Ymc-triart Prep

Transcript

YMC-Triart Prep Robust and pH stable hybrid silica process material The Selectivity Company Robust Improved particle properties result in better chromatographic performance Flexible Chemical stability pH 1 - 12 Durable against alkaline CIP Economic Purify more endproduct on less stationary phase www.ymc.de 2 YMC-Triart Prep Particle Technology................................................................................................................................... page 3 Physicochemical Characteristics...................................................................................................... page 4-5 Mechanical Stability.................................................................................................................................. page 6 Comparing YMC-Triart Prep C18-S to competitor material....................................................... page 7 Cleaning-in-place (CIP)......................................................................................................................... page 8-9 Applications ............................................................................................................................................... page 10 Ordering Information............................................................................................................................. page 11 Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 3 YMC-Triart Prep With YMC-Triart Prep, a pH stable preparative grade HPLC stationary phase gets available. It is produced in lot sizes of up to several hundred kg. This innovative multi-layer hybrid material sets a new benchmark for RP HPLC phases. It is well suited for production scale applications, as it is available up to multi-ton scale. YMC-Triart Prep is chemically stable up to pH 12.0. This provides more flexibility for method development and allows for more efficient cleaning-in-place (CIP) procedures. From real life process development work YMC-Triart Prep has shown to outperform traditional silicabased materials 2- to 4-fold in terms of durability. Longer column lifetimes lead to more kilogram of product produced per kilogram of stationary phase. The results are: improved production procedures and reduced overall costs. Particle technology YMC-Triart Prep is a multilayered material prepared from a well-controlled particle formation process which is adapted from micro-reactor technology. The recently developed production step results in truly narrow particle and pore size distributions! With YMC-Triart Prep otherwise, challenging pH and high temperature conditions are no longer a limitation even to the day-to-day work in laboratories or production. Most importantly, due to its unique particle composition, a balanced hydrophobicity and silanol activity are achieved which makes YMC-Triart Prep a ”First Choice“ material in method development! YMC-Triart Prep hybrid structure inorganic siloxane layer organic siloxane layer Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 4 Specification YMC-Triart Prep C18-S YMC-Triart Prep C8-S Base material inorganic/organic multi-layered hybrid Particle size [µm] 10,15, 20 10, 15, 20 Pore size [nm] 12 20 Specific surface area [m2/g] 360 220 polymeric C18 polymeric C8 yes yes 1.0 ~ 12.0 1.0 ~ 12.0 Bonding End-capping pH range Homogeneous and uniform particles* YMC-Triart YMC-Triart Prep X-Bridge HILIC (12 nm, 5 µm) (20 nm, 15 µm) (13.5 nm, 5 µm) ( TM of Waters Corp.) YMC-Triart Prep is produced using micro-reactor technology in the granulation process. The results are spherical particles with a highly uniform particle and pore size distribution. This leads to a reduction of the back-pressure and to more reproducibility in surface modification. * by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 5 Excellent pH stability Alkaline condition (pH 11.5, 50 °C) 100 YMC-Triart Prep C18-S, 15 µm 80 Kromasil C18, 16 µm (Kromasil) 60 ODS-AP, 15 µm (Daiso) 40 Chromatorex C18, 15 µm (Fuji) 20 0 The columns are stored in acetonitrile/water/TFA (10/90/1) 70 °C, and the performance tested every 20 hours. 