Transcript
OLYMPUS INVERTED RESEACH MICROSCOPE
~~ODEL
INSTRUCTION MANUAL
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OLYMPUS
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Thi s inst ruct ion manual has been prepared f o r the O ly mpu s Invert ed Research Microscope Model IM T . It is recomm ended th at you read the man ual caref ull y in o rder to famil iar ize you rself f ull y wi t h you r m icroscope in order to ob t ain opt im um perfo rmance f ro m t hi s precision inst rument .
IMPORTA NT Observe th e following points car efully: •
Operation 1. A lways handle t he m icros cope w it h the care it deserves, and avo id abrupt m otions . 2 . A vo id exposure of the m icros cope t o direct sunlig ht, du st and vi brati on. 3 . Only use the te nsion ad just ment ring for altering the t ension of th e coarse adj ust me nt kn o bs. Do no t twist the two coarse adjustment kn obs in th e op pos ite d irecti on s sim u lt aneously, as th is causes damage. 4 . Ascerta in that t he voltage select or swi tc h on th e base plate is set t o co n fo rm w i th t he local mains voltage. 5 . Di sconn ect the li ne co rd fr om the AC power ou t let for fu se repl acem ent . 6 . Object ives provided to t he Mod el IMT are designed for observat io n o f speci mens t hro ugh cu lt ure vesse ls of 1 mm th ickness. Therefore , be sure to use cult ure vessels, th e b ottom o f wh ich is 1 mm ±O.3 t hic k and even ly f lat .
•
Main te nance 1. Len ses mu st always be kept c lean. Fine du st o n lesn surfaces sho uld be b lown or w iped off by means of a hand bl ower or a clean bru sh. Carefully wipe o ff oil o r fingerp rin t s deposited o n t he lens surf aces wi t h gauze moi stened w ith a small amount of x y lene, alcoh o l-et her m ixture (7 : 3) or et her. 2 . Do no t use o rganic solutions t o wi pe t he surfaces of vari ous com pon ents. Plast ic part s, especi all y, sho uld be cleaned wi t h a neut ral dete rgent. 3 . Never di sassemb le th e mi crosco pe f or repair . 4 . The m icroscope shoul d be st o red in it s con ta iner im med iat ely aft er use. If this is not possible, it shou ld be co vered with the vi ny l dust cover provided . It is best to keep objectives and ey epieces in desiccat o rs. co n taining desiccants such as sili ca gel.
CONTENTS
I.
ST A NDA RD EQUIPMENT
2
II .
NOMEN CLATURE
3
III.
A SSEMBLY
4
IV .
IDENTIFI CATION AN D FUN CTI ON OF VARIOU S COMPON ENTS .
6
V.
OPERATION . A.
B.
C.
D.
E.
F.
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10
Electri c Equipment
1.
Adjustment of Mi nimum Vol tage
2.
30W Tungsten Bulb
Specimen Holders
1.
With the Mechanical Stage
2.
Without the Mechan ical Stage
Obse rvatio n Tubes
.
.
.
.
.
.
1.
Interpupillary Distance and Diopter Adjustments
2.
Eyepiece Cap
11
Illumin ation for Phase Contrast Microscopy
1.
Ultra Long Working Distance I llumination
2.
Long Work ing Distance Illuminat ion
12 13
Field and Aperture Iris Diaphragms
1.
F ield Iri s D iaphragm
2.
Aperture Ir is Diaphragm
Fo cusing Adjustm ent
.
.
.
.
.
.
.
.
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.
.
1.
Tensi on Adju stment of Coarse Adjustment Knobs
2.
Au tomatic Pre-focusing Lever
3.
Ad just ment of Stage Block Height
14
V I.
OPTICAL DATA
15
VII.
T ROU BL ESHOOT I NG
16
I.
