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Office Of The Dean, College Of Agriculture, Gwalior

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OFFICE OF THE DEAN, COLLEGE OF AGRICULTURE, GWALIOR – 474002 No. IPRO/Dean/SS/3140 Dated : 06/02/14 CORRIGENDUM The last date of tender No. IPRO/Dean/SS/3006 floated on 17.01.2014 for purchase of equipments required in Biotechnology Center is extended upto 2:00 PM of 17.02.2014. DEAN College of Agriculture, Gwalior RAJMATA VIJAYARAJA SCINDIA KRISHI VISHWA VIDYALAYA College of Agriculture, Gwalior – 474002 (M.P.) No. IPRO/Dean/SS/3006 Date: 17.01.2014 TENDER INVITING NOTICE Sealed tenders are invited from reputated firms/companies for purches of following equipments required in Biotechnology Center on or before February 05th, 2014 at 13.00 hours.For the tender form terms and conditions and details information please visit our website www.rvskvv.net after 22nd Jan 2014: GC, HPLC, Deep freezer (-200C), Deep freezer (-800C),Ultra Water Purification – Double stage system, Mini Centrifuge, Centrifuge Multipurposetabletop refrigerated, thermal cycler (PCR), Gel documentation system, Hybridization oven cum shaker, Electrophoresis apparatus (H& V), Shaker, Incubator, Laminor flow chamber (Vertical), Binocular stereoscopic microscopes, binocular microscopes 100x Magnification, Gel-documentation system with UV/WL, trans-illuminator-Visualizer with projection, Liquid nitrogen vessel, Phase contrast microscope, Ultracentrifuge, Glass ware washing system, ELISA reader, Ice palleting unit. DEAN College of Agriculture, Gwalior Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya College of Agriculture Gwalior – 474002 Tender form (Fill separately for each item) Tender No. Date of publication Last date of accepting tender Date of issuing office Amount of fee deposited for issuing tender form Rs. 1000/- Details of money receipt/DD No. & Date No. ………..……………date……………….. Signature of Person Issuing tender DETAIL OF TENDER FORM Item Quoted Name and Address of the agency quoting the rates Registered address of the company /firm Name of Proprietor with telephone no. Details of earnest money (DD/ Bankers cheque) Rs. Period for which DD/ Bankers cheque is issued Name of Bank issuing DD/Bankers Cheque No…………………………………………….. Date…………………………………………... RAJMATA VIJAYARAJE SCINDIA KRISHI VISHWA VIDYALAYA, COLLEGE OF AGRICULTURE, GWALIOR – 474 002 TERMS AND CONDITIONS Tender No. Date of Publication 17.01.2014 Date of issuing the tender form 27.01.2014 Time 10.00 to 17.00 (working hours) Last date for submission of the 17.02.2014 Time 14.00 hrs. at Office of Dean tender College of Agriculture, Gwalior Date and Time of opening of 17.02.2014 Time 15:00 hrs. at Office of Dean, Tender College of Agriculture, Gwalior 1. Only the manufacturer and their authorized dealer will submit the tender. 2. No person or firm shall submit more than one tender for the same item. 3. Broad based specification of the system / equipment is given along with the tender form. The tenderer should take note of it and if there are additional specification, which needs attention, should be mentioned in the specification. 4. The tenderer must submit the tender form issued by the office in original, tender document, signed copy of the terms and conditions duly stamped along with requisite EMD in a sealed cover, in the Office of the Dean, College of Agriculture, Gwalior 474002 by registered or speed post / courier or delivered up to 13.00 hrs of 05.02.2014 from the date of Publication. 5. Delayed tenders due to postal or any other reason will not be considered. 