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MOTICAMPRO P R O F E S S I O N A L C C D M I C R O S C O P Y C A M E R A S 2 MOTICAMPRO The Moticam PRO series contains 12 models with different SONY ICX sensor resolutions and technical characteristics, providing users with a wide variety of options to choose from. It includes Colour CCD imaging sensors for conventional microscopy techniques, Monochrome CCD imaging sensors for low luminosity microscopy and Peltier cooled CCD Colour and Monochrome imaging sensors for fluorescence microscopy. The cameras are assembled and tested to the highest standards in the clean room at our factory. All Moticam PRO cameras come with the universal c-mount thread and are connected to the PC via a USB 2.0 port. The Moticam PRO Series comes with the well known and user-friendly Motic Images Plus 2.0 analyzing software (multilanguage). Additionally with any Moticam PRO, the sophisticated Motic Images Advanced 3.2 is supplied free of charge. Furthermore the Motic TWAIN drivers, Direct Show drivers (via our website) and the SDK are included with the software package of the Moticam PRO. 3 Peltier Cooling device When using a camera for a long time, the sensor gets warmer and warmer. When using long exposure times, it becomes more sensitive to noise and this can be seen in the captured image. With our Peltier mechanism, the sensor is cooled down up to 10°C below ambient temperature (ambient = the temperature inside the camera case). Peltier Cooled cameras are therefore recommended for fluorescence applications. Live readout The cooled version of the Moticam PRO features a built-in sensor arrays that allows the user to see live updated information on the Sensor and Ambient Temperature, as well as the Relative Humidity inside the casing. Thus eliminating the possibility of condensation brought on by rapid cooling. Additionally the user can set a “Target Temperature”. Colour and Monochrome Colour cameras use a RGB primary colour mosaic filter on the chip. The distribution is standard; 25% Red, 50% Green and 25% Blue. Most of the time when working in fluorescence only one colour is revealed; therefore not allowing all available pixels to receive information. Just imagine seeing a blue image through the eyepieces, this means that only 25% of the total pixels are receiving and distributing information. The Monochrome cameras do not have any colour filter and therefore each pixel records the amount of light it sees. It does not transmit any information on the colour of the sample to the computer. For these reasons monochrome cameras can definitely be considered when working with fluorescence. You will have better chances of capturing low light samples when using the full capacity of the pixels in the chip. Of course colour is important in fluorescence, but this can be added at a later stage with software. 4 MODEL SENSOR SIZE PIXEL SIZE RESOLUTION 1/2” 4,65 x 4,65 1360 x 1024 ICX252AQ 1/1.8” 3,45 x 3,45 2080 x 1542 ICX282AQ 2/3” 3,40 x 3,40 2580 x 1944 2/3” 6,45 x 6,45 1360 x 1024 SONY SENSOR A ICX205AK MOTICAMPRO 205 B C ICX205AL D MOTICAMPRO 252 MOTICAMPRO 282 A B A B A ICX285AQ MOTICAMPRO 285 B C ICX285AL D NOMENCLATURE Monochrome Colour Peltier cooled High speed 5 Motic Images Plus 3.2 This software contains all functions of our well-known Motic Images Plus 2.0 software plus much more. Its main features are: Motic Images Advanced 3.2 - Counting Capture Auto Capture Video recording Measurements Automated counting Creating reports Image comparison Amalgamation What makes Motic Images Advanced 3.2 software a more