STANDARD EOUIPMENT
Model Component
IMT 201
203
2 11 213
M icroscope stand with p illa r and base
IMT ·F
1
1
1
1
Revolving nosepiece
IMT·R E
1
1
1
1
Binocular tube
BH·BI 45
1
1
1
1
Plain stage w ith two extension plates
IMT·S
1
1
1
1
Me chanical stage with three specime n ho lders
IMT·MV R
1
1
Condenser holder
IMT ·CH
1
1
Lo ng wo rking distance tu rret condenser
IMT·LWCD
1
1
1
1
1
For ult ra long work ing d istance
IMT ·U LWD
For long worki ng di stance
IMT·LWD
Light annul us
IM T·RS-lO
Light annulu s
IMT·R S-20
1
1
1
1
IMT·RS-40
1
1
1
1
Lamp hou se
IMT·LH
1
1
1
1
30W tungsten bulbs
LS30
3
3
3
3
Ach.4X
1
1
1
1
Ach. l OX
1
1
1
1
Ach. LWD·C20 X
1
1
1
1
,
Auxi liary lenses
Light annul i in
mount IMT·RS
Light annu lus
Object ives
1
1
1 1
1
1
1
Ach. LWD·C Plan 40 X Eyepieces BiCK10 X, paired
1
1
1
1
1
Phase annulus slide
IMT·SL
1
1
1
1
Centering telescope
CT
1
1
1
1
Spare fuses 100 -1 15 A ( lAI or 220 -240V (0.8AI
2
2
2
2
Ey ep iece caps
2
2
2
2
1
1
1
1
1
1
1
1
45 G-533
G reen filter V inyl dust cover
• Optional Accessories: 1. Trinocular Tube
Mod el 8 H·T R45
2. Phot o Eyepieces
FK2.5 X , FK 3.3 X , FK5X , FK 6.7X
3. Photom icrograph ic System Camera
Model PM· 'O
4. Drawing Attachment Mod el 8 H·DA (Thi s att achment is provided with a magnif ication adjustment screw for use not only with th e Mod el IM T but also w it h vari ou s Olympus mi croscopes such as VANO X , BH, CH, etc . For use with the Model IMT the adjustment sc rew on the drawing attachment shou ld be set to the posit ion "C".)
2
I I.
NOMENCLATURE The Model IMT is composed of var ious co m po nen ts and interch angeab le accessor ies . A var iet y o f com binations. stan dard o r, optio nal , is avail able acco rdi ng to y ou r requ irement s .
Lamp ho use 30W tungsten lam p
Plain stage Pillar
t
Mechanical stage A ttach able .
Auxi liary len s For lo ng w o rki ng
distan ce.
Ey epiece
Long wo rki ng d ist an ce
tu rre t conde nse r
Con den ser holder
Ob je cti ve Obser vation tu be Revolving nosepi ece
Phase annulus slide
Sta nd
• Base
Ligh t an nuli for ultra
Auxi liary lens
lo ng wo rki ng d istance
For ultra long wor k ing
d ist ance.
3
III.
ASSEMBLY
*
Th e picture below ill ust rat es th e sequent ial proced ure of assem bly . Th e number s ind ic ate th e assembly order of various co mponents. Remove du st caps before mou nting co m ponen ts. Take care to keep all glass surfaces clean and avo id scrat chin g the lens surfaces.
@] @ Mech an ical stage (See NOT E 2.1
@ Specimen holder
f!
1
~
cl am p i ng
0
screws C lam p steadi ly w i t h a coin.
@ Eyep iece
E 5 ==
J ® Co nde nser
@ Light a n n u l u ~
® Lam p ho u se
holde r
~~~:;~:r
@ Eyepiece ca p
t u ngs te n bul b
!
Q) Co nd enser
Stage
® 3M
TI~
T ap ped ho le
Jl~:~; -J lv wit h a
coi n.
N osepiece
Clamp w it h sc rew prov id ed .
Pillar clamping sc rew Lo wer limit st o p pi n K nu rled ring
Rotate t he ring t o sta bilize t he base.
NOTE 1:
The lower end of t he p ill ar has tw o notches (larger one at t he fr on t and smaller one at the back ) and a co nnect ing co rd co mes out of it . Insert th e co nnect ing co rd and low er end of the pi ll ar into th e base hol e vertically and clamp th e p illar with screw at the bottom of the base. The p illar d irect ion is dete rm ined by f itt ing the no tches so th at the co nnect ing cord passes through the larger no tch and co nnects to the
receptac le o n the left hand sid e of the base back .
4
NOTE 2:
Th e mec hanical stage is attachab le t o the rig h t o r left hand side o f the plai n st age an d clamped w ith a co in. If t he mecha nical sta ge is attached, o nly one exten sio n p l at e can
be attac hed to t he o pposite side of t he pla in stage. @
How to mount t he mechanical sta ge: F ig. 1 shows t he mechanic al stage attached on t he ri gh t hand side o f t he p lain s tag e. It is po ssibl e to attach th e mechanic al stage o n t he left side as shown in Fig. 2 ac co rd in g t o your prefere nce . To co rrect ly attach t he mechan ical staqeon eit her side, al ign t he edge o f t he mechan ical st age wi t h the plain stage co rner as indicated by arrow s.