6. The EMD (Earnest Money Deposit 3% of price of the equipment quoted) valid for six months from the date of issue should be in the form of A/C Payee DD / Banker’s Cheque/ Fixed Deposit Receipt of a nationalized / Schedule Bank in favour of “Dean, College of Agriculture, Gwalior” payable at Gwalior. In absence of EMD with the prescribed form, the tender will not be considered. The EMD will be forfeited in case the tenderer fail to execute the order in specified time. 7. The tender offer should have validity for the period of 180 days. 8. The rates quoted should be inclusive of Sales Tax, F.O.R., VAT tax CST etc. College of Agriculture, Gwalior 474002, inclusive of packing & forwarding, loading, unloading and handling charges, freight, insurance, full risk coverage etc. The RVSKVV is exempted from Excise duty. 9. In case of imported item the CIF value should be Mumbai or Delhi Airport. The tenderer must specify the custom clearance and transportation charges. 10. Break of the prices for the main item and its accessories should be given separately. The tenderer should also specify the probable period of delivery of the equipment. 11. The tender will be opened on 17.02.2014 at 15.00 hrs by the members of the College level purchase committee in the Office of Dean, College of Agriculture, Gwalior 474002. The tenderer can depute his authorized representative to be present at the time of opening of the tenders. 12. The Dean, College of Agriculture, Gwalior reserves the right to extend the due date and the date of opening of the tender. 13. The EMD shall be released after 50 days from the date of opening of the tender. 14. The tenderer will arrange to supply the equipment as per the specifications within 10 days/ specified period of delivery from the date of issue of order failing which the order will be treated as cancelled. 15. The standard terms and conditions of payment acceptable or – payment in full will be made after the contract is effective in full and to the satisfaction of the authority. The payment will be released to the firm supplying the equipment only on successful installation. 16. Any deviation, variation or non-compliance by the tenderer shall be considered as breach of contract and 17. 18. 19. 20. 21. the undersigned reserves the right to forfeit the amount of the EMD in part or full and to take action as per legal arrangement. The Dean, College of Agriculture, Gwalior, reserves the right to accept or reject any or all the tenders without assigning the reasons thereof and to split of the tender as deemed fit. Should there arise any dispute, the Vice Chancellor, RVSKVV, Gwalior shall be the sole Arbitrator whose decision in the matter shall be final and binding. The tenderer shall have to give an undertaking that the terms and conditions as mentioned above are acceptable to the tenderer by providing a signed and dully stamped copy of the terms and conditions. Installation & essential training for operation of instrument will be duty of tenderer. The tender form worth Rs 1000/- can be purched either office of the Dean, College of Agriculture, Gwalior or downloaded from our website and submitted the same along with the DD of Rs 1000/- in favour of “Dean College of Agriculture” payable at Gwalior. Tenders are liable for rejection if:1. 2. 3. 4. Tender not received on due date and time. Earnest Money is not enclosed. The rates quoted are not F.O.R. Destination. The tender not submitted in the format supplied by the V.V. will not be considered and rejected at the time of opening. 5. Certificate of Registration for the type of Business is not enclosed with the tender. Dean College of Agriculture, Gwalior - 474002 Specifications of Gas Chromatograph The Gas Chromatograph System for Herbicides estimation should have the following specifications 1. GC must be Microprocessor based equipped with Electron Capture Detector (ECD) and automatic pneumatic control: 2. Column Oven: a) Oven should be large enough to accommodate two capillary columns b) Temperature Range: Room Temperature +10C to 400C or better c) Temperature Program Steps: 5 or more 3. Injector Split/Splitless Injection Unit 4. Detector Unit : ECD Electron Capture Detector (ECD): a) Dynamic range : More than 104 b) Minimum detection limit: 0.07pg -0.1pg or