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OPTOSPLIT II LS
D ATA S H E E T
Two-way image splitter NEW LARGE SENSOR VERSION
The Optosplit II LS image splitter from Cairn Research is a simple and elegant device for dividing an image into two separate, spatially equivalent, components that can be displayed side by side on a single camera chip.
Splitting is usually performed on the basis of wavelength, allowing applications such as ratiometric calcium imaging or FRET, however, polarising beamsplitters are also supported. The two images can be captured simultaneously offering a major benefit over manual or electronic filter changers. A rectangular aperture is used to define the region to be imaged, with a set of simple controls allowing the user to vary the relative positions of the two output images on the camera. The Cairn Optosplit II can significantly widen the scope of any fluorescence imaging system.
APPLICATIONS KEY BENEFITS
Ratiometric ion or voltage imaging Förster Resonance Energy Transfer (FRET)
Works with sensor sizes up to 22mm diagonal (eg 5.5MPixel sCMOS)
Dual probe widefield microscopy
TIRF/Spinning disk confocal
User configurable filter cubes with industry standard filters/dichroics
Combined fluorescence/transmitted light microscopy
Optional magnification or demagnification Unsplit mode through either channel or neither (18mm diagonal) Intuitive and independent x, y and focal controls Accommodates ND filters or chromatic correction lenses Standard spectral range from 450 to 900nm Supports cropped sensor modes
tel: + 1.802.881.0302 www.cairn-research.co.uk
email:
[email protected]
toll free 1.877.417.8313
www.89north.com 11/14
OPTOSPLIT II LS
D ATA S H E E T
Two-way image splitter NEW LARGE SENSOR VERSION
SUMMARY
In fluorescence imaging applications it is often useful to acquire simultaneous images at two emission wavelengths. Traditionally such applications have been restricted by the speed of an electronic filter changer, or by the cost and complexity of adding a second camera to a system. In many instances the region of interest (ROI) does not require the full resolution of the camera so the ideal solution would be to simultaneously image at two different wavelengths on the same camera chip. In conjunction with a research microscope and a suitable CCD camera the Cairn Optosplit II allows the researcher to do exactly this. The Cairn Optosplit II is usually supplied with a rectangular aperture to define a ROI and includes controls to allow the two images to be positioned accurately and conveniently within the camera frame. Images can be acquired using any imaging software and processed either manually off-line or using an appropriate analysis tool such as the Splitview module in Molecular Devices’ Meta series software or Field Split in Andor Bioimaging’s iQ. The instrument is usually configured to attach to the cmount output port of a research microscope, with a cmount CCD camera fitted to its output. The design allows for connection to a variety of alternative devices, so please consult with us if you intend using it in any other configuration.
320mm 110mm
60mm
110mm
Maximum depth 79mm
Base units P280/210/0LS
Optosplit II 'LS' emission image splitter ( x1.0 magnification) Includes rectangular input diaphragm, calibration cube & one shutter plate. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
P280/210/MLS
Optosplit II 'LS' emission image splitter ( x1.0 magnification) Includes OptoMask input diaphragm, calibration cube & one shutter plate. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
Cubes and options P290/000/200
Cairn emission / excitation filter cube for 25mm filters (Empty)
P290/CR2/012
Optosplit II corrector lens kit (Includes one full width holder)
P290/ND2/006
Optosplit II neutral density kit (Includes one half width component holder and 4 ND filters)
P290/POL/001
Cairn emission / excitation filter cube with integrated polarising beamsplitter cube
P290/POL/002
Rotating Optosplit auxillary component holder with 25mm polariser (Full width)
tel: + 1.802.881.0302 www.cairn-research.co.uk
email:
[email protected]
toll free 1.877.417.8313
www.89north.com 11/14
OPTOSPLIT III LS
D ATA S H E E T
Three-way image splitter NEW LARGE SENSOR VERSION
The Optosplit III triple image splitter from Cairn Research is a simple device for dividing an image into two, or three separate, spatially equivalent components which can be displayed side by side on a single camera chip.
