Focal Length 240 45 41 Weight: 6.5 kg Unit: mm 46 OPTICAL TERMINOLOGY Example What is total magnifications for video monitor when objective lens is 50x, video camera adapter U-TV0.5xC, 2/3" video camera and 21" monitor are used ? 7. Resolving Power The resolving power of an objective lens is measured by its ability to differentiate two lines or points in an object. The greater the resolving power, the smaller the minimum distance between two lines or points that can still be distinguished. The larger the N.A., the higher the resolving power. •Total magnification on the video monitor: m(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and monitor magnification is 48.5× from Figure 3. M(monitor observation)=M(ob)×M(video camera adapter)×monitor magnification =50×0.5×48.5=1213× ● Resolving Power Formula The following formula is generally used for determing resolution. λ (Reyleigh formula) ε = 0.61 × N.A. λ: Wavelength or Radiation in Use (λ=0.55 µm is used for visible light.) N.A.: Objective Lens N.A. •Practical filed of view for video observation (horizontal side): M(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and horizontal side of 2/3" imaging device is 8.8mm from Figure 2 Practical Field of View = for Video Observation Image Device Size M(ob) × M(video camera adapter) Example MPLFLN100×(N.A.=0.90), λ=0.55 µm 8.8 (mm) = =352 µm 50 × 0.5 ε = 0.61 × λ 0.3355 0.3355 = = = 0.37 µm N.A. N.A. 0.90 6. Numerical Aperture (N.A.) The numerical aperture is a key factor to the performance of objective lens (resolving power, focal depth and brightness). The N.A. is determined by the following formula: 8. Focal Depth of Microscope The focal depth refers to the depth of the specimen layer which is in sharp focus at the same time, even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope. As human eyes are individually different in the ability of their focus adjustment, each person's perception of the focal depth varies. At present, the Berek formula is generally used, because it gives a focal depth value that often coincides with that obtained through experiments. n=Refraction Rate of the Medium between Specimen and Objective Lenses. (Air: n=1, oil: n=1.515) θ: Angle Which is Made by the Optical Axis and Refraction of the Light Farthest from the Center of the Lens. The visual field brightness (B) of the microscope is determined by the following formula in relation to the objective lens magnification (M). The larger the N.A. and the lower the objective magnification, brightness will increase in the factor of the second power. Focal Depth formula ● Visual Observation (Berek formula) N.A.2 M2 ± D.O.F.= ω × 250,000 λ (µm) + N.A. × M 2 (N.A.) 2 D.O.F.: Depth Of Focus ω: Resolving Power of Eyes 0.0014 (when optical angle is 0.5 degrees) M: Total Magnification (objective lens magnification x eyepiece magnification) Numerical Aperture Objective Lens n=1 (air) ● Video Camera In the case of a video camera, the focal depth will vary according to number of pixels of CCD, optical magnification, and numerical aperture. The above-mentioned formula is used as a rough guide only. Types (1) to (3) correspond to “expansion of a point image” that goes against requirement (i) for ideal image formation in Figure 9-1. Type (4) corresponds to “curvature of image plane” that goes against requirement (ii) in Figure 9-1. Type (5) corresponds to “deformation” that goes against requirement (iii) in Figure 9-1. Types (6) and (7) correspond to “color blur” of images caused by characteristics of glass materials used for the optical system. “Expansion of a point image” can also be expressed by “wavefront aberration” that regards the light as “waves” and takes account of the phase to include the influence of diffraction. 9. Aberrations A difference between an ideal image and an actual image that passes through an optical system is called an “aberration.” (1) Spherical Aberration When light rays coming out of an axial object point enter a lens, the light rays with a larger numerical aperture (N.A.) are subjected to stronger refraction power and cross the optical axis in positions with larger differences from the ideal image formation position. The aberration caused this way by different image forming positions due to differences in N.A. of axial light rays is called “spherical aberration.” (“Spherical aberration” is proportional to the cube of N.A.) 9.1 Requirements for Ideal Image Formation The following three requirements must be satisfied to form an image with no aberration, or an ideal image. (i) All the light rays coming from a single point and passing through an image formation optical system converge on a single point. (ii) Image points, which correspond to object points on the same plane perpendicular to the optical axis, are present on the same plane. (iii) The planar shape of an object and the planar shape of an image that are on the same plane perpendicular to the optical axis have a similarity relation. Figure 9-3 Specimen N.A.= n × sinθ B∝ OPTICAL TERMINOLOGY Figure 9-1 Object （i） Image Plane （ii） （iii） Objective Lens with Spherical Aberration Inner Light Ray (with a smaller N.A.) In an actual optical system, however, it is very difficult to strictly meet the requirements for ideal image formation and this causes “aberrations” that interfere with image forming performance. Figure 9-2 Figure 9-4 (2) Coma Aberration Seidel's Aberration Coma Aberration and Spot Shape on the Image Plane (3) Astigmatism Specimen (4) Field Curvature Aplanatic Tube Lens (5) Distortion Aberration 47 Outer Light Ray Focal Position (2) Coma Aberration Even though spherical aberration is compensated to be very small, there are cases where light rays coming out of an off-axis object point are not condensed to a single point on the image plane but generate asymmetric blur just like a comet leaving traces. This is called coma aberration. Classification of Aberrations This indicates that the focal depth becomes smaller as the numerical aperture becomes larger. ± D.O.F. = Inner Light Ray Focal Position It is said that objective lenses with larger N.A. have better resolution but worsen spherical aberration. Our advanced design and manufacturing techniques have realized good optical performance even with large numerical aperture. 