better c) Operating temp 350C or more in 1 °C increment 5. System should have built in diagnostics and comprehensive self test. 6. System should have large LCD graphic interphase for displaying all GC parameters including acquired chromatogram 7. Software: Windows based workstation software includes complete GC control, Real time chromatographic data acquisition and post‐run analysis. Software features include high speed data acquisition and bulk analysis compatibility, full qualitative & quantitative processing functions, multi‐function compatibility, GLP/GMP functions, Validation System Suitability, QA/QC functions, LAN Connectivity. 8. The offer should include one PC (with latest configuration) with b/w laserjet printer for operation and printing the spectra 9. Operational Accessories and Consumables for Gas Chromatography a) The offer should include five capillary columns: DB-5 30 m (length), 0.25 ID and 25 micron thickness or equivalent for Herbicide estimation( from OEM) b) Gas cylinders with regulators for H2, N2 and Zero air, Gas purification panel c) Ferrules (2 Pack) , syringes 10 µl (2), Septa (1 pack) and Liners (2) ( From OEM) 10. Online UPS 3KVA or appropriate capacity with 30 minutes back up time for instrumentPC-Printer. 11. Comprehensive Warranty for 3 Years. 12. AMC charges after the Warranty period should also be quoted for two year and the price validity should continue till termination of the period. 13. Installation and Demo should be provided by the vendor at no additional cost. 14. Training for operations and trouble shooting: at least 2 persons in our laboratory. 15. Please mention individual price of all items. Optional Split/Splitless Injection Unit (1No) Flame Ionization detector (FID): a) Temp. Range: Upto 400C in steps of 1 °C b) Detection Limit : 3 or better) pg C/sec c) Dynamic Range : More than 106 d) Flame out detection and Auto re-ignition Specifications for HPLC Microprocessor based latest Modular HPLC System with following Specifications is required along with columns suitable for herbicide/pesticides analysis 1. Binary Gradient with delivery system with built-in 3 Channel on line degasser .         Operating pressure: 6000 psi or better. Flow rate range : 0.01 to 10.00 ml/min Flow precision: ± 0.3% RSD Flow accuracy : +1% Flow calibration : Programmable The pump must be controlled from own keyboard and MS software Pump seal should be guaranteed for long run with any aqueous buffer, organic / non-polar solvents. 2. Manual Injector (Rheodyne) with built in 20ul loop with auto switch and gas tight 100ul syringe. 3. PDA Detector  Detection Type  Wavelength range  Wavelength accuracy  Sources      Photodiodes should be Linearity range Bandwidth Drift Noise : : : : : : : : : Single beam polychromator or better device. 200-700nm or more +/- 1nm min Simultaneous Deuterium lamp and Tungstenhalogen 512 or more 2AU with <5% deviation 2-170 or better 1.0 x 10-3AU/hr or better. < +/- 1 x 10-5 AU, 4. Operational all Accessories and Consumables for HPLC including columns (C 8 and C 18), Guard columns and other accessories for smooth operation of the system should be included ( from OEM). 5. Chromatography Software & Data Station: Single keyboard control of entire system Full 32 bit Architecture, Compatibility to import and export the data, Online help, GLP compliance, Flexible report publisher. 6. The offer should include one PC (with latest configuration) with b/w laserjet printer for operation and printing the spectra. 7. Online UPS of 3KVA or appropriate capacity with 30 minutes back up time for instrument-pc and Printer. 8. Comprehensive Warranty for 3 Years. AMC charges after the Warranty period should also be quoted. 9. AMC charges after the Warranty period should also be quoted for two year and the price validity should continue till termination of the period. 10. Installation and Demo should be provided by the vendor at no additional cost. 11. Training for operations and trouble shooting: at least 2 persons in our laboratory. 