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SPECIFICATIONS FOR UPRIGHT RESEARCH FLUORESCENCE & PHASE CONTRAST MICROSCOPE WITH CCD/C-MOS CAMERA & SOFTWARE 1) Microscope: Trinocular Microscope with Universal infinity corrected (UIS 2) system or equivalent, Built in Kohler illumination for transmitted light with 6V/30W halogen bulb (precentered) or higher, Light intensity manger switch preferred. Day light filter. Vertical stage movement 25mm stage stroke with coarse adjustment limit stopper or equivalent or higher, Torque adjustment for coarse adjustment knobs, stage mounting position variable, High sensitivity fine focussing knob (minimum adjustment gradations 1µm preferred). Interchangeable reversed quintuple revolving nosepiece for five objectives or better. Trinocular tube, F. N. 22 or better, three position prisms for selection of light path is preferred with 100% for observation, 20% - 80% Camera Port – observation and 100% for Camera Port. A pair of 10X eyepieces with minimum F. N 22 Ceramic coated coaxial stage with left or right hand low drive control with rotating mechanism and torque adjustment mechanism or with equivalent technology. Specimen holder for minimum 2 slides. Turret type condenser for Bright field, phase contrast and dark field studies N.A 1.1 or equivalent Plan achromat objectives Plan achromat phase contrast objective 10X with N.A 0.25 or equivalent Plant achromat phase contrast objective 40X with N.A 0.65 or equivalent Plan fluorite / semi apochromat phase contrast objective 100X oil immersion with N.A 1.3 or better 2) Fluorescence attachment: 6-8 position turret based fluorescence illuminator attachment 100-130W mercury or equivalent illumination system (UV excitation) for fluorescence studies 6%, 25% ND filters for fluorescence Fluorescence filter cubes suitable for DAPI, FITC, TRITC and Rhodamine 3) At least 5 Megapixel Color CCD/CMOS camera suitable for both bright field and fluorescence application 5MP colour digital CCD/CMOS camera for microscope, Single chip colour CCD/CMOS camera, 2/3 inch colour CCD/CMOS, 5 megapixels or better, Progressive scanning preferred, RGB primary colour on-chip filters, Minimum Recording area 8.4(H) × 6.6 (V) mm or better, diagonal length 10.75 mm or equivalent, Exposure control Auto/Manual AE lock (enabled when Auto Exposure is selected) Continued on page-2
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4) Image analysis software Proprietary Imaging and analysis software with features like User experience customization, overlay multiple images, Movie playback, Tile view, Snap/ movie acquisition, Time lapse at specified interval, Manual multiple image alignment, Manual EFI imaging, Image processing- Geometry/combine/filter, Region and line measurements, Interactive measurements 5) Image acquisition: Computer with i5 processor, 6GB RAM, 1TB hard disk and suitable configurations to be provided by the supplier along with microscope 6) 2KVA UPS with 100AH tubular battery 7) Minimum one year comprehensive onsite warranty
