Nikon Imaging Centre Showcase

Transcript

Nikon Imaging Centre Showcase Global Nikon Imaging Centers NIC@KCL - Current Status  Site identified - 3rd Floor of Hodgkin Building, Guys Campus KCL: Guy’s Campus NIC@KCL     Architects designs completed - building work to commence Aug 2011 Planned date for opening to users - Jan 2012 Initial contract for 5 years with a view to extend to 10 years NIC@KCL Steering Committee formed - will be responsible for overseeing running of NIC NIC@KCL - Overview of Instruments      List of microscopes assembled following consultation with Nikon and identified core users 10 systems on initial list - has some flexibility before opening of NIC Once NIC@KCL is open, additional changes to systems can be made as and when required to ensure most appropriate/useful microscopes are in place at any one time All microscopes run on same Nikon software 6 additional ‘off-line’ work stations for image analysis purposes will also be housed in NIC Systems Overview Simon Denham Systems Overview Ten Systems     Four Confocal Systems Three Live Cell Wide Field Systems Two Super Resolution Systems Macro Microscope System Confocal Systems A1R Si     High resolution and high speed Inverted microscope live / fixed specimens Spectral un-mixing Use your favourite fluorophores Confocal Systems A1R MP  Multi-photon – for deep in vivo / tissue imaging  On inverted microscope  Deep imaging at video rate  Use your favourite fluorophores Confocal Systems Spinning Disk x2     Inverted and upright systems Fast, cell friendly imaging Wide range of objectives Use your favourite fluorophores Macro System Macro Zoom AZ100     Multiple magnifications with zoom From whole animal to single cell DIC with all magnifications Use your favourite fluorophores Wide Field Camera Systems Ti-E Inverted Microscope     Full motorisation 6D Automated time lapse imaging Environmental control Use your favourite fluorophores Super Resolution System N-SIM     Structured illumination Ti-E inverted microscope Live cell imaging 2x Resolution vs conventional optics Super Resolution System N-STORM      STochastic Optical Reconstruction Microscopy Ti-E inverted microscope Photo-switchable Dye Pairs Fixed cell / tissue imaging 10x Resolution vs conventional optics - 20-30nm Super Resolution System N-STORM █ Cy3 / Alexa 647: Clathrin █ Cy2 / Alexa 647: Microtubule BioStation BioStation IM      Microscope and incubator all in one Place samples and go Simple click and go time lapse Phase contrast and fluorescence Suitable for small scale screens NIC @ KCL Overview Maddy Parsons NIC@KCL - User Training and Access  NIC will be run by a full-time Centre Manager - will oversee user training and access, as well as assisting in experimental design and image analysis  Users must register with NIC@KCL manager first to assess microscope needs and training requirements - details will be circulated once NIC opens  Full training provided on all systems NIC@KCL - Costs  Cost associated with using each instrument in NIC@KCL to cover maintenance charges Current charges are:        Widefield time-lapse = £4/h Spinning disk = £13/h Confocals = £22/h Multiphoton = £25/h Super-resolution = £16/h Whole animal imaging = £5/h Biostation = £5/h NB: use for 8-24h will be charged at rate for 8h NIC@KCL - Other Benefits  Contract agreement between Nikon and KCL means any PI within KCL can purchase systems within the NIC for a discounted price. Discount gets greater over the 5-year period to account for depreciation  PI’s can use these pre-agreed costs on grants for equipment purchases  Any systems purchased and removed from NIC will be replaced with new versions NIC@KCL - Steering Committee Members       Maddy Parsons Chair: Randall Division, Guys Campus) Giovanna Lalli (Wolfson/CARD, Guys Campus) Juan Martin-Serrano (DIIID, Guys Campus) Jon Clarke (MRC Dev Neuro, Guys Campus) Alex Ivetic (Cardiovascular, Denmark Hill Campus) Klaus Suhling (Physics, Strand Campus)  Robert Forster  Simon Denham (Nikon Instruments Division) (Nikon Instruments Division) Thank You Any Questions?