12. Please mention individual price of all items. Optional 13. Column Oven :  Temperature Range : 35 0C to 650C or better  Temperature Accuracy : + 10 C min  Should able to hold min. Minimum 2 column 14. Peltier Column Oven :  Temperature Range : 100C to 700C or better  Temperature Accuracy : + 10 C min  Should able to hold min. Minimum 2 column 15. Isocratic Pump system Specifications: 3. Binocular Compound Microscope Binocular with co – axial coarse & fine focusing supported on ball bearing .45 Degree inclined, 360 degree rotatable. Extra wide Field, Anti fungle, Anti reflected coated Eye Piece : WF 10x paired /20mm with diopter adjustment on both eyepieces & rubber eyecups Reversed Quadruple (4 objective) nosepiece. Plan Achromatic DIN SP Objectives 4x, 10x, 40x SL, 100x SL-Oil, Coaxial coarse and fine mechanism system with ball bearing guide ways . Built in low position coaxial mechanical stage. Focusable 1.25 NA Abbe condenser with Iris .Built in Halogen 6V/20W illumination with intensity control. Switching power supply 100-240V.Blue filter 45mm.Vinyl Dust Cover. 4. Binocular Stereoscopic Microscope. Zoom Stereo Microscope stereo with objective range of zoom magnification 0.7 to 4.5 zoom optical, with W.F.10x paired eye piece. Standard magnification is 7 x to 45x which is expandable with high powered eye piece & auxiliary objective lenses. Built-in powerful LED illumination for transmitted light and incident light microscopy 12V-20W complete with heat absorbing filter to Safeguard from heat. Stand fitted with easy - to - reach controls for focusing, zoom changer and illumination. 5. DEEP Freezer -20°c Double walled Microprocessor controlled with Automatic Voltage Stabilizer with thick PUF insulation between inner & outer wall. Hermatically Sealed Compressor with Castor wheels. Inner made of 304 grade stainless steel and outer of power coated MS with CFC free super dense PUF insulation.Horizontal Chest / vertical type models are supplied in a single compartment whereas and vertical models are supplied with SS.Storingshelves.Fitted with Digital Temperature Cum Controller . Ambient to - 20°C Capacity – 171/280/336ltrs. 6. Laminar Flow Chamber Whole cabinet made of powder coated cold rolled steel, with static finish stainless steel clad table top. Fitted with Prefilterand HEPA Filter of efficiency 99.99% with cold DOP and 99.97% with hot DOP to retain air borne particles of ≥0.3 μm Designed to meet US Federal Standard 209 B (BS5295). Blower & motor assembly statically anddynamically balanced. Auto switch off mode for blower motor when door closed, and interlocking of UV Germicidal Tube and Fluorescent Tube. Complete with Static Manometer, Cock for gas, air, or vacuum line, andside panels and front door made of 5 mm thick transparent plexiglass.Vertical GMP Certification is required. Working Size: W x D x H ; 900x600x600 mm 7. Specification GEL Documentation System Super large builtin 8" TFT LCD display, Exposure time adjustment, Saturation warning, USB media drive Network connectivity. 1D software with Image enhancement, annotation and illustration. 3 image analysis modules: - 1D Molecular weight (MW, volume, intensity, …) - Colony counting - Distance calculation (RF, IEF, …) Camera and optics Scientific Sony chip CCD camera with electronically variable shutter speed 1.4 megapixels, Massive 12-bit imaging for enhanced dynamic (4 096 grey levels) Dynamic range: 4.0 OD ,• Super-high sensitivity, • 6 positions filter wheel Transilluminator UV surface: 20x20cm with dual wave length, UV to blue conversion screen. 