20 0 Column: Eluent: Flow rate: Temperature: Detection: Sample: 40 60 Time (hr) 80 (%) of initial theoretical plate (%) of initial retention Acidic condition (pH 1, 70 °C) 80 60 Chromatorex C18, 15 µm (Fuji) ODS-AP, 15 µm (Daiso) 40 20 0 100 250 x 6.0 mm ID acetonitrile / water (60/40) 1.7 ml/min 37 °C UV at 254 nm butyl benzoate YMC-Triart Prep C18-S, 15 µm 100 0 100 Kromasil C18, 16 µm, Kromasil 200 Time (hr) 300 Column: 150 x 4.6 mm ID Eluent: 50 mM TEA in methanol / 50 mM TEA in water (pH 11.5) (20/80, v/v) Flow rate: 1.0 ml/min Detection: UV at 254 nm Sample: caffeine At high pH YMC-Triart Prep C18-S shows lifetimes of up to 4 times longer compared to conventional silica materials. This enables new separations at high pH which are not possible with silica material. Furthermore, the material can endure more CIP-cycles than conventional phases used in industrial processes (see page 8). Advantages of YMC-Triart Prep YMC-Triart Prep Your benefit pH-stability 1-12 Flexibility Method development Durable against CIP-conditions Chemical and mechanical stability Cost savings High production throughput Uniform particle size Improved packing = improved chromatographic performance Narrow pore size distribute High loadability Application Data by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 6 Mechanical stability test Test procedure 1. Column packing Column: DAU-50-1000 Bed length: 100 x 50 mm ID Pressure: 6.5 MPa 2. Pressure measurement Eluent: methanol / water (85/15) Flow rate: 50 ml/min Temperature: ambient 3. Unpacking Backpressure Psi/m 2000 Less backpressure = 1500 higher throughput possible 1000 500 0 1 5 Packing number 10 ■ YMC-Triart Prep (10 µm, 20 nm) (Hybrid silica) ■ YMC HG-series (10 µm, 20 nm) (High grade silica) ■ Traditional Silica (10 µm, 20 nm) Shearing and crushing of silica particles leads to the formation of fines, which results in an increased backpressure. By using mechanically stable, spherical particles the formation of fines can be reduced. Here, high mechanical stability of YMC-Triart Prep is demonstrated by means of repeated packing of a DAC column. Even after more than 10 repacking cycles for the same material the pressure does not increase. The absence of fines is proven by a constant backpressure. This makes YMC-Triart Prep a valuable asset for method development and especially for process optimisation! Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 7 YMC Triart Prep C18-S vs. competitor material (client data*) Client data confirms that YMC-Triart Prep C18-S shows retention for hydrophobic compounds comparable to conventional silica materials. At the same time polar molecules are exceptionally well separated. * Client data shows non-optimized scouting runs performed on the respective C18 material packed into 250 x 4.6 mm ID columns. Narrow pore distribution 4500 4000 YMC-Triart Prep d Vp/ d logD 3500 3000 Conventional silica-based particle 2500 2000 X-Bridge HILIC 1500 1000 500 0 0 5 10 15 20 Pore size (nm) 25 30 35 A narrow pore size distribution of a stationary phase beneficially affects peak width and sample loading in liquid chromatography. Where micropores account for an artificially high surface area of a stationary phase but are mostly inaccessible to target molecules, mesopores decreases the column efficiency due to a steric hindrance effect, which significantly slows mass transfer. YMC-Triart Prep C18-S exhibits a narrower pore size distribution with fewer micro- and mesopores. This results in improved peak shapes and higher sample loading in your preparative processes. Application Data by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 8 Cleaning-in-place (CIP) Test procedure Sample injection (insulin in human serum) injected 20 times Regeneration with alkaline solution Wash with alkaline solution 0.1 M NaOH / acetonitrile (50/50) 10 CV Neutralisation and wash with organic solvent 1. acetonitrile / water (20/80) 2. acetonitrile / water (90/10) CIP performance of YMC-Triart Prep C8-S Effect of CIP with alkaline solution Column: Eluent: YMC-Triart Prep C8-S (20 nm, 10 µm) 250 x 4.6 mm ID A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 28-35% B (0-15 min) Durability under repeated CIP Flow rate: Temperature: Detection: Injection: Sample: 1.0 ml/min 25 °C UV at 220 nm 30 µl 10 mg/ml insulin bovine + human serum (2:1) Application Data by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 9 CIP performance of YMC-Triart Prep C8-S YMC-Triart Prep C8-S (10 µm, 20 nm) Column: Eluent: Flow rate: Temperature: Detection: Injection: Sample: 50 x 4.6 mm ID A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 29-36% B (0-2 min), 36% B (2-3 min), 29% B (3-6 min) 1.0 ml/min 25 °C UV at 220 nm 6 µl 10 mg/ml insulin bovine + human serum (2:1) Silica based C8 material (10 µm, 20 nm) e lifetim Column nded exte or > 2 by fact In production repeated injections of a solution of insulin in human plasma/serum are carried out. Consequently, absorption of protein and other impurities to the surface of the packing material reduces the retention capacity of the column. Methodology: At this point a wash step with an alkaline solution (e.g. 0.1 M NaOH) removed the impurities and restores