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UGA-40 Rapp OptoElectronic
Point Illumination System for Photomanipulation The UGA-40 system offers precise, fast moveable and easily programmable point illumination, integrating galvanometer scanner technology with the use of lasers as light sources. The system consists of an optical module, a microcontroller unit, a camera or DVI frame grabber and a computer with scanner control software. The lasers are application specific and can be ordered separately. In addition to point illumination, regions of interest (ROI) can be designed and scanned. Real-time illumination at desired locations is possible in the "click and fire" mode. Features: programmable, computer controlled illumination of points and ROIs spot size dependent on the microscope model, objective magnification and optical fiber diameter real-time photomanipulation via the click and fire mode sequential illumination of points and/or ROI with user defined positions and temporal settings switching time between any two positions in the field of view: < 1ms simultaneous point/ROI illumination and imaging data acquisition TTL synchronization of sequences or sequence elements with other equipment analog control of laser intensity (when used with lasers provided by Rapp OptoElectronic) Cameras supported by the Rapp OptoElectronic software: Hamamatsu Orca series, Zeiss AxioCam MRM, Photometrics CoolSnap HQ, Jenoptik ProGres MF, Eye industrial USB camera ● light sources: solid state UV, VIS and near-IR lasers ● ● ● ● ● ● ● ● ●
Scanner Module ● ● ● ● ● ● ● ● ● ●
Add-on module, can be integrated into existing set-ups Switching time between any two spot locations < 1ms Scanning speed within an ROI up to 5kHz Spot size as low as submicron, depending on microscope, objective and light guide aperture Wide variety of UV, VIS and NIR lasers available (266 to 1535 nm) Up to four lasers per system Switching time between two laser lines: < 1ms Laser coupling to the system: direct or via light guide Can be combined with wide field fluorescence, LSM and spinning disc confocal, etc. Modules available for most modern research microscope brands and models
Software Features UGA-40 software runs in parallel with and independent of any other imaging or electrophysiology software ● Simultaneous and independent spot illumination and imaging data acquisition ●
BioMedical Instruments Zur Schoenen Aussicht 26 D-07751 Zoellnitz Germany
phone: +49 3641 395985 fax: +49 3641 478178 mail:
[email protected] web: www.biomedical-instruments.com
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"Click & Fire" mode for real time spot illumination Programmable illumination (sequences) of multiple locations and/or regions of interests (ROIs) ROI geometries include: lines, circles, polygons, free-hand drawn shapes Grid stimulation with sequential or random illumination of grid points TTL-in and -out Manual or TTL-triggered sequence start TTL-triggered events within the sequence Able to run up to two lasers within the same experiment
BioMedical Instruments Zur Schoenen Aussicht 26 D-07751 Zoellnitz Germany
phone: +49 3641 395985 fax: +49 3641 478178 mail:
[email protected] web: www.biomedical-instruments.com