8. Ice Cuber Machine (Ice Cube Machine) Body mounted on heavy duty angle iron frame. Outer Body of Stainless Steel. Inner storage bin of Stainless Steel (SS-304).High density HCFC free PUF insulated storage bin.Unique modular design. Based on proven German technology. Automatic flushing of system after every cycle. Machine should not have coking nozzles, spraying noice and lime deposition. Water supply cut-off indicator. Systems controlled throught a sensitive temperature control unit. Ultra technology for lower power consumption. To work on 220 volts AC Supply. Capacity : 25Kgs/24hrs. 50Kgs/24 hrs. 9. Horizontal Electrophoresis Apparatus:1. Horizontal Multi Sub Midi with appropriate Power Pack for the system. : - Gel dimension 10x7 cm & 10x10 cm. UV tray, 2x 16 Sample, 1mm thick comb, casting dams, loading guide. 2. Horizontal Multi Sub Maxi with appropriate Power Pack for the system. : - Gel dimension 20x10 cm & 20x20 cm. UV tray, 2x 20 Sample, 1mm thick comb, casting dams, loading guide. 10. Vertical Electrophoresis Apparatus: Complete system MAXI Vertical Electrophoresis comprising with suitable Power Pack :- 1x omniPAGE Maxi Vertical unit with includes: 2x4mm thick notched glass plates,2x4mm thick plain glass plate with 1mm thick bonded spacers,1x dummy plate,2x comb,1x casting base, silicon mat, cooling pack, plus: 1x Capillary Electrophoresis Module which includes: internal running module for tube ges, capillary tubes, blanking plugs,2-D comb & spacers,1xElectroblottong Module comprising: internal electroblotting module, 3x compression cassettes for gel sizes up to 20x20 cm and 12x fibre pads. 11. Multi Sub Screen Electrophoresis Apparatus:- Multi Sub screen with appropriate Power Pack for the system : - Gel dimension 26x16 cm & 26x16 cm. 26x 32 cm UV tray, 6x28 Sample combs, 1mm thick comb, loading guide. 12. Slot and Dot Blotters: Slot and Dot Blotters with sutaible power pack : - 96-well Dot Blot mainfold 8x12, arry,6 mm diameter 12 mm deep, Vacuum required 600 mg Hg 0.8 BAR with cold trap. Unit Dimention:60 x105x140 cm, Member Size: 11x 7.4 cm. Slot and Dot Blotters with sutaible power pack: - All is above but 48-well Dot Blot mainfold 3x16, arry. Member Size: 12.1x4.4 cm. 13. Phase Contrast Microscope: - Standard binocular heads for photomicrography, 30° inclined eye tubes with adjustable diopter settings and interpupillary distance, 360° rotatable. EYEPIECES; Paired DIN 10 x with 20mm field of view. OBJECTIVE CHANGER; Smooth-operating, ball bearing mounted, reverse-angle, quintuple nosepiece. OBJECTIVE; BF Semi Plan Phase Contrast 10x, S20X, S40X, S100X oil. STAGE ;Flat-top stage: 171mm x 140mm surface with 80mm (X) by 52mm (Y) movement, graduated with vernier readings to 0.1 mm, detachable specimen guide, drop-down right of lefthanded controls. CONDENSERS ; Zernike type phase condenser, NA 1.25 with green interference filter 546nm, 29.8mm Ø.ILLUMINATOR ; Variable-intensity Koehler illumination for apertures up to 1.25. 12V 50W Halogen. 14. Ultra Centrifuge Refrigerated:Maximum Speed : 23000 rpm. Maximum RCF : 50000 G. Maximum Capacity : 500 ml x 6 tubes. Speed Selling Range : 100 rpm increment. RCF Setting Range 10 G. : 0:01 ∼ 9:59 (1min. increment), Time Setting Range : 10 ∼24hours (1hour increment), Temperature Setting Range : 8 to 30 °C (1°C increment) Safety Devices , , : Lid Inter lock System, Lid Open / Close Detector, Over-speed Detector, Imbalance Detector, Over-current Detector, Rotor Identification system Motor Over-current Detector, Temperature Error Detector Motor FIXED ANGLE ROTOR:1.Maximum Capacity : 6 X50ml RPM : 22,000 RCF : 50,000 2 Maximum Capacity : 24 X1.5ml RPM : 20,000 RCF : 43,000G RPM : RCF : 4,670G SWING OUT ROTOR Maximum Capacity : 50 X4ml 5,000 15.Refrigerated Multipurpose Table top Centrifuge without rotor, Technical Specifications: Maximum Rotational Speed: 14,000 rpm ( Speed canbe set from 200 -14,000 rpm in 10 rpmincrements) Maximum Centrifugal Force: 20,800 x g, Temperature Range: -9 °C to +40 °C Sample temperature: < 4 °C at maximum speed Simple programming with 35 programs memorywith write protection Automatic rotor recognition with speed limitationfor maximum safetyAutomatic Imbalance etection and cut – off. As per the requirements