the full capacity of the column. Problem: Silica materials are unsuitable for alkaline wash conditions, because of their poor durability at high pH. Solution: Hybrid silica based YMC-Triart Prep has an excellent durability at high pH. It is amenable to alkaline wash conditions and thus enables longer column lifetime. This in turn reduces production costs: Lower consumption of packing material and fewer downtimes due to column repacking. An extension of column lifetime by a factor of > two has been achieved! Application Data by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 10 Structurally similar peptides at pH > 7 Easy tion separa pH-shift Column: Eluent: Flow rate: Detection: Injection: Sample: YMC-Pack Polymer C18, 250 x 4.6 mm ID acetonitrile / water / TFA (18/82/0.05) (pH 2.2) 0.5 ml/min UV at 220 nm 10 µl 1. Angiotensin III 2. Angiotensin II 3. Angiotensin I Column: Eluent: Gradient: Flow rate: Detection: Injection: Sample: YMC-Triart Prep C8-S (20 nm, 10 µm) 250 x 4.6 mm ID A) acetonitrile / 10 mM NH4H2PO4 + NH4OH (50/50) (pH 2.2) B) 10 mM NH4H2PO4 + NH4OH (pH 7) 35 - 65% A in 25 min 1 ml/min UV at 215 nm 10 µl 1. Angiotensin II 2. Angiotensin III 3. Angiotensin I Methods developed on analytical YMC-Triart material are readily transferred to preparative conditions. Thus, YMC-Triart Prep is equally suitable for industrial scale purifications and in process controlls. Ordering information YMC-Triart Prep, preparative bulk media YMC-Triart Prep C18-S Pore size (nm) Particle size (µm) Product Code 10 TAS12S11 12 15 TAS12S16 20 TAS12S21 Typical pack sizes: Laboratory scale: 100 g - 5 kg PE bottle Industrial scale: 5 kg - 50 kg in double lined PE bags inside metal drum Larger pack sizes on request YMC-Triart Prep C8-S Pore size (nm) Particle size (µm) Product Code 10 TOS20S11 20 15 TOS20S16 20 TOS20S21 Other particle and pore size combinations on request. Examples of existing customised material: TAS08S11, TOS12S11 Application Data by courtesy of YMC Co., Ltd. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de 11 Other available brochures: YMC*Gel HG-series brochure YMC*Gel HG-series High grade silica phases for preparative HPLC The Selectivity Company General purpose preparative phases on high grade silica base. Available as silica or with C18, C8, C4, C1, phenyl, cyano, amino or diol bonding. Particle size: Pore size: 10 µm, 15 µm, 20 µm, 50 µm 8 nm, 12 nm, 20 nm, 30 nm www.ymc.de YMC-BioPro prep brochure Polymeric ion exchange material for biochromatography. YMC-BioPro IEX materials Available as strong anion- or cation-exchanger. Excellent flow properties and high dynamic binding capacity. Prep Bulk media Extremely economic in industrial scale applications. YMC-Triart Prep C8 brochure Purification of Insulin with YMC-Triart Prep C8 Improved insulin purification process with YMC-Triart Prep C8. Maximum output. Lowest sorbent costs. Increased Output at Reduced Costs Lowest overall costs. Highest mechanical stability. Daisogel is a trademark of Daiso Co., Ltd. Kromasil ia a trademark of Akzo Nobel, Separation Products. Schöttmannshof 19 · D-46539 Dinslaken · Phone +49 (0) 20 64 427-0 · Telefax +49 (0) 20 64 427-222 E-Mail: [email protected] · www.ymc.de Your local distributor: Achrom N.V. · Phlippostraat 7 · 9870 Machelen-Zulte BELGIUM Telephone: (+32) 9/380.06.47 · Fax: (+32) 9/386.08.86 E- mail: [email protected] · Internet: www.achrom.be Europe GmbH Co., Ltd. YMC Karasuma-Gojo Bld. 284 Daigo-cho, Karasuma Nisiiru Gojo-dori Shimogyo-ku, Kyoto 600-8106 Japan TEL. +81(0)75-342-4515, FAX +81(0)75-342-4550 www.ymc.co.jp Taiwan Co., Ltd. 3F, No. 1353, Zhongzheng Rd., Taoyuan City, Taoyuan Country 330, Taiwan (R.O.C.) TEL. +886-3-2150-630, FAX +886-3-2150-286 www.ymctaiwan.com Schöttmannshof 19 D-46539 Dinslaken Germany TEL. +49(0)2064/427-0, FAX +49(0)2064/427-222 www.ymc.de Korea Co., Ltd. #208, Owners Tower, 16-5, Sunae-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-825 Korea TEL. +82-31-716-1631, FAX +82-31-716-1630 www.ymckorea.com Co., Ltd. Shanghai Rep. Office Far East International Plaza A2404 No. 319 Xianxia Road, Shanghai 200051 P.R. China TEL. +86-21-6235-1388, FAX +86-21-6235-1398 www.ymcchina.com America, Inc. 941 Marcon Boulevard Suite 201 Allentown, PA18109 USA TEL. +1-610-266-8650, FAX +1-610-266-8652 www.ymcamerica.com India Pvt. Ltd. A-154, Eros Boulevard Hilton Hotel Complex Plot No.13-B, District Centre Mayur Vihar Phase-I New Delhi-110091 India TEL. +91-11-45041601;45041701, FAX +91-11-45041901 www.ymcindia.com Singapore Tradelinks Pte. Ltd. 6 Indus Road, #13-09 Emerald Park, Tower 1, Singapore 169588 TEL.+65-9023-7617 www.ymc.sg YMC-Triart Prep 09/2014_EN