9.2 Classification of Aberrations Aberrations that interfere with image forming performance are classified as shown below in Figure 9-2. Seidel’s Aberration = “Expansion of a Point Image” + “Curvature of Image Plane” + “Deformation” 350 0.275 + (λ=0.55 µm) N.A. × M N.A.2 Example With MPLFLN100× (N.A.=0.90), WHN10×: Image Plane Outer Light Ray (with a larger N.A.) (1) Spherical Aberration Sample Surface Aplanatic Tube Lens Requirements for Ideal Image Formation θ ➔ ± D.O.F. = Spherical Aberration Chromatic Aberration (6) Longitudinal (axial) Chromatic Aberration Objective Lens with Coma Aberration (7) Chromatic Aberration of Magnification 350 0.275 + = 0.39 + 0.34 = 0.73 µm 0.90 × 1,000 0.81 48 Image Plane Optical Terminology (3) Astigmatism Even though a lens is compensated for spherical aberration and coma aberration, there are cases where an image of an off-axis object point is not focused to a single point but separated to a concentric line image and a radial line image. This is called “astigmatism.” When astigmatism is present, a point image blurs vertically and horizontally, before and after the focus position. Figure 9-5 (6) Chromatic Aberration Glasses used for optical systems have different refractive indexes depending on the wavelength. This causes differences in focal length between wavelengths and generates displacement of image forming position. This phenomenon is called “chromatic aberration,” which is sometimes subdivided into axial displacement on the optical axis, called “axial chromatic aberration” (or lateral chromatic aberration) and displacement on the image plane, called “chromatic aberration of magnitude.” Many special glass materials are used, e.g., for apochromats, to eliminate chromatic aberration in a wide range from violet light (g-rays with wavelength of 435 nm) to red light (c-rays with wavelength of 656 nm). Astigmatism and Change in Spot Shape in Different Focus Positions Optical Terminology 9.4 Strehl Ratio When a point light source is observed with an aberration-free optical system and an aberrated optical system , the former concentrates the focal point to a point at the image formation position. In contrast, the latter fails to produce a focal point, instead causing a spread in the intensity distribution of the point image (this is known as “point spread”). The specific appearance of such a point image (i.e. point spread) is shown in Figure 9-9. 9.3 Wavefront Aberration Since a long time ago, aberrations have been used in “geometric optics,” which considers light as “light rays.” Microscope optical systems are often used for observation of very small specimens at a wavelength level, and sometimes adopt “wave optics,” which regards light as “waves” and handles the phase information, taking account of the influence of diffraction. In such a case, “wavefront aberration” is used for evaluation. As shown below, when requirements for ideal imaging are satisfied in a microscope optical system, the spherical wavefront (spherical waves) coming from a single point on an object (specimen) is converted to plane waves through an ideal objective lens. The plane waves are converted to spherical waves through an ideal tube lens, and condensed to a single point. The wavefront of these waves is called the “ideal wavefront.” Figure 9-9 Appearance of Condensed Light in the Image Plane (point spread) (a) (b) (c) Figure 9-7 (a) (b) Ideal Microscope Optical System (c) Specimen Ideal Objective Lens (4) Field Curvature An image plane of an object on a plane perpendicular to an optical axis does not always become a plane perpendicular to the optical axis, but it generally becomes a curved plane. This symptom is called “field curvature.” When field curvature is present, the image is more displaced as it becomes closer to the periphery of the visual field. Therefore, when the center of an image is brought into focus, blur occurs in the peripheral areas of the image. To bring the entire image, including the periphery, into clear focus, it is necessary to adequately compensate for this type of aberration. Spherical Wave Ideal Tube Lens Plane Wave Image Plane Aberration-free Optical System With the proportion of light concentrated in the image plane (intensity of light concentrated in the Airy disk) by an aberrationfree optical system serving as 100%, the proportion of light concentrated by an aberrated optical system is known as the Strehl ratio. When graphed, the Strehl ratio reveals peaks in intensity as shown in Figure 9-10. The higher the S.R., the closer an optical system is to being aberration-free. Spherical Wave Based on the figure indicated for (1) spherical aberration, the behavior of the wavefront in an optical system that has an aberration is described below. Figure 9-8 Figure 9-10 Illustration of Wavefront Aberration Specimen Aberration-free Optical System Ideal Wavefront 100% Aberrated Optical System Objective Lens with Spherical Aberration Figure 9-6 Strehl Ratio (S.R.) Intensity Actual Wavefront (5) Distortion When there is no similar relation between a planar shape on an object and a shape on the image plane, this is called “distortion.” When distortion is present, a square image appears in a shape of a barrel or pin-cushion as shown in Figure 9-6. Aberrated Optical System S.R. Distortion (a) Barrel Shape Type A difference (a degree of disagreement) between the ideal wavefront and the actual wavefront shown above is called “wavefront aberration.” (a) Pin-cushion Type Image Plane A Strehl ratio of 80% is typically called the diffraction limit, and lenses with a lower ratio lack the performance required to serve as an objective lens. A ratio of over 95% means that the lens’ performance in general observations is comparable to that of an aplanatic lens (which is corrected for spherical aberrations and coma). Note: A laser interferometer is used for actual assessment of optical performance, so assessment is done at a single wavelength. Unless otherwise noted, Strehl ratio measurements are at the e-line (544 nm). The microscope optical system contains some distortion. When distortion is present, it can bring erroneous results of shape measurements. When a microscope is used for precision measurements, pay close attention to this aberration, for example, by providing it with an aberration compensation function. 49 50