of IEC 1010-2-020 safetystandard. Motorized lid latch. Standby cooling maintains temperature whencentrifuge is not in use. ECO shut-off for reduced energy consumption . Built in condensate drain to eliminate wate Display shows Speed, RCF, Time and temperature. Acceleration and Braking Ramps for sensitive sample material. Optional RS-232 C Interface for connec Swing-bucket Rotor, including 4 x 100 ml rectangular buckets, 1 No. Maximum Rotational Speed (rpm): 5,000 min -1; Maximum Centrifugal Force: 4,400 x g Adapter for 7 - 17 ml tubes (Set of 2), For use with 100 ml rectangular bucket in rotor No. of tubes per rotor: 24 ; Maximum Rotational Speed (rpm): 5,000 min -1 Maximum Centrifugal Force: 4,200 x g Fixed-angle rotor (F-45-30-11) with lid for 30 x 1.5 / 2ml tubes, 1 No. Max. Rotational speed: 14,000 1/min ; Max. RCF: 20,800 x g Rotor, lid and adapters should be autoclavable. 16. Thermal Cycler Gradient Specifications:Universal Block for 96 x 0.2ml PCR Tube, 71 x 0.5 ml PCR Tube, One 8x12 PCR plate Gradient PCR, capable of testing 12 different temperatures simultaneously across a gradient range of 1 20° C Heating and cooling via peltier technology. Patented Triple Circuit Technology, ensures precise control of temperature Temperature control range: from 4 °C to 99°C Temperature Control Mode: Fast, Standard and Safe Lid Temperature range: 37 - 110 °C Block Temperature Accuracy: ± 0.2°C Block Homogeneity: 20° C to 72° C: ≤ ±0.3° C, 95° C: ≤ ± 0.4° C Temperature control speed: approx. 3 °C/s (heating) Temperature control speed: approx. 2 °C/s (cooling) Lid descent and closing pressure - Flex lid technology with Thermal sample Protection Intuitive Graphic programming with larger display Preprogrammed template for easy selection from 16 temperature protocols viz. 2 step PCR, 3 Step PCR, Gradient PCR, Long Range PCR, Low volume PCR, RT, RT-PCR, Incubation, Cycle sequencing, Touch down PCR, Hot Start PCR, Hot Start PCR manual, Large volume PCR, Nested cycles, reduced ramping, simulate Master cycler. System memory of more than 100 User folders and more than 700 programs Two USB ports: for Protocol transfer, Self-test, USB, printer / mouse E-mail Notification Interface: Ethernet, CAN in, CAN out, Power supply: 230 V, 50-60 Hz calibration according to NIST (USA), DKD/PTB (Germany) UKAS/NPL (UK), UL/CUL listed 17. SPCIFICATION FOR REFRIGERATED TABLE TOP CENTRIFUGE                   Table top centrifuge for high volume applications Temp. range –9 to + 40 deg C RCF should be > 20,000 g Max capacity of machine should be 3 liters or more , with at least 16 MTP plates. Rotors to maintain 4 deg C at max speed. Rotors should fully auto-clavable with aerosol tight lid facility. Rotor lid should come with Quick Lock facility for ease of use , and light weight rotors will be preferred. Should have short spin key, fast cool & stand by cooling option Facility to put “at set rpm” function, with ECO shut-off technology to reduce energy consumption and increase compressor life. Should have 10 acceleration & 10 breaking ramps for sensitive samples. More than 30 program memory Ability to spin 15 ml and 50 ml falcon tubes and oak-ridge tubes at 12100 rpm or more and 20130 x g or more with fixed angle rotor Facility for min no of rotors with max no of adaptors should be provided. Should be approved from IVD (In Vitro Diagnostic) Should have facility to validate speed temp and time with certified device. Should have the standard of International safety parameter. Must have built-in condensation drain facility to eliminate water accumulation inside the chamber. Warranty 1 year. Minimum Rotors required :  6 x 85 ml Fixed-angled Rotor with Max. RPM 12000 & RCF 18,500 xg with adaptors for 15ml & 50 ml falcon tubes(6 each).  Fixed angle rotor for 30x1.5/2.0ml tubes(rpm:14,000;20,000xg) Micro Refrigerated Centrifuge: Specifications:      Temp. range –0 to + 40 deg C RCF should be > 16,873x g. Speed 100-14,000 1/min. Max capacity of machine should 18x1.5/2.0 ml. Timer (with continuous mode) 30 s to 99 min. Acceleration to reach max speed in 13 sec  Breaking time max. speed 13 sec.      