F ig. 2
Fi g. 1
To t ho se w ho w ish to use a left-han ded mech an ical stage, the mechanical sta ge w ith left-ha nd low dr ive contro ls Mod el IMT· MV L is op ti onall y avail abl e.
NOTE 3:
Set up the illuminat or: a) T o illuminat e f or lon g work ing distance ( Fig. 31: Mou nt t he aux il iary lens IM T -L WD CD t o the lam p hou se suppo r t ® and attach th e consenser and co ndenser ho lder t o t he plain stage; th en adj ust the heigh t of t he suppo rt ® , movi ng it ver t ically alon g t he pi llar un t i l th e lo we r edge o f t he suppo rt is aligned w ith t he ye ll ow l ine ® o n t he pill ar and d amp.
Fig . 3
Fig , 4
b) T o illumin at e f or ul tra long w or k ing distan ce ( Fig. 4 ): Att ach t he auxi l iary lens IMT -ULM D CD to t he support @ and align th e low er edge of the support t o t he red line ® . For t h is use, t he con denser and con denser hol de r should be removed . cl For use of t he Abbe cond enser BH ·eD or A ch rom atic/ aplan ati ccond enser BH ·AAC : It is necessary t o moun t the op ti onal au x ili ary lens IMT ·A L t o t he lamp h ouse supp ort and attach th e con denser and con denser ho lder t o the stage. Th e lower edge of t he suppor t should be alig ned wi t h t he red line on t he pi l lar. For observat ion wi t h object ives 40 x o r h igher , use an ordinary object ive and pu t a cover glass under t he specimen .
*
d ) For use of th e di fferentj al 1~terference con trast attachment BH·NIC : It is necessary to ali gn t he lower edge o f t he support wit h the bl ack index li ne on the pillar, and use t he au x ili ary lens IMT - AL . On ly a cover glass of 0 .17 m m t hick can be used .
*
5
IV.
IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
Filter mounts Inser t the green fil t er 45G -533 in t o one o f t he tw o m ounts f o r
L am p house support
phase co ntrast observat ion.
Fi eld ir is diaphragm lever Sup p o rt locki ng knob
Interpup illary distance scale Aperture iris d iaphragm lever
Mechani cal t ube length adjust ment rin gs
Conden ser height adjustment kn ob
Low drive coax ial cont ro l kn ob s
Ten sion adjustme nt ring Arrow mar k ind icat es increase in coarse adjustme nt t ension .
Coarse adj ustme nt kn ob
/.-N__- -- -
Fi ne adjust ment knob Graduated in in cre ment s of
211 .
Gro und term inal
Pil ot lamp
Main swi tc h Sliding cont ro l lever A s numbers in crease. vo lt age in-
creases.
6
Stage cl ips
I
Index li nes
Stage ex tensio n p late
Light a nnulus
centering screw Phase ann u lus
insert io n slo t Condenser center ing
screw
Connect ing cord
Nosepiece c lam p in g
screw
Level screw Rota te t he ri ng t o
level t he base .
L ine co rd recept ac le
7
III Aux iliary lens cla m p ing scr ew
----1_-·' --------~J] _.-:L~ lgh~ta:::.:.:.nn:::::ulu::::....:sc:..=.. l am2.P I~ ng_ sc r_ ew
111'
Ligh t an nulus cent er ing screws
Rheost at t rimmer screw A fter sw itching on, if necessar y ,
rotate this screw with a co in unt il t he b u lb
is d imly li t w i th the
slidi ng co n tro l lever at minimum vo lta ge p osition.
Line voltage selecto r swit ch Se t t h e sw itc h to con form w ith
Fuse ho lde r
line vol t age.
Press wi t h a screw d ri ver and ro-
tat e co unte rc lockwise t o rem ove the ho lder.
8
SUMMAR Y OF PUTTIN G THE MICROSCOPE IN OPERATION Model IMT
A.
Mat ch t h e li ne voltag e selector switc h t o local main s vo ltage (page 8).
B.
Switch on th e light so urce.