Splitting is usually performed on the basis of wavelength and/or polarisation, allowing applications where there is a requirement for simultaneous, or high speed acquisition of multiple image emission bands or polarisation states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated in high speed stream modes and reduces demands on the software.
APPLICATIONS Triple fluorescence probe studies Polarisation Förster Resonance Energy Transfer (pFRET) Ratiometric ion imaging Polarisation studies Simultaneous phase contrast and fluorescence Multi-depth imaging
KEY BENEFITS Works with sensor sizes up to 22mm diagonal (eg 5.5 MPixel sCMOS) Dichroic mirror and emission filters mounted in readily interchangeable cubes for dual or triple emission separation Unity magnification fitted as standard, other options on request Simple intuitive alignment in x, y, and focal planes Interchangeable mount for N.D. filter or chromatic correction lens Bypass mode to allow single wavelength imaging using either pathway (18mm diagonal) C-mount coupling to microscope or camera lens Supports cropped sensor modes
tel: + 1.802.881.0302 www.cairn-research.co.uk
email:
[email protected]
toll free 1.877.417.8313
www.89north.com 11/14
D ATA S H E E T
OPTOSPLIT III LS
Multidepth imaging
SUMMARY
In fluorescence imaging applications it is often useful to acquire simultaneous images at multiple emission wavelengths. Traditionally such applications have been restricted by the speed of an electronic filter changer, or by the cost and complexity of adding extra detection hardware. In many instances the region of interest does not require the full resolution of the camera so the ideal solution would be to simultaneously image at multiple wavelengths on the same camera chip. In conjunction with a research microscope and a suitable c-mount camera the Cairn OS III allows the researcher to do exactly this. The image splitter is usually supplied with unity magnification and fitted with a rectangular aperture to define the ROI. It includes controls to allow up to three images to be positioned accurately and conveniently within the camera frame. Images can be acquired using any imaging software and processed either manually off-line or using an appropriate on-line analysis tool such as the Splitview module in Molecular Devices Meta series software; Field Split in Andor’s iQ; or our Optosplit drop-in for ImageJ. The instrument is configured to attach to the c-mount output port of a research microscope, with a c-mount camera fitted to its output and its design allows for connection to a variety of alternative devices, so please consult with us if you intend using it in any other configuration.
Body options P280/310/0LS
Optosplit III ‘LS’ Triple emission image splitter ( x1.0 magnification) includes rectangular input diaphragm, triple calibration cube & two shutter plates. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
P280/310/MLS
Optosplit III ‘LS’ Triple emission image splitter ( x1.0 magnification) includes OptoMask input diaphragm, triple calibration cube & two shutter plates. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
P280/3xx/0LS
Optosplit III ‘LS’ Triple emission image splitter (custom magnification) includes rectangular input diaphragm, triple calibration cube & two shutter plates. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
P280/3xx/MLS
Optosplit III 'LS' Triple emission image splitter (custom magnification) includes OptoMask input diaphragm, triple calibration cube & two shutter plates. Longer focal ratio lenses optimised for large format cameras up to 16mm x 16mm (e.g sCMOS sensors)
Accessories P290/000/200
Cairn emission / excitation filter cube for 25mm filters (Empty)
P290/BYP/002
Cairn emission filter block for single channel operation (100% through centre channel)
P290/CR3/012
Optosplit III corrector lens kit (Includes 2 full width component holders)
P290/ND3/006
Optosplit III neutral density kit (Includes 2 half width component holders and 4 ND filters)
P290/POL/001
Cairn emission / excitation filter cube with integrated polarising beamsplitter cube
P290/POL/002
Rotating Optosplit auxillary component holder with 25mm polariser (Full width)
P290/AUX/012
Auxiliary component holder for 25mm filters, lenses or apertures for OptoSplit and microscope couplings (full width)
tel: + 1.802.881.0302 www.cairn-research.co.uk
email:
[email protected]
toll free 1.877.417.8313
www.89north.com 11/14