Number of Rotors 1 (max=7.7 cm) Max.rotor capacity 18x1.5/2.0 Ml tubes. Max Power requirement should 320 w. Unique Quicklock rotor lid for fast and reliable lid locking. Automatic door opening after the run. SPECIFICATION FOR ULTRA LOW TEMPERATURE DEEP FREEZER TYPE : Upright CAPACITY : 410liter OPERATING TEMPERTURE : UP TO –86°C with 1°C increment. POWER CONSUMPTION : 525 W/H ELECTRIC SUPPLY : 220V/50Hz, 13 Amps. single phase  Fully programmable microprocessor controlled with membrane keypad and eye level control panel.  Freezer should hold minimum of 240boxes & 24,000 vials per freezer.  System should have 304L grade stainless steel interior and tough, powder coated exterior finish constructed on steel gauge of 18g ZINTEC 1.2 mm thick.  Freezer should have five insulated inner doors with 4 adjustable shelves.  Freezers should have heated air vent to prevent vacuum formation and front panel air filter.  Heavy duty lockable castors and lockable outer doors and lids.  Freezer must have battery back-up and 4 PIN security lock for unauthorized tempering.  Freezer should have RS 232 interface data logging port & on board diagnostic software on control panel.  Audible and visible alarms for temperature, power failure, system failure, battery low etc.  Freezer must use CFC-FREE, HCFC-FREE non flammable refrigerants, and refrigeration system must be energy efficient and hermetically sealed cascade refrigeration system. Compressor should be capable to run any voltage between 190 – 270V. Freezer must have ISO 9000 safety requirements and IEC 61010 Electrical safety CE certified.  Freezer must have washable condenser filter indication which should keep fins free of dust to maintain peak cooling efficiency.  All vendors are requested to attach original technical literature/catalogue in support of the mentioned specifications & highlight the above features.  Freezer should be warranted for minimum 12 months & compressor for 5 years from the date of installation. Specification of Stackable Refrigerated Incubator Shaker Shaking Speed : 25 to 400rpm with ± 1 rpm control with shaking orbit 1”(2.54cm). 1) Temperature Range : 20°C below ambient to 80°C. Temperature accuracy should be within ± 0.1°C with a uniformity of ± 0.25°C. 2) Drive Mechanism : Triple-eccentric counterbalanced drive with 9 permanently lubricated ball bearings. Solid state brushless D.C. motor. 3) Should have an adjustable-height shelf for added incubating or storing capability. 4) Alarm should indicate when speed deviates more than 5 rpm or temperature deviates more than 1°C from set point, and when timer operation has expired. 5) It should have easy to use programmable controller automatically changes temperature, speed at timed intervals, hold upto 4 programs, 15 steps each, can eliminate un-necessary return trips to labs, automatic heat induction studies and many more. 6) Shaker should have a Multi-function reservoir for chamber humidification; reduced sample evaporation and unit protection from spills. 7) Platform : Should be 46cm X 46cm or higher. Universal platform should be able to hold at least 20 ± 2 clamps of 250ml. 8) It should be possible to use the shaker on or under the bench or double stacked for space savings. System should not be more than 35” high. Shaker should have an option for having 12-Port Gas Manifold, Humidity Monitor, UV Germicidal Lights and Culture Drawer. 9) It should be CSA, UL & CE certified. 10) It should have output signal to RS 232 enables data logging and computer controlled. 11) Clamps and test tube racks of various sizes having single piece construction and with spring retainer should be quoted as options to choose. 