C.
Rotate t he trimm er screw until th e illum inator dim ly li gh t s with slidi ng co nt r ol le ver at m inimum voltage po sition (page 10 ).
D.
A lign t he lamp ho use suppor t to t he index li ne on th e pill ar acco rd ing t o your m icr oscopic
purpose (page 5). E. F.
Place a spec ime n on th e stage (page 10 ).
*
Coarse fo cu s with th e lO X o bject ive. If necessary, sto p down th e aperture diaphra gm to r ea sier fo cu sing . Fo r ph ase co ntrast , ho wever, mak e it a po int o f opening th e full ape rtu re.
G.
Mak e interpupi llary and di opter adju stments (pag e 11 ).
H.
Ad just condenser heigh t if t he lo ng wo rking di sta nce co nde nse r IMT*LWCD is used (page 13) .
I.
Engage the pha se annu lus " 10 " .
J.
*
Engage t he light ann ulus " 10". For th e use of th e au xiliary lens IMT· U LWD, engage th e light annu lus RS·l 0 .
K.
Center t he light ann ulus by means of th e centering telescope CT (page 12 ).
L.
Sw ing the objecti ve of desired magnifica t io n into light path .
M.
Match the light ann ulus to t he magn ification o f objective in use and cente r t he an n ulus.
N.
Adjust light int ensity.
O.
Fine focus.
P.
Adjust aperture d iaphragm and fie ld diaphragm (page 13 ).
(Cu t o ff t h is page at d o tted li ne and pu t it o n th e wa ll n ear th e m ic rosc ope to see as a rem i nd er o f m icr osco pic procedu re .l
9
V.
OPERATION A. Electric Equi pment 1. A d ju stmen t of Minimum V ol t age
The m inimum voltage req u ir ed fo r t h e lig h t sour ce can be adj u st ed w i t h t he rh eo st at tr immer screw at th e mi cro scope base p lat e in accordance w ith t he li ne voltage and f req uency . Th e bu il t- in rheostat in co rp o rates a thy r ist or in i ts sem icond ucto r c ircu it fo r
the follow ing adv antages : a) Ex t rem ely fi ne adj ustment of l igh t intensity . b ) Elimination of f l ick er and stab i lizat io n of ligh t in ten si ty .
c ) Increased l if e ex pect ancy of th e bul b. For adju stm ent of t he minimum li ne vo lt age, ascertain that the vo ltage selector swi tch is set to con form w ith t he lo cal main s v o lt aqe, and t he slidi ng co ntro l lever ® is positi oned clo sest to yo u (l ow vo !taqcl , and then activa te the m ain sw itch CD . If th e bu lb is
F ig. 5
di mly lit, t he seconda ry vol t age is co r rect. I f it is not l it at all, rot at e t he rheost at t rimm er screw ® grad ually wit h a co in, un ti l t he bulb is d imly l it ; t hen push t he slid ing co nt ro l lever fo rwa rd in orde r t o obtain o pt imum li gh t
inte ns it y . {Fig. 51 2. 30W Tun gst en Bulb T he stand ard li ght sou rce incorporates a 30W pre -cen tered tu ngst en fil ame nt bul b, pr ov ided w ith a socket for pos it ive co nt act , el im inati ng t he prob lems of defect ive co nta c t and over -heat ing. When used at t he sett ing " 6 " on the contro l lever sca le, th e average life o f the bu lb LS30 is lon ger t han 200 ho ur s. T hi s is, h owever, greatly reduced, if the bulb is used at higher vo ltage. Theref or e, it is adv isable t o avo id prolon ged use at read ings over "6" (in the red zon e).
*
It is recommend ed t o avo id sw it ch ing on and off th e tungsten bu lb wit h th e slidi ng co ntrol lever at high int ensit y pos ition (away fr om th e u ser ). It red uces bu lb life.
B. Specim en Ho lders
1. With the Mech anical Sta ge Mak e a cho ice of spec imen h o lder s accord ing t o your speci men and p lace i t on t he stage.
Stage p late ho lder
60m m d ia .
SIide h older
Petr i d ish holder
2. Wit ho ut th e Mechanic al Stage Place a specimen on the p lain stage di rectl y and move it manuall y . @ Thi ck ness of cu lt ure vesse ls
Ob jectives provided w it h t he Mo del IMT ar e designed t o observe specim ens t hrough 1 mm t h ick glass. Make it practice , the ref ore, t o use cultu re vessels or slides o f thi ckn ess 1 mm ± O.3.
