Transcript
STANDARD OPERATING PROCEDURE Procedure: School/Department: SOP prepared by: Version:
DNA or RNA extraction using spin columns School of Molecular Biosciences Angela Nikolic and Nick Coleman SMB011.3
Section 1 - Personal Protective Equipment 1. Lab coat or lab gown (buttoned up) 2. Nitrile or latex gloves 3. Proper enclosed footwear 4. Safety glasses (at stages where ‘binding buffer’ is used) Section 2 – Potential Hazards + Safety precautions 1. Eye damage if corrosive chaotropic salts contact eyes. Wear safety glasses. 2. Poisoning if chaotropic salts ingested. 3. Skin damage if chaotropic salts contact bare skin. Wear PPE. 4. Environmental damage if chaotropic salts disposed of incorrectly. Do not put spin column waste down the sink – it should be disposed of by autoclaving (if recombinant) and then as hazardous chemical waste (via Room 225). 5. Environmental damage if recombinant DNA is disposed of incorrectly. 6. Injury due to incorrect use of centrifuge. Do not use the centrifuge unless an experienced user, preferably the equipment custodian, has given you a thorough demonstration of how to use it correctly and safely. 7. Fire or electrocution due to centrifuge malfunction. Check the rotor for signs of wear and tear before use, and retire rotors that are excessively scratched or damaged (check with the equipment custodian). Do not exceed speed rating of the rotor. Ensure rotor is firmly attached to the spindle before starting machine. 8. Workers with pre-existing medical conditions (e.g. allergy, immunocompromised state, chemical sensitivity) and workers who are pregnant or expecting pregnancy must consult with their supervisor AND medical specialist AND the university's WHS services before performing this procedure. If there are any serious concerns expressed by any of these individuals, this task must not be performed. Section 3 – Procedure 1. Follow procedure as outlined in the manual provided in your spin column kit. Section 4 – Disposal / Spills / Incidents 1. Do not put spin column waste down the sink – dispose by autoclaving (if recombinant DNA present) or as hazardous chemical waste (via Room 225). 2. Wipe up spills with paper towel, dispose of with autoclave waste. 3. In event that binding buffer gets in eyes, flush with copious amounts of water at eyewash station (>15 min), then seek medical attention. 4. If binding buffer contacts skin, wash hands thoroughly and immediately. 5. Any injuries, incidents or near misses (dangerous situations not resulting in an incident) must be reported to your supervisor and via the online reporting system Section 6 – References and Safety data sheets 1. Read the kit instructions carefully before use, including SDS’s for any hazardous components (typically this is the binding buffer, which is either guanidine thiocyanate, guanidine HCl, or sodium perchlorate). 2. Risk assessment and SOP “Disposal of chemical waste” (SMB008).
Creation date: 25/10/2012 Last review date: 2/3/2015
Next review due: 2/3/2017
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SOP Consultation, Training and Approval Print names and enter signatures and dates to certify that the persons named in this section have been consulted/trained in relation to the development and implementation of this Standard Operating Procedure. WHS Representative (WHS Committee) certifies that consultation has taken place. Position Supervisor
Name
Signature
Date
employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student employee / student
Name Authorising (Printed): DIANNE FISHER .......................................................
Signature: ..............................................................Date: 2/3/15 .............................. WHS Committee Representative Name (Printed): MARKUS HOFER ...................
Signature: ..............................................................Date: 2/3/15 .............................. Creation date: 25/10/2012 Last review date: 2/3/2015
Next review due: 2/3/2017
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