12) System should be warranted for 12 months from the date of installation. Specifications:- Universal Block for 96 x 0.2ml PCR Tube, 71 x 0.5 ml PCR Tube, One 8x12 PCR plate Gradient PCR, capable of testing 12 different temperatures simultaneously across a gradient range of 1 20° C SteadySlope Gradient Technology ensures ramp rates are identical in both gradient and normal operation Heating and cooling via peltier technology. Patented Triple Circuit Technology, ensures precise control of temperature Temperature control range: from 4 °C to 99°C Temperature Control Mode: Fast, Standard and Safe Lid Temperature range: 37 - 110 °C Block Temperature Accuracy: ± 0.2°C Block Homogeneity: 20° C to 72° C: ≤ ±0.3° C, 95° C: ≤ ± 0.4° C Temperature control speed: approx. 3 °C/s (heating) Temperature control speed: approx. 2 °C/s (cooling) Lid descent and closing pressure - Flex lid technology with Thermal sample Protection Intuitive Graphic programming with larger display Preprogrammed template for easy selection from 16 temperature protocols viz. 2 step PCR, 3 Step PCR, Gradient PCR, Long Range PCR, Low volume PCR, RT, RT-PCR, Incubation, Cycle sequencing, Touch down PCR, Hot Start PCR, Hot Start PCR manual, Large volume PCR, Nested cycles, reduced ramping, simulate Master cycler. System memory of more than 100 User folders and more than 700 programs Two USB ports: for Protocol transfer, Self-test, USB, printer / mouse E-mail Notification Interface: Ethernet, CAN in, CAN out Dimensions: W: 25 cm, D: 41.2 cm, H: 32.1 cm Weight: 10.9 kg Power supply: 230 V, 50-60 Hz Power consumption: 700 W Calibration according to NIST (USA), DKD/PTB (Germany) UKAS/NPL (UK), UL/CUL listed Minispin Plus  Compact centrifuge for speed up to 14,500 rpm  Rotor for 12 X 1.5 ml/ 2 ml tubes  G value should be ≥14,100  Timer from 15 sec to 99 mins, with continuous function  Adaptors for 0.2, 0.4, 0.5 ml & 0.6 ml Microtainer tubes(optionally)  Acceleration to reach max speed in 13 sec  Breaking time max. speed 12 sec.  Automatic door opening after the run  Brushless motor  Autoclavable rotor (121o C, 20 mins)  Stain Less Steel Rotor lid with knurled nut  Weight less than 4.5 kg.  Bore size should be 11 mm.  Fixed angle rotor should be at 45o  Should confirm to IEC 1010-2-020 safety regulations.  Auto Opening of lid after the run.  Separate short spin button.  Metal rotor housing. Smooth Power Supply System Features: On-lineSine Wave UPS with Surge Protection and Filter with a suitable Sealed Maintenance Free Leak-proof Lead-acid Battery. Control Panel with display for ‘On Mains’, ‘On Battery’, ‘Replace Battery’, ‘Over Load’, and distinct audio-alarm for ‘On Battery’, ‘Low Battery’, and ‘Over Load’. Protection Class: IP 20. Capacity: 10 kVA Specifications: (A) Input: 160 - 280 V, 50 ± 5 Hz (auto sensing), Full Load Total Harmonic Distortion: < 7% (B) Output: 220/230/240 V, 50 ± 3 Hz, (C) Back-up: 45 minutes @ full load, and 5 hours @ 1/4 of full load. Voltage Distortion: < 3% (D) Recharging Time: 150 minutes approx. (E) Noise Level: 55 dBA @ 1 metre distance. Bench Top Ultrapure Water System Features: Suitable for inlet water of tap-water quality to deliver ASTM Type 1 water for use in Research Laboratory. The equipment should comprise of 1. Suitable Pre Filter for connection with tap water supply. 2. First stage of water purification to deliver ASTM Type 2 water consisting of Particle Removal Filter, Softener, Iron Remover, and Booster Pump. 3. Intermediate Storage Tank for water of ASTM Type 2 quality. 4. Final stage of water purification to produce water of ASTM Type 1 quality. 5. Microprocessor based touch button controlled digital display of set and actual parameters of water quality. Specifications: (A) Feed Water Tap Water available in institute. (B) Final Output Flow Rate: 0.1 to 2 litre / minute adjustable, Conductivity: < 0.055 µS/cm, Resistivity: > 18.2 M Ohm.cm, Particle Content: < 1/ml, Micro-organism Content: < 1 CFU/litre, RNase Content: < 4 ng/litre, TOC: < 2 ppb, Endotoxins: < 1 EU/litre, DNase Content: < 24pg/ml