10
@ Use of th e corr ect io n colla r of th e object ive LWD ·C Plan 40 X
After co arse and fine ad just ments , rota te t he co rrect io n col lar . keep ing the specimen in fine focu s until op t imum resoluti on is o b tained . A p ro per use o f th is coll ar is specia lly ef fecti ve t o prevent th e deteri ora tio n o f t he ob jective resol ution to be caused by t he un even th icknesses of various pet ri d ishes, cu lt ure bottl es, etc . C. Observ at ion T ubes 1. Interpu pillary Dist ance and Diop t er A d justments 1) Hol d the knu rl ed sli d ing grips _ 1(08~ (
0.43 ---
(The resolving power is obtained at th e fu lly ope ned aperture d iap h raq rn.]
Techn ical terms: · Work ing distance .
Th e di stance from the specimen slide or culture vessel to the nearest poin t of the objective.
·
T he N .A . represents a performance number wh ich could be compared to the relative aperture of a camera lens. Th e quantity of light which the objective receives f rom the object increases w ith the square of the performan ce
Numer ical aperture
number . ·
Resolving power .
Th e resolving power of a lens is measured by its ab ility to separate t wo poin ts.
·
Focal depth
T he distance betwe en the upper and lower lim its of sharpness in the image formed by an opt ical system. As you stop down the aperture iris d iaphragm . the focal
dep t h becomes larger . Th e larger t he N.A . of an object ive th e shallower the focal dept h. ·
Field number .
A number that represents the diameter in mm of the image of t he f ield diaphr agm that is formed by the lens in fro nt of it.
·
Field of view di ameter .
The actual size of the fi eld of view in mm.
15
VII. TROUBL ESHOOTING Causes
T roub les
Remedi es
1. Opt ical Sy ste m a) Field of view is cut off or illum inat ed irregularly _
Auxiliary mounted.
lens is no t
co rrectly
Lamp house suppo rt is not pos itioned at cor rect height .
Nosepiec e does not click in p osition .
Correct ly mount the lens. (Page 5)
Ad j ust t he height co rrec t ly . (Page 5) Sl igh tly rotate the no sepiece unt il it clicks into posit ion.
ed to the ob servation purpose.
Co rrect it referring to " I I I. ASSEMB LY " at page 5.
Co nde nser is no t corr ect ly moun ted
Remount th e condenser corre ct ly .
Illuminat ion is not correct ly match-
on the holder.
F ield d iaph ragm is stopped down
excessively . Bulb is not correctly att ached.
Open th e d iaph ragm properl y . (Page 13 ) Re-insert it into t he socket all the
way . Phase annulu s slide is not click stopped in posit ion .
b) Dust or dirt
is visible in
Cli ck it into position .
Dust on condenser top lens.
the field of view. D irty speci me n.
Rem ove d ust .
D ust on eyep iece.
c) Excessive image contrast.
Co nde nser
is excessively
in h igh
Low er the co nde nser.
position. Apert ure ir is diaphragm is stop ped
Open th e d iaph ragm . (Page 13 )
down ex cessively.
d ) Resolutio n problems:
• • •
O bject ive is not co rrec t ly posit ion -
Slightly ro tate the nosepi ece unt il
ed in l ight path .
it cl ick s into posit ion .
In case of LWD·C Plan 40 X , cor-
A djust th e co llar co rrectl y . (Page 11)
Image is not sharp . Insuffici ent cont rast.
I mage detai ls lack
ce-
rect lo n co lla r is not adj ust ed .
f init io n. D ust on object ive fro nt lens.
Clean.
Dirty spec imen . Dust on condenser lens. Illu m inat io n is not mat ched to o bservat io n pu rpose.
th e illum inat io n ref erring to " I II. A SSEM B LY" at page 5.
Cor rect
Speci me n slide is mo unted on the
Reverse th e specime n slide. (Page
stage upside do w n.
10)
16
T roubles
Causes
Remedies
e) Specimen image is part ialIy o u t of f ocu s.
Objective is not correctly positioned in li ght path .
Slightly rotate the nosepiece unti l it clicks into positio n.
Specimen is not correctly posit ioned on the stage.
Piece the specimen correct ly on the st age. (Page 10)
f ) When objectives are changed they are not par fo cal.
Mechanical tube leng th is not correctlyadjusted.
Adju st the length w ith th e tube length adjustment rings on observat ion tube. (Page 11)
gl No effective phase contrast is obtained.
A perture iris diaphragm is stopped down .
Open .
Object ive, l ight annulu s and pha se annulus are not correctly matched.
Mat ch them cor rect ly . (Page 12)
Light annulus and phase annulus are not correctly centered .
Cen ter th em co rrec tly. (Page 12)
V olt age selector switch matched to mains vol tage.
is no t
Ma tch the selector sw itch to mains voltage.
Mains voltage is too high (or t oo low ).
Adjust mains voltage with a variable vo ! tage transformer.
Rheostat trimme r screw matched to mains voltage.
is no t
Adju st t rimmer screw unt il the bu lb is dimly lit w ith slid ing control lever positi oned at minimum vo ltage. (Page 10)
Vo ltage selector sw itch matched to mains voltage.
is no t
Match the selector switch to mains voltage.
Mains vol tage is too low (or too h igh).
Adj ust mains voltage w it h the varl able voltage transformer.
Ma ins voltage is unstable.
Use a voltage stabilizer.
T he f il amen t o f bulb is likely t o burn out.
Rep lace the bu lb .
Loose electric connect ion.
T ighten the connect ion.
Fuse is not a standard one.
Use a standa rd fuse.
2. Electric System a) Illuminator is to o br ight (or too dark ).
b) Vo ltage f or illuminator cannot be regulated.
cl T he ligh t f lickers and th e intensity is unstable.
d l Fuse burns ou t too often.
V oltage selector switch matched to mains voltage. e) Pil o t lamp l igh ts bu t iIluminator bulb does not. f ) Reduced bu lb l if e.
is
not
Match the switch to mains voltage.
Th e bulb is burned out.
Repl ace th e bu lb .
Loose electr ic connectio n.
T ighten the connection.
Vo ltage selecto r switch matched to mains voltage.
is
Th e bulb is not a standard one.
17
not
Match the selector sw itch to mains voltage. Use a st andard bu lb.
Causes
Remedies
Bul b is used on higher voltage to increase light intensity .
Keep the o utput vo ltage unde r 6V (wi th th e slidi ng cont rol lever at " 6" setting on the scale ) as much as possible, or use a high inten sity light source such as a halogen lamp, etc .
Ten sion adjustment ring is t ightened too much .
Loosen the tension adjustment ring properly . (Page 14)
Th e user is try ing to lower the stage passing over th e lower focusing limit imposed by the engaged prefocusing lever.
Unlock the pre-focusing lever. (Page 14)
b) Stage drops and specimen goes out of focus.
Th e tension adjustment ring is too loose.
Ti ghten the ring pr op erly . (Paqe 14)
c) Stage cannot be lowered to the lower limi t of th e work ing range.
Pre-focusing lever is engaged in higher than focusing position.
Unlock th e lever . (Page 14)
d) Stage cannot be raised to the upper lim it .
Stage bl ock is not aligned to the correct stage block height index .
Adjust the stage bl ock height correctlv . (Page 14)
Interpup illary d istance is not correctly ad just ed.
Correct the interpupillary distance. (Page 11)
D iopt er adjustment is incomplete.
Co mp lete d iopter adju st ment. (Page 11)
Th e user is unaccustomed to bin oGular vision.
Prior to loo king at specimen details, t ry to look at the ent ire field of view, or look at a far away object before resuming microscopic obser vation.
a) Image easily goes out of focus when yo u touch the stage.
Th e stage is not correctly clamped.
Clamp th e stage securely.
b] The specimen stops midw ay on the east-west traverse.
Specimen is not tioned on stage.
c) Specimen stops midway on the north-south traverse.
Mechanical stage is not mount ed in correct positi on.
Troubles
3. Focusing a) Coarse adjustment is too t ight.
4. Observation T ube a) Incomplete binocular visio n.
5. St age
correct ly
1B
posi-
Adju st specimen positi on.
M ount the mechanical stage in correct position. (Page 5)
MEMO
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OLYMPUS OPTICAL CO. LTD.
01VMPLJS ..:::tOKYo:..
43-2, HATAGAYA 2-CHOME,SHIBUYA-KU TOKYO, JAPAN.
Prited in Japan 7904 M 03