sophisticated version are the following features: segmentation, multi-focus and assembly. Segmentation Motic Images Advanced 3.2 - Assembly The superior counting module allows you to perform manual segmentations by using a histogram (colour), grey scale or by selecting the size. You can create in one sample different groups, which will be highlighted in different colours. After the segmentation has been performed, you will have information about the area in square µm, perimeter, width, height and much more details. The results of the segmentation can be exported in an excel file for further analysis. Assembly This module can be used for creating a single overview image out of multiple separate images. The software recognizes any overlapping and corrects this automatically. This software is specially designed for users who wish to have an overview of the complete sample, while working at a higher magnification. Motic Images Advanced 3.2 - Segmentation Multi Focus The multi-focus module can be used to capture up to 100 images taken at different focusing levels. The software will automatically recognize the parts that are in focus in each image and assemble them into a new file. The result is a single image that is completely in focus. * Motic Images Advanced 3.2 is available in English language and is only compatible with PC. Motic Images Advanced 3.2 - Selection 6 Hardware s3ONY)#8##$IMAGINGSENSOR s-OTICCONTROL PROCESSINGIMAGINGBOARDS s"UILT INFRAMEBUFFER s"UILT IN&0'!PROCESSOR s44,TRIGGERPORT s3CHOTT"' FILTER s#3#MOUNTC RINGPROVIDED s53"DATAMINIPORTUSBCABLEPROVIDED s5NIVERSALPOWERSUPPLYFORCOOLEDCAMERASONLY s0ELTIERCOOLINGDEVICEFORCOOLEDCAMERASONLY s#OOLINGUPTODEGREESBELOWAMBIENTWITHTEMPERATUREREADOUT * Unlockable with SDK integration Software s-OTIC)MAGES0LUSAPPLICATIONSOFTWARE s-OTIC)MAGES!DVANCEDAPPLICATIONSOFTWAREFOR7INDOWSCOMPUTERONLY s4WAINDRIVERS s-OTIC3$+ s-OTIC$IRECT3HOWDRIVERS s-OTIC-)$EVICESLIVEIMAGINGMODULEWITHLIVECALIBRATIONGRID SCALECROSSANDSCALEBARS * Available through free download Minimum computer specifications s)NTEL'(: s'"2!s53" s7INDOWS8030 s-ACINTOSH/38 7 Quantum Efficiency Diagrams Moticam Pro 205A Moticam Pro 205C & 205B G 0.8 R 0.8 B 0.6 Relative response Relative response 0.6 0.4 0.2 0 400 500 600 0.4 0.2 0 700 400 Wave length 1.0 500 B & 252B B 900 1000 0.6 0.6 0.4 0.2 0 500 650 700 900 1000 & 282B 550 G R 0.8 Relative response Relative response R 600 650 0.4 0.2 0 700 400 Wave length 450 500 550 600 Wave length Moticam Pro 285A & 285B Moticam Pro 285C 1.0 & 285D 1.0 0.9 R G 0.9 0.8 0.8 0.7 0.7 B 0.6 Relative response 0.6 Relative response 800 1.0 G 450 700 Moticam Pro 282A 0.8 400 600 Wave length Moticam Pro 252A 0.5 0.4 0.3 0.2 0.1 0 400 450 Wave length 8 &205D 1.0 1.0 500 550 600 650 700 0.5 0.4 0.3 0.2 0.1 0 400 500 Wave length 600 700 800 Unit Control Software (MI Device) Available options Video Device Connection of multiple devices at the same time (Selection of the active device) ROI function Region Object Interest (manual selection) Exposure time Manual / Automatic Full screen Automatic White Balace Set Method, color balance ajustment Image adjustments Gain / Offset / Enhance / Gamma Storage Format SFC / JPG / BMP / TIFF / DCM (Available on Motic Advanced 3.2) Background Balance Manual Color correction +/- 10 (RGB) Histogram Live window in real time Filters Live Image Invert / Gray / Emboss / Red / Green / Blue / Red reverse / Green reverse / Blue reverse Edge detection 0 / 10 Sharpness +/- 10 Remove Noise 4 Levels Scale Live Images, Horizontal and Vertical (user configurable) Grid User configurable Scale Cross X e Y distances (select position) Record parameters Single capture / Auto capture / Video Capture Cooled Enable Only avalaible with cooled cameras Cooled Cameras Internal Sensor with target window (Sensor temp / Ambient temp / Ambient humidity / Dow point) 9 Moticam Pro Moticam Pro 205A 205A 252A 282A 252A 282A 285A 285A 205B 252B 205B CCD Sony ICX CCD Sony ICX 1/2” 1/2” 1/1.8” 1/1.8” 2/3” 2/3” 2/3” 2/3” 1/2” 1/2” 1/1.8” Features Features Resolution Resolution Total pixels Total pixels 1392x1040 1.4 Mp Number of active pixels Number of active 1360x1024 pixels Pixel Size (microns) Pixel Size (microns) 4.65x4.65 Live display mode and capture size Live display1360x1024 mode and capture size 680x512 320x256 Select Size (ROI) Scan Scan Progressive Maximun Frame Rate* Maximun Frame 10 frames/S Rate Buffer Buffer Integrated Filter Integrated Filter Bit Depth Bit Depth Shutter Shutter Lens mount Lens mount Exposure Time Exposure Time Data Transfer Data Transfer Power Supply Power Supply Peltier Device Peltier Device 1.4Mp 3.2 Mp 3.25.0 MpMp 5.0Mp 1.4 Mp 1.41.4 MpMp 1.4Mp 3.2 Mp 1392x1040 2088x1550 2088x1550 2588x1960 2588X1960 1392x1040 1392x1040 1392x1040 1392x1040 2088x1550 2080x1542 1360x1024 (4:3) 2080x1542 2580x1944 (4:3) 1360x1024 2580x1944 (4:3) 1360x1024 1360x1024 (4:3) 2080x1542 1360x1024 (4:3) 2 4.65x4.65 3.45x3.45 3.45x3.45 3.40x3.40 3.40x3.40 6.45x6.45 6.45x6.45 4.65x4.65 4.65x4.65 3.45x3.45 1360x1024 2048x1536 680x512 1024x768 320x256 512x384 Select Select Size Size (ROI) (ROI) 2048x1536 2560x1920 1024x768 1280x960 512x384 640x480 Select Select Size Size (ROI) (ROI) 2560x1920 1360x1024 1280x960 680x512 640x480 340x256 Select Select Size Size (ROI) (ROI) 1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI) 1360x1024 2048x1536 680x512 1024x768 320x256 512x384 Select Select Size Size (ROI) (ROI) Progressive Frame Readout 108.5 frames/S frames/S Frame Readout 8.57frames/S frames/S Progressive Progressive 7 frames/S 15 frames/S 4 Frames 1510 frames/S frames/S 108.5 frames/S frames/S 4 Frames 8 bits / 12 Bits (Switchable 8 bits / 12 through Bits (Switchable MI Device, usable throughwith MI Device, application usable software with application that supports software 12 Bit) that supports 12 Continuous Variable-Speed Continuous Variable-Speed C-Mount C-Mount 1/1000 to 6 sec 1/1000 to 6 sec Mini USB2.0 connection mini USB2.0 connection 5V (USB) Universal Power Supply Universal (5V) Power S 5V (USB) Up to 10º Celsius below Up toambient 10º Celsius b 0 ~ 60º C 0 ~ 60º C 40% - 80% (35º) 40% - 80% (35º) Operating Humidity Camera Data Readout Camera Data Readout Sensor Temperature,Sensor Ambient Temperature, Temperature, Ambient Relative Temperature, Humidity, Dew Relative PointHumidity, Dew Point 117mm (L) x 65mm117mm (W) x 62mm (L) x 65mm (H) (W) x 62mm (H) Dimensions Dimensions Weight Weight Support Device Support Device Driver USB2 DeviceDriver / TWAIN USB2 / SDK Device / Direct / TWAIN Show/ Driver SDK / /Direct TriggerShow Port Driver (TTL) / Trigger Port (TTL) OS Compatibility OS Compatibility Windows XP / Vista /Windows Seven and XP MAC / Vista OSX / Seven and MAC OSX Software Suite Software Suite *under optimal illumination conditions 10 Frame Schott BG 40 Bandpass Schott BG 40 Bandpass Operating Temperature Operating Temperature Operating Humidity Colour Colour Colour 406 gr 406 gr 546 gr 546 g Motic Images Plus 2.0 Motic (PCImages & MAC), Plus Motic 2.0 Images (PC & MAC), Advanced Motic3.2 Images (PC), Advanced TWAIN, SDK, 3.2 (PC), MoticTWAIN, Direct Show SDK,Drivers Motic Direct Show ) 282B 285B 285B 205C 2/3” 2/3” 2/3” 1/2” 285C 205D 285C 205C 1/2” 2/3” 2/3” 1/2” Monochrome Monochrome Peltier 285D 205D 285DPro Moticam Moticam Pro 1/2” 2/3” CCD2/3” Sony ICX CCD Sony ICX Peltier Monochrome Peltier MonochromeFeatures 5.0Mp 1.4 Mp 1.41.4Mp Mp 1.4Mp 1.4 Mp 1.41.4Mp Mp 1.4Mp 1.4 Mp 2588X1960 1392x1040 1392x1040 1392x1040 1392x1040 1392x1040 1392x1040 1392x1040 1392x1040 1392x1040 1360x1024 2580x1944 (4:3) 1360x1024 1360x1024 (4:3) 4.65x4.65 6.45x6.45 6.45x6.45 4.65x4.65 4.65x4.65 6.45x6.45 2560x1920 1360x1024 1280x960 680x512 640x480 340x256 Select Select Size Size (ROI) (ROI) 1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI) 1360x1024 1360x1024 680x512 680x512 320x256 340x256 Select Select Size Size (ROI) (ROI) 1360x1024 1360x1024 680x512 680x512 340x256 320x256 Select Select Size Size (ROI) (ROI) 1360x1024 1360x1024 680x512 680x512 320x256 340x256 Select Select Size Size (ROI) (ROI) 7 15 frames/S frames/S 1510 frames/S frames/S Progressive 1015 frames/S frames/S 4 Frames Resolution 1392x1040 Total pixels Total pixels Pixel 6.45x6.45 Size (microns) 6.45x6.45 4.65x4.65 Progressive Resolution 1.4 Mp 1360x1024 1360x1024 (4:3) 1360x1024 Number of active (4:3) pixels Number of active pixels 1360x1024 1360x1024 (4:3) 1360x1024 1360x1024 (4:3) 3.40x3.40 6.45x6.45 Readout 1360x1024 Live display mode and 680x512 capture size 340x256 Select Size (ROI) Scan 1510 frames/S frames/S 1015 frames/S frames/S 4 Frames Schott BG 40 Bandpass Schott BG 40 Bandpass Continuous Variable-Speed Continuous Variable-Speed C-Mount 1/1000 to 6 sec 1/1000 to 6 sec 5V (USB) 546 gr 0 ~ 60º C Buffer Buffer Integrated Filter Integrated Filter Lens mount Exposure Time Exposure Time Data Transfer Data Transfer Power(5V) Supply Universal Power Supply Universal (5V) Power Supply Power Supply Peltier Device Up to 10º Celsius below Up toambient 10º Celsius below ambient Peltier Device 0 ~ 60º C Operating Temperature Operating Temperature Operating Humidity Sensor Temperature,Sensor Ambient Temperature, Temperature, Ambient Relative Temperature, Humidity, Dew Relative PointHumidity, Dew Point 117mm (L) x 65mm117mm (W) x 62mm (L) x 65mm (H) (W) x 62mm (H) 406 gr Bit Depth Lens mount 40% - 80% (35º) 40% - 80% (35º) 546 gr Scan Shutter 5V (USB) elsius below Up toambient 10º Celsius below ambient Live display mode and capture size Shutter mini USB2.0 connection mini USB2.0 connection Power Supply Universal (5V) Power Supply (5V) Pixel Size (microns) 15 Maximun frames/S Frame Rate Maximun Frame Rate bits / 12 Bits (Switchable 8 bits / 12 through Bits (Switchable MI Device, usable throughwith MI Device, application usable software with application that supports software 12 Bit) that supports 12 Bit)Bit Depth C-Mount Features 406 gr Camera Data Readout Camera Data Readout Dimensions 546 gr Operating Humidity 546 gr Weight Dimensions Weight Driver USB2 DeviceDriver / TWAIN USB2 / SDK Device / Direct / TWAIN Show/ Driver SDK / /Direct TriggerShow Port Driver (TTL) / Trigger Port (TTL) Support Device Support Device Windows XP / Vista /Windows Seven and XP MAC / Vista OSX / Seven and MAC OSX OS Compatibility OS Compatibility mages Plus 2.0 Motic (PCImages & MAC), Plus Motic 2.0 Images (PC & MAC), Advanced Motic3.2 Images (PC), Advanced TWAIN, SDK, 3.2 (PC), MoticTWAIN, Direct Show SDK,Drivers Motic Direct Show Drivers Software Suite Software Suite 11 www.motic.com Canada | China | Germany | Spain | USA Motic is a certified ISO9001, ISO14001 and ISO13485 company. Designed in Barcelona (Spain) Motic Incorporation Limited Copyright © 2002-2012. All Rights Reserved. May 2012 DE | EN | ES